미생물 생장 예측모델과 반응표면분석법을 이용한 Vibrio parahaemolyticus의 신속 증균배지 개발

서연희,이소영,김은지,오세욱,Yeon-Hee Seo,So-Young Lee,Unji Kim,Se-Wook Oh 한국식품위생안전성학회 2023 한국식품위생안전성학회지 Vol.38 No.6

In this study, we developed Rapid Enrichment Broth for Vibrio parahaemolyticus (REB-V), a broth capable enriching V. parahaemolyticus from 10⁰ CFU/mL to 10⁶ CFU/mL within 6 hours, which greatly facilitates the rapid detection of V. parahaemolyticus. Using a modified Gompertz model and response surface methodology, we optimized supplement sources to rapidly enrich V. parahaemolyticus. The addition of 0.003 g/10 mL of D-(+)-mannose, 0.002 g/10 mL of L-valine, and 0.002 g/10 mL of magnesium sulfate to 2% (w/v) NaCl BPW was the most effective combination of V. parahaemolyticus enrichment. Optimal V. parahaemolyticus culture conditions using REB-V were at pH 7.84 and 37℃. To confirm REB-V culture efficiency compared to 2% (w/v) NaCl BPW, we assessed the amount of enrichment achieved in 7 hours in each medium and extracted DNA samples from each culture every hour. Real-time PCR was performed using the extracted DNA to verify the applicability of this REB-V culture method to molecular diagnosis. V. parahaemolyticus was enriched to 5.452±0.151 Log CFU/mL in 2% (w/v) NaCl BPW in 7 hours, while in REB-V, it reached 7.831±0.323 Log CFU/mL. This confirmed that REB-V enriched V. parahaemolyticus to more than 10⁶ CFU/mL within 6 hours. The enrichment rate of REB-V was faster than that of 2% (w/v) NaCl BPW, and the amount of enrichment within the same time was greater than that of 2% (w/v) NaCl BPW, indicating that REB-V exhibits excellent enrichment efficiency. 본 논문은 적은 농도로 존재하는 Vibrio parahaemolyticus를 6시간이내에 10⁶ CFU/mL까지 신속하게 배양가능한 배지인 Rapid Enrichment Broth for V. parahaemolyticus(REB-V)를 개발하여 V. parahaemolyticus를 신속하게 검출할 수 있도록 하였다. Modified Gompertz model과 RSM를 활용하여 V. parahaemolyticus를 신속하게 증균할 수 있도록 첨가 성분을 최적화하였다. 그 결과 2%(w/v) NaCl BPW에 D-(+)-mannose 0.3 g/L, L-valine 0.2 g/L, magnesium sulfate 0.2 g/L를 첨가하였을 때 V. parahaemolyticus의 증균량이 최대였다. REB-V의 배양조건을 pH 7.84와 37℃으로 최적화하였으며, 2%(w/v) NaCl BPW와 비교하여 REB-V의 증균 효율을 확인하기 위해 7시간 증균을 통해 증균량을 평가하였다. 또한 매시간마다 배양액에서 DNA를 추출하였고, 추출한 DNA로 real-time PCR을 수행하여 REB-V의 분자진단법 적용가능성을 확인하였다. 그 결과 7시간 동안 2%(w/v) NaCl BPW에서 V. parahaemolyticus는 5.452±0.151 Log CFU/mL까지 증균되었고, REB-V에서는 7.831±0.323 Log CFU/mL의 V. parahaemolyticus가 증균되었다. 최종적으로 REB-V에서 6시간 이내에 10⁶ CFU/mL 이상으로 증균된 것을 확인하였고, REB-V의 증균속도가 2%(w/v) NaCl BPW보다 빠르고 같은 시간 내의 증균량이 2%(w/v) NaCl BPW보다 많았음을 통해 REB-V의 증균효율이 우수한 것으로 확인되었다.

체조지도자의 긍정심리자본과 경력몰입, 직무만족의 구조관계

서연희(Seo, Yeon-Hee) 한국체육과학회 2020 한국체육과학회지 Vol.29 No.1

This study was to explore the relationship among positive psychological capital, career commitment and job satisfaction of the gymnastic coaches. For this, the head coaches and coaches of gymnastic teams(elementary・middle・high school, university, semi-pro team) were surveyed. Except for none questionnaires, final 241 out of 250 ones was statistically managed and SPSS 23.0 and LISREL 8.80 were used for statistical analysis. The result of this research was as follows. First, the positive psychological capital of the gymnastics coaches in gymnastic teams are significant to career commitment of the head coaches and coaches of gymnastic teams. Second, the positive psychological capital of the gymnastics coaches in gymnastic teams are significant to job satisfaction of the head coaches and coaches of gymnastic teams. Third, the career commitment of the gymnastics coaches in gymnastic teams are significant to job satisfaction of the head coaches and coaches of gymnastic teams.

체조선수들의 성취목표성향과 자기관리가 심리기술에 미치는 영향

서연희(Yeon Hee Seo) 한국체육교육학회 2012 한국체육교육학회지 Vol.17 No.3

본 연구는 체조선수들의 성취목표성향과 자기관리가 심리기술에 어떠한 영향을 미치는지 관계를 분석하였다. 연구 대상은 문화체육부 장관기 및 대학, 일반 선수권대회에 참가한 체조선수 총 126명의 자료를 수집하였으며, 본 연구에 사용된 통계방법은 설문지의 타당도 및 신뢰도를 검증하기 위하여 탐색적 요인분석 및 신뢰도 분석을 하였다. 각 변수 간 관계를 알아보기 위한 상관관계분석과 중다 회귀분석을 시행하였다. 분석결과 첫째, 성취목표성향이 심리기술에 미치는 영향은 과제성향이 목표설정과 팀조화에 영향을 미쳤으며, 자기성향은 심상요인에 영향을 미치는 것으로 나타났다. 둘째, 자기관리(몸 관리와 훈련관리, 정신관리)와 심리기술에 미치는 영향은 몸 관리와 집중력, 자기관리의 하위요인 몸 관리와 훈련관리, 정신관리가 심리기술 하위요인인 목표설정에 상대적으로 높은 영향이 있는 것으로 나타났다. 또한 대인관계와 정신관리는 팀조화에 관계되며, 그리고 정신관리는 심상과 의지력, 훈련관리는 불안조절에 영향을 미치는 것으로 나타났다. This study analyzes what relationship goal orientation & self-management of gymnasts have with psychological skills. For this research, data of 126 gymnasts who participated in various competitions sponsored by Ministry of culture, sports & tourism and universities or gymnastics championship have been collected. Statistical methods used in this analysis are frequency analysis, exploratory factor analysis and reliability analysis for calculating validity and reliability. Correlation analysis and multiple regression analysis are conducted for identifying relationship between individual variables. As a result of analysis, first, when it comes to how tendency to achieve goals affects psychological skills, task-disposition affects setting goals and harmony of teams, and also self-disposition affects mental factors. Second, when it comes to how goal orientation affects self-management and psychological skills, body management, concentration, body management & training management as a subordinate concept of self-management and mental management relatively highly affect goal setting as a subordinate concept of psychological skills. It is also found that personal relations and mental management are related to harmony of teams, and mental management affects mental state and willpower and training management affects anxiety control.

국가대표 여자 체조선수들의 심리기술훈련 프로그램 적용에 따른 효과 검증

서연희(Yeon Hee Seo),박현조(Hyun Jo Park),강성구(Sung Goo Kang) 한국여성체육학회 2008 한국여성체육학회지 Vol.22 No.4

이단평행봉의 Ray 동작 시 숙련도에 따른 운동학적 차이

서연희(Yeon Hee Seo),이주형(Joo Hyung Lee),이석희(Suk Hee Lee) 한국여성체육학회 2011 한국여성체육학회지 Vol.25 No.3

The purpose of this study was to analyze kinematic factors of the Ray that is an uneven parallel bar skill in gymnastics. For this study, four national woman gymnasts participated in this experiment and they were divided into two groups (skilled and less skilled group) by means of performance scores. The two digital video cameras (Sony, PD-150) were used to record motions, and Kwon3D 3.1 was used to process data and analyze factors. The results were as follows: First, in down swing phase the width of swing for Skilled Group (SG) was greater than Less skilled group LG), that is, a vertical velocity in upward swing increased, and the Cog of SG was greater than LG at a release, that is, SG secured a maximum height at an air phase. Second, at an air phase and a landing, horizontal displacement of SG was less than LG, but vertical displacement of SG was greater than LG. This means that SG secured a maximum height with a body projecting, but LG landed at near a bar with body projecting ahead. Third, at release, SG represented a rapid vertical velocity and a large shoulder joint angle which was staple factors with a body rising upward made a body stable landing to compare with the LG, but LG did the opposite results. In conclusion, using rotation gained with a wide down swing at release, SG made extension of shoulder joint, a high vertical position and rapid velocity. Therefore increasing an air position made a good air rotation and stable landing.

2013년 평균대 규칙 개정 전 · 후 비교분석

서연희(Seo, Yeon-Hee),전혜령(Jeon, Hea-Ryeong) 한국체육과학회 2013 한국체육과학회지 Vol.22 No.5

Balance beam according to the configuration rules amended before 2009 and revised in 2013 comparative analysis routines, detailed strategies are as follows: Difficulty analyzing information before acting revision after revision of the rules applied to the evaluation of each difficulty level through the comparative analysis Difficulty Changes JW, GC, DL players were all the same. Values that connect the JW player +0.60 points before the revision of the contents was evaluated in the same acting in accordance with the rules and values that the revision of the 0.10 point difference was 0.50 points. The G. L player +0.40 points before revision after revision value from 0.30 to 0.10 points points points showed differences, D. L in that player before revision after revision 0.30 +0.50 points difference was 0.20 points. General Gam Gam Company and certain additional items, including the JW player was evaluated before revision after revision from 1.10 points to 1.70 and 0.60 point deduction this up, GC player before revision after revision 2.40 points from 1.90 points get deducted for deduction of 0.30 was added. DL players before revision after revision 2.60 points from 2.20 points to 0.40 points of difference was deducted. Balance beam configuration and the results of a comparative analysis artistry deduction JW player configuration and artistry deducted before revision, in the absence of additional items, including penalties received deduction of 0.30 points, GC player before revision after revision 0.30 points from 0.10 points received deduction for the difference was 0.20 points. DC players before revision after revision 0.30 points from 0.10 points get deducted for the difference was 0.20 points.

체조 국가대표 선수들의 계층구조분석(AHP)을 활용한 자기관리 검사지의 가충치 변화 탐색

서연희(Seo, Yeon-Hee) 한국체육과학회 2013 한국체육과학회지 Vol.22 No.3

The purpose of this study, twenty-eight Olympic Games, World Championships, Asian Games gymnastics medalists pairwise comparison (pairwise comparison) was performed. In this study, uniform, scoring, and specifically how to use a strip of self-management has been recognized in the field of sports athletes, researchers and field leaders to accurate measurement by utilizing AHP item self-administered test for the relative importance was quantified by calculating The results are as follows: Was assessed by Training Management (0.342) mental Management (.334),body care(0.256) (0.068), interpersonal management of the relative importance of factors for the self-administered Questionnaire top gymnast. Interpersonal management topic gymnast strip the self-management of the sub-factors of body care items and food before the fight ‘regulates’ the highest relative importance to maintain harmonious interpersonal relationships with colleagues ‘.’ It was rated with the highest relative importance. Was the highest relative importance to the topic training management training on a regular basis’, ‘believe in myself.’ Mental management topic was rated as the highest relative importance. AHP analysis, the expert group were self-administered test the relative importance of the items that give different. Reflect the needs of the sports venues and professionals as research suggests the possibility of utilizing the present study, considering the relative importance of the self-administered test tool gymnast. These studies to gain a foothold in the field to improve the performances of the players in various sports, including gymnastics, hope this helps.

기계체조 선수의 성취목표성향 자기관리 및 수행전략의 구조관계 분석

서연희(Seo, Yeon-Hee) 한국체육과학회 2014 한국체육과학회지 Vol.23 No.5

This research drew a purpose through the Analysis of The Structure Relation Among Achievement Goal Orientation, Self-Regulation, and Performance Strategy in Gymnast. First, it was statistically significant influence of structural relationships achievement goal orientation and self-management of the gymnast. Second, there was a statistically significant influence of the structural relationship between self-management and gymnast performing strategy. Third, a statistically significant influence perform structural relationship between achievement goal orientation and strategy of the gymnast. It can be seen that the effect to improve the performances of the various strategies philosophical psychological aspects of the nature of the sport exercises to strengthen and maintain individual player in the light these findings. Based on this study, it is suggested that more specific achievement goal orientation, according to age and gender sub-factors between self-management and the need to perform strategic analysis to see events as players age am low in subsequent studies.

체육전공 예비교사들의 교육실습 전 · 후에 나타난 교직관의 상대적 중요도 차이

서연희(Seo, Yeon-Hee) 한국사회체육학회 2017 한국사회체육학회지 Vol.0 No.68

This study is to analyze a difference in relative importance of teaching profession view shown before · after classroom teaching practice of pre-service teachers majoring in physical education. For this end, an AHP survey was conducted. with regard to AHP analysis, Methods: pre-service teachers at 7 colleges of education were selected according to region (1 college in Seoul, 2 in Chungnam, 1 in Chungbuk, 1 in Jeonnam and 2 in Jeonbuk). The results of analyzing pairwise comparison questionnaires for respective 164 pre-service teachers before · after student teaching are as follows. First, with regard to relative importance of teaching profession view before student teaching, it was shown that ‘positive teaching profession view (0.381)’ had the highest relative importance, being followed by ‘negative teaching profession view (0.381)’ and ‘social teaching profession view (0.218)’. And it was shown to be the lowest in ‘teaching profession aspiration (0.152)’. Second, it was shown that relative importance of teaching profession view shown after student teaching was the highest in ‘teaching profession aspiration (0.401)’, being followed by‘ positive teaching profession view (0.266)’ and ‘negative teaching profession view (0.184)’. And it was shown to be the lowest in ‘social teaching profession view (0.149)’. Third, with regard to a change in priority and relative importance of teaching profession view in pre-service teachers majoring in physical education before · after student teaching, positive teaching profession view factor weight was decreased by 0.115. And negative teaching profession view factor weight was decreased by 0.065. Social teaching profession view factor weight was decreased by 0.069. However, it was shown that teaching profession aspiration factor weight is increased by 0.249. the significance of hands-on experience through pre-service teachers’ teaching practice and the necessity of follow-up study to enhance pre-service teachers’ competency as teacher and promote professionalism was proposed.

초등학생의 체육수업 소외와 스트레스 관계

서연희(Yeon Hee Seo),이대형(Dae Hyung Lee),송지환(Ji Hwan Song) 한국체육교육학회 2011 한국체육교육학회지 Vol.16 No.1

본 연구는 초등학교 학생들이 경험한 체육수업소외와 체육수업스트레스의 변인간의 관계성을 알아보고, 체육수업 내실화에 기여하는데 목적이 있다. 본 연구를 위해 J시에 소재한 초등학생 6학년 아동 전체(남 127명, 여 153명)의 설문자료를 분석에 사용하였으며, 빈도분석(frequency analysis), 요인분석(factor analysis), 상관관계(correlation analysis), 중다회기분석(multiple regression analsis)의 통계적 검증을 통해 다음과 같은 결론을 도출하였다. 첫째, 초등학생들이 체육수업소외(체육교사소외, 운동능력소외, 교과내용소외)는 학습내용스트레스에 영향을 미치는 것으로 나타났다. 둘째, 체육수업소외(친구관계소외, 운동능력소외, 교과내용소외)는 친구들과의 마찰 스트레스에 영향을 미치는 것으로 나타났다. 셋째, 체육수업소외(운동능력소외, 친구관계소외)는 자신감부족 스트레스에 영향을 미치는 것으로 나타났다. 넷째, 체육수업소외(체육교사소외, 교과내용소외)는 교사불만 스트레스에 영향을 미치는 것으로 나타났다. 다섯째, 체육수업소외(운동능력소외, 체육교사소외, 친구관계소외, 교과내용소외)는 시험부담 스트레스에 영향을 미치는 것으로 통계적으로 유의한 결론이 나타났다. 이와 같은 결과는 초등학생들의 체육수업소외는 학습내용 스트레스, 친구들과 마찰 스트레스, 자신감부족 스트레스, 교사불만 스트레스, 시험부담 스트레스에 영향을 미치고 있음이 확인되었다. This study searches relation between fantast of physical education class estrangement and Cheyuksueopseuteureseu that primary school students recognize, and is tendencious to contribute at physical education class main room Tuesday. Used question data of J city locating elementary school pupil 6 class child whole (127 others, 153 women) in analysis for this study, and drew following conclusion through frequency assay, interrelation, Jungdahoegibunseok`s statistical verification. First, elementary school pupils appeared that physical education class estrangement (gymnast, motor ability, faith subject contents estrangement) affects to Hakseupnaeyongseuteureseu. Second, was expose that physical education class estrangement (friend relation, motor ability, subject contents) affects in attrition stress with friends. Third, was expose that physical education class estrangement (motor ability, friend relation) affects in pride tribe stress. Fourth, was expose that physical education class estrangement (gymnast estrangement, subject contents estrangement) affects in teacher complaint stress. Fifth, conclusion that keep in mind statistically that physical education class estrangement (motor ability, gymnast, friend relation, subject contents) affects in examination burden stress appeared.