미생물 생장 예측모델과 반응표면분석법을 이용한 Vibrio parahaemolyticus의 신속 증균배지 개발

서연희,이소영,김은지,오세욱,Yeon-Hee Seo,So-Young Lee,Unji Kim,Se-Wook Oh 한국식품위생안전성학회 2023 한국식품위생안전성학회지 Vol.38 No.6

In this study, we developed Rapid Enrichment Broth for Vibrio parahaemolyticus (REB-V), a broth capable enriching V. parahaemolyticus from 10⁰ CFU/mL to 10⁶ CFU/mL within 6 hours, which greatly facilitates the rapid detection of V. parahaemolyticus. Using a modified Gompertz model and response surface methodology, we optimized supplement sources to rapidly enrich V. parahaemolyticus. The addition of 0.003 g/10 mL of D-(+)-mannose, 0.002 g/10 mL of L-valine, and 0.002 g/10 mL of magnesium sulfate to 2% (w/v) NaCl BPW was the most effective combination of V. parahaemolyticus enrichment. Optimal V. parahaemolyticus culture conditions using REB-V were at pH 7.84 and 37℃. To confirm REB-V culture efficiency compared to 2% (w/v) NaCl BPW, we assessed the amount of enrichment achieved in 7 hours in each medium and extracted DNA samples from each culture every hour. Real-time PCR was performed using the extracted DNA to verify the applicability of this REB-V culture method to molecular diagnosis. V. parahaemolyticus was enriched to 5.452±0.151 Log CFU/mL in 2% (w/v) NaCl BPW in 7 hours, while in REB-V, it reached 7.831±0.323 Log CFU/mL. This confirmed that REB-V enriched V. parahaemolyticus to more than 10⁶ CFU/mL within 6 hours. The enrichment rate of REB-V was faster than that of 2% (w/v) NaCl BPW, and the amount of enrichment within the same time was greater than that of 2% (w/v) NaCl BPW, indicating that REB-V exhibits excellent enrichment efficiency. 본 논문은 적은 농도로 존재하는 Vibrio parahaemolyticus를 6시간이내에 10⁶ CFU/mL까지 신속하게 배양가능한 배지인 Rapid Enrichment Broth for V. parahaemolyticus(REB-V)를 개발하여 V. parahaemolyticus를 신속하게 검출할 수 있도록 하였다. Modified Gompertz model과 RSM를 활용하여 V. parahaemolyticus를 신속하게 증균할 수 있도록 첨가 성분을 최적화하였다. 그 결과 2%(w/v) NaCl BPW에 D-(+)-mannose 0.3 g/L, L-valine 0.2 g/L, magnesium sulfate 0.2 g/L를 첨가하였을 때 V. parahaemolyticus의 증균량이 최대였다. REB-V의 배양조건을 pH 7.84와 37℃으로 최적화하였으며, 2%(w/v) NaCl BPW와 비교하여 REB-V의 증균 효율을 확인하기 위해 7시간 증균을 통해 증균량을 평가하였다. 또한 매시간마다 배양액에서 DNA를 추출하였고, 추출한 DNA로 real-time PCR을 수행하여 REB-V의 분자진단법 적용가능성을 확인하였다. 그 결과 7시간 동안 2%(w/v) NaCl BPW에서 V. parahaemolyticus는 5.452±0.151 Log CFU/mL까지 증균되었고, REB-V에서는 7.831±0.323 Log CFU/mL의 V. parahaemolyticus가 증균되었다. 최종적으로 REB-V에서 6시간 이내에 10⁶ CFU/mL 이상으로 증균된 것을 확인하였고, REB-V의 증균속도가 2%(w/v) NaCl BPW보다 빠르고 같은 시간 내의 증균량이 2%(w/v) NaCl BPW보다 많았음을 통해 REB-V의 증균효율이 우수한 것으로 확인되었다.

한국어판 간호대학생의 인공지능에 대한 태도 측정도구 신뢰도 및 타당도 검증

서연희(Seo, Yon Hee),안정원(Ahn, Jung-Won) 한국간호교육학회 2022 한국간호교육학회지 Vol.28 No.4

체조선수들의 성취목표성향과 자기관리가 심리기술에 미치는 영향

서연희(Yeon Hee Seo) 한국체육교육학회 2012 한국체육교육학회지 Vol.17 No.3

본 연구는 체조선수들의 성취목표성향과 자기관리가 심리기술에 어떠한 영향을 미치는지 관계를 분석하였다. 연구 대상은 문화체육부 장관기 및 대학, 일반 선수권대회에 참가한 체조선수 총 126명의 자료를 수집하였으며, 본 연구에 사용된 통계방법은 설문지의 타당도 및 신뢰도를 검증하기 위하여 탐색적 요인분석 및 신뢰도 분석을 하였다. 각 변수 간 관계를 알아보기 위한 상관관계분석과 중다 회귀분석을 시행하였다. 분석결과 첫째, 성취목표성향이 심리기술에 미치는 영향은 과제성향이 목표설정과 팀조화에 영향을 미쳤으며, 자기성향은 심상요인에 영향을 미치는 것으로 나타났다. 둘째, 자기관리(몸 관리와 훈련관리, 정신관리)와 심리기술에 미치는 영향은 몸 관리와 집중력, 자기관리의 하위요인 몸 관리와 훈련관리, 정신관리가 심리기술 하위요인인 목표설정에 상대적으로 높은 영향이 있는 것으로 나타났다. 또한 대인관계와 정신관리는 팀조화에 관계되며, 그리고 정신관리는 심상과 의지력, 훈련관리는 불안조절에 영향을 미치는 것으로 나타났다. This study analyzes what relationship goal orientation & self-management of gymnasts have with psychological skills. For this research, data of 126 gymnasts who participated in various competitions sponsored by Ministry of culture, sports & tourism and universities or gymnastics championship have been collected. Statistical methods used in this analysis are frequency analysis, exploratory factor analysis and reliability analysis for calculating validity and reliability. Correlation analysis and multiple regression analysis are conducted for identifying relationship between individual variables. As a result of analysis, first, when it comes to how tendency to achieve goals affects psychological skills, task-disposition affects setting goals and harmony of teams, and also self-disposition affects mental factors. Second, when it comes to how goal orientation affects self-management and psychological skills, body management, concentration, body management & training management as a subordinate concept of self-management and mental management relatively highly affect goal setting as a subordinate concept of psychological skills. It is also found that personal relations and mental management are related to harmony of teams, and mental management affects mental state and willpower and training management affects anxiety control.

이단평행봉의 Ray 동작 시 숙련도에 따른 운동학적 차이

서연희(Yeon Hee Seo),이주형(Joo Hyung Lee),이석희(Suk Hee Lee) 한국여성체육학회 2011 한국여성체육학회지 Vol.25 No.3

The purpose of this study was to analyze kinematic factors of the Ray that is an uneven parallel bar skill in gymnastics. For this study, four national woman gymnasts participated in this experiment and they were divided into two groups (skilled and less skilled group) by means of performance scores. The two digital video cameras (Sony, PD-150) were used to record motions, and Kwon3D 3.1 was used to process data and analyze factors. The results were as follows: First, in down swing phase the width of swing for Skilled Group (SG) was greater than Less skilled group LG), that is, a vertical velocity in upward swing increased, and the Cog of SG was greater than LG at a release, that is, SG secured a maximum height at an air phase. Second, at an air phase and a landing, horizontal displacement of SG was less than LG, but vertical displacement of SG was greater than LG. This means that SG secured a maximum height with a body projecting, but LG landed at near a bar with body projecting ahead. Third, at release, SG represented a rapid vertical velocity and a large shoulder joint angle which was staple factors with a body rising upward made a body stable landing to compare with the LG, but LG did the opposite results. In conclusion, using rotation gained with a wide down swing at release, SG made extension of shoulder joint, a high vertical position and rapid velocity. Therefore increasing an air position made a good air rotation and stable landing.

중학교 체육교과 학습자의 내적동기와 수업몰입 및 수업만족감의 관계

서연희(YeonHee Seo) 한국스포츠교육학회 2016 한국스포츠교육학회지 Vol.23 No.2

이 연구의 목적은 중학교 체육교과 학습자들을 대상으로 내적동기와 수업몰입 및 체육수업 만족감의 관계를 규명하는 것이다. 연구대상은 J시 소재 남녀공학중학교에 재학하고 있는 중학생들이며 편의표본추출법(convenience sampling method)을 이용하여 287명의 자료를 수집했다. 수집된 자료를 바탕으로 기술통계, 탐색적 요인분석(EFA), 다변량분석(MANOVA), 상관분석, 단계적(step-wise) 회귀분석을 실시했다. 이에 대한 연구결과는 다음과 같다. 첫째, 내적동기의 하위요인인 재미 수준에 따라서 성과만족과 지도만족은 모두 유의한 차이가 나타났지만 긴장 수준에 따른 성과 및 지도만족의 유의한 차이는 없는 것으로 나타났다. 유능감 수준에 따라서 성과만족은 유의한 차이가 나타났지만 지도만족은 유의한 차이가 없는 것으로 나타났다. 둘째, 체육수업몰입의 하위요인인 일치 몰입의 수준에 따라서 성과만족과 지도만족은 모두 유의한 차이가 나타났다. 자기목적-통합, 몰두, 통제성 수업몰입의 수준에 따라서 성과만족은 유의한 차이가 나타났지만 지도만족은 유의한 차이가 나타나지 않았다. 셋째, 내적동기와 수업몰입은 수업만족도와 밀접한 상관관계를 형성하는 것으로 나타났으며 재미와 유능감, 수업몰입의 일치, 몰두감 자기목적적 통합 몰입은 성과만족에 영향을 미치는 것으로 나타났다. 또한, 수업몰입의 일치, 몰두감, 유능감은 지도만족에 영향을 미치는 것으로 나타났다. 본 연구를 토대로 후속 연구에서는 수업만족감 변수의 교사변수와 인지적 측면에서 다차원적으로 수업만족의 추가적인 규명이 필요하다는 결론을 얻었다. The purpose of this study was to investigate the relationship between intrinsic motivation PE class flow and PE class satisfaction among physical education curriclum learners. The subjects(N=287) were selected by convenience sampling method from middle schools in J All data were analyzed by descriptive statistics, MANOVA, correlation analysis and step-wise regression analysis. The results of this study were as follows: First, there were significant differences in performance satisfaction and coaching satisfaction according to the joy level, a subfactor of intrinsic motivation, but, not, according to the tension level. Second, there were significant differences in performance satisfaction and coaching satisfaction according to the agreement flow level, a subfactor of physical education flow. Also, the levels of self-purpose experience-integration commitment, challenge and control flow made significant differences in performance satisfaction, but, not in coaching satisfaction. Third, intrinsic motivation and class flow had a close correlation with class satisfaction and joy and competence of intrinsic motivation, agreement of class flow, the feeling of immersion, and self-purpose experience-integration commitment had effects on performance satisfaction. The agreement of class flow, the feeling of immersion and competence of intrinsic motivation had an influence on coaching satisfaction. Finally, We discussed the some findings based on these results, and this study suggested follow-up studies. Based on this study, we drew a conclusion that additional and multidimensional investigation on teacher variables of class satisfaction variables and PE class satisfaction from the cognitive aspects should be made in the following studies.

간호대학생의 인공지능에 대한 지식, 인식 및 수용태도가 인공지능 이용의도에 미치는 영향

서연희(Yon Hee Seo),조경아(Kyong Ah Cho) 한국간호연구학회 2022 한국간호연구학회지 Vol.6 No.3

Purpose : Artificial intelligence (AI) has potentials to significantly transform a role of nurses and revolutionise practices of healthcare systems. The aim of this study is to identify influences of AI Knowledge, Perception, and Acceptance Attitude on nursing students’ Intentions to use AI-based healthcare technologies. Methods : The participants included 241 nursing students in Gyeonggi-do and Jeollanam-do, with data collected from 30 May to 30 June 2022 using self-reported questionnaires. The data were analyzed using the SPSS/WIN 25.0 program, with independent t-tests, one-way ANOVA, Pearson’s correlations, and multiple linear regression. Results : The results revealed that AI Knowledge positively correlated with AI perception(r=.40, p<.001), and Acceptance Attitude toward AI(r=.17, p=.007). AI perception positively correlated with AI Acceptance Attitude(r=.53, p <.001), and Intentions to use AI(r=.46, p<.001). AI Acceptance Attitude also positively correlated with Intentions to use AI(r=.52, p<.001). The factors influencing Intentions to use AI-based healthcare technologies included AI Perception(β=.30) and Acceptance Attitude AI Acceptance Attitude(β=.38). The adjusted R2 was .318. Conclusions : It is necessary to develop systematic educational programs on AI technologies and form an organizational culture to improve nursing clinical competency and professionalism for nursing students in healthcare setting.

간호대학생의 정신건강정보이해능력과 인권감수성이 정신장애인에 대한 사회적 거리감에 미치는 영향

서연희(Seo Yonhee),조경아(Cho Kyongah) 학습자중심교과교육학회 2020 학습자중심교과교육연구 Vol.20 No.21

본 연구는 간호대학생의 정신건강정보이해능력과 인권감수성이 정신장애인에 대한 사회적 거리감에 미치는 영향을 확인하고자 시도되었다. 연구대상자는 서울특별시와 경기도 소재의 5개 대학에 재학중인 간호대학생 208명을 모집하였으며, 자료수집은 2019년 5월 28일부터 6월 11까지 자기기입식 설문지를 이용하여 수집하였다. 본 연구결과, 정신건강정보이해능력은 16점 만점에 11.50점, 인권감수성은 75점 만점에 55.03점, 사회적 거리감은 40점 만점에 23.85점이었으며, 사회적 거리감에 영향을 미치는 요인으로 인권감수성(β=.301, p<.01), 정신질환 교육경험(β=306, p<.01), 정신건강정보이해능력(β=125, p=.05)으로 확인되어 전체 설명력은 19.5%로 나타났다. 이에 정신장애인에 대한 사회적 거리감을 완화시키기 위해서는 간호대학생의 정신건강정보이해능력과 인권감수성을 향상시키고 체계적인 교육 전략과 교육 프로그램을 개발하여 적용할 것을 제언한다. This study was to investigate the effects of mental health literacy and human rights sensitivity of nursing students on social distance in people with mental disorders. The participants comprised 208 nursing students from 5 universities located in Seoul and Gyeonggi-do. Data were collected using self-report questionnaires from May to June 2019. The data were analyzed using descriptive statistics, Independent t-tests, Pearson s correlation coefficient, and stepwise multiple regression. As a result of this study, mental health literacy was 11.50 out of 16, human rights sensitivity was 55.03 out of 75, and social distancing was 23.85 out of 40. The factors affecting social distance were human rights sensitivity (β=.301, p<.01), mental illness education experience (β=306, p<.01), and mental health literacy (β=125, p=.05); the explanatory power of the model was 19.5%. Therefore, in order to alleviate social distance towards people with mental disorders, it is necessary to improve mental health literacy and human rights sensitivity of nursing students, and the systematic education strategy and education program should be developed and applied.

기계체조 선수의 성취목표성향 자기관리 및 수행전략의 구조관계 분석

서연희(Seo, Yeon-Hee) 한국체육과학회 2014 한국체육과학회지 Vol.23 No.5

This research drew a purpose through the Analysis of The Structure Relation Among Achievement Goal Orientation, Self-Regulation, and Performance Strategy in Gymnast. First, it was statistically significant influence of structural relationships achievement goal orientation and self-management of the gymnast. Second, there was a statistically significant influence of the structural relationship between self-management and gymnast performing strategy. Third, a statistically significant influence perform structural relationship between achievement goal orientation and strategy of the gymnast. It can be seen that the effect to improve the performances of the various strategies philosophical psychological aspects of the nature of the sport exercises to strengthen and maintain individual player in the light these findings. Based on this study, it is suggested that more specific achievement goal orientation, according to age and gender sub-factors between self-management and the need to perform strategic analysis to see events as players age am low in subsequent studies.

대학 학생선수의 운동스트레스, 자기관리 및 중도포기의 관계모형 검증

서연희(Seo, Yeon-Hee),정구인(Jung, Koo-In) 한국체육과학회 2015 한국체육과학회지 Vol.24 No.3

The purpose of this study is to verify a causal relation between exercise stress of university student athletes, their self-management and their giving-up in the middle. For data processing this study analyzed a total of 304 questionnaire copies with SPSS WIN 18.0 by conducting confirmatory factor analysis, reliability analysis, frequency analysis, descriptive statistics, and correlation analysis. To examine the structural relation of the study model, this study employed LISREL 8.80 to conduct confirmatory factor analysis and structural equation modeling (SEM). The analysis results are presented as follows: First, exercise stress of university student athletes significantly influenced their giving-up in the middle (excessive training, anxiety over the future, economic burden, loss of self-esteem, negative friendship, loss of interest, and unfair hierarchical structure). Secondly, self-management of university student athletes (body management, interpersonal relationship management, training management, and mentality management) significantly influenced their giving-up in the middle (excessive training, anxiety over the future, economic burden, loss of self-esteem, negative friendship, loss of interest, and unfair hierarchical structure).

운동선수의 은퇴기대, 심리적 위기감, 은퇴준비수준 및 재사회화 간의 구조관계

서연희(Seo, Yeon-Hee) 한국체육과학회 2012 한국체육과학회지 Vol.21 No.4

The purpose of this research was to examine the structural relationship among athletes’ retirement expectation, psychological sense of crisis, level of retirement preparation and re-socialization, and to provide them with a practical method for their organized preparation for retirement and the efficient adaptation of themselves to a new environment after retirement. For the research, the survey targeted the elite athletes in the Chonbuk province. For the account of research target’s characteristics and questionnaires, frequency analysis, reliability analysis and correlation analysis were used with SPSS WIN 18.0. In order to verify the structural relationship of the study modeling, confirmative factor analysis and structural equation modeling were operated with LISREL 8.80. The result of this study was as follows: First, athlete’s retirement expectation was statistically significant to a psychological sense of crisis. Second, the retirement expectation made a statistically meaningful influence on the level of retirement preparation. Third, the retirement expectation was influential to re-socialization with statistical significance. Fourth, his/her psychological sense of crisis was not statistically meaningful to re-socialization. Last, his/her level of retirement preparation statistically had a substantial impact on re-socialization.