우리나라 벼 근권으로부터 분리한 Azospirillum 균주의 미생물학적 특성

김원곤,서현창,김종평,김창진,이계호,유익동,Kim, Won-Gon,Seo, Hyun-Chang,Kim, Jong-Pyung,Kim, Chang-Jin,Lee, Ke-Ho,Yoo, Ick-Dong 한국미생물학회 1994 미생물학회지 Vol.32 No.2

경기도, 충청남도 일원의 벼 근권에서 400~900nmol $C_2H_4/hr/vial$의 아세틸린 환원 활성을 갖는 질소고정균인 Azospirillum 15균주를 분리하였다. 이 분리균주들은 $1.0{\times}3.0{\mu}m$크기를 갖는 vibrioid 형태이었으며 액체배양시 monopolar single 편모를 가졌다. 이 분리균주들은 생리적, 형태적 특성에 따라 두 그룹으로 분류되었는데, 그룹 I 균주들은 탄소원으로 glucose를 잘 이용하였고 biotin 요구성인 반면, 그룹 II 균주들은 glucose를 전혀 이용하지 못하였고 biotin 비요구성이었다. 또한 semisolid 무질소 배지에서 48시간 배양할 때 그룹 I 균주들은 원래의 vibrioid 형태에서 좀 더 길어지면서 S자 모양으로 변하는 pleomorphic 특성을 보인 반면, 그룹 II 균주들은 원래의 모양과 운동성을 유지하였다. 이상의 결과로부터 그룹 I 균주들은 Azospirillum lipoferum으로, 그룹 II 균주들은 Azospirillum brasilense으로 동정하였다. Fifteen strains of the nitrogen fixer Axospirillum were isolated from the rhizosphere of rice collected from Kyonggi-do and Chungcheongnam in Korea. They had strong acetylene-reducing activity of 400 of 900 nmol $C_2H_4$ per hour vial had a similar morphology in succinate-malate medium: vibrioid cells having a diameter of 1.0 ${\mu}m$ and a monopolar single flagellum in liquid media. According to their physiological and morphological characteristics, they were divided into two distinct groups, group I and group II. Group I strain were, unlike group II, distinguished by their ability to use glucose as a sole carbon source in nitrogen-free medium, requirement for biotin, and formation of wider, longer, and S-shaped cells in semisolid nitrogen-free malate medium. On the basis of their characteristics, strains belonging to group I were identified as Azospirillum lipoferum, while those belonging to group II were identified as Azospirillum brasilense.

충북 지역 학부모의 친환경 농산물에 대한 인식이 친환경 농산물 이용에 미치는 영향

최미경 ( Mi Kyung Choi ),서현창 ( Hyun Chang Seo ),백승희 ( Seung Hee Baek ) 한국식품영양학회 2010 韓國食品營養學會誌 Vol.23 No.2

The current exploratory study attempted to investigate the influence of environment-friendly agricultural products(EAPs) perception on EAPs consumption behavior. The survey was conducted to parents of elementary school in Chung-buk area by the help of h o m teachers of each school. Out of 500 questionnaires administered, a total of 305 completed questionnaires were returned, yielding a response rate of 61%. Regarding buying behavior, 89.6% of respondents have ever bought EAPs, among them, 52.9% usually bought in a mart. The reason to buy EAPs was mostly safety(57.6%), nutrition & health(30.4%), and quality(7.0%). The 11 item representing the attitude and knowledge were analyzed, resulting in three distinct dimensions-`attitude`, `knowledge`, and `concern`. The knowledge level of EAPs was slightly higher than medium. Concerning the influence of EAPs perception on EAPs consumption behavior, the more having high knowledge and attitude level on EAPs, the more lead to the EAPs consumption behavior. This implicit the importance of education to enhance the knowledge level and positive attitude towards EAPs.

경기 도시 지역 보건소 성인 방문자의 식생활과 건강 관련 라이프스타일 조사

권종숙(Jong Sook Kwon),김경민(Kyung Min Kim),서현창(Hyun Chang Seo),이윤나(Yoon Na Lee),임승건(Seung Geon Lim),최영숙(Young Sug Choi) 대한지역사회영양학회 2013 대한지역사회영양학회지 Vol.18 No.6

The purpose of this study was to investigate dietary behaviors and health-related lifestyles of adult visitors at a public health center in Gyeonggi urban area. A survey using questionnaire was conducted with 949 visitors at Seongnam public health centers from June to August, 2012. The data from 905 respondents were analyzed by gender, consisting of 322 males and 583 females, and age group, consisting of 243 low-age group (LA), 312 middle-age group (MA), 350 high-age group (HA), aged 20 to 30 years, 31 to 50 years, and 51 to 69 years, respectively. Average Body Mass Index was 23.0, which increased with age, and education level was high in LA. 59.0 percent of the subjects had various diseases, and the incidence of hypertension was the highest, followed by allergy, hyperlipidemia, diabetes mellitus, joint rheumatism. Incidence rates of chronic disease increased with age, which were lower than those from 2011 Korea National Health and Nutrition Examination Survey (KNHANES). Weekly drinking frequency rate and smoking rate decreased with age, and exercise performing rate was high at male and HA, which showed the same tendency as KNHANES. Female and HA showed more healthy dietary behaviors such as restricting salt, sugar, oily foods, foods containing food additives, calorie, caring for balanced diet, and referring to nutrition label. Subjects chose stress as the first factor, followed by diet, exercise, etc., among 13 suggested factors which strongly influence on human``s life-span. In general, public health center visitors, especially female and HA, showed better dietary behaviors and health-related lifestyles compared with KNHANES.

Nif A의 Overproduction에 의한 Klebsiella oxytoca 균주의 질소 고정력 증진 효과

서현창,유익동 한국식품영양학회 1992 韓國食品營養學會誌 Vol.5 No.3

논토양 중에 벼의 근권에 서식하여 벼에 협력적인 관계로 질소를 공급해 주는 질소고정 미생물인 Klebsiella oxytoca를 미생물 비료로 활용하기 위한 새로운 질소고정 균주의 육종을 시도하여 다음과 같은 결과를 얻었다. 1. Klebsiella oxytoca NG13, Klebsiella oxytoca SH31, Klebsiella oxytoca SH161에 pMC71A를 형질전환 한 경우, 무질소 배지에서 원래의 균주의 질소고정력에 비해 질소고정력이 각각 6.4배, 17.2배, 13.5배 증진되었다. 2. 15mM의 NH_4^+ 존재하에서, Klebsiella oxytoca NG13, Klebsiella oxytoca SH31, Klebsiella oxytoca SH161의 경우는 모두 질소고정능의 완전한 저해를 받은 반면 pMC71A가 도입된 Klebsiella oxytoca NG13 /pMC71A, Klebsiella oxytoca SH31 /pMC71A, Klebsiella oxytoca SH161/pMC71A,의 경우는 무질소 배지에서의 질소고정력에 비해 각각 13.7%, 7.7%, 6.2%의 질소고정 활성을 보였다. As a research for developing biofertilizers, Klebsiella oxytoca, an associative nitrogen fixer in the rhizosphere of rice plant in the soil of paddy field, was subjected to molecular breeding. The results obtained were as followings ; 1). By transforming pMC71A, Nif A overproducing plasmid, into Klebsiella oxytoca NG13, Klebsiella oxytoca SH31, and Klebsiella oxytoca SH161, nitrogenase activities in the absence of nitrogen source in the medium were increased 6.4, 17.2, and 13.5 times, respectively, in comparison with the parent strains. 2). Nitrogenase activity of Klebsiella oxytoca NG13, Klebsiella oxytoca SH31, and Klebsiella oxytoca SH161 was completely repressed in the presence of 15mM NH_4^+. But, nitrogenase activities of Klebsiella oxytoca NG13 /pMC71A, Klebsiella oxytoca SH31 /pMC71A, and Klebsiella oxytoca SH161/pMC71A, harboring pMC71A, were 13.7%, 7.7%, and 6.2% of the nitrogenase activities in the absence of nitrogen source in the medium, respectively.

한국 논에서 분리한 Azospirillum 속 균주의 Megaplasmid 분리와 Rhizobium meliloti nod ABC 유전자와의 상동성

서현창,유익동 한국식품영양학회 1992 韓國食品營養學會誌 Vol.5 No.1

우리 나라 논에서 분리된 Azospirillum 균주로부터 메가플라스미드를 확인한 결과 Azospirillum lipoferum AS192로부터 5개의 메가플라스미드를 확인했다. Azospirillum lipoferum AS192와 Azospirillum brasilense AS112의 메가플라스미드가 Rhizobium meliloti의 nod ABC와 유사성을 갖고 있다는 사실을 알았으며, 이 결과로부터 토양 미생물과 식물체 간의 관계의 초기 과정에 공통적인 기작이 존재한다고 추정할 수 있었다. Megaplasmids of Azospirillum strains isolated in the Korean paddy field were identified. Five megaplasmids were identified from Azospirillum lipoferum AS192. Homology between nod ABC gene of Rhizobium meliloti and megaplasmids of Azospirillum lipoferum AS192 and Azospirillum brasilense AS112 was found. This observation might have reflected a common mechanism in the early process of soil bacterial association with plants.

Effect of Dual Inoculation with Glomus mosseae and Bradyrhizobium sp. R938 on the Nitrogenase Activity of Arachis hypogeae L.

Seo, Hyun Chang,Kim, Chang Jin,Kim, Shin Duk,Yoo, Ick Dong 한국산업미생물학회 1991 한국미생물·생명공학회지 Vol.19 No.6

Bradyrhizobium sp. R938과 VA 내생 균근균인 Glomus mosseae의 동시 접종이 영호 땅콩의 질소고정력에 미치는 영향을 포트 실험을 통하여 조사하였다. 땅콩에 Glomus mosseae와 Bradyrhizobium을 동시 접종한 처리에서 질소고정력이 Bradyrhizobium만을 접종한 것보다 1.5배 증가되는 결과를 얻었다. 땅콩을 69일 동안 생육시킨 결과 근류의 크기도 내생균근균의 동시 접종으로 인해 증가되었다. 동시 접종 처리구에서 근류 하나의 평균 중량은 6.7㎎으로, Bradyrhizobium만을 접종한 것보다 1.5배 증가되는 결과를 보였다. 이상의 결과로부터 Bradyrhizobium과 Glomus mosseae의 동시 접종은 양분 흡수의 증진을 통한 질소고정력 증진효과 이외에도 VA 내생균근균으로 인한 호르몬 효과가 있으리라는 점을 추정할 수 있었다. The effect of dual inoculation with vesicular-arbuscular(VA) mycorrhizal fungi, Glomus mosseae, and Bradyrhizobium sp. R938 on the nitrogenase activity of peanut plant (Arachis hypogeae L. cv. Youngho) was studied in pot experiment. As a result of the dual inoculation with Glomus mosseae and Bradyrhizobium on peanut plant, nitrogenase activity was increased by 1.5 times compared with single inoculation with Bradyhizobium sp. R938. After 69 days growth of peanut plant, the size of nodules was also increased due to the mycorrhizal infection. The mean fresh weight per nodule was 6.7㎎ in treatment with the dual inoculation, which is the 1.5 times increase compared with single inoculation with Bradyrhizobium sp. R938. These results suggested that there might be a hormonal effect of VA mycorrhizal fungi in addition to the effect of increase in nitrogen fixing ability through the enhancement of nutrient absorption.

Structural Characteristics of Novel Branched Oligosaccharides Synthesized by a Maltose Acceptor Reaction with Dextransucrase from Leuconostoc mesenteroides M-12

Seo, Hyun Chang 한국식품영양학회 1997 韓國食品營養學會誌 Vol.10 No.1

효소반응과 부분 산가수분해 결과를 해석하여 Leuconostoc mesenteriodes M-12 덱스트란수크라제 억셉터 반응 산물이 새로운 분지올리고당의 구조를 확인하였다. 분지올리고당 B_4의 구조는6^2-O-α-D-kojibiosylmaltose인 것으로 확인되었으며, 분지올리고당 B_5의 구조는 6^3-O-α-D-kojibiosylpanose였다. 억셉터 반응산물을 덱스트라나제로 분해한 결과 새로운 올리고당인 D_4를 확인할 수 있었다. 억셉터 반응의 산물을 억셉터로 이용한 두 번째 억셉터 반응의 생성물을 덱스트라나제 처리하여 D_4를 얻었는데 덱스트라나제와 글루코아밀라제에 의해 분해되지 않았다. 그 구조는 6^2-O-α-D-kojibiosylisomaltose로 확인되었다. 직선상 또는 분지결합을 가진 d.p. 6이하의 억셉터 반응산물의 생성 패턴도 확인하였다. The structures of novel branched oligosaccharidse synthesized by the acceptor reaction with dextransucrase from Leuconostoc mesenteriodes M-12 were proposed in accordance with the results obtained from enzymatic hydrolysis and a partial acid hydrolysis. The structure of branched oligosaccharide B_4 was shown to be 6^2-O-α-D-kojibiosylmaltose. Branched oligosaccharide B_5 was shown to be 6^3-O-α-D-kojibiosylpanose. By reacting the acceptor reaction products with endodextranase a novel branched oligosaccharide(D_4) coule be produced. D_4 was derived from the result of endodextranase hydrolysis of oligosaccharides synthesized by the second acceptor reaction with dextransucrase and was resistant to endodextranase and glucoamylase. The proposed structure of D_4 was 6^2-O-α-D-kojibiosylisomaltose. Formation pattern of the acceptor reaction products smaller than d.p. 6 with linear or branched linkage was also shown.

Glomus mosseae와 Rhizobium meliloti의 동시접종이 알팔파의 생장 및 질소고정활성에 미치는 영향

유익동,서현창,김원곤,박동진,조갑연,김창진 한국산업미생물학회 1993 한국미생물·생명공학회지 Vol.21 No.2

Rhizobium meliloti R445와 VA 내생 균근균인 Glomus mosseae의 동시접종이 알팔파의 생장, 근류형성, 질소고정력에 미치는 효과를 포트 실험을 통해 조사하였다. 63일 동안 생장시킨 후 알팔파의 경장, 잎의 수, 잎의 크기가 Glomus mosseae와 Rhizobium meliloti를 동시 접종한 결과 증가되었다. 한편, Rhizobium meliloti만을 접종한 것보다 1.4배의 건물중의 증가가 확인되었다. 근류의 수와 식물체당 근류의 평균중량도 내생균근균의 접종으로 인해 증가되었다. 동시 접종 처리구에서 식물체당 근류의 평균 중량은 60.3 밀리그램으로, Rhizobium meliloti만을 접종한 것보다 1.7배 증가되는 결과를 보였다. 한편, 알팔파에 Glomus mosseae와 Rhizobium meliloti를 동시 접종한 처리에서 질소고정력이 Rhizobium meliloti만을 접종한 것보다 1.5배 증가되는 결과를 얻었다. 이상의 결과로 볼 때 알팔파의 생육촉진 효과는 VA 내생 균근균의 감염을 통한 양분 흡수의 증가와 질소고정활성 증가의 결과에 기인한 것으로 생각되었다. Effect of dual inoculation with vesicular-arbuscular (VA) mycorrhizal fungi, Glomus mosseae, and Rhizobium meliloti R445 on growth, nodulation, and nitrogenase activity of alfalfa (Medicago sativa L. cv. Vernal) were examined in pot experiment. After 63 days growth, shoot length, number of leaf, and leaf size of alfalfa were increased as a result of dual inoculation with Glomus mosseae and Rhizobium meliloti. Total dry weight of alfalfa plant was increased 1.4 times compared to single inoculation with Rhizobium meliloti. Nodule number and mean fresh weight of nodule per plant were also increased due to the mycorrhizal infection. The mean fresh weight of nodule per plant was 60.3 ㎎ in treatment with the dual inoculation, which was comparable to the 1.7 times increase compared to single inoculation with Rhizobium meliloti. As a result of the dual inoculation with Glomus mosseae and Rhizobium meliloti on alfalfa, nitrogenase activity was increased by 1.5 times compared to single inoculation with Rhizobium meliloti. These results showed that the effect of growth stimulation on alfalfa was caused by the enhancement of nutrient absorption and increase in nitrogen fixing ability through the inoculation with VA mycorrhizal fungi.