누드 마우스에서 Encephalomycocarditis D 변이종 바이러스의 인슈린 의존성 당뇨병의 유발

백상기,윤지원,신승일 ( Sang Gi Paik,Ji Won Yoon,Seung Il Shin ) 생화학분자생물학회 1986 BMB Reports Vol.19 No.4

The possibility of the host thymus-dependent functions in the development of insulin-dependent diabetes mellitus was investigated in athymic nude (nu/nu) mice and euthymic heterozygous (+/nu) littermates of CD-1 origin, injecting either the multiple subdiabetogenic doses of streptozotocin (STZ) or the D-variant of encephalomyocarditis (EMC-D) virus. A single high dose of STZ caused a rapid hyperglycemia in both +/nu and nu/nu male mice. However, multiple low doses of STZ (30 or 35 ㎎/㎏ b.w./d, for 5 days) induced delayed hyperglycemia only in +/nu male mice ($gt;85%), and not in nu/nu male mice ($lt;17%). It clearly shows inhibitory effect of the nude gene in induction of diabetes in CD-1 mice by multiple low doses of STZ. The EMC-D virus produced hyperglycemia and insulitis in both +/nu and nu/nu male mice (76% diabetic and 73% diabetic, respectively). This shows that the immunological competence of the host may have no effect on diabetogenesis by EMC-D virus. Independent of their thymic function, female mice were quite resistant to the diabetogenic effect of the EMC-D virus. In EMC-D virus infected diabetic mice, the gross necrosis and insulitis were correlated only with the degree of hyperglycemia, regardless of the presence or absence of thymus. These results indicate that diabetes induction by EMC-D virus is not mediated by thymus (T-cell)-dependent processes.

Induction of Insulin-Dependent Diabetes Mellitus in Athymic Nude Mice by a Diabetogenic Strain (D-Variant) of Encephalomyocarditis Virus

백상기,윤지원,신승일,Paik, Sang-Gi,Yoon, Ji-Won,Shin, Seung-Il 생화학분자생물학회 1986 한국생화학회지 Vol.19 No.4

면역 기구인 흉선이 없는 유전적 면역 결핍인 누드 마우스(nu/nu)와 흉선을 정상으로 갖고 있은 +/nu CD-l 계통 마우스에 당뇨병 유발원으로 streptozotocin (STZ)가 encephalomyocarditis D 변이종 바이러스 (EMC-D)를 이용하여 인슈린 의존성 당뇨병 발명에 흉선(T임파구)이 어떤 영향을 미치는가를 조사했다. STZ를 고농도로 일회 주사했을 경우 +/nu와 nu/nu 웅성 CD-1 마우스에서 모두 혈중 포도당 농도가 높아지고 인슈린량이 결핍되는 당뇨병이 발병 하였으나 이 농도를 5회에 나누어 5일 동안 주사하는 저농도 투여법을 사용했을 때 +/nu CD-1 마우스에서는 당뇨병이 생기나, nu/nu CD-1 마우스에서는 당뇨병의 발병이 억제됨을 보았다. 이 결과들로 STZ의 저농도 투여법에 의한 당뇨병 유발은 면역 기그가 작용하여 이루어진다는 가설을 확인할 수 있었다. 한편 EMC-D 바이러스는 +/nu와 nu/nu CD-1 마우스 모두에서 당뇨병을 발병시켰고 이로서 nu/nu CD-1 마우스의 면역 결핍이 EMC-D 바이러스에 의한 당뇨병 생성에 하등의 영향을 미치지 않는다는 것을 알았다. 인슈린을 생성하는 취장의 변화에 대한 조사 결과 흉선 유무에 관계없이 취장내 랑겔한스섬의 세포들이 심한 Necrosis와 insulitis를 보여주었고 이는 당뇨병의 정도와 일치하였다. 이 결과들은 EMC-D 바이러스에 의한 당뇨병 발병은 T임파성 면역 기구가 작용하지 않는다는 것을 보여준다. The possibility of the host thymus-dependent functions in the development of insulin-dependent diabetes mellitus was investigated in athymic nude (nu/nu) mice and euthymic heterozygous (+/nu) littermates of CD-1 origin, injecting either the multiple subdiabetogenic doses of streptozotocin (STZ) or the D-variant of encephalomyocarditis (EMC-D) virus. A single high dose of STZ caused a rapid hyperglycemia in both +/nu and nu/nu male mice. However, multiple low doses of STZ (30 or 35 mg/kg b.w./d, for 5 days) induced delayed hyperglycemia only in +/nu male mice (>85%), and not in nu/nu male mice (<17%). It clearly shows inhibitory effect of the nude gene in induction of diabetes in CD-l mice by multiple low doses of STZ. The EMC-D virus produced hyperglycemia and insulitis in both +/nu and nu/nu male mice (76% diabetic and 73% diabetic, respectively). This shows that the immunological competence of the host may have no effect on diabetogenesis by EMC-D virus. Independent of their thymic function, female mice were quite resistant to the diabetogenic effect of the EMC-D virus. In EMC-D virus infected diabetic mice, the gross necrosis and insulitis were correlated only with the degree of hyperglycemia, regardless of the presence or absence of thymus. These results indicate that diabetes induction by EMC-D virus is not mediated by thymus (T-cell)-dependent processes.

Molecular Genetic Studies of Korean Population - 4. Genetic Structure of Adenylate Kinase ( AK ) -

김영진,백상기,이정주,김종순,안광숙 ( Yung Jin Kim,Sang Gi Paik,Chung Choo Lee,Jong Soon Kim,Gwang Sook Ahn ) 한국유전학회 1987 Genes & Genomics Vol.9 No.3

Red cell adenylate kinase(AK) phenotype and gene frequency was determined by horizontal starch gel electrophoresis for 509 unrelated adult individuals living in the 6 provinces of Korea> Only one type of AK 1 phenotype was found for all individuals surveyed, thus the genes for AK systems were fixed for single allele, AK1. The frequencies of AK1 and AK2 were 1.000 and 0.000, respectively in Korea. In the literature data on the phenotypes and gene frequencies of the AK are widely scattered have been complied, and here certain trends in the AK2 gene distribution were considered.

갈겨니(Zacco temminki)의 진화에 관한 연구 VII.갈겨니 2 Type의 Mitochondrial DNA변이

이혜영,양서영,백상기,박창신,유성림,이성근,Lee, Hei-Yung,Yang, Suh-Yung,Paik, Sang-Gi,Park, Chang-Shin,Yu, Sung-Lim,Lee, Sung-Keun 한국통합생물학회 1988 동물학회지 Vol.31 No.3

한국산 담수 어류인 갈겨니(Zacco temminki)는 형태적으로 동일하거나 전기영동법에 의한 s-Mdh에 2type(MM과 MS type)이 있음을 (1987)이 주장하였으며 2type간 유전적 차이 정도를 분석한 결과 자매종으로 밝힌바 있다. 본 연구에서는 상기 결과를 토대로 2type의 mtDNA를 4개 집단에서 추출하여 11가지 제한 요소로 처리한 다음 fragment양상을 비교 분석하였다. 갈겨니 mtDNA의 전 genome크기는 약 16.7Kb였으며 frabment homolgy(F)에서 MS type 간 F값은 0.464,MM type간 F값은 0.762로 높은 값을 나타냈다. 또한 MM과 MS 이형간 평균 F값은 0.312(0.258-0.345범위)로 동형간보다 낮은 유사성을 나타내고 있다. 집단 또는 type간 nucleotide sequence divergence(p)를 산출한 결과 MS type 간의 P=0.128에 비해 동형간의 P값은 0.045로 매우 낮은 mtDNA sequece 변이를 나타내었다. 그러나 이형간인 2type평균 P값은 0.195(0.177-0.226범위)로 차이를 나타냈다. 이와 같은 2type간의 차이는 isozyme연구 분석 결과와 일치하고 있어 갈겨니 2type간의 종분화를 확신시키는 새로운 자료가 되었다. Mitochondrial DNAs of two Mdh allelotypes of the dark chub, Z. temmincki inhabiting in Korean fresh water, were analysed. Samples of each type were collected from four populations, and the fragment patterns for mtdNA of each type were explained from 7 of the eleven restriction enzymes with hexanucleotide recognition site. Genome size was approximately 16.7 kilobases. The highly typical mtdNA fragments of each type were discovered in digestion profiles produced by Eco RI and Pst I enzyrnes. The comparisons of restriction fragment patterns and relative digestion maps permitted the estimation of fragment homology (F) and nucleotide sequence divergence(p). Between the two identical types, sequence divergence(p) was 0.128(MS), and 0.045(MM), ; between the two different types, 0.195 (range 0.177-0.226). These result may provide a distinct difference more than the value derived from allozyrne analysis, and a powerful new molecular approach for assessing genetic-evolutionary relationship among fishes.

백작약 조다당분획에 의한 B 세포 증식의 특성

박혜란,함연호,이성태,백상기,조성기,Park, Hae-Ran,Ham, Yeon-Ho,Yee, Sung-Tae,Paik, Sang-Gi,Jo, Sung-Kee 대한면역학회 2001 Immune Network Vol.1 No.2

Background : Paeonia japonica Miyabe is a medicinal plant which has been widely used as a component of blood-building decoctions (Chinese medicinal concept : Bu-Xie). The immunopharmacological characteristics of the extract of Paeonia japonica (PJ) were investigated. Methods : The effects of fractions of PJ extract on lymphocyte proliferation were measured by $H^3$-thymidine incorporation assay. The proliferated lymphocyte subsets were analyzed in flow cytometry. The subset cell populations of spleen cells were separated by magnetic cell separation system, and their proliferation by the extract were investigated. The effect of the extract on antibody production was determined in mice challenged with sheep red blood cells (SRBC) using hemolytic plaque forming cell assay. Results : Spleen cells were proliferated by water extract of PJ. Polysaccharide fraction (PJ-P) of the extract was most active in the proliferation. It was found in flow cytometry that the lymphocyte subset proliferated by PJ-P was B cell population. Among the separated subset cell populations, T cell-depleted cell population and macrophage-depleted cell population were most proliferated by PJ-P. However, positively selected populations of B cells and T cells were not proliferated by PJ-P. These results indicate that B cell proliferation by PJ-P may require the assistance of macrophages or T cells. These results suggest that firstly PJ-P may stimulate macrophages or T cells, and then B cells are activated. The number of antibody-secreting cells was increased by administration of PJ-P in mice immunized with SRBC as a T-dependent antigen. Conclusion : These results suggest that macrophages and accessory cells are directly activated by PJ-P and then helper T cells and B cells are indirectly activated. As the results, immune responses might be coordinately improved. In conclusion, PJ-P, a polysaccharide of P. japonica, may be a characteristic immunostimulator, which is analogous to polysaccharides such as lentinan, PSK and ginsan.

한국인 집단에서 미토콘드리아 DNA Control Region의 염기서열 변이분석

유정현(Jeong-Heon Yoon),백상기(Sang-Gi Paik),김영상(Young-Sang Kim) 한국법과학회 2000 한국법과학회 학술대회 Vol.2000 No.1

D7S820 STR locus의 다형성

조남수(Nam Soo Cho),황정호(Jung-Ho Hwang),이영애(Young-Ae Lee),백상기(Sang-Gi Paik),한면수(Myun Soo Han) 한국법과학회 2005 한국법과학회 학술대회 Vol.2005 No.1