NIH3T3 세포에 주입된 Taxol-Like Protein-35 의 작용 및 세포독성

선우재근(Jae Gun Sunwoo),이진우(Jin Woo Lee),황병두(Byung Do Hwang) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.4

N/A Objective : To know whether the effects of TALP-35 introduced into NIH3T3 cells by osmotic lysis of pinosome (OLP method) can associates with microtubule, increases microtubule polymerization in cells and induces cytotoxicity. Methods : By osmotic lysis of pinosome (OLP method), TALP-35 was added to NIH3T3 cell. thereafter, distribution of TALP-35, indirect immunofluorescence microscopy, analysis of cells were done. Results : When TALP-35 was added to NIH3T3 cell homogenate, tubulin was cosedimented with TALP-35 and presented in microtubule pellets. TALP-35 was associated with microtubules and increased microtubule polymerization in NIH3T3 cells. Internalization of TALP-35 caused cell death. Cytotoxicity was found to be caused by apoptosis and G2/M phase block. Conclusion : TALP-35 infused in cell made microtubule polymerization increasing, stable and formed microtubule bundle. The mechanism of TALP-35-dependent was presented in cytotoxicity in NIH3T3 cell.

자궁경부의 악성혼합뮐러종양

선우재근 ( Jae Gun Sunwoo ),조인숙 ( In Sook Cho ),전섭 ( Seob Jeon ),배동한 ( Dong Han Bae ),신영우 ( Young Woo Shin ),김창진 ( Chang Jin Kim ),정동준 ( Dong Jun Jeong ) 대한산부인과학회 2008 Obstetrics & Gynecology Science Vol.51 No.3

Malignant mixed mullerian tumors (MMMT) are rare biphasic malignant neoplasm with two components of carcinoma and sarcoma. The most common site of occurrence in female genital tract is the uterine corpus. MMMT of the cervix is extremely rare. MMMT of uterine corpus is highly malignant and the prognosis is poor. Surgery is treatment of choice of uterine cerivx MMMT. The clinicopathologic characteristics of tumor and treatment are uncertain because of lack of clinical data. We experienced one case of malignant mixed mullerian tumor of uterine cervix and report our experience with a brief review of literature.

전자궁적출술 후 항문-직장기능에 미치는 영향에 관한 연구

선우재근(Jae Gun Sunwoo),최규연(Kyu Yeon Choi),김민관(Min Kwan Kim),이슬기(Seul Ki Lee),배동한(Dong Han Bae),이문호(Mun Ho Lee) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.8

목적: 전자궁적출술은 변비와 같은 장기능에 영향을 미치는 것으로 알려져 있으므로 전 자궁적출술후 나타나는 항문-직장의 생리변화를 평가하기위해 전향적 연구를 시행하였다. 연구방법: 악성종양이 아닌 부인과 질환으로 전자궁적출술을 시행받은 14명을 대상으로 수술 전후 설문지 조사 및 항문-직장 내압검사를 시행하고 수술 시행 2개월 후 다시 설문지 조사와 항문-직장 내압검사를 시행하였다. 자료 분석은 Paired t-test를 이용하였다. p-value가 0.05 미만일 때 통계적으로 유의한 차이가 있다고 판단하였다. 결과: 복식 전자궁적출술 시행받은 14명에서 수술 시행 전에는 소직경 대변을 보인 환자가 없었으나 수술후 한 명이 소직경 대변을 보였고 항문-직장 내압검사는 수술 전후 통계학적으로 유의한 차이가 없었다. 풍선 배출 검사는 수술 시행전 모든 대상에서 양성이었으나 수술 시행후 4 명(29%)에서 풍선 배출 장애를 보였다. 결론: 전자궁적출술은 항문-직장압과 직장 감각능에는 영향을 미치지 않으나 일부환자에서 직장 배출능력의 장애가 나타날 수 있으므로 경과관찰이 필요할 것으로 생각된다. Objective: It has been suggested that hysterectomy has a disturbing influence on bowel function, mainly constipation. We performed a prospective study to assess the changes of ano-rectal physiology after hysterectomy. Methods: Fourteen consecutive patients were assessed before and two months after hysterectomy. A detail questionnaire was devised to allow assessment of bowel function and ano-rectal pressure test and balloon expulsion test were performed before and after hysterectomy. The parameters measured in ano-rectal pressure test included the minimal sensible volume, ano-rectal resting pressure, maximal squeezing pressure, recto-anal inhibitory reflex and balloon expulsion test. Data analysis was carried out by paired t-test. Statistical significance was inferred when the p value was〈0.05. Results: Among the fourteen patients, the straining in defecation was found in three patients after operation, other defication habits were not significantly changed after hysterectomy(P>0.05). There were no significant changes in ano-rectal pressure test after hysterectomy. The disturbance of balloon expulsion capacity was increased in four patients after hysterectomy(29%). Conclusion: Our results demonstrate that hysterectomy does not cause a decrease in ano-rectal pressure and rectal sensitivity, but has an adverse effect on rectal expulsion capacity in a some of patients.

긴장성 요실금 환자에서 요도 과운동성과 내인성 괄약근 결함이 있는 환자의 증상과 특징의 비교 연구

선우재근 ( Jae Gun Sunwoo ),신영우 ( Young Woo Shin ) 대한산부인과학회 2007 Obstetrics & Gynecology Science Vol.50 No.12

목적: 긴장성 요실금 환자에서 서로 다른 4개의 진단기준으로 나눈 진단 그룹에 대하여 각각의 환자군의 임상적 증상과 특징을 비교하였다. 연구방법: 긴장성 요실금이 있는 여성 240명을 후향적으로 조사하였다. 환자들을 요역동학적 검사에 근거하여 크게 긴장성 요실금만 있는 군과 내인성 괄약근 결함이 있는 군으로 나누었다. 각 그룹의 환자들을 다시 요도의 과운동성이 있는 환자와 과운동성이 없는 환자로 구분하였다. 요로계 증상 (빈뇨, 급뇨, 배뇨 후 소변 떨어짐, 배뇨 곤란, 혈뇨, 재발성 감염)과 임상적 특징들을 모든 환자에서 조사하여 과운동성이 있는 환자와 비교하였다. 그룹 간 그리고 다양한 변수들은 적절한 통계적 방법을 이용하여 비교하였다. 결과: 110명 (45.8%)의 환자에서 과운동성만이 있었고, 115명 (47.9%)의 환자에서 과운동성과 내인성 괄약근 결함이 같이 있었다. 긴장성 요실금의 주요한 6가지 증상이 있는 네 그룹 사이의 현저한 통계학적 차이는 없으며, 당뇨가 있는 군과 이전에 요실금 교정 수술을 시행 받은 군에서는 통계학적으로 유의한 차이를 보였다. 과운동성이 없는 내인성 괄약근 결함 환자에서 당뇨의 유병률이 더 높은 경향을 보였다 (p=0.007). 과운동성이 없는 내인성 괄약근 결함 환자에서 이전에 요실금 교정 수술을 받은 경우가 더 많았다 (p=0.046). 또한 과운동성이 없는 내인성 괄약근 결함 환자군이 네 그룹 중에 나이의 평균과 중앙값이 가장 컸던 것 이외에는 측정 요인 간에 현저한 통계적 차이는 없었다. 결론: 증상만을 이용하여 긴장성 요실금의 심한 정도를 분류할 수는 없었으며, 긴장성 요실금 환자에서 치료의 선택, 특히 수술을 고려할 때는 요역동학적 검사를 포함하여 더 심도있는 검사를 해야하며 이에 대한 추가적인 임상적 기준이 연구 되어져야 할 것이다. Objective: To evaluate how patients in the four diagnosis groups differ in their symptoms and other characteristic levels in female stress urinary incontinence Method: A retrospective chart review of 240 patients with stress urinary incontinence was performed. Patients were categorized as intrinsic sphincter deficiency (ISD) or stress urinary incontinence (SUI). Within these groups, patients with hypermobility were separated from those without hypermobility. And then just patients with hypermobility were compared. Information on several types of symptoms and characteristics were captured for all patients. Results: There are nothing to do with among 4 each groups and 6 symptoms (frequency, urgency, postvoid dribbling, voiding difficulty, hematuria, recurrent infection) in stress urinary incontinence. Only in diabetic status and antiincontinence surgery did the diagnosis groups differ statistically. In diabetes, patient in the ISD without hypermobility tended to have a higher prevalence of diabetes than the other 3 groups (p=0.007). Patients in the ISD with hypermobility group were more likely to have antiincontinence surgery than thosein the SUI with hypermobility group (p=0.046). No other statistical comparisons were significant at the 0.05 level, although there is some evidence that patients in the ISD with hypermobility group were more likely to have a previous hysterectomy than those in the SUI with hypermobility group (p=0.072). Patients in the ISD without hypermobility group had the largest mean and median age among the four groups, and a statistically significant difference in age was detected across all groups (p<0.001). More specifically, the ISD without hypermobility group was significantly older than the SUI with hypermobility group. Conclusion: There was no statistically significant among 4 each group according to each 6 symptom in stress urinary incontinence. Of the variables, diabetes, antiincontinence surgery history, hormonal use, parity and age tended to have a statistically significant difference.

우측난소임신의 복강경적 치료 중 우연히 발견된 선천성 좌측 난소난관 형성부전 환자

장재연 ( Jae Yeon Jang ),김윤숙 ( Yun Sook Kim ),황인철 ( In Chul Hwang ),한효상 ( Hyo Sang Han ),선우재근 ( Jae Gun Sunwoo ),배동한 ( Dong Han Bae ) 대한산부인과학회 2009 Obstetrics & Gynecology Science Vol.52 No.11

Asymptomatic unilateral absence of a portion of a fallopian tube with or without adjacent ovarian agenesis is a very rare condition. The true incidence is unknown. Two etiologic causes are possible. Asymptomatic segmental torsion of the uterine tube and ovarian pedicle may occur for certain reasons during adulthood, in childhood, or even during the fetal stages. Consequently, torsion may give rise to necrosis and autoamputation. Alternatively, the absence of these organs may be congenital. Ovarian pregnancy is a rare form of ectopic pregnancy occurring in 0.5~1% of all ectopic pregnancies. Moreover, ovarian pregnancy in patient with congenital unilateral tubal and ovarian agenesis has not been reported. But, the presenting symptom is very similar and difficult to distinguish with other forms of ectopic pregnancy. Early detection is important role in preserving fertility to avoid the ablation of functional ovarian tissue. We report a case of congenital unilateral tubal and ovarian agenesis diagnosed during laparoscopic treatment of ovarian pregnancy.

항동해제에 따른 생쥐 동결수정란의 생존율및 체외발달율

차상헌,선우재근,박효숙,이임순,조태호,Cha, Sang-Hun,SunWoo, Jae-Gun,Park, Hyo-Suk,Lee, Im-Soon,Cho, Tai-Ho 대한생식의학회 1990 Clinical and Experimental Reproductive Medicine Vol.17 No.2

This study was carried out to clarify the effects of various kinds of cryoprotectants which were frequently used in freezing embryos of domestic animals on the survival of frozen-thawed mouse embryos. Mouse embryos were collected by hyperstimulation induction of ICR mouse. The samples were slowly cooled ($l^{\circ}C/min$) to temperatures between $-7^{\circ}C$ and $-30^{circ}C$ before direct transfer to liquid nitrogen ($-196^{\circ}C$) and thawed rapidly ($-500^{\circ}C$/min). As cryoprotectants, Glycerol, DMSO, Ethylene glycol and Propylene glycol were used and applied each 2 cell, 8 cell, morula in embryo stage. After normal mouse embryos developed to blastocyst by in vitro culture, we observed recovery rate and developing rate of embryos at thawing. The results obtained in these experiments were as follows : 1. The in vitro development rate from the frozen-thawed 2 cell embryos to the blastocyst were 67.7% in ethylene glycol, 65.7% in Propylene glycol, 55.2% in glycerol and 50.0% in DMSO respectively. 2. The in vitro development rate from the frozen-thawed 8 cell embryos to the blastocyst were 83.6% in DMSO, 75.7% in glycerol, 52.2% in propylene glycol respectively. 3. The in vitro development rate from the frozen-thawed morula to the blastocyst were 84.2% in glycerol, 80.0% in DMSO, 66.6% in propylene glycol and 55.2% in ethylene glycol respectively.

중년 여성의 식사섭취 상태와 갱년기 증상 관련 요인 분석

김순경(Soon Kyung Kim),선우재근(Jae Gun Sunwoo) 대한지역사회영양학회 2007 대한지역사회영양학회지 Vol.12 No.1

The purpose of this study was to investigate the dietary intakes in middle-aged women (pre and postmenopausal) and relation to climacteric symptoms. We conducted the anthropometric measurements, climacteric symptoms survey, and dietary intake to assess the nutrient intakes. Also, dietary quality indices were evaluated. The subjects, over 40 years old, were 168 middle aged women (pre = 74, postmenopausal = 94), mean age was 48.9 ± 3.6yr. The anthropometric measurements showed that 59% of subjects were overweight and obese. Climacteric symptoms that were answered yes in 50% plus in the subjects, were dry eyes, forgetfulness, difficult concentration, large joint pain (shoulder), fatique, backache, dry skin, joint pain (ankle and knee), dry mouth, dizziness, depression and lonesomeness, snore, morning stiffness, and hot flash. Some symptoms showed significant difference between pre and postmenopausal groups. The average energy intake was 1602.1 Kcal, which was 82% of the Korean EER. The subjects had lower vitamin A, riboflavin, folic acid, Fe, Zn and Ca intake than Korean RI. The lowest nutrient intake was Ca. The mean of food intake was 1294.2 g and MAR of diet quality indices was 0.83. In relation to dietary factors with climacteric symptoms, significant correlations have been found between total protein, animal protein, lipid, cholesterol and niacin intake and fatique, energy, carbohydrate, thiamin, vitamin B6, folic acid, vitamin C, Na, K and Fe intake and dry skin, Ca intake and difficult concentration. Our results indicate that dietary factors (food and nutrients intake, INQ, NAR, MAR) may effect the prevention and reduction of some climacteric symptoms in middle aged women. (Korean J Community Nutrition 12(1) : 25~39, 2007)

폐경기를 전 · 후한 중년 여성의 무기질영양상태와 갱년기증상

김순경(Kim Soon Kyung),선우재근(Sunwoo Jae Gun),이은주(Lee Eun Ju) 韓國營養學會 2006 Journal of Nutrition and Health Vol.39 No.2

산전에 Propylthiouracil를 사용한 Hyperthyroidism 환자에서 Multiple Syndactyly를 동반한 1례

양규준 ( Kyu Joon Yang ),선우재근 ( Jae Gun Sunwoo ),김민관 ( Min Kwan Kim ),배동한 ( Dong Han Bae ) 대한주산의학회 1994 Perinatology Vol.5 No.4

폐경기 여성들의 혈중 납 농도 수준과 이에 영향을 주는 관련요인 분석

원성윤 ( Seong Youn Won ),김남수 ( Nam Soo Kim ),이병국 ( Byung Kook Lee ),선우재근 ( Jae Gun Sunwoo ) 대한산부인과학회 2012 Obstetrics & Gynecology Science Vol.55 No.4

목적 폐경과 이와 관련된 유관변수들이 여성들의 혈중 납 농도에 어떠한 영향을 주는지를 규명하기 위하여 본 연구를 시도하였다. 연구방법 83명의 폐경 중이거나 폐경을 경험한 여성과 52명의 폐경 전 여성을 연구대상자로 선정하였다. 혈중 납량은 원자흡광광도계로 분석하였다. 난포자극호르몬(follicle-stimulating hormone, FSH)과 성선자극호르몬(lutenizing hormone, LH)을 측정하였으며, 이외에 혈색소, 혈구용적치, 체질량지수를 연구변수로 측정하였다. 또한 음주와 흡연에 관한 정보도 확보하였다. 결과 폐경기의 여성들의 혈중 납량은 평균(95% 신뢰구간)은 2.27 μg/dL (2.12-2.41 μg/dL)로서 폐경 전 여성들의 평균 1.89 μg/dL (1.76-2.02μg/dL)보다 통계적으로 유의하게 높았다. 그러나 폐경기 여성들을 다시 구분한 폐경 중과 폐경 후의 평균은 양자 간에 통계적으로 유의한 차이가 없었다. 연령, 체질량지수, 혈색소 및 흡연과 음주여부를 통제한 다중회귀분석에서 폐경 여부만이 대수 변환한 혈중 납량을 증가시키는 유일한 독립변수이었으며, FSH과 LH는 유의한 영향을 주지 못했다. 결론 본 연구결과 폐경기 한국여성들의 혈중 납량은 폐경 전 여성들보다 유의하게 높은 것이 확인되었다. Objective This study assessed blood lead in women in relation to menopause and its association with other related relevant factors to evaluate the impact of menopausal status on blood lead levels in women. Methods Eighty three menopausal women and 52 premenopausal women without known occupational lead exposure were included. Blood lead was measured by atomic absorption spectrophotometry. Follicle-stimulating hormone (FSH) and Lutenizing hormone (LH) were measured in addition to hemoglobin, hematocrit and body mass index as an study variables. Information on smoking and drinking status were also obtained. Results The mean (95% confidence intervals) level of blood lead in menopausal women was 2.27 μg/dL (2.12-2.41 μg/dL) which was signifi cantly higher than premenopausal women (1.89 μg/dL, 1.76-2.02 μg/dL), but there was no statistical difference of mean blood levels between perimenopausal and postmenopausal women. In a multivariate regression analysis after adjusting for age, body mass index hemoglobin, drinking and smoking status, only menopausal status was a signifi cant predictor of increases in log transformed blood lead without any signifi cant contribution of FSH and LH. Conclusion These results confi rmed that menopausal status was associated with signifi cant increase of blood lead levels in Korean women who were not occupationally exposed to lead.