온도감응중합체 및 트립신 처리에 의한 중간엽줄기세포의 계대 배양 비교

배소현 ( So Hyun Bae,),박채운 ( Chae Woon Park ),손혜경 ( Hye Kyung Son ),전춘주 ( Choon Ju Jeon ),김호언 ( Ho Eon Kim ) 한국조직공학·재생의학회 2007 조직공학과 재생의학 Vol.4 No.3

Mesenchymal stem cells (MSCs) possess extraordinary proliferation and broad differentiation potential, drawing much attention from the fields of regenerative medicine and tissue engineering. These stem cell potentials, however, are known to steadily decrease during ex vivo expansion. Upon serial passage in vitro, MSCs exhibit significant aging-related transformation, the progression of which is presumably accelerated by the routine use of trypsin. In this study, we have investigated whether a new passage method with using RepCellTM, a temperatureresponsive polymer (TRP)-coated culture dish, is superior, in terms of proliferative potential, to the traditional trypsin-mediated method. The results showed that the cells passaged five times by trypsin treatment assumed much older cell morphology and grew more slowly than those passaged with RepCellTM, suggesting that trypsin might exert deleterious effects on proliferative potential of MSCs. In addition, DNA microarray-based differential transcriptional profiling revealed a notable, but not significant, difference in gene expression between these two cell populations, indicating a slow deposition of trypsin-mediated molecular changes. In conclusion, TRP-mediated passage would be a preferred method for ex vivo expansion of MSCs without risking damage to the stem cell properties.

초진시 사시각에 따른 굴절조절내사시의 임상양상

배소현(So Hyun Bae),최동규(Dong Gyu Choi) 대한검안학회 2008 Annals of optometry and contact lens Vol.7 No.1

목적: 굴절조절내사시의 초진 시 사시각에 따른 임상양상에 대해 알아보고자 하였다. 대상과 방법: 1년 이상 경과관찰한 굴절조절내사시 환자 52명을 대상으로 초진 시 사시각에 따라 발병연령, 안경착용 시작 연령, 굴절이상, 부등시, 약시 동반 및 굴절이상의 변화를 분석하였다. 결과: 초진 시 사시각은 원거리 평균 25.42 PD (10∼60 PD), 근거리 26.23 PD (10∼60 PD)이었고 원거리 사시각이 20 PD 미만 13명(25%), 20 PD 이상 40 PD 미만 30명(57.7%), 40 PD 이상 9명(17.3%)이었다. 발병연령은 평균 30.26개월(출생 직후∼7.5세)이었다. 발병연령은 사시각이 20 PD 미만일 때 평균 48.00개월, 20 PD 이상 40 PD 미만이 27.45개월, 40 PD 이상이 15.89개월로 사시각이 클수록 어린 나이에 발병하였다. 연평균 0.47 D의 원시가 감소하였으며 사시각에 따른 유의한 연간 원시 감소량의 차이는 없었다. 결론: 굴절조절내사시에서 사시각이 클수록 어린 나이에 발병하는 임상양상을 보여 내사시의 진단 및 치료 시 유의해야 하겠다. Purpose: To evaluate clinical features in patients with refractive accommodative esotropia according to the initial angle of esodeviation Methods: A total of 52 patients enrolled in retrospective study of refractive accommodative esotropia had been followed for at least 1 year. Several clinical characteristics were evaluated including age at onset, age when spectacles prescribed, cycloplegic refraction, angle of deviation, presence of amblyopia, change of refractive error during the follow-up period. Each finding were analyzed according to initial angle of deviation Results: At initial visit, mean angle of esodeviation was 25.42 PD (10∼60 PD) at distance and 26.23 PD (10∼ 60 PD) at near fixation. Of 52 patients, 13 (25%) patients had esodeviation of <20 PD, 30 (57.7%) had ≥20 PD and <40 PD and 9 (17.3%) had ≥40 PD. The mean age at onset was 30.26 months (birth∼7.5 years). Age at onset was 48.00 months in patients with esodeviation of <20 PD, 27.45 months in ≥20 PD and <40 PD, 15.89 months in ≥40 PD. The more the angle of deviation increased, the earlier the refractive accommodative esotropia developed. After the mean follow-up period of 41.4 months, reduction of hyperopia was 0.47 D per year. There was no significant difference of annual reduction of hyperopia according to angle of deviation. Conclusions: The more the angle of deviation increased, the earlier the refractive accommodative esotropia developed. It might be considered in diagnosis and treating refractive accommodative esotropia.

굴절교정수술로 유발된 부등시와 양안시기능의 상관관계

배소현,이가영,김희영,최동규,So Hyun Bae,Kayoung Yi,Hee Young Kim,Dong Gyu Choi 대한안과학회 2008 대한안과학회지 Vol.49 No.12

Purpose: To evaluate the effects of induced anisometropia by refractive surgery on binocular vision and to determine the usefulness of screening tools. Case summary: Anisometropia was induced in 7 patients by photorefractive keratectomy (PRK). Binocular vision was determined with the Titmus stereo test, Worth 4-dot test, 4 prism diopter base-out test and Bagolini striated lens test. Each test was performed before surgery, after PRK of one eye and after PRK of both eyes. After PRK of one eye, mean stereopsis by the Titmus stereo test was 1877.14 seconds of arc and after surgery of both eyes, 40 seconds of arc. After surgery of one eye, 6 patients (86.7%) showed suppression with the Worth 4 dot test at distance fixation and one patient (14.3%) at near fixation. After surgery of both eyes, all patients showed fusion with the Worth 4 dot test. After surgery of one eye, 3 patients (42.9%) showed suppression with the 4 prism diopter base-out test and after surgery of both eyes, 2 of the patients showed fusion. All patients showed fusion with the Bagolini striated lens test after surgery of one or both eyes. Conclusions: Most cases showed decreased binocular vision by the Titmus stereo test at near fixation and the Worth 4 dot test at distance fixation. The changes of binocular vision determined by each tool were variable. Therefore, improved results in evaluating binocular vision will be obtained with a combination of various tools including the Bagolini striated lens test and 4 prism base-out test.

중심장액맥락망막병증 환자의 이환된 눈과 무증상 반대편 눈, 정상 대조군과의 맥락막두께 비교

조준희,배소현,한재룡,남우호,김하경,Joon Hee Cho,So Hyun Bae,Jae Ryong Han,Woo Ho Nam,Ha Kyoung Kim 대한안과학회 2012 대한안과학회지 Vol.53 No.1

Purpose: To compare the choroidal thickness in central serous chorioretiopathy (CSC) patients and normal controls using spectral domain optical coherence tomography (SD-OCT). Methods: The authors compared the choroidal thickness in eyes with CSC, fellow eyes and in normal eyes. In addition, the authors attempted to determine any correlation between choroidal thickness and other factors such as age, height of serous retinal detachment, and spherical equivalent. Choroidal thickness was measured using a perpendicular line from the outer margin of the subfoveal retinal pigment epithelium to the inner surface of the sclera. Results: Twenty-five eyes of 25 CSC patients, 17 fellow eyes and 29 age-matched normal eyes were examined and categorized as group 1, group 2 and group 3, respectively. Subfoveal choroidal thickness was 370.64 ± 58.06 μm in group 1, 301.85 ± 47.83 μm in group 2, and 261.84 ± 48.22 μm in group 3. The choroidal thickness in group 1 was significantly greater than those in group 2 and group 3, and the choroidal thickness in group 2 was significantly greater than that in group 3 (p = 0.001, p < 0.001, p = 0.004, respectively), where the choroidal thickness showed a negative correlation with age (p = 0.015). Conclusions: The choroidal thickness was greater in eyes with CSC and in their fellow eyes compared to that in normal eyes. The results suggest that CSC may be caused by choroidal vascular hyperpermeability and increased hydrostatic pressure in the choroid. J Korean Ophthalmol Soc 2012;53(1):87-93

광주광역시 요양시설 코로나19 집단 발생 사례에서 코로나19 백신 접종에 의한 사망 예방 효과

류소연(So Yeon Ryu),조준휘(Jun Hwi Cho),이란(Ran Lee),박소영(So Yeong Park),정다운(Daun Jung),배소현(So Hyun Bae),고화평(HwaPyeong Ko) 한국농촌의학 지역보건학회 2022 농촌의학·지역보건 Vol.47 No.2

Objectives: The purpose of this study was to identify the effectiveness of COVID-19 vaccinations on deaths of the COVID-19 cases in some elderly long-term care facilities (LTCF) in Gwangju Metropolitan City. Methods: The study subjects were 659 COVID-19 cases in residents of 7 elderly LTCF, Gwangju, from 21 Dec. 2021 to 28 Jan. 2022. The used variables were confirmed dates for COVID-19, demographic characteristics, co-morbidities, presentation of symptoms, death and vaccination related characteristics including type of vaccine, doses and dates. Vaccination status were classified as not vaccinated, partially and fully vaccinated. The associations of vaccination status and deaths of COVID-19 cases were tested by chi-square test and multiple logistic regression analysis. Results: The rates of not vaccinated, partially vaccinated and fully vaccinated were 19.4%, 10.2% and 70.4%, respectively. The mortality rate of the cases was 6.4%. The death rate by vaccination status was 16.4% in not vaccinated, 15.4% in partially vaccinated and 2.6% in fully vaccinated, showing a statistically significant difference. The adjusted odds ratios (ORs) (95% confidence intervals, CIs) of death compared to fully vaccinated were 7.64 (3.87-16.34) in non-vaccinated, and 6.97 (3.44-14.14) in partially vaccinated. Conclusions: COVID-19 vaccination seems to work effectively in preventing deaths of COVID-19 cases of elderly LTCF residents. This finding support that fully vaccinations in high risk group such as elderly LTCF residents is very important for one of strategies of COVID-19 management.

맥락막신생혈관을 동반한 삼출성 나이관련황반변성 환자에서 광역학치료 후 10년간의 경과관찰

조두영,배소현,한재룡,김하경,남우호.Doo Young Cho. MD. So Hyun Bae. MD. Jae Ryong Han. MD. Ha Kyoung Kim. MD. PhD. Woo Ho Nam. MD 대한안과학회 2012 대한안과학회지 Vol.53 No.1

Purpose: To analyze the long-term results of photodynamic therapy (PDT) for exudative age-related macular degeneration (AMD). Methods: The clinical data of patients treated with photodynamic therapy for exudative age-related macular degeneration between April 2000 and December 2000 were analyzed. Patients were followed-up for at least 10 years after PDT. Results: Twenty-nine eyes of 29 patients were enrolled. Mean visual acuity on the logarithm of the minimum angle of resolution (log MAR) scale was 0.78 ± 0.34 at baseline, 1.01 ± 0.42 at 60 months, and 1.02 ± 0.41 at 120 months. Predominantly classic, minimally classic, and occult without classic choroidal neovascularization was noted in 51.7%, 17.2%, and 31.1% of patients, respectively. Visual acuity was improved by 1 or more lines in 27.6% of patients and was unchanged in 20.7% of patients, while 51.7% of patients had lost 1 or more lines of visual acuity by 120 months. Baseline visual acuity and age were associated with the final visual prognosis (p < 0.05). Four patients developed neovascular AMD in the contralateral eye. Conclusions: PDT is safe and effective for neovascular AMD. However, AMD can recur at any time and thus patients should be followed-up for a long period of time. J Korean Ophthalmol Soc 2012;53(1):59-67

다양한 원인에 의한 황반하 출혈에서 유리체강내 가스 주입술 여부에 따른 임상양상의 비교

김동욱,풍계현,배소현,김하경,남우호,Dong Wook Kim,Chi Shian Feng,So Hyun Bae,Ha Kyoung Kim,Woo Ho Nam 대한안과학회 2012 대한안과학회지 Vol.53 No.11

Purpose: To assess the clinical course of submacular hemorrhage (SMH) due to various chorioretinal diseases with or without pneumatic displacement and the factors related with the final visual outcome. Methods: The authors of the present study retrospectively reviewed the charts of 12 eyes (group 1) which underwent pneumatic displacement for SMH and the charts of 14 eyes (group 2) which did not receive pneumatic displacement. Best-corrected visual acuity (BCVA) at baseline was compared with the BCVA at 1, 3, and 6 months and on the final visit. Association between final BCVA and other clinical features was analyzed including age, baseline BCVA, duration of symptoms, and size of SMH. Results: In group 1, log MAR BCVA was 1.22 ± 0.66 at baseline and there was a significant BCVA improvement of 0.77 ± 0.57 at 6 months compared with baseline (p = 0.045). On the final visit, 6 eyes (50%) had gained 2 Snellen lines or more. In group 2, BCVA was significantly improved from 1.29 ± 0.70 at baseline to 1.06 ± 0.84 at 1 month (p = 0.045). Ten eyes (71.4%) had gained 2 Snellen lines or more on the final visit. In group 1, there were no factors correlated with final BCVA (p > 0.05), while the final BCVA was significantly correlated with age and baseline BCVA in group 2. Conclusions: Clinicians may expect conservative treatment to lead to significant improvement of BCVA in patients with SMH due to various chorioretinal diseases who did not undergo any procedures to displace the hemorrhage.

초기 성인기를 위한 동화 전시 공간 프로젝트 레빗홀 [RABBIT-HOLE] 제안

김준희 ( Joon-hee Kim ),배소현 ( So-hyun Bae ) 숙명여자대학교 디자인연구소 2020 숙명디자인학 연구 Vol.29 No.-

코로나19로 인해 생명의 위협을 받고 있는 2020년은 모두에게 어려운 시간이다. 코로나는 경제적 문제 뿐 아니라 사람들의 마음도 병들게 했다. 해당 논문은 그 중, 이제 막 성인이 된 이들이 겪는 코로나 블루를 통해 그들이 기존에 가지고 있던 스트레스의 원인과 해결책을 동화 전시를 통해 풀어볼 것이다. 2020 is a hard time for everyone, as Corona 19 is threatening their lives. Corona sickened people's minds as well as economic problems. Among them, the paper will solve the causes and solutions of their existing stress through a fairy tale exhibition through Corona Blue, which is experienced by those who have just become adults.

안과 전공의가 시행한 유리체내 주입술의 단기간 안전성 고찰

배영환(Young Hwan Bae),김하경(Ha Kyoung Kim),배소현(So Hyun Bae),윤창기(Chang Ki Yoon),마대중(Dae Joong Ma) 대한안과학회 2021 대한안과학회지 Vol.62 No.11

목적: 안과 전공의가 시행한 유리체내 주입술의 단기 안정성을 확인해보고자 한다. 대상과 방법: 2018년 1월부터 2020년 10월까지 본원에서 안과 전공의 및 망막 전문의에게 베바시주맙, 라니비주맙, 애플리버셉트 및 트리암시놀론 아세토나이드 유리체내 주입술을 처음으로 시행 받은 503명의 503안의 의무기록을 후향적으로 분석하였다. 결과: 총 503안 중 안과 전공의가 211안(전공의군), 망막 전문의가 292안(망막 전문의군)에 유리체내 주입술을 시행하였다. 두 군 간의 주사 전 임상양상의 차이는 원인 질환의 빈도를 제외하고 관찰되지 않았다. 안압상승은 전공의군에서 2안(0.95%), 망막 전문의군에서 5안(1.71%) 발생하였으나, 두 군 간의 유의한 차이는 없었다. 결막하 출혈은 전공의군에서 29안(13.74%), 망막 전문의군에서 32안(10.96%) 발생하였으나, 두 군 간의 유의한 차이는 없었다. 비감염성 안내염은 전공의군에서는 발생하지 않았고, 망막 전문의군에서 2안(0.68%) 발생하였으나, 두 군 간의 유의한 차이는 없었다. 감염성 안내염은 전공의군에서 2안(0.95%), 망막 전문의군에서 1안(0.34%) 발생하였으나, 두 군 간의 유의한 차이는 없었다. 두 군 모두에서 각막미란, 외상성 수정체 손상, 유리체출혈, 망막열공 및망막박리는 발생하지 않았다. 결론: 단기간의 안구 합병증의 발생 빈도를 평가하였을 때, 안과 전공의가 유리체내 주입술을 시행하여도 안전할 것으로 생각된다. Purpose: This study evaluated the short-term safety of resident-performed intravitreal injections. Methods: We retrospectively reviewed the medical records of 503 patients (503 eyes) treated for the first time in our hospital from January 2018 to October 2020 via intravitreal bevacizumab, ranibizumab, aflibercept, or triamcinolone acetonide injections by residents or retina specialists. In terms of short-term ophthalmic complications, patients were followed-up 1 day, 1 week, and 1 month after injection. Results: A total of 503 eyes of 503 patients were included. Intravitreal injections were given to 211 and 292 eyes by residents (the resident group) and retina specialists (the retina specialist group), respectively. There were no between-group differences in baseline characteristics except in terms of the indications for injection. Intraocular pressure elevation >5 mmHg occurred in two eyes (0.95%) in the resident group and five (1.71%) in the retina specialist group, but the difference was not statistically significant. Subconjunctival hemorrhage occurred in 29 eyes (13.74%) of the resident group and 32 eyes (10.96%) of the retina specialist group; again, the difference was not statistically significant. No case of noninfectious endophthalmitis occurred in the resident group but two (0.68%) cases occurred in the retina specialist group; again, the difference was not significant. There were two (0.95%) cases of infectious endophthalmitis in the resident group and one (0.34%) case in the retina specialist group; again, the difference was not significant. No corneal erosion, traumatic lens damage, vitreous hemorrhage, or retinal tearing or detachment were noted in either group. Conclusions: Resident-performed intravitreal injections appear to be safe.

벌쏘임에 의해 발생한 횡문근융해증 및 당뇨병케톤산증 1예

신승환 ( Seung Hwan Shin ),배소현 ( So Hyun Bae ),정혜진 ( Hye Jin Jung ),이상아 ( Sang Ah Lee ),김신우 ( Shin-woo Kim ),장현하 ( Hyun-ha Chang ),박한기 ( Han-ki Park ),이종명 ( Jong-myung Lee ),김수정 ( Sujeong Kim ) 대한천식알레르기학회(구 대한알레르기학회) 2018 Allergy Asthma & Respiratory Disease Vol.6 No.2

Bee stings result in diverse clinical manifestations from localized pain, rash to life-threatening systemic allergic reactions or toxic reactions. Toxic reactions include skin necrosis, pancreatitis, acute renal failure, hemolysis or coagulopathy, while systemic allergic reactions present with IgE-mediated anaphylaxis. We experienced a 63-year-old woman who developed rhabdomyolysis and diabetic ketoacidosis after bee sting. The patient was accompanied by pulmonary edema due to acute kidney injury, which was recovered by intensive hemodialysis treatment. Here, we report a rare and serious case induced by bee sting with a review of the literature. (Allergy Asthma Respir Dis 2018;6:128-130)