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초자화동결된 생쥐 Preantral Follicle의 체외성장과 배란
박지권,백원영,Park, Ji-Kwon,Paik, Won Young 대한생식의학회 2005 Clinical and Experimental Reproductive Medicine Vol.32 No.2
Objective: To define an appropriate vitrification condition of preantral follicle that yields high survival and to evaluate growth and ovulation rate of mouse follicles during in vitro culture after vitrification. Methods: Preantral follicles were isolated mechanically from mouse ovaries that were surgically recovered from mice aged 14 days. Retrieved preantral follicles were placed in EG (Ethylene Glycol) for 2, 5, 10 minutes and transferred to EFS-40 (40% EG, 18% Ficoll-70, 0.5 M sucrose) for 0.5, 1, 2 minutes. And then, preantral follicles were placed onto an EM grid and submerged immediately in liquid nitrogen. Thawing was carried out at room temperature. After defining the most appropriate vitrification condition that yields high survival, in vitro growth and ovulation rate of follicles were evaluated. Results: Appropriate vitrification condition that yield high survival rate ($83.2{\pm}2.1%$) of preantral follicle was EG for 5 minutes and EFS-40 for 0.5 minutes. In vitro survival rate of the vitrified preantral follicles were $85.5{\pm}0.5%$, $67.9{\pm}0.8%$ and $40.2{\pm}0.5%$ on day 2, 6 and 10. And in vitro growth of the vitrified preantral follicles were $107.1{\pm}16.1{\mu}m$, $117.1{\pm}18.4{\mu}m$, $178.4{\pm}45.6{\mu}m$ and $325.4{\pm}54.4{\mu}m$ on day 0, 2, 6 and 10. Although in vitro survival rate and growth of vitrified preantral follicles were lower than that of non-vitrified preantral follicles, the patterns of survival and growth were similar in vitrified and non-vitrified preantral follicles. The ovulation rate of antral follicles that was grown from vitrified preantral follicles was $32.6{\pm}1.2%$. Conclusion: Vitrified preantral follicles could be grown to antral sizes, and mature oocytes that can be used for IVF-ET programs were produced successfully. These data suggest that cryopreservation of preantral follicle by vitrification can be used for the preservation of the fertility.
박지권 ( Ji Kwon Park ),김선임 ( Seon Im Kim ),백원영 ( Won Young Paik ) 대한산부인과학회 2007 Obstetrics & Gynecology Science Vol.50 No.6
목적: 만성적인 저산소증과 관련된 자간전증이 합병된 임신의 태반에서 세포고사와 관련 있는 14-3-3 단백과 Bcl-2계 단백의 발현을 알아보고, 두 단백간의 상호작용을 보고자 하였다. 연구방법: 자간전증 임산부의 태반 5예와 정상 대조군 임산부의 태반 5예를 대상으로 Western blot 및 Immunoprecipitation related Western blotting을 시행하여 결과를 비교, 관찰하였다. 결과: Western blot에서 Bax와 14-3-3 zeta는 정상 대조군보다 자간전증 임산부의 태반에서 유의하게 증가된 소견을 보였다. Immunoprecipitation related Western blotting 에서 14-3-3 zeta와 Bax의 상호작용은 자간전증 임산부의 태반에서 유의하게 감소된 소견을 보였다. 결론: 정상 태반에 비해 자간전증 태반에서 Bax 발현의 유의한 증가는 태반에서의 세포고사 촉진인자로 Bax가 관여할 것으로 추측되며, 14-3-3 zeta와 Bax의 interaction의 유의한 감소는 자간전증 태반에서 활동성인 Bax의 양이 증가하여 세포고사를 유도하는 것으로 이해되어 진다. 향후 태반에서의 세포고사에서 14-3-3 단백으로부터 Bax를 해리시키는 기전에 관한 추가적인 연구가 필요하리라 사료된다. Objective: Purpose of our study was to examine the expression level of 14-3-3 proteins and Bcl-2 family and to estimate the interaction between 14-3-3 proteins and Bcl-2 family in normal and preeclamptic placenta. Methods: Placental tissues were sampled from preeclampsia with caesarean delivery (n=5) and normal pregnant women with caesarean delivery (n=5). Western blot and immunoprecipitation related Western blotting were performed on all placental samples. Unpaired Student t-test was used to determine the statistical significance. Results: Western blot analysis revealed that the expression of Bax and 14-3-3 zeta was significantly greater in the preeclamptic placenta than in the normal placenta. Immunoprecipitation related Western blotting revealed that the interaction between 14-3-3 zeta and Bax was significantly less in the preeclamptic placenta than in the normal placenta. Conclusion: Increased expression of Bax and reduced interaction (between) 14-3-3 zeta and Bax in preeclamptic placenta might influence pathogenesis or sequelae of preeclampsia. Further study is needed to identify the trigger that induces dissociation of Bax from 14-3-3 proteins.
조기진통 임신부의 자궁경부에서 마이코플라즈마 감염의 임상적 고찰
박지권 ( Ji Kwon Park ),신정규 ( Jeong Kyu Shin ),최원준 ( Won Jun Choi ),이순애 ( Soon Ae Lee ),이종학 ( Jong Hak Lee ),백원영 ( Won Young Paik ) 대한주산의학회 2005 Perinatology Vol.16 No.2
목적: 정상 임부와 조기진통 임부의 자궁경부에서 마이코플라즈마 감염의 임상적 특징과 조기분만 진통과의 상호연관성을 알아보고자 하였다. 방법 : 2002년 1월부터 2002년 8월까지 ○○대학교 병원에 내원한 임신 20~37주 사이의 조기진통 임부 35명과 정상 만삭임부 74명을 대상으로 하였다. 자궁경관 질 분비물을 채취하여 일반세균배양과 마이코플라즈마 배양검사를 동시에 시행하였다. 마이코플라즈마 검사는 U. urealyticum이 urea를 가수분해 하고 M. horminis가 arginine을 대사하는 성질을 이용한 Mycoscreen Kit(R)을 사용하였다. 결과: M. horminis의 감염이 조기진통임부 35명중 7명(20%), 정상임부 74명 중 2명(2.7%)에서 양성으로 조기진통임부에서 유의하게 높았다(p<0.01). U. urealyticum의 감염이 조기진통임부 25명(71.4%), 정상임부에서 43명(58.1%)로 차이가 있었으나 통계적으로 유의하지는 않았다(p>0.05). 다른 일반세균의 감염은 조기진통임부 11명(31.4%), 정상임부 8명(10.8%)로 조기진통임부에서 유의하게 높았다(p<0.05). 조기진통이나 조기양막파수를 진단한 후 분만까지 걸린 기간은 M. hominis 양성군의 경우 21.7일, M. hominis 음성군의 경우 3.5일로 유의한 차이가 있었다(p<0.05). 결론: M. hominis 감염과 일반세균의 감염은 조기진통 및 조기 양막파수의 위험을 증가시키는 것으로 볼 수 있으며, 조기 양막파수를 일으킨 임부에서 M. hominis 감염은 비감염 임부에 비하여 임신 지속 기간이 유의하게 길어 적절한 항균제 및 적극적인 임신유지요법이 임신기간 연장에 도움이 될 것으로 사료된다. Objective: To evaluate the infection rate of cervicovaginal U. urealyticum and M. hominis in normal full-term and preterm labor women, to know whether these infections are related to the increase of preterm labor incidence. Methods: We took the test for U. urealyticum and M. hominis in cervicovaginal fluid in 35 cases of premature labor women and 74 cases of normal full-term women. Culture of U. urealyticum and M. hominis with Mycoscreen kit(R) and other bacterial culture were performed, simultaneously. Clinical outcomes were reviewed and the test results between preterm labor and full-term pregnancy group were compared. Student t-tests and Chi-square, Fisher`s exact test were used to determine the statistical significance. Results: The infection rates of U. urealyticum in preterm labor group and normal full-term group were 71.4% (25/35 cases) and 58.1% (43/74 cases), respectively (p>0.05). The M. hominis infection rates in premature labor group and normal full-term group were 20% (7/35 cases) and 2.7% (2/74 cases), respectively (p<0.01). Other bacterial infection rates in premature labor and normal full-term group were 31.4% (11/35 cases) and 10.8% (8/74 cases), respectively (p<0.01). Among the premature labor women, the duration of pregnancy prolongation after onset of premature labor was significantly longer in M. hominis positive group than that in M. hominis negative group (21.7 days vs 3.5 days). Conclusion: The lower genital tract infection may be an important causative factor of premature labor, especially with colonization of M. hominis. It was suggested that the efforts of obstetricians to detect and control genital M. hominis and bacterial infection may, in some degree, prevent the preterm labor and birth.
박미화 ( Mi Hwa Park ),한유정 ( You Jung Han ),박영 ( Young Park ),백종철 ( Jong Chul Baek ),박지권 ( Ji Kwon Park ),이종학 ( Jong Hak Lee ),백원영 ( Won Young Paik ) 대한산부인과학회 2009 Obstetrics & Gynecology Science Vol.52 No.8
Primary malignant melanoma of the vagina is extremely rare, accounting for 3% of all primary malignant tumor of the vagina and 0.3~1% of all malignant melanomas in the female. The amelanotic melanoma of the vagina showing no melanin granules on histological examination is exceedingly rare, accounting for only about 10% of all melanoma of the vagina. The amelanotic melanoma of the vagina is often difficult to differentiate from non-epithelial malignant tumor because of the minimal number of melanin granules. We describe a case of primary amelanotic melanoma of the vagina, which was initially suspected to be a non-epithelial malignant tumor, especially malignant peripheral nerve sheath tumor (MPNST), but was correctly diagnosed by HMB-45 antibody and S-100 protein immunohistochemical staining. So we present this case with a brief review of literature.
Methotrexate와 Misoprostol을 이용한 보존적 치료로 성공한 감입태반
한유정 ( You Jung Han ),박미화 ( Mi Hwa Park ),조지혜 ( Ji Hye Cho ),백종철 ( Jong Chul Baek ),박지권 ( Ji Kwon Park ),이종학 ( Jong Hak Lee ),백원영 ( Won Young Paik ) 대한주산의학회 2009 Perinatology Vol.20 No.3
Placenta accreta is a rare complication of pregnancy with high rates of maternal morbidity and mortality. Although the incidence of placenta accreta was rare in the past, it is sharply rising as a result of the dramatic increases in the cesarean section rate. Hysterectomy is the most effective way to manage the placenta accreta in the majority of cases. While in many situations hysterectomy will remain appropriate, there are other management options available involving conservative approaches. In this article, we report our experience of a successful conservative treatment using methotrexate and misoprostol to a patient in whom the whole placenta was not detachable from the uterus due to placenta increta.
임신 제1삼분기에 진단된 배꼽탈장을 동반한 7번 세염색체증 모자이시즘
고민경 ( Min Kyung Ko ),조인애 ( In Ae Cho ),이재익 ( Jae Ik Lee ),박지권 ( Ji Kwon Park ),신정규 ( Jeong Kyu Shin ),최원준 ( Won Jun Choi ),이순애 ( Soon Ae Lee ),이종학 ( Jong Hak Lee ),백원영 ( Won Young Paik ) 대한산부인과학회 2012 Obstetrics & Gynecology Science Vol.55 No.10
The trisomy 7 is rare autosomal aneuploidy worldwide, which accounts for 4%-10% of all trisomies, and most of the cases are mosaicism. The partial trisomy 7 mosaicism demonstrates facial deformities, short neck, hypotonia, developmental delay, severe growth retardation, renal anomalies, cardiac defect, skeletal anomalies with a wide range of characteristics and severities. The cases of full trisomy 7 are even rarer and the characteristics are not known well. Recently we have experienced a case of full trisomy 7 mosaicism with omphalocele, cleft palate, lower set of ears, single eye, scoliosis, upper limb deformity. It was prenatally diagnosed by chorionic villi sampling based on abnormal ultrasonographic findings at 12 weeks of gestation, using conventional karyotyping and bacterial artificial chromosome array comparative genomic hybridization. We report this case with brief review of literature.