재래닭의 근육 성장과 관련되는 cDNA Clone의 염기서열 및 특성

선상수,명규호,국길,김남오,Sun, S.S.,Myung, K.H.,Kuk, K.,Kim, N.O. 한국가금학회 2006 韓國家禽學會誌 Vol.33 No.4

본 연구는 재래닭의 성장 관련 유용 유전자를 검색하기 위하여, 재래닭에서 발현되는 유전자와 코니쉬에서 발현되는 유전자를 subtraction하여 cDNA library를 구축하고, 염기서열을 밝혀서 재래닭 특이 유용 유전자를 검증하고자 하였다. cDNA library에서 얻은 clone들의 염기서열을 분석하여 나타난 결과를 5개의 clone을 비교 분석하였다. Clone NDS-1(618nt)은 비록 타 종과의 상동성은 낮으나(10%) 해당 과정에서 중요한 역할을 담당하는 triosephosphate isomerase이며, Clone NDS-6(651nt)는 자에서 해당 과정에 관여하는 glyceraldehyde-3-phosphate dehydrogenase로 여겨진다. 그러나 3가지 유전자(clone NDS-2, NDS-10, NDS-12)는 다른 유전자들과 비교하여 5.0%내외의 낮은 상동성을 보이고 고등 동물과 거의 유사성이 없으므로 재래닭 특이 성장 관련 유용 유전자일 가능성이 있다. This experiment was conducted to examine the effective DNA related with muscle growth of Korean native chicken. cDNA library was constructed with mRNA subtraction from Korean native chicken to Cornish. Total mRNA was purified from pectoralis muscle of adult chicken. Five clones were compared their DNA sequence and characteristics based on GenBank. Clone NDS-1 (618nt) was low homology (10%) with other species, but it is closely related with triosephosphate isomerase which is play an important role in glycolysis. Clone NDS-6 (651nt) is corresponding to glyceraldehyde-3-phosphate dehydrogenase. These two clones are encoding to enzymes in key role in glycolysis. However, other three clones (NDS-2, NDS-10, NDS-12) have low homology with other species about 5.0%. These clones were not similar with any other eukaryotics. Therefore, three clones (NDS-2, NDS-10, NDS-12) are high possibility of specific DNA for muscle growth in Korean native chicken.

INSULIN - LIKE GROWTH FACTOR - Ⅰ 의 가축체내 작용기전

선상수 ( S . S . Sun ) 한국영양사료학회 1992 韓國營養飼料學會誌 Vol.16 No.2

땅콩짚의 NaOH 처리에 의한 사료가치 개선에 관한 연구

명규호,김용식,선상수 ( K . H . Myung,Y . S . Kim,S . S . Sun ) 한국축산학회 1986 한국축산학회지 Vol.28 No.2

To evaluate the effect of NaOH treatment on peanut straw of the different parts, chemical composition, in vitro dry matter digestibility (IVDMD, %) and in vitro organic matter digestibility (IVOMD, %) were tested. The parts of peanut straw were devided into four parts of blade, stem, hull and whole straw. The results obtained are summarized as follows; 1. The blade contained more crude protein, crude fat, nitrogen free extract and crude ash, but reverse phenomina were observed in the contents of crude fiber and cell wall constituents than the others. 2. In vitro dry matter digestibilities (IVDMD, %) of the four parts of NaOH-treated peanut straw were highly significantly different (p$lt;0.01) in different levels of NaOH treatment and of in vitro times. 3. In vitro organic matter digestibilities (IVOMD, %) of the four parts of peanut straw were showed the same tendency with the IVDMD`s (%).

서방형 Bovine Somatotropin 투여에 의한 젖소의 홀몬 , 대사물질 및 장관 운동성의 변화

권동석(D . S . Kwon),김용식(Y . S . Kim),명규호(K . H . Myung),선상수(S . S . Sun) 한국축산학회 1991 한국축산학회지 Vol.33 No.9

The present study was conducted to evaluate the improvement of dairy performance of SR-BST (750 ㎎/head/14 d) using 6 lactating Holstein cows for 42 days. 3.5% FCM was significantly (P$lt;0.05) increased with SR-BST treatment while DM intake and feed efficiency were not greatly altered. Although glucose concentration in serum was not changed with treatment, NEFA concentration was increased twofold and uremia was found in BST cows. Serum concentration of BST was maintained higher by 7 days while that f IGF-I was kept higher ?-ing entire experimental period with BST treatment. However., no difference was found in insuim concentration. There was no difference in nutrient digestibility and gastro-intestial motility between treatments Although there was slightly higher percentage of milk fat, no great difference was found in milk composition with BST treatment. Therefore, it could be concluded from this study that SR-BST improved dairy performance through homeorhetic effect (nutrient repartitioning toward mammary gland) without altering nutrient digestibility and gastro-intestinal motility.

메추리의 6개 칼슘공급원에 따른 진정이용칼슘과 외견상이용칼슘가의 비교

김용식,선상수,명규호 ( Y . S . Kim,S . S . Sun,K . H . Myung ) 한국축산학회 1985 한국축산학회지 Vol.27 No.5

True available calcium(TAC) values of 6 calcium supplements were a little greater than apparent available calcium(AAC) value of them, however, no significant difference was found between two kinds of available calcium values. There were significant differences (p$lt;0.01) among each TAC and AAC values of 6 calcium supplements. TAC values of calcium carbonate and oyster shell in mixed diets were significantly (p$lt;0.05) greater then AAC values of them. TAC and AAC values of calcium carbonate were the highest as 99.94%±2.10 and 98.45%±1.07, and those of oyster shell were the lowest as 67.78%±1.96 and 67.40%±2.07 respectively.

칼슘급여수준· 급여방법·입자도·연령이 메추리의 진정이용칼슘가에 미치는 영향

김용식,선상수,명규호 ( Y . S . Kim,S . S . Sun,K . H . Myung ) 한국축산학회 1985 한국축산학회지 Vol.27 No.6

The relationships between calcium input level and excreta calcium, and true available calcium (TAC) values were Y=3.21 × 10^(-4)x²+ 0.52X-0.48 (r=0.9832^(**) and Y=-6.21 × 10^(-3)X² -0.03 (r=0.9738^(**)) respectively. The TAC values of limestone and oyster shell were the highest in the 40-80 mesh as 74.67%, and the lowest in the 10-30 mesh as 68.2% and 61.01% respectively. Excreted calcium from fed birds were significantly increased (p$lt;0.05) as the age of birds increased, however, excreted calcium from unfed birds were almost same in all the age of birds. The TAC value of calcium carbonate was the highest in that of 16 weeks old bird as 99.42±1.32%. The relationships between age of bird and excreta calcium, and TAC value were Y=0.578x-0.0475 (r=0.9960^(**)) and Y=-0.129X+100.0425 (r=-0.9850^(**)) respectively.

진정이용 ( 眞正利用 ) 광물질 측정방법에 의한 칼슘이용성 측정에 관한 연구 1 . 산란 메추리의 칼슘요구량 측정

명규호,선상수,김용식 ( K . H . Myung,S . S . Sun,Y . S . Kim ) 한국축산학회 1983 한국축산학회지 Vol.25 No.6

This experiment was conducted to determine the calcium requirement in the determination of calcium availability. Forty breeding Japanese quails of 13 weeks of age were randomly alloted to 10 calcium input levels(0.240,400,480,560,640,720,800,1000 and 1200㎎) with four replicates of one bird each. After the calcium fasting period of 24hr, the calcium unfed group (Omg) was fed the experimental diet to determine the metabolic fecal calcium (FmCa) plus endogeneous urinary calcium (UeCa), however, the calcium fed groups were forcedly fed the known amounts of calcium into the crop and fed the experimental diet afterward for 48hours. The results of the present study were summarized as follows. 1. According to the results of performance during experimental period, body weight, calcium devoid diet intake and excreta weight of tested birds were not significantly affected by the calcium input level. 2. The relationship between calcium excreta and input of 0 to 400㎎ was not showed, however, the highly correlation (P$lt;0.01) was found between calcium excreta and input of 480 to 1200㎎. The correlation equation between calcium excreta and input of 480 to 1200㎎ was Y = 0.9311 X - 463.6012 (r=0.9929**). 3. The maintenance requirement(FmCa+UeCa) of calcium was 0.71㎎ and production plus repletion requirement of that was 497.9 ㎎ for breeding quails. Therefore, the total calcium requirement of a breeding quail was 498.6㎎ in the determination of calcium availability. In conclusion, it could be said that a 72hours experimental period did not affect to body weight, calcium devoided diet intake and excreta weight of tested birds, and that the total requirement was 498.6㎎ in the determination of calcium availability.

알칼리장석-일라이트가 육용오리의 생산성 및 육질에 미치는 영향

국길,김정은,정진형,김재필,선상수,김광현,정완태,정광화,안종남,이병석,정일병,양철주,양재은,Kook K.,Kim J. E.,Jeong J. H.,Kim J. P.,Sun S. S.,Kim K. H.,Jeong Y. T.,Jeong K. H.,Ahn J. N.,Lee B. S.,Jeong I. B.,Yang C. J.,Yang J. E. 한국가금학회 2005 韓國家禽學會誌 Vol.32 No.4

본 연구는 3주령의 육용오린 사료에 알칼리장석-일라이트를 0, 0+ 항생제, 0.5, 1.0 및 $1.5\%$ 첨가한 5처리구에 3반복으로 각각 12수씩 배치하여 43일간 급여하여 생산성 및 육질에 미치는 영향을 알아보고자 실행하였다. 육성오리의 일당 증체량은 알칼리장석-일라이트 1.0와 $1.5\%$ 첨가구에서 약간 증가하였다(p>0.05). 사료섭취 량은 알칼리장석-일라이트 첨가구에서 증가하는 경향이었다(p>0.05). 혈중 글루코스 농도는 알칼리장석-일라이트 $0.5\%$ 처리구에서 약간 감소한 반면에(p>0.05) 혈중 요소태 질소 함량은 알칼리장석-일라이트 $0.5\%$ 첨가구에서 유의적으로 증가하였다(p<0.05). 콜레스테롤 함량은 알칼리장석-일라이트 $0.5\%$ 첨가구에서 유의적으로 감소하였다(p<0.05). 도체중과 도체율은 알칼리장석-일라이트 첨가수준에 따라 증가하는 경향이었다(p>0.05). 알칼리 장석-일라이트 급여에 의한 육용오리 가슴육의 조지방 함량은 알칼리장석-일라이트 $1.5\%$ 첨가구에서는 유의적으로 감소하였다(p<0.05). 육색의 명도와 황색도는 알칼리장석-일라이트에서 높게 나타났으며(p>0.05), 콜레스테롤 함량은 알칼리장석-일라이트 첨가구에서 감소하였다(p>0.05). 지방산 패도는 알칼리장석-일라이트 첨가구에서 약간 감소하였다(p>0.05). 알칼리장석-일라이트 첨가에 의한 포화지방산 비율이 약간감소하는 경향인 반면에 불포화지방산 비율이 약간 증가하는 경향을 나타내었으나 유의적인 차이는 없었다(p>0.05). 알칼리장석-일라이트 첨가에 의한 육성오리 간의 중금속 함량은 납 축적량이 비교적 높게 나타났다(p>0.05). 관능 평가(appearance)에서 알칼리장석-일라이트 1.0와 $1.5\%$ 첨가구에서 외관의 유의적인 개선 효과를 나타내었다 (p<0.05). 이상의 결과를 종합해 볼 때 육성오리에 대한 알칼리장석-일라이트 급여는 증체량의 개선효과와 더불어 가슴육의 조지방 함량의 감소 그리고 관능평가에서 외관의 개선 효과가 있음을 알 수 있었다. This experiment was conducted to investigate the effect of the supplemental alkali feldspar-ilite(feldspar) on growth performance and meat quality in broiler ducks for 43 days. One hundred eighty broiler ducks were divided into 5 groups of 12ducks. Dietary levels of feldspar 0, 0+antibiotics, 0.5, 1.0 and $1.5\%$ were added to experimental diets of each of the groups. Daily weight gain was slightly increased in 1.0 and $1.5\%$ feldspar treatments. Feed intake was slightly increased at all feldspar treatments. Glucose concentration of serum profile was decreased whereas BUN concentration was significantly increased (p<0.05) at $0.5\%$ feldspar. Cholesterol concentration was decreased at all feldspar treatments, this difference was especially observed in supplemental levels of $0.5\%$ feldspar(p<0.05). Carcass weight was increased at all feldspar treatments. Moisture and crude fat contents of proximate chemical composition in duck meat were decreased at all feldspar treatment, this difference especially was observed in supplemental levels of $1.5\%$ feldspar(p<0.05) on crude fat content. Lightness and yellowness was increased at all feldspar treatment. Cholesterol contents and TBA in meat were decreased, but this parameters were not difference by feldspar treatment. The composition of saturated fatty acids(SFA) was decreased, whereas unsaturated fatty acids(USFA) was slightly increased by feldspar treatment. The Pb content of heavy metal concentrations was increased with compared control, but not difference. The appearance of sensory evaluation was improved by supplemental feldspar, especially in supplemental feldspar, 1.0 and $1.5\%$(p<0.05). The results of this study indicate that the supplemental alkali feldspar may improve the production and meat quality of broiler ducks.

잉여양파의 첨가급여가 오리의 성장과 도체특성에 미치는 영향

안병진,장기,김성호,조남철,국길,최봉환,선상수,Ahn, B.J.,Jang, K.,Kim, S.O.,Cho, N.C.,Kook, G.,Choi, B.H.,Sun, S.S. 한국가금학회 2001 韓國家禽學會誌 Vol.28 No.3

본 연구의 목적은 상품화하기 어려워 버려지는 양파를 오리의 사료자원으로 이용하여 그 효과를 평가하고자 수행하였다. 시험동물은 1 일령 Cherry Valley F$_1$ 360수를 오리가 생체중 3 kg에 도달하는 시기인 약 7 주령 (49 일령)까지 사육하였다. 처리는 대조구, 세절 양파 3%, 6%, 양파 엑기스 5%, 10%, 착즙박 6%, 발효사료 3%, 6%, 등 8 처리로, 처리구 당 45수를 임의 배치하였다. 사료 섭취량은 대조구가 처리구에 비해 유의성(P<0.05)있게 높았으나, 증체량은 오히려 세절 양파 3%처리구가 가장 높았다. 사료 요구율에 있어서도 대조구와 비교해 발효사료 6%를 제외하고는 전체 처리구에서 낮게 나타나 사료효율도 우수한 경향을 보여주었다. 도체율은 양파 추출물 5% 처리구가 가장 높은 경향을 보였으나, 대조구와 처리구간에 유의성은 없었다. 복강내 지방의 중량은 대조구와 발효사료 6% 처리구에서 다른 처리구에 비해 높게 나타났으며, 세절 양파 6% 처리구에서 가장 낮은 복강 지방량을 보였다. 복강 지방율에 있어서는 발효사료 6% 처리구를 제외하고는 대조구와 각 처리구간에 유의적 (P<0.05)인 차이를 보였다. 콜레스테롤 함량은 대조구에 비하여 모든 처리구에서 약간 낮은 경향이었지만 유의적인 변화는 보이지 않았다. 양파급여 오리육에서 stearic acid ($C_{18:0}$)는 대조구에 비하여 현저히 낮아졌다 (P<0.05). 특히 세절 양파 3% 급여구와 양파 추출물 5% 급여구에서 유의적으로 줄어들었다. 반면에 arachidonic acid ($C_{20:4}$)는 대조구에 비하여 처리구에서 높게 측정되었다. 결과를 종합해 볼 때 잉여양파를 함유한 오리사료는 기호성, 사료 섭취량, 및 증체량에는 별다른 영향을 미치지 않았고 기호성도 다른 가금류에 비해 잡식성인 오리에게는 문제가 없는 것으로 나타나, 오리의 사료로서 충분한 가치가 있다고 사료된다. The objectives or this study were to examine reed value or waste onion in duck. Experimental chicks (Cherry Valley F$_1$, 1day old, 350 chicks) were randomly assigned in 8 treatment groups, each 45 chicks, and man-aged for 7 weeks (3 kg BW, 49 days old). Treatments were control, 3%, 6% of chopped onion-fed, 5%, 10% of onion extract fed, 6% of onion meal, 3%, 6% of fermented onion-fed. Feed intake was significantly (P<0.05) high in control group, but ADG was high in 3% chopped onion-fed group. Feed requirement was very efficient in all treatment groups except 6% fermented onion-fed group. Dressing rate was high in 5% onion extract-fed group, but it was not significant. Abdominal fat was the highest in control and 6% fermented onion-fed group and lowest in 6% chopped onion-fed group. Cholesterol content was lower in all treatment groups than in control group, but it was not significantly different. Stearic acid ($C_{18:0}$) content was significantly low in all treatment group (P<0.05). However, arachidonic acid ($C_{20:4}$) content was higher in treatment group than in control group. In result, onion diet was not significantly effect on palatability, feed intake, ADG in ducks. Duck meat of onion-fed contained low cho-lesterol and high unsaturated fat content.

BOVINE SOMATOTROPIN 투여에 의한 젖소의 유생산성 예측

명규호(K . H . Myung),문승주(S . J . Moon),선상수(S . S . Sun) 한국영양사료학회 1991 韓國營養飼料學會誌 Vol.15 No.5