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Microcystis ( Cyanobacteria ) 분리주에서의 Microcystin 생산에 관한 연구
박혜경,진익렬,류홍일,류재근,도삼유평 (稻森悠平) ( Hae Kyung Park,Ing Nyol Jin,Hong Il Rhu,Jae Keun Ryu,Yuhei Inamori ) 한국물환경학회 1996 한국물환경학회지 Vol.12 No.1
Microcystis strains were isolated from water-blooms that had been collected from Korean lakes and reservoirs and cultured under uni-algal, non-axenic condition. Late exponential phase cells were harvested, concentrated and lyophilized for toxin extraction. Isolation, purification and qualitative and quantitative characterization of toxins were carried out by High Performance Liquid Chromatography(HPLC) system. Among 16 tested Microcystis strains, 14 strains produced hepatotoxic heptapeptide microcystins. The amounts and types of toxins were different according to strains. The identified toxins were microcystin-RR, -LR and YR.
생물 2 교과서 광합성 단원의 오류 분석 및 생물 교사의 오류 인지 조사
박혜경 ( Hae Kyung Park ),윤기순 ( Ki Soon Yoon ),권덕기 ( Duck Kee Kwon ) 경북대학교 과학교육연구소 2008 科學敎育硏究誌 Vol.32 No.1
본 연구의 목적은 제7차 교육과정 생물Ⅱ 교과서의 `광합성` 단원에 나타난 내용 오류를 분석하여 수정 예시를 제시함과 아울러 이 오류가 과목 담당 교사들에 의해 어느 정도 지적되고 수정되는지 조사함으로써 오류가 수정 지도될 가능성을 알아보는데 있다. 이를 위해, 제7차 교육과정 생물Ⅱ 8종 교과서 광합성 단원에서 오류를 분석하고, 오류가 담긴 교과서 내 지문으로 설문문항을 개발하여 교사들의 오류 인지 및 수정정도를 조사하였다. 8종의 교과서 광합성 단원의 13개 학습주제에서 발견된 오류의 수는 총 48개였으며, 1종의 교과서에서 1개의 학습주제에 대해 4가지 오류가 나타나거나 동일한 오류가 여러 교과서에서 각각 나타나기도 하였다. 35명의 생물Ⅱ과목 담당 교사를 대상으로 한 설문 조사결과, 교과서에 나타난 그래프(문항 6번), 잘못된 용어(문항 4-3번), 잘못된 그림(문항 1-2번) 등과 같은 단순오류의 경우를 오류를 정확히 지적하고 수정한 예가 각각 43%, 40%, 32%로 비율이 높았다. 그러나 광합성의 명반응 과정을 설명하는 삽화에서 에너지 준위의 개념이 접목되어야 하는 오류에 대한 지적을 한 사례는 없었다. 이러한 결과는 생물Ⅱ 광합성 단원에 나타나는 오류는 정확히 수정되어 지도될 가능성이 낮음을 시사한다. 학생들이 광합성에 대한 오개념을 가질 가능성을 낮추려면 오류를 수정 사항에 대한 자료를 교사들에게 배부하거나 교사연수 또는 워크샵 등을 통해 이에 대한 정보를 나눌 기회가 제공되어야 할 것이다. The purpose of this study was to know whether or not any wrongful description or simple errors were in photosynthesis unit of Biology Ⅱ textbook under 7th national curriculum and if so, to know whether or not high school teachers recognized and corrected properly the mistakes. The mistakes in photosynthesis unit of text books were determined by the comparison with several reference books and through examination by three plant physiologists in 8 different Biology Ⅱ textbooks. After the mistakes were analysed, the survey using contents of textbook containing the mistakes was conducted on high school teachers teaching Biology Ⅱ. As a result, 48 mistakes were determined in 13 subjects. As many as four mistakes were found even in one subject in a certain textbook and a same mistake was found repeatedly in several textbooks. The survey result showed that the teachers who pointed exactly the mistakes out corrected properly, however, the percentage of these ones out of 35 teachers replied to survey was less than 50%. The ratios of correction out of total number of responses were high in question #6 (43%), #4-3 (40%), and #1-2 (32%) which were containing a simple mistake in graph, a wrong word and a wrong picture, respectively. But, no one pointed out and made correction in question #5-1 and #5-2 which were containing Z scheme of light reaction without the legend of vertical axis that should be explained as electron energy or standard reduction potential. The result indicates the possibility that the mistakes in photosynthesis unit of Biology Ⅱ textbook can be corrected and teached properly by teachers may be low. In order to reduce the possibility that students may have misconceptions about photosynthesis, the list of print`s errors should be provided to the teachers and/or the training program and/or workshop for in-service high school biology teachers was recommended.
한국산 유독 남조류의 독소함량을 근거로 한 조류경보제 발령기준 검토
박혜경 ( Hae Kyung Park ),김화빈 ( Hwa Bin Kim ),이재정 ( Jay J. Lee ),이재안 ( Jae An Lee ),이혜진 ( Hae Jin Lee ),박종환 ( Jong Hwan Park ),서정관 ( Jung Kwan Seo ),윤석제 ( Seok Jea Youn ),문정숙 ( Jeong Suk Moon ) 한국물환경학회 2011 한국물환경학회지 Vol.27 No.4
We investigated the ranges of total cellular microcystins content of cyanobacterial blooms collected in Korean lakes and rivers from 2005 to 2009. The amount and composition of microcystins of Korean cyanobacteria varied depending on the sampling water bodies and dominant cyanobacterial genera. Toxic cyanobacterial cell numbers equivalent to 1 μg MCYSTs/L using total cellular microcystin content of Korean cyanobacteria were in the range of 2,348 to 66,980,638 cells/mL. Only four samples among forty nine samples showed less cell numbers than current criterion of Harmful Algae Alert System, 5,000 cells/mL indicating current criterion do not reflect properly the microcystins content of Korean cyanobacteria. Anabaena and Aphanizomenon spp. showed three to six times higher cell numbers equivalent to 1 μg MCYSTs/L than Microcystis spp. To propose criteria of Harmful Algae Alert System for Korean toxic cyanobacteria, we calculated about 50% selective geometrical means of cyanobacterial cell numbers equivalent to 1 μg MCYSTs/L in order of toxic content. The proposed criteria for Microcystis, Oscillatoria, Anabaena, and Aphanizomenon spp., are 10,000, 20,000, 40,000, and 80,000 cells/mL, respectively.