최대환기능의 간접측정법에 관한 연구

박해근,김광진,성혜숙,전병숙,Park, Hae-Kun,Kim, Kwang-Jin,Sung, Hae-Sook,Jeon, Byung-Sook 대한생리학회 1977 대한생리학회지 Vol.11 No.2

The maximum voluntary ventilation (MVV) is one of the most widely used pulmonary function test, but its measuring method was very difficult and unreliable. However, it is need to get more easy and simple measuring method of MVV. Therefore, this study was attempted to get more easy and simple measuring method of MVV by means of the forced expiratory volume $(FEV_{T})$. The young and healthy 1,000 Korean students(592 male and 408 female) were cheesed for this purpose and whose ages were from 8 to 20 years. A spirometer (9L, Collins Co.) was used for the MVV and FEV, and they were measured 3 times at standing position, and the highest value was used. In the measurements, the subjects for MVV were asked for the breath as fast and deeply as possible for 12 seconds, and for FEV were asked for the rapid and forceful exhalation after a maximal inhalation (forced expiratory curve). In the FEV measurements toward the end of the expiration, the subjects were exhaused to continue the effort until no further gas was expired. During these measurements, the investigator stood by the subject to give a constant encouragement. FEV were calculated in the volume exhaled during the one-half $(FEV_{0{\cdot}5,}\;ml)$, the first second $(FEV_{1{\cdot}0,}\;ml)$ and the percentage of the total vital capacity exhaled during the one-half second $(FEV_{0{\cdot}5,}\;%)$. The results are summarized as follows: 1) The values of MVV were increased linearly with ages until 20 in both sexes. The values of male at the age of 20 was $168.2{\pm}2.5L/min$, and female at the age of 17 was $112.3{\pm}3.0L/min$, respectively. 2) The values of FEV (ml) were increased linearly with ages until 20 in both sexes. The values of $FEV_{0{\cdot}5}$ were $2,797{\pm}65.7ml$ in the male of 20 years and were $2,088{\pm}54.6ml$ in the female of 17 years, and of $FEV_{1{\cdot}0$ were $4,119{\pm}68.2ml$ in the male of 20 years and were $2,897{\pm}65.9ml$ in the female of 17 years, respectively. 3) The correlation coefficients between MVV and $FEV_{0{\cdot}5}\;or\;FEV_{1{\cdot}0$ (ml) were 0.82 or 0.85 in the male, and 0.77 or 0.79 in the female, respectively. 4) The prediction formulae for MVV to be derived from above results were: For male: MVV (L/min) =7.19+$0.05{\times}FEV_{0\cdot5}(ml)$, MVV (L/min)=11.25+$0.04{\times}FEV_{1\cdot0}(ml)$ For female: MVV (L/min)=16.03+$0.05{\times}FEV_{0\cdot5}(ml)$, MVV (L/min)=9.47+$0.03{\times}FEV_{1\cdot0}(ml)$.

한국 어린이 및 청소년의 폐환기능에 관한 연구 - 특히 표준치 예측 수식에 관하여 -

박해근(Park, Hae-Kun),김광진(Kim, Kwang-Jin) 대한생리학회 1975 대한생리학회지 Vol.9 No.2

The maximum breathing capacity (MBC) and the maximum mid-expiratory flow rate (MMF) are widely used in evaluation of the ventilatory function, among various parameters of pulmonary function. The MBC volume is the amount of gas which can be exchanged per unit time during maximal voluntary hyperventilation. Performance of this test, unlike that of single breath maneuvers, is affected by the integrity of the respiratory bellows as a whole including such factors are respiratory muscle blood supply, fatigue, and progressive trapping of air. Because of this, the MBC and its relation to ventilatory requirement correlates more closely with subjective dyspnea than does any other test. The MMF is the average flow rate during expiration of the middle 50% of the vital capacity. The MMF is a measurement of a fast vital capacity related to the time required for the maneuver and the MMF relates much better to other dynamic tests of ventilatory function and to dyspnea than total vital capacity, because the MMF reflects the effective volume, or gas per unit of time. Therefore, it is important to have a prediction formula with one can compute the normal value for the subject and the compare with the measured value. However, the formulas for prediction of both MBC and MMF of the Korean children and adolescents are not yet available in the present. Hence, present investigation was attempt to derive the formulas for prediction of both MBC and MMF of the Korean children and adolescents. MBC and MMF were measured in 1,037 healthy Korean children and adolescents (1,035 male and 1,002 female) whose ages ranged from 8 to 18 years. A spirometer (9L, Collins) was used for the measurement of MBC and MMF. Both MBC and MMF were measured 3times in a standing position and the highest values were used. For measurement, the CO₂ absorber and sadd valve were removed from the spirometer in order to reduce the resistance in the breathing circuit and the subject was asked to breathe as fast and deeply as possible for 12 seconds in MBC and to exhale completely as fast as possible after maximum inspiration for MMF. During the measurement, investigator stood by the subject to give a constant encouragement. All the measured values were subsequently converted to values at BTPS. The formulas for MBC and MMF were derived by a manner similar to those for Baldwin et al (1949) and Im (1965) as function of age and BSA or age and height. The prediction formulas for MBC (L/min, BTPS) and MMF (L/min, BTPS) of the Korean children and adolescents as derived in this investigation are as follows: For male, MBC=[41.70+{2.69 X Age(years)}] X BSA (m²) MBC=[0.083+{0.045 X Age(years)}] X Ht (cm) For female, MBC=[45.53+{1.55 X Age(years)}] X BSA (m²) MBC=[0.189+{0.029 X Age(years)}] X Ht (cm) For male, MMF= [0.544+{0.066 X Age(years)}] X Ht (cm) For female, MMF=[0.416+{0.064 X Age(years)}] X Ht (cm)

인삼(人蔘)이 흰 생쥐의 운동능력(運動能力) 및 유산생성량(乳酸生成量)에 미치는 영향(影響)

홍성일,박해근,Hong, Sung-Il,Park, Hae-Kun 대한생리학회 1975 대한생리학회지 Vol.9 No.1

This experiment was carried out to observe a biological effect of ginseng on the weight gain, physical performance and lactic acid production after exercise in mice. A group of mice weighing about 19 gm was divided into ginseng and cotrol group. on the treadmill (Exp. I & II) and LDH activity of liver and heart homogenates (Exp. II) were determined. Results are summerized as follows; 1. Body weight gain was greater id ginseng group than in control and the difference was statistically significant at 9th and 16th days of experimental period. 2. Maximal running time of ginseng was found to be longer than that of control (p<0.05) in experiment I and the experiment II also revealed the significant increase in maximal running time in ginseng group. 3. Bloo lactate concentration of 48 hour-rest from physical exertion was lower in ginseng group than in control (p<0.05). 4. LDH activity in liver homogenate was lower compared to control group, but in heart homogenate, it was greater in ginseng group. Above findings may be concluded tat the range of biological dose (20 mg/mice/day) of ginseng powder stimulated the body weight gain and increase of physical performance and its mechanism might be attributable to a lower level of blood lactic acid. The adaptive change of LDH activity also contributed to the change in lactate level in blood and tissue.

폐환기능 측정기기에 따른 성적차이에 관한 연구

김대하 ( Dae Ha Kim ),박해근 ( Hae Kun Park ),성혜숙 ( Hae Sook Sung ),윤석주 ( Seok Joo Yoon ),이태훈 ( Tae Hoon Lee ),황정운 ( Jeong Woon Whang ) 대한내과학회 1984 대한내과학회지 Vol.27 No.12

Prostaglandin $F_{2{\alpha}}$가 가토 대동맥 평활근 수축성에 미치는 영향

정수성,김세훈,장석종,박해근,Chung, Soo-Sung,Kim, Se-Hoon,Chang, Seok-Jong,Park, Hae-Kun 대한생리학회 1989 대한생리학회지 Vol.23 No.1

The effects of prostaglandin $(PGF_{2{\alpha}})$ on the contractility of vascular smooth muscle were investigated in the helical strip of the rabbit aorta. The aortic strip was immersed in the phosphate-buffered Tyrode's solution which was equilibrated with 100% $O_{2}$ at $35^{\circ}C$ and its isometric tension was measured. The contraction was induced by $(PGF_{2{\alpha}})$, norepinephrine (NE), or potassium (40 mM) in the nomal Tyrode's solution (1 mM, $Ca^{2+}$) or $Ca^{2+}-free$ Tyrode's solution. Effects of verapamil and phentolamine on the contraction were also observed. The aortic strip began to contract at the concentration of $5\;{\mu}g%$ and reached the maximal contraction at the concentration of $150\;{\mu}g%$ $(PGF_{2{\alpha}})$. The maximal contraction was corresponded respectively to $52.2{\pm}3.0%$ and $81.5{\pm}3.5%$ of maximal contraction by NE $(1{\times}10^{-5}M)$ and 40 mM $K^{+}$. And the maximal contractions by $(PGF_{2{\alpha}})$ or NE were induced at the concentration of about 1 mM $Ca^{2+}$. $(PGF_{2{\alpha}})$ induced the contraction of aortic strip even after induction of contraction by 40 mM $K^{+}$ and the contraction by $(PGF_{2{\alpha}})$ was not blocked by the ${\alpha}-receptor$ blocker, phentolamine. And the contraction by the $(PGF_{2{\alpha}})$ was inhibited partially by a verapamil at the concentration of $1{\times}10^{-5}M$ and the contraction began to increase at the concentration of $1{\times}10^{-4}M$ verapamil. Whereas the contraction by NE was completely blocked by verapamil. Though both the $(PGF_{2{\alpha}})$ and NE induced the contraction in the $Ca^{2+}-free$ Tyrode's solution, the peak tension was not maintained. But the rate of tension decline was lower in the contraction by $(PGF_{2{\alpha}})$ than in that by NE. The verapamil did not inhibit the contraction by $(PGF_{2{\alpha}})$ in the $Ca^{2+}-free$ Tyrode's solution and increased the contraction at the concentration of above $1{\times}10^{-4}M$. The NE-induced contraction in the $Ca^{2+}-free$ Tyrode's solution was inhibited completely by a verapamil. From the above results it is suggested that the contraction induced by $(PGF_{2{\alpha}})$ results from the promotion of the both $Ca^{2+}$ influx and the intracellular $Ca^{2+}$ release by different way from NE.

수소이온농도 변화의 수축물질에 따른 가토신동맥 수축에 미치는 영향과 기전

장석종,김세훈,전병화,박해근,Chang, Seok-Jong,Kim, Se-Hoon,Jeon, Byeong-Hwa,Park, Hae-Kun 대한생리학회 1990 대한생리학회지 Vol.24 No.1

The effects of $H^{+}$ on the arterial contraction and their mechanisms were investigated in the renal artery of a rabbit. The helical strips of isolated renal artery were immersed in the HEPES-buffered or $CO_{2}/HCO_{3}^{-}$-buffered Tyrode's solution. The contractions induced by agonists (norepinephrine, histamine, serotonin and angiotensin II) or high $K^{+}$ were observed with change of extracellular or intracellular $H^{+}$ concentration. The contractions induced by norepinephrine, histamine, serotonin, angiotensin II or high $K^{+}$ in HEPES-buffered Tyrode's solution were inhibited by increase in extracellular $H^{+}$ concentration and potentiated by decrease in extracellular $H^{+}$ concentration. The degrees of these effects were most evident in the contraction induced by serotonin and angiotensin II, moderate in those by histamine and high $K^{+}$, and least in those by norepinephrine. Maximal contraction by norepinephrine, histamine and high $K^{+}$ were not influenced by change in extracellular $H^{+}$ concentration, but influenced in those contration by serotonin and angiotensin II. The attenuated contractions by an acidic pH were not returned to the level of contraction at normal pH (7.4) by elevation of extracellular $Ca{2+}$ concentration. The agonists (norepinephrine, histamine and serotonin)-induced contractions in $Ca{2+}$-free Tyrode's solution were also attenuated by increase in extracellular $H^{+}$ concentration and potentiated by decrease in extracellular $H^{+}$ concentration. Elevation of $Pco_{2}$ in the $CO_{2}/HCO_{3}^{-}$-buffered Tyrode's solution, which increase the intracellular $H^{+}$ concentration, at constant extracellular pH (7.4), increased the contraction by 30 mM $K^{+}$. From the above results, it is suggested that the decrease in contractions by increase in extracellular $H^{+}$ concentration may be resulted from that $H^{+}$ make the receptors less sensitive to agonists and cell membrane hyperpolarize and then inhibit the $Ca{2+}$ influx as well as $Ca{2+}$ release from intracellular $Ca{2+}$ storage site.

가토 신동맥의 고농도 Histamine에 의한 노아드레날린 유발 수축 및 K-경축 약화 기전

이성우(Lee, Sung-Woo),김세훈(Kim, Se-Hoon),장석종(Chang, Seok-Jong),박해근(Park, Hae-Kun) 대한생리학회 1989 대한생리학회지 Vol.23 No.2

The contraction of renal arterial strip by no.epineph.me (NE) or 40 mM K⁺ were Significantly attenuated after histamine (10^-5 M)-induced contraction. The mechanisms of this phenomenon were investigated in the helical strips of isolated renal artery with the measurement of isometric tension. The arterial strip was immersed in the tris-buffered Tyrode s solution which was equilibrated with 100% O₂ at 35℃. The contraction was induced by NE or 40 mM K⁺ during the recovery from the histamine-induced contraction which lasted for 15 minutes. The contraction by NE was also attenuated in the Ca²⁺-free Tyrode s solution and the increase of contraction by addition of 2 mM Ca²⁺ was attenuated as well. This attenuation phenomenon was not observed in the presence of low concentration (3 X 10^-7 M) of histamine. This attenuation was not affected by destruction of endothelium, pretreatment with papaverine or propranolol. This attenuation was partially inhibited by pretreatment of ouabain or in low K⁺(0.5 mM) Tyrode s solution. But the attenuation in the Ca²⁺-free Tyrode s solution was not inhibited. Furthermore this attenuation was completely blocked by pretreatment of djphenhydramine (H₁-receptor blocker) and potentiated by pretreatment of cimetidine (H₂-receptor blocker). This attenuation Phenomenon was disappeared after recovery of 1 hour. From the above results, it is suggested that the attenuation phenomenon may be resulted partially from the activation of Na⁺-K⁺ exchange pump and partially from the depletion of intracellular Ca²⁺ pool after the histamine-induced contraction mediated through H₁-receptor function.

흉곽수술을 받은 환자의 최대운동 능력에 관한 연구

김기호 ( Ki Ho Kim ),이원영 ( Won Young Lee ),김형덕 ( Hyung Duk Kim ),박해근 ( Hae Kun Park ),백광세 ( Kwang Se Paik ),홍석기 ( Suk Ki Hong ) 대한내과학회 1972 대한내과학회지 Vol.15 No.6

An Experiment was carried out as follows: a) Control group consisted of 20 young healthy individuals. b) The experiment group consisted of 42 patients who had thoracic surgery; lobectomy 18 cases, thoracoplasty 11 cases, and pneumonectomy 13 cases. c) Meth

Prostaglandin F_2α가 가토 대동맥 평활근 수축성에 미치는 영향

정수성(Chung, Soo-Sung),김세훈(Kim, Se-Hoon),장석종(Chang, Seok-Jong),박해근(Park, Hae-Kun) 대한생리학회 1989 대한생리학회지 Vol.23 No.1

The effects of prostaglandin (PGF_2α) on the contractility of vascular smooth muscle were investigated in the helical strip of the rabbit aorta. The aortic strip was immersed in the phosphate-buffered Tyrode s solution which was equilibrated with 100% O_{2} at 35℃ and its isometric tension was measured. The contraction was induced by (PGF_2α), norepinephrine (NE), or potassium (40 mM) in the nomal Tyrode s solution (1 mM, Ca²⁺) or Ca²⁺-free Tyrode s solution. Effects of verapamil and phentolamine on the contraction were also observed. The aortic strip began to contract at the concentration of 5 μg% and reached the maximal contraction at the concentration of 150 μg% (PGF_2α). The maximal contraction was corresponded respectively to 52.2±3.0% and 81.5±3.5% of maximal contraction by NE (1 X 10^-5M) and 40 mM K⁺. And the maximal contractions by (PGF_2α) or NE were induced at the concentration of about 1 mM Ca²⁺. (PGF_2α) induced the contraction of aortic strip even after induction of contraction by 40 mM K⁺ and the contraction by (PGF_2α) was not blocked by the α-receptor blocker, phentolamine. And the contraction by the (PGF_2α) was inhibited partially by a verapamil at the concentration of 1 X 10^-5M and the contraction began to increase at the concentration of 1 X 10^-4M verapamil. Whereas the contraction by NE was completely blocked by verapamil. Though both the (PGF_2α) and NE induced the contraction in the Ca²⁺-free Tyrode s solution, the peak tension was not maintained. But the rate of tension decline was lower in the contraction by (PGF_2α) than in that by NE. The verapamil did not inhibit the contraction by (PGF_2α) in the Ca²⁺-free Tyrode s solution and increased the contraction at the concentration of above 1 X 10^-4M. The NE-induced contraction in the Ca²⁺-free Tyrode s solution was inhibited completely by a verapamil. From the above results it is suggested that the contraction induced by (PGF_2α) results from the promotion of the both Ca²⁺ influx and the intracellular Ca²⁺ release by different way from NE.

선천성 심장질환에 있어서 색소 희석법에 의한 전류량의 측정

차홍도 ( Hong Do Cha ),서정삼 ( Chung Sam Suh ),박해근 ( Hae Kun Park ),김홍길 ( Hong Gil Kim ),홍필훈 ( Pill Whoon Hong ) 대한내과학회 1966 대한내과학회지 Vol.9 No.4