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새로이 분리된 Klebsiella pneumoniae 균주들의 글리세롤 기반 3-hydroxypropionic acid 및 1,3-propanediol 동시 생산성 평가
고연주(Yeounjoo Ko),설은희(Eunhee Seol),순달아 세칼 바라지(Balaji Sundara Sekar),권성진(Seongjin Kwon),이재현(Jaehyeon Lee),박성훈(Sunghoon Park) 한국생물공학회 2016 KSBB Journal Vol.31 No.4
Co-production of 3-hydroxypropionic acid (3-HP) and 1,3-propanediol (1,3-PDO) was suggested as an innovative strategy to overcome several limitations occurring in the single production of 3-HP from glycerol. In this study, two new isolates of Klebsiella pneumoniae, which produce less lipopolysaccharide (LPS) thus considered less pathogenic than K. pneumoniae DSM 2026, were compared and evaluated for their potential for the co-production of 3-HP and 1,3-PDO. The newly isolated strains showed significantly faster sedimentation rate than DSM, which should be beneficial for downstream processing. Analysis of genome sequences of the isolates confirmed the presence of all genes necessary for glycerol assimilation, 1,3-PDO production and biosynthesis of coenzyme B12. Co-production yield was highest under anaerobic condition while cell growth was highest under aerobic condition. Both strains showed similarly good performance for the co-production although J2B gave the slightly higher co-production yield of 0.80 mol/mol than GSC021 (0.75 mol/mol). The evaluation of the newly developed strains presented here should be useful in designing similar evaluation experiments for other microorganisms.