이상지질혈증과 치료제 연구개발 경향

설인찬 ( In Chan Seol ) 대전대학교 한의학연구소 2009 한의학연구소 논문집 Vol.18 No.2

Most of the cholesterol is synthesized by liver in the body while about one of third is taken via dietary. The main functions of cholesterol is to protect membranes in cell surface, avoid the arterial bleeding by hypertension, and prolong the life of erythrocytes, and so on. However, overload of cholesterol leads to arteriosclerosis associated with leading death cause. Lack of physical activity, emotional and environmental stress, and low intake of protein or vitamin E induce the unbalance between HDL- and LDL-cholesterol so become a basis of ischemic disorders in heart, brain and elsewhere in the body. So far, four major classes of medications for hyperlipidemia are HMG-CoA reductase inhibitors (statins), bile acid sequestrants, nicotinic acid, and fibric acids. The statins can lower LDL and levels triglyceride, but may induce myopathy and an elevation of liver enzyme levels. The bile acid sequestrants lower LDL levels and raise HDL levels with no effect on triglyceride levels but side effects of gastrointestinal (GI) distress, constipation, and a decrease in the absorption of other drugs. Nicotinic acid and fibric acids lower LDL and triglyceride levels with showing flushing, hyperglycemia, hyperuricemia, GI distress, and hepatotoxicity dyspepsia, gallstones, myopathy, and unexplained noncardiac death as adverse effects. Above western drugs lower cholesterol by 15 to 30% while all have notable adverse effects. In traditional medicine, hyperlipidemia is regarded as retention of phlegm and fluid disease. Etiology and pathogenesis of hyperlipidemia is basically based on Spleen-Deficiency and Phlegm-Stagnation, accumulation and stasis of -heat, and Qi & blood stagnation induced by Phlegm-damp, water-dampness, and blood stasis. Thereby, strengthening Spleen and dissolving Phlegm, clearing away heat and diuresis, and supplementing Qi and activating blood circulation are commonly used therapeutic methods for hyperlipidemia. The traditional herbal medicine, have been used for patients with CVA, hypertension or hyperlipidemia in Oriental hospital or Oriental clinic. The lock and key theory is used to develop most of western medicine, however many diseases are caused by mixed factors in body-complex system. We expect that Oriental pharmacological theory could be newborn as a novel drug showing high advantage of blood levels of lipidsand QOL of performance without side effects.

어깨통증의 한의학적 치료에 대한 국내 임상 연구 고찰

김찬영(Chan-young Kim),양지혜(Ji-hye Yang),채인철(In-cheol Chae),최인우(In-woo Choi),유주영(Ju-young Ryu),정은선(Eun-sun Jung),김윤식(Yoon-sik Kim),설인찬(In-chan Seol),유호룡(Ho-ryong Yoo) 대한한의학회 2020 대한한의학회지 Vol.41 No.3

db/db mouse에서 인진(茵蔯), 울금(鬱金), 산사(山査) 복합추출물의 항고지혈 및 항산화 효과

김현태,김윤식,설인찬,유호룡,Kim, Hyun-tae,Kim, Yoon-sik,Seol, In-chan,Yoo, Ho-ryong 대한한방내과학회 2016 大韓韓方內科學會誌 Vol.37 No.3

Objective: This study was performed to investigate the effect of IUS (Inulsan, an extract of Artemisiae capillaris (茵蔯), Curcumae longae (鬱金), and Crataegi fructus (山査)) on anti-hyperlipidemia, anti-oxidation, and anti-inflammation.Method: We administered water extracts of Artemisiae capillaris, Curcumae longae, and Crataegi fructus for three weeks to db/db mice (C57BL/Ks), animal models induced with type 2 diabetes mellitus. Mice were divided into three groups: normal (C57BL/6J mice group), control group (db/db mice without administration of IUS) and IUS group (db/db mice treated with IUS). Then we measured total cholesterol, LDL cholesterol, HDL cholesterol, and triglyceride in the serum after the oral administration of IUS.Results: 1. IUS did not show any cytotoxicity in RAW 264.7 cells. 2. IUS decreased AST, ALP, and creatinine levelsand did not show any liver or renal toxicity in the db/db mice. 3. IUS increased DPPH and ABTS radical scavenging activity and decreased ROS production in RAW 264.7 cells. 4. IUS significantly decreased IL-1β, IL-6, and TNF-α production in RAW 264.7 cells. 5. IUS increased HDL cholesterol and significantly decreased total cholesterol and triglyceride in db/db mice. 6. IUS significantly decreased the atherogenic index and cardiac risk factor. 7. In contrast with the control group, fat infiltration in the liver and aorta decreased in IUS treated mice. The cell nucleus was located in the central area in H&E staining of liver. And endomembranes also were more thinner than the control group in H&E staining of aorta.Conclusions: These results suggest that IUS might be effective in the prevention and treatment of dyslipidemia.

가미자음강화탕(加味滋陰降火湯)이 SHR의 혈압에 미치는 영향

백경민,조현경,유호룡,김윤식,설인찬,Baek, Kyung-Min,Jo, Hyun-Kyung,Yoo, Ho-Rhyong,Kim, Yoon-Sik,Seol, In-Chan 대한한방내과학회 2006 大韓韓方內科學會誌 Vol.27 No.1

Objective : This study was done to investigate the effect of KJT on hypertension. Methods : Spontaneous Hypertensive Rats were sensitized and challenged with Kamijaeumganghwatang(KJT) for 5 weeks. Experimental group was treated with $200mg/day/2m{\ell}$ of KJT orally and control group was treated with $2m{\ell}/day$ of normal saline instead. Results : 1. KJT significantly showed significant protection against cytotoxicity and toxicity in the liver and the kidney. 2. KJT significantly decreased the blood pressure in SHR. 3. KJT significantly decreased the levels of aldosterone in SHR. 4. KJT significantly decreased the levels of dopamine, norepinephrine and epinephrine in SHR. 5. KJT significantly decreased the levels of sodium and potassium in SHR, but it did not decrease the levels of chloride in SHR. Levels of calcium in SHR increased, but only insignificantly. 6. KJT significantly decreased the levels of IL-6 in SHR, and significantly increased the levels of IL-10 in SHR. 7. For histological effects, KJT dillated capillary vessels in the kidney, significantly decreased eosinophilic changes in heart cells, and significantly decreased endothelial damage in the aorta. Conclusion: These results suggest that KJT is useful in treatment of hypertension.

한의학(韓醫學)에서 분류하는 사상체질(四象體質)에 따른 이식항원(移植抗原) HLA-DR 유전자(遺傳子) polymorphism에 관한 연구

권택부(Taek Boo Kwon),황치원(Chee Won Whang),설인찬(In Chan Sul),이경옥(Kyung Ok Lee),황규원(Kyu Yoon Hwang),양동호(Dong Ho Yang),홍세용(Sae Yong Hong) 대한내과학회 2000 대한내과학회지 Vol.58 No.1

N/A Background : In Korean traditional medicine, human beings are classified into 4 groups accordance with the characteristics of physical constitution, named ; Taeum, Taeyang, Soum, and Soyang. Most of Korean traditional doctors believe that the back ground of pathophysiology is differ among each group in many diseases. HLA is the most polymorphic gene and related with human immune response. It has been used for determination of individual identification, genetic diversity and a study of disease association in different ethnic group. It has well known that the most human inflammatory disease which thought to have some autoimmune basis are in some way promoted by genes of the HLA region. The purpose of this study is to observe the distribution of HLA-DR genotypes in 4 types of physical constitution in Korean traditional medicine. Methods : Three hundred and ten Korean subjects(Soyang : 58cases, Soum :142cases, Taeum : 92cases, Taeyang : 18cases) were enrolled for this study. Genomic DNAs extracted by phenol/chroloform were amplified with allele specific primers and determined by 2% agarose gel electrophoresis with ethidium bromide staining for analysis of HLA-DR polymorphism. Results : The genotype frequency of HLA-DR in a total or 310 Koreans was DR1 ; 7.2%, DR 2; 10.5%, DR 3;1.7%, DR 4; 19.9%, DR 5; 11.9%, DR 6;19.9%, DR 7; 7.0%, DR 8; 10.6%, DR 9; 8.7%, DR10;1.9%. There was no significant diffrence of HLA-DR genotype distribution among four physical constitution groups classified by Korean traditional medicine. Conclusion : Our results suggest that the classification of physical constitution in Korean traditional medicine is not related to HLA-DR genotypes.(Korean J Med 58:91-101, 2000)

가미지황탕(加味地黃湯)이 고지혈증(高脂血症) 관련(關聯) 인자(因子)에 미치는 영향

박소애,조현경,유호룡,김윤식,설인찬,안정조,Park, So-Ae,Jo, Hyun-Kyung,Yoo, Ho-Ryong,Kim, Yoon-Sik,Seol, In-Chan,An, Jung-Jo 대한한방내과학회 2009 大韓韓方內科學會誌 Vol.30 No.2

Objective : Gamijihwang-tang(GJT) has been used as a therapeutic agent for hyperlipidemia in oriental medicine for several years. This study was performed to investigate the effects of GJT on hyperlipidemia in rats using diverse biological methods. Method : Hyperlipidemia was induced by a hyper-lipidemic diet fed for 4 weeks. Total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, and glucose were measured in the serum after an oral administration of GJT. Lipid peroxidase, SOD, catalase, ACAT, and HMG-CoA were measured in liver after oral administration of GJT. Result: 1. GJT showed safety against cytotoxicity and toxicity in liver. 2. GJT significantly decreased rat's body and liver weight. 3. GJT significantly decreased serum total cholesterol and LDL-cholesterol, but increased serum HDL-cholesterol. 4. GJT significantly decreased serum triglyceride and glucose. 5. GJT significantly decreased lipid peroxidation and increased SOD and catalase in liver. 6. GJT significantly decreased ACAT and HMG-CoA reductase of cholesterol manifestation in liver. Conclusions : These results suggest that GJT might be effective in treatment and prevention of hyperlipidemia.

고cholesterol 식이 자발성 고혈압 백서(白鼠)에서의 가미방풍통성산(加味防風通聖散)이 고혈압에 미치는 영향

김종원,조현경,유호룡,설인찬,김윤식,Kim, Jong-Won,Jo, Hyun-Kyung,Yoo, Ho-Rhyong,Seol, In-Chan,Kim, Yoon-Sik 대한한방내과학회 2006 大韓韓方內科學會誌 Vol.27 No.3

This experiment was performed to determine the effects of Kamibangpungtongsungsan on hypertension in spontaneous hypertensive rats fed a hyperlipidemic diet (H-SHR). The results are summarized as follows : 1. Kamibangpungtongsungsan significantly decreased the blood pressure and pulse of rats in H-SHR. 2. Kamibangpungtongsungsan significantly decreased the levels of Aldosterone in H-SHR. 3. Kamibangpungtongsungsan significantly decreased the levels of dopamine and epinephrine in H-SHR, but did not significantly reduce the levels of norepinephrine, in H-SHR. 4. Kamibangpungtongsungsan did not significantly reduce the levels of electrolytes in H-SHR. 5. Kamibangpungtongsungsan significantly decreased the TNF-$\alpha$ and IL-6 levels in H-SHR but the IL-10 level increase no significant. These results suggest that the Kamibangpungtongsungsan might be usefully applied for the treatment of hypertension with hyperlipidemia.

가미청열도담탕(加味淸熱導痰湯)이 DOCA-salt로 유발된 고혈압 백서에 미치는 영향

백혜기,안정조,조현경,유호룡,김윤식,설인찬,Baek, Hye-Ki,An, Joung-Jo,Jo, Hyun-Kyung,Yoo, Ho-Ryong,Kim, Yoon-Sik,Seol, In-Chan 대한한방내과학회 2008 大韓韓方內科學會誌 Vol.29 No.3

This study was done to investigate the effect of Gamichungyeoldodam-tang (GCDT) in DOCA-salt induced hypertensive rats. The results were obtained as follows : GCDT showed safety against cytotoxicity and toxicity in the liver and the kidney. GCDT showed an inhibitory effect on ACE. GCDT significantly decreased the heart rate and the blood pressure in DOCA-salt induced hypertensive rats. GCDT significantly decreased the levels of aldosterone in DOCA-salt induced hypertensive rats. GCDT significantly decreased the levels of dopamine, norepinephrine, and epinephrine in DOCA-salt induced hypertensive rats. GCDT significantly decreased the levels of chloride in DOCA-salt induced hypertensive rats. These results suggest that GCDT might be effective in treatment of hypertension.

인진(茵蔯), 울김(鬱金), 지실(枳實) 추출물(ACA)이 HepG2 세포에서 나타나는 이상지질혈증 관련 인자 발현 및 항산화에 미치는 영향

유주영,조현경,유호룡,설인찬,김윤식,Ryu, Ju-young,Cho, Hyun-kyoung,Yoo, Ho-ryong,Seol, In-chan,Kim, Yoon-sik 대한한방내과학회 2017 大韓韓方內科學會誌 Vol.38 No.3

Objective: To investigate the effect of Artemisiae Iwayomogii Herba, Curcumae Radix, and Aurantii Fructus Immaturus complex extract (ACA) on dyslipidemia-related factor expression and anti-oxidation in HepG2 cells. Method: After treatment with ACA in the HepG2 cells, DPPH, ABTS radical scavenging activity, ROS production, and glutathione (GSH) production were measured. The free fatty acid, lipid peroxidation (MDA), ACAT1, and HMG-CoA reductase mRNA expression were measured in the HepG2 cells after treatment with ACA. Results: 1. DPPH, ABTS radical scavenging activity increased in an ACA concentration-dependent manner. 2. ACA significantly decreased ROS production in comparison to the control group. 3. ACA significantly increased glutathione production. 4. ACA significantly decreased free fatty acid and lipid peroxidation (MDA) in the HepG2 cells. 5. ACA decreased the mRNA expression of ACAT1 and HMG-CoA reductase. Conclusion: These results suggest that Artemisiae Iwayomogii Herba, Curcumae Radix, and Aurantii Fructus Immaturus complex extract (ACA) inhibits dyslipidemia-related factor expression and that it is effective in anti-oxidation. A future in vivo experiment with ACA is needed to investigate the effect on anti-dyslipidemia. It is expected that ACA is effective in anti-dyslipidemia and applied to cardiovascular disease, ischemic heart disease, stroke, etc.

한국형 중풍 변증 표준시안의 습담 변증 지표에 대한 연구

조현경,김중길,강병갑,유병찬,백경민,이인,최선미,설인찬,Jo, Hyun-Kyung,Kim, Joong-Kil,Kang, Byeong-Kab,Yu, Byeong-Chan,Baek, Kyung-Min,Lee, In,Choi, Sun-Mi,Seol, In-Chan 대한한방내과학회 2006 大韓韓方內科學會誌 Vol.27 No.1

Objectives : This study was done to investigate clinical frequency and correlation among the indicators of dampness-phlegm pattern identification settled by tentative Korean Standard Differentiation of the Symptoms and Signs for Stroke. Methods : The subjects were 147 hospitalized patients with stroke, and a list of registry was made for each of them. Among the five types of pattern identification, fire-heat, dampness-phlegm, blood-stasis and deficiency of Qi and of Yin, those that have shown a high frequency in dampness-phlegm type were categorized as the dampness-phlegm pattern group. Frequency of dampness-phlegm indicators was compared with those from the non-dampness-phlegm pattern group. Correlations among dampness-phlegm indicators were also studied. Results : 1. Dampness-phlegm pattern group included 26 patients out of 147. 2. Among the indicators of dampness-phlegm pattern. those, in order of highest frequency, were 'tiredness or sluggishness', 'white Coated tongue' and 'sputum'. 3. In comparing dampness-phlegm pattern group with non-dampness-phlegml group, the indicators such as 'lightheadedness', 'nigrescence', 'sputum', 'dermatic dysaesthesia' showed significantly high frequency. 4. Among the indicators, 'sputum' and 'tiredness or sluggishness', 'sputum' and 'yellow coated tongue', and 'white coated tongue' and 'yellow coated tongue' showed significant correlation. 5. In investigation of the correlation of scale in symptoms, various results such as positive correlation and negative correlation were obtained. Conclusion : In this study, more sensitive indicators of dampness-phlegm pattern identification were found. Based on these results, it is suggested that a more practical Korean Standard Differentiation of the Symptoms and Signs of Stroke would be established through continuous clinical studies by giving weight on each specific type of pattern identification.