염화칼슘 주사량이 품종별 암사슴의 뿔 발생 유도에 미치는 영향

김상우 ( Sang Woo Kim ),이승수 ( Seung Soo Lee ),최순호 ( Sun Ho Choi ),상병돈 ( Byung Don Sang ),김영근 ( Young Geun Kim ),상병찬 ( Byong Chan Sang ),서길웅 ( Kil Woong Seo ),문상호 ( Sang Ho Moon ) 한국동물자원과학회 2008 한국축산학회지 Vol.50 No.3

맥주박 첨가 섬유질 배합사료가 거세흑염소의 생산성 및 영양소 이용율에 미치는 영향

최순호(Sun Ho Choi),황보순(Soon Hwangbo),김상우(Sang Woo Kim),상병돈(Byung Don Sang),김영근(Young Keun Kim),조익환(Ik Hwan Jo) 한국초지조사료학회 2006 한국초지조사료학회지 Vol.26 No.4

본 연구는 맥주박을 첨가한 섬유질 배합사료가 육성기 거세흑염소의 생산성과 영양소 이용율에 미치는 영향을 조사하여 맥주박의 사료가치를 평가하고자 실시하였다. 공시축은 사양시험은 거세흑염소 40두를 4처리구(관행사료구, 맥주박 20, 30 및 40% 첨가구)로 나누어 처리구당 10두씩, 소화율 시험에서는 처리구당 3두씩 완전임의 배치하여 시험을 실시하였다. 그 결과는 다음과 같다. 맥주박 첨가 수준별 섬유질 배합사료의 일반 조성분은 건물 함량이 62.35~66.02%, 조단백질 함량은 14.49~15.36%의 범위로 나타났으며, ADF와 NDF 함량은 28.24~29.08 및 53.27~54.85%로 나타나 맥주박 첨가 비율에 따른 섬유소 함량의 차이는 없었으나, NFC 함량은 맥주박 첨가 비율이 높을수록 NFC 함량이 낮아졌다. 일당증체량은 대조구가 맥주박 첨가구보다 유의하게 높았다(p<0.05). 건물섭취량, 유기물섭취량, NFC 섭취량은 맥주박 20% 첨가구가 가장 높았고(p<0.05), 조단백질과 가소화 조단백질 섭취량은 맥주박 첨가구가 대조구 보다 높은 경향이었다. 건물, 유기물, NDF 소화율은 대조구가 맥주박 첨가구 보다 유의하게 높았으며(p<0.05), 조단백질 소화율은 맥주박 첨가구가 대조구 보다 높은 경향이었고, 질소 축적율은 대조구 보다 맥주박 첨가구가 높은 경향이었다. This study was conducted to investigate the effects of Total Mixed Ration (TMR) with wet brewer’s grain on feed intake, nutrient digestibility and nitrogen retention of castrated Korean black goat. For feeding trial, forty castrated Korean black goats were divided into four treatment groups, which were fed TMR containing 20, 30, 40% of wet brewer’s grain. Fo r digestibility trial, twelve castrated Korean black goats were allotted to treatments in four groups of three goats. Results are summarized as follows. Dry matter (DM) contents of TMR with wet brewer’s grain was 63.35~66.02%, that of crude protein was 14.49~15.36%, Acid detergent fiber (ADF) and Neutral detergent fiber (NDF) were 28.24~29.08, 53.27~54.85%, each. These were not different by supplemental level of brewer’s grain in TMR. However, Non-fibrous carbohydrate (NFC) contents were lower according to increase wet brewer’s grain in TMR. Average daily gain of control group were higher than those from TMR with wet brewer’s grain (p<0.05). Intake of DM, Organic matter (OM) and NFC of TMR supplemented with 20% of brewer’s grain were higher than those of other treatments (p<0.05). Crude protein (CP) and digestible CP intake of TMR diets supplemented wet brewer’s grain was intended to highe r than those of a control group. Digestibilities of DM, OM and NDF in control group were higher than those from TMR with wet brewer’s grain (p<0.05). Digestibilities of CP of TMR with supplemented wet brewer’s grain was tend to be higher than those of a control group. Nitrogen retention of TMR supplemented wet brewer’s grain was tend to be somewhat higher compared to the control group.

맥주박을 첨가한 섬유질 배합사료가 번식흑염소의 영양소 이용율에 미치는 영향

최순호(Sun Ho Choi),황보순(Soon Hwangbo),김상우(Sang Woo Kim),상병돈(Byung Don Sang),김영근(Young Keun Kim),조익환(Ik Hwan Jo) 한국초지조사료학회 2006 한국초지조사료학회지 Vol.26 No.3

This study was conducted to investigate the effects of Total Mixed Ration(TMR) with wet brewer’s grain on feed intake, nutrient digestibility and nitrogen retention of breeding Korean native goat. Twelve breeding Korean native goats were divided into four treatment groups, which were fed TMR containing 20, 30, 40% of wet brewer’s grain and a control group fed concentrate feed and hay, respectively. Results are summarized as follows. Dry matter contents of TMR with wet brewer’s grain was 61.46-65.81%, that of crude protein was 14.42-15.59%, ADF and NDF were 28.32-28.52, 53.46-54.16%, each. These were not different by supplemental level of brewer’s grain in TMR. However, NFC contents were lower according to increase wet brewer’s grain in TMR. Dry matter intake of TMR supplemented with 20% of brewer’s grain was tend to be higher than those of control group. CP and digestible CP intake of TMR diets supplemented wet brewer’s grain was intended to high er than those of a control group. NFC and digestible NFC intake were significantly higher in control group than in WBG40 treatment(p〈0.05). Digestibilities of DM, ADF, NDF and NFC in control group were higher than those from TMR with wet brewer’s grain(p〈0.05). Digestibilities of CP of TMR with supplemented wet brewer’s grain was tend to be higher than those of a control group. Nitrogen retention of TMR supplemented wet brewer’s grain was tend to be somewhat higher compared to the control group.

섬유질배합사료 내 조단백질 수준이 임신초기 흑염소의 건물섭취량, 소화율 및 질소출납에 미치는 영향

황보순(Soon Hwangbo),최순호(Sun Ho Choi),이성훈(Sung Hoon Lee),김상우(Sang Woo Kim),김영근(Young Keun Kim),상병돈(Byung Don Sang),조익환(Ik Hwan Jo) 韓國草地學會 2007 한국초지조사료학회지 Vol.27 No.2

본 연구는 섬유질배합사료의 조단백질 수준이 임신초기 흑염소의 사료섭취량, 영양소 소화율 및 질소 축적율에 미치는 영향을 조사하여 임신흑염소의 적정 조단백질 수준을 구명하기 위하여 실시하였다. 공시축은 임신초기흑염소 12두를 4처리구(관행사료구, 조단백질 10, 12 및 15%)로 나누어 처리구당 3두씩 대사케이지에 완전임의 배치법으로 배치하여 시험을 실시하였으며 그 결과는 다음과 같다. 섬유질배합사료의 일반 조성분은 건물 함량이 89.0~91.0%를 나타내었고, ADF와 NDF 함량은 18.87~19.85 및 53.41~54.80%로 나타나 조단백질 수준에 따른 섬유소 함량의 차이는 없었다. 조단백질 함량은 TMR10, TMR12 및 TMR14구가 각각 10.61, 12.15 및 14.97% 이었다. 하지만, NFC 함량은 섬유질배합사료내 단백질수준이 증가함에 따라 감소하였다. 건물섭취량, 영양소 섭취량 및 가소화 영양소 섭취량은 TMR15와 대조구가 TMR10과 TMR12구 보다 유의하게 높았으며(p<0.05), 대사체중당 건물섭취량과 체중에 대한 건물섭취비율에서도 대조구와 TMR15구가 유의하게 높았다(p<0.05). 건물소화율은 69.01~70.33% 범위로 나타났다. 에테르 추출물 소화율은 세 수준의 단백질을 함유하는 섬유질배합사료군이 대조군 보다 유의하게 높았으나(p<0.05), 섬유질배합사료군간에는 유의한 차이가 나타나지 않았다. 질소축적량 및 축적율에서는 TMR15구와 대조구가 다른 처리구 보다 유의하게 높게 나타났다(p<0.05). 이상의 결과에서 임신초기 흑염소사료 내 증가하는 수준의 조단백질 함량은 건물섭취량 및 질소 축적을 증가시켰고, 특히 TMR 사양에서 임신초기 흑염소의 조단백질수준은 적어도 15% 이상은 되어야 할 것으로 추정된다. This study was conducted to determine the effects of different levels (10, 12 and 15%) of crude protein (CP) in total mixed ration (TMR) on dry matter intake, digestibility and nitrogen balance of Korean black goats in the stage of early pregnancy and to obtain information on their optimal dietary levels of CP. In the present study, 12 Does of Korean black goats in the early pregnancy were allotted to four unreplicated groups by dietary level of CP and then they were housed in individual metabolism cages with completely randomized design throughout 30 days with 20 days adaptation and 10 days collection periods. Does in Control were fed a conventional diet and does in TMR10, TMR12 and TMR15 were fed a diet adjusted to about 10, 12 and 15% CP, respectively. Dry matter(DM) contents ranged from 89 to 91% in treatments. There were no differences for fiber contents among three CP levels of TMR, showing that ADF and NDF had 18.87 to 19.85, and 53.41 to 54.80, respectively. Crude protein contents for three TMR treatments had 10.61, 12.15 and 14.97%, respectively. However, non-fibrous carbohydrate (NFC) contents decreased with increasing CP levels in treatments. Meanwhile, Intakes of DM, nutrients and digestible nutrients were significantly (p<0.05) higher in TMR15 and control than in TMR10 and TMR12. Moreover, DM intake per metabolic body weight and their ratio per body weight was significantly (p<0.05) higher for control and TMR15 than other treatments. DM digestibility was not significantly different among treatments, but ether extract digestibility of treatments was significantly (p<0.05) higher than that of control, but there was no significant difference among treatments. Nitrogen retention significantly (p<0.05) increased with increasing CP levels in TMR, and TMR15 was highest among treatments. Our results showed that the increasing CP levels in TMR increased DM intake and nitrogen retention and suggested that the optimal dietary CP levels under TMR feeding system in early pregnant Korean black goats could be estimated for at least 15%.

산지 초지 유형이 번식 흑염소의 생산성 및 영양소 이용율에 미치는 영향

황보순(Soon Hwang bo),최순호(Sun Ho Choi),김상우(Sang Woo Kim),김영근(Young Keun Kim),상병돈(Byung Don Sang),권두중(Doo Jung Kwon),조익환(Ik Hwan Jo),최재국(Jae Gook Choi) 韓國草地學會 2007 한국초지조사료학회지 Vol.27 No.1

본 연구는 산림부산물과 국내 유기농부산물의 사료가치와 유기흑염소 생산에 기초적인 자료를 얻고자 산지 초지유형에 따른 흑염소의 사료 섭취량, 소화율 및 질소 축적율에 미치는 영향을 조사하였다. 공시축은 한국재래흑염소이었으며, 사양 시험에서는 흑염소 40두를 4처리구(개량목초구, 유기목초구, 산야초구, 수엽류구)로 나누어 처리구당 10두씩, 소화율 시험에서는 처리구당 3두씩 완전임의 배치하여 시험을 실시하였다. 그 결과는 다음과 같다. 사양시험기간 동안의 총증체량과 일당증체량은 개량목초구, 유기목초구, 산야초구, 수엽류구 순으로 낮아졌다(p<0.05). 건물섭취량, 가소화 건물섭취량, 건물소화율 및 질소축적율은 개량목초구가 가장 높았다(p<0.05). 이상의 결과를 종합해 보면, 산림 부산물을 조사료원으로 이용하고 국내 유기농부산물을 보충사료로 급여하여 방목사육된 유기흑염소는 다소 생산성이 낮으나 안정성이 높기 때문에 육용보다는 약용형태의 소비가 바람직하다고 사료되며, 낮은 생산성을 높이기 위해선 보충사료에 추가적인 영양소 공급이 반드시 필요하다고 판단된다. This study was conducted not only to evaluate the feed value for forests and organically produced-agricultural byproducts, but also to determine effects of different hilly pasture types on feed intake, nutrient digestibility and nitrogen retention in Korean black goats. Forty black goats were employed in the feeding trial and they were separated into four treatments (forages grown in chemical fertilizer, organic forages, native plants and browses) with 10 goats for each treatment. Furthermore, 12 goats, for nutrient digestibility trial, were allotted to four treatments of three goats per treatment with a randomized complete block design. Total body weight gain and average daily gain were significantly (p<0.05) decreased in the order of forages grown in chemical fertilizer, organic forages, native plants and browses throughout the trial. Dry matter intake (DM), digestible DMI, DM digestibility and nitrogen retention were highest in forages treatment grown in chemical fertilizer (p<0.05). From the above results, Organic Korean black goats fed forest byproducts as forage source and domestic organically produced-agricultural byproducts as supplemental diets were somewhat lowered in their performance. However, it showed high stability in the sustainable farming of organic Korean black goats. As the result, the consumption of organic Korean black goats might be more desirable for medicinal use rather than for meat as a table food. The way to increase the low performance of organic Korean black goats for supplemental diets might be the additional supply of lacking nutrients for supplemental diets.

육우의 혈액단백질 및 효소의 유전적 변이체

상병찬,류승희,상병돈 충남대학교 농업과학연구소 1995 농업과학연구 Vol.22 No.1

This study was conducted to examine the genetic variants of the blood proteins and enzymes in beef cattle breeds, Hereford, Angus and Sharolais reared at the Daekwanryuong Branch of the National Livestock Research Institute. Genetic polymorphisms of transferrin(Tf), post-transferrin2(pTf-2), albumin(Alb), post-albumin (pAlb), ceruloplasmin(Cp), amylase-I(Am-I) and hemoglobin(Hb) in blood were analyzed by the methods of PAGE(polyacrylamide gel electrophoresis) and STAGE(starch gel electrophoresis). The results obtained from this study were summarized as follows: 1. Tf and pTf-2 locus assumed to be controlled by codominant alleles, A. D_1, D_2 and E allele for Tf, F and S allele for pTf-2. In genotype frequencies, 25% and 90% for Tf D_1D_2 and pTf-2 SS in Hereford, 25% and 100% for Tf AD_1 and pTf-2 FF in Angus, 50% for Tf D_1D_1 and pTf-2 FS in Sharolais were found to have the highest frequency, respectively. In gene frequencies, 0.400 and 0.900 for Tf E and pTf-2 S allele in Hereford, 0.678 and 0.607 for Tf D_1 and pTf-2S in Sharolais were appeared to have the highest frequency. 2. Alb and pAlb locus assumed to be controlled by codominant alleles, only A allele for Alb, F and S allele for pAlb. In genotype frequencies, 70% for pAlb SS in Hereford, 90% for pAlb FF in Angus and 57.15% for pAlb SS in Sharolais were found to have the highest frequency. In gene frequencies, 0.825 and 0.750 for pAlb S in Hereford and Charolais, 0.900 for pAlb F in Angus were found to have the highest frequency. 3. Cp and Am-I locus appeared to be controlled by two alleles, F and S allele for Cp, B and C allele for Am-I. In genotype frequencies, 100% and 65% for Cp FF and Am-I BB in Hereford, 45% and 85% for Cp FF, and Am-I CC in Angus, 50% and 64.29% for Cp FF and Am-I BC in Sharolais were found to have the highest frequency. Gene frequencies were 1,000, 0.600 and 0.750 for Cp F in Herehord, Angus and Sharolais, 0.800, 0.875 and 0.680 for Am-I B, C and C allele in Hereford, Angus and sharolais, respectively. 4. Hb locus assumed to be controlled by codominant alleles, only A allele in Hereford and Angus, A and B allele in Sharolais. Genotype frequencies were 57.14% and 42.86% for Hb AA and AB in Sharolais, and gene frequencies were 0.785 and 0.215 for Hb A and B in Sharolais.

Holstein 종 유우의 우유단백질의 유전적 다형과 생산형질간의 연관성에 관한 연구

안병석,상병찬,상병돈,조윤연 한국낙농학회 1993 韓國酪農學會誌 Vol.15 No.4

本 硏究는 乳蛋白質의 遺傳的 標識(genetic marker)를 乳牛의 遺傳的 改良을 위한 유우의 登錄 및 選拔 補助資料로 활용하고자 1992년 國立種畜院에서 사육중인 Holstein종 159두에서 각각 유즙 시료를 채취하여 Polyacrylamide gel(PAGE)에 의한 전기영동(electrophoresis) 방법에 의하여 αs₁-casein(αsl-CN), β-casein(β-CN), κ-casein(κ-CN) 및 β-lactoglobulin(β-LG)의 遺傳子型을 조사하고, 이들 蛋白質의 遺傳的 多型(genetic polymorphisms)과 泌乳形質 및 乳組成分含量과의 연관성을 分析하여 얻어진 결과는 다음과 같다. 蛋白質座位의 遺傳子型을 분석한 결과 αs₁-CN BB遺傳子型, β-CN AA遺傳子型, κ-CN A 遺傳子型 및 β-LG AB遺傳子型의 出現頻度는 각각 77.87%, 84.28%, 71.70% 및 49.10%로 同一蛋白質內의 다른 遺傳子型 보다 높게 나타났다. 분산 분석결과 乳蛋白質의 遺傳子型들은 泌乳形質인 乳量, 乳脂量 및 蛋白質量과 乳組成分인 乳脂率, 蛋白質含量 및 총고형분 함량에서 유의적인 연관성이 인정되었다. 泌乳形質에 있어서는 αs₁-CN 座位의 BB遺傳子型이 유량, 乳脂量 및 蛋白質量에서 BC遺傳子型 보다 유의적으로 높았고, κ-CN座位의 AA형은 AB 및 BB遺傳子型보다 유량에서 유의적인 연관성이 인정되었으며, β-LG座位의 AA 및 AB형은 유량에서 BB형 보다 높게 나타났다. 乳組成分含量에 있어서는 κ-CN座位의 BB遺傳子型은 乳脂率 및 乳蛋白質含量에서 κ-CN座位의 AA 및 AB遺傳子型보다 유의적으로 높게 나타났으며, β-LG座位의 AA遺傳子型은 乳脂率에서 β-LG座位의 AB 및 BB遺傳子型보다 유의적으로 높게 추정되었다. 이상의 연구 결과를 종합하여 볼 때 泌乳形質인 乳量, 乳脂量 및 蛋白質量을 증대시키기 위하여서는 αs1-CN座位의 BB遺傳子型, κ-CN 및 β-LG座位의 AA傳子型을 선발하는 것이 보다 바람직할 것으로 사료되었으며, 乳組成分인 乳脂率 및 蛋白質含量을 높히기 위하여서는 κ-CN座位의 BB遺傳子型과 β-LG座位의 AA遺傳子型을 選拔하는 것이 보다 유리할 것으로 생각되었다. To apply genetic markers of milk proteins to dairy cow registration and selection aids for genetic improvement, genotypes controlling the four type of milk protein loci of αs1-casein(αs₁-CN), β-casein(β-CN), κ-casein(κ-CN), and β-lactoglobulin(β-LG) in milk from a total of 159 Holstein lactating cows reared at Korean National Animal Breeding Station in 1992 were detected by polyacrylamide gel(PAGE) electrophoresis, and the genetic structure of the population was analyzed using genotypic frequencies of milk protein type. The effects of genetic polymorphisms of milk proteins on lactation traits and milk compositions were analyzed statistically by least square analysis. The results obtained in this experiment was as follows ; The observed frequencies of the αs₁-CN BB, β-CN AA, κ-CN AA and β-LG AB genotypes were founded to be very higher than that of other genotypes in identified proteins as 79.87%, 84.28%, 71.70% and 49.10%, respectively. According to the results of analysis of variance, the genotypes of the αs₁-CN, β-CN, κ-CN and β-LG were significantly different for milk, fat and protein yield in lactation traits, and for fat, protein and total solid percentage in milk. On lactation yields, the αs_1-CN BB genotype was higher in milk, fat and protein yield more than αs₁-CN BC genotype, and the κ-CN AA genotype was higher in milk and protein yield more than κ-CN BB and AB genotypes, and the β-LG AA and AB genotypes were higher in milk yield more than β-LG BB genotype at 5% level of significant difference, respectively. On milk compositions, the κ-CN BB genotype was higher in fat and protein percentage more than κ-CN AA and AB genotypes, and β-LG AA genotype was higher in fat percentage more than β-LG AB and BB genotype at 5% level of significant difference, respectively. As a consequence, the milk, fat and protein yield could be improved by the selection of cows which express the αs₁-CN BB, κ-CN AA and β-LG AA genotypes, and the fat and protein percentage could be improved by the selection of cows which express the κ-CN BB and β-LG AA genotypes.

Holstein 종 젖소의 혈청단백질 및 효소의 유전적 다형현상

이창수,상병찬,류승희,상병돈 한국낙농학회 1996 韓國酪農學會誌 Vol.18 No.2