발생중인 생쥐 치아 및 치주조직에서 치주인대-특이 단백질; PDLs22의 발현

박중원,박병기,김상목,김병옥,박주철,Park, Jung-Won,Park, Byung-Ki,Kim, Sang-Mok,Kim, Byung-Ock,Park, Joo-Cheol 대한치주과학회 2002 Journal of Periodontal & Implant Science Vol.32 No.1

The periodontal ligament(PDL) is a unique tissue that is crucial for tooth function. However, little is known of the molecular mechanisms controlling PDL function. PDL-specific protein;PDLs22 had been previously identified as a novel protein isolated from cultured human PDL fibroblasts using subtraction hybridization between human gingival fibroblasts and PDL fibroblasts. The aim of this study was to examine the expression pattern and tissue localization of PDLs22 protein in embryonic and various postnatal stages of developing mouse using immunohistochemical staining. Embryos (E18) and postnatal (P1, P4, P5, P15, P18) were decapitated and the heads were fixed overnight in a freshly prepared solution of 4% paraformaldehyde. Some specimens were decalcified for $2{\sim}4$ weeks in a solution containing 10% of the disodium salt of ethylenediamine-tetraacetic acid (EDTA). Next, tissues were dehydrated, embedded in paraffin and sectioned serially at $6{\mu}m$ in thickness. Polyclonal antiserum raised against PDLs22 peptides, ISNKYLVKRQSRD, were made. The localization of PDLs22 in tissues was detected by polyclonal antibody against PDLs22 by means of immunohistochemical staining. The results were as follows; 1. Expression of PDLs22 protein was not detected in the tooth germ of bud and cap stage. 2. At the late bell stage and root formation stage, strong expression of PDLs22 protein was observed in developing tooth follicle, osteoblast-like cells, and subodontoblastic cells in the tooth pulp, but not in gingival fibroblasts, ameloblasts and odontoblasts of tooth germ 3. In erupted tooth, PDLs22 protein was intensely expressed in PDL and osteoblast-like cells of alveolar bone, but not in gingival fibroblasts, mature osteocytes and adjacent salivary glands. 4. In the developing alveolar bone and mid-palatal suture, expression of PDLs22 protein was seen in undifferentiated mesenchymal cells and osteoblast-like cells of developing mid-palatal suture, but not in mature osteocytes and chondrocytes. These results suggest that PDLs22 protein may play an important role in the differentiation of undifferentiated mesenchymal cells in the bone marrow and PDL cells, which can differentiate into multiple cell types including osteoblasts, cementoblasts, and PDL fibroblasts. However, more researches should be performed to gain a better understanding of the exact function of PDLs22 protein which related to the PDL cell differentiation.

새로운 국내 분리 토끼출혈병바이러스(RHDVa)를 감염시킨 토끼에서의 경시적인 병리학적 변화와 조직 내 바이러스 항원 분포

박중원,전지은,양동군,박은정,김한,이명헌,황의경,이중복,우계형,Park, Jung-Won,Chun, Ji-Eun,Yang, Dong-Kun,Bak, Eun-Jung,Kim, Han,Lee, Myeong-Heon,Hwang, Eui-Kyung,Lee, Chung-Bok,Woo, Gye-Hyeong 대한수의학회 2012 大韓獸醫學會誌 Vol.52 No.2

Rabbit hemorrhagic disease is a highly acute and fatal viral disease caused by rabbit hemorrhagic disease virus (RHDV). Since first outbreak in Korea 1987, RHDV has been continually affected in the country, but the pattern of outbreak seem to be changed. In this study, to understand the pathogenesis of the new RHDVa serotype, we therefore carried out to inoculate RHDVa to rabbits, and to examine the sequential histopathologic changes and viral distribution. Macroscopically, various sized dark red or white spots or appearance were observed in the liver, lung, kidney uterus and ureter. In euhanized rabbits, significant pathologic findings such as infiltration of heterophils and mononuclear cells were observed at 24 hours after inoculation (HAI), and these were sequentially extended periportal to centrilobular area. However, in dead rabbits, severe hepatic degeneration and/or necrosis with relatively weak inflammatory responses were observed. RHDV antigens began to detect in liver, spleen, and lung from 12 HAI by PCR. Immunohistochemically, RHDV positive cells were seen in only liver from 24 HAI, and the degree of immunogen reactivity was stronger in dead rabbits than in euthanized ones. In conclusion, RHDVa caused the subacute or chronic infection accompanying low mortality and moderate to severe inflammatory reaction in rabbits, suggesting the possibility that RHD could become endemic.

심내막염 환자의 동종이식편 치환술 -1례 보고-

박중원,박국양,김웅한,Park, Jung-Won,Park, Guk-Yang,Kim, Ung-Han 대한흉부심장혈관외과학회 1997 Journal of Chest Surgery (J Chest Surg) Vol.30 No.8

Prosthetic valve endocarditis(PVE), although uncommon, is associated with significant mortality if the infection spreads into the paravavular structures with later abscess formation. However, combined antibiotic and surgical treatment is often successful. Accurate diagnosis by on echocardiography, effective myocardial protection during operation and increased surgical experience have improved the short-term and long-term outcomes for patients with PVE. A 35-year-old male had a history of replacement of aortic and mitral valve, and tricuspid annuloplasty on August 1994, was admitted due to sudden onset of aphasia, leftward deviation of both eyeballs and spiking fever and diagnosed of having PVE by echocardiography. Reoperation was done after 6weeks of antibiotic treatment. On the operative field, we could notice circumferential vegetation along aortic valve annulus, paravalvular leakage and abscess pocket. The mitral valve amlulus was healthy. The patient underwent redo aortic valve replacement using cryopreserved aortic homograft after radical debridement of infected issue. During the follow up of 7 months period the homograft was well functioning without recurrence of symptoms. 인공판막심내막염은 드물지만 합병증으로 판막주위가 감염으로 파괴되고 농양이 형성되는 경우가 있어 높 은 사망율을 가져온다. 그러나 적절한 항생제의 사용, 심장초음파 검사에 기초를 둔 조기 진단,수술시 효과적인 심근보호. 그리고 수술경험의 축적으로 인공판막심내막염에 있어서 보다좋은 장단기 결과를 얻을 수 있다. 35세 남자 환자가 94년 8월에 대동맥판막, 승모판막대치술과 함께 삼첨판륜성형술을 받은후 특별한 증상 없이 지내다가 갑자기 시작된 실어증, 양안구의 좌측편위, 고열로 입원하여 심장초음파 검사를 시행한 결과 인공판막 심내막염 소견을 보여 6주간의 항생제 치료후 수술을 시행하였다. 수술소견상 대동맥 판륜을 따라 증식물 소견과 판막 주위의 누출, 농양주머니가 형성되어 있었고 승모판막은 비교적 건강해 보였다. 수술은 감염된 조직의 전체적인 제거와 냉동저장된 대동맥 동종이식편을 이용하여 재대치 수술을 시행했다. 환자는 수술후 63일째 퇴원했으며 퇴원후 7개월 동안 외래 추적 관찰결과 특별한 이상소견 없이 지내고 있다.

디자인·공예 융합 교육 프로그램 사례연구 -국민대학교 「팀팀Class」를 중심으로-

박중원,Park, Jung-won 한국디지털정책학회 2021 디지털융복합연구 Vol.19 No.8

융합이라는 시대의 흐름과 더불어 상상력과 창조성에 기반을 둔 디자인·공예의 본질적 속성의 효과적 결합을 위해서는 학문적, 방법적 측면에서 다양한 시도가 필요하다. 본 연구는 2020년 2학기에 실행된 국민대학교 팀팀Class의 디자인·공예 융합 교육 프로그램의 운영 사례를 분석하는 것이다. 이를 위해 기존 문헌연구를 통한 디자인·공예 융합 교육 관련 중요 항목을 5가지(대상, 방법, 운영, 공간, 소통)로 구분해 분석하였다. 이를 통해 디자인·공예 융합 교육 프로그램에 대한 경험과 문제점, 그리고 향후 디자인·공예 융합 교육 프로그램의 운영에 적용 가능한 해결방안 제시에 본 연구의 목적이 있다. 본 연구에서는 국민대학교 팀팀Class 디자인·공예 융합 교육 프로그램으로 사례연구 대상을 한정하였으나 향후 지속적인 연구를 통해 향후 다가올 코로나(COVID-19) 이후 시대의 새로운 환경에 적용 가능한 디자인·공예 융합 교육 프로그램을 위해 후속 연구가 진행되어야 할 것이다. The tendency of the current times require education to focus on convergence, and the same applies to the essence of ceramics and design base imagination and creativity. For effective integration, a wide range of experimentations is required both in terms of academic and methodic approaches. This study analyzes the [TeamTeam Class] curriculum, converging ceramics with design initiated in the second semester (autumn semester) of 2020. Through reference materials on ceramics and design convergence education, it was possible to classify the following 5 categories: Subject, Method, Management, space and communication. The aim of the study is to find resolutions to overcome existing issues and problems in search of more effective methods. Although this study is based on convergence education, [TeamTeam Class] at Kookmin University, I hope to extend it further to also consider education after COVID-19.

국내 분리 토끼출혈병 바이러스(RHDVa)를 감염시킨 토끼 간장에서의 경시적인 초미세구조 변화와 apoptosis

박중원,전지은,박은정,김한,이명헌,황의경,김재훈,이중복,우계형,Park, Jung-Won,Chun, Ji-Eun,Bak, Eun-Jung,Kim, Han,Lee, Myeong-Heon,Hwang, Eui-Kyung,Kim, Jae-Hoon,Lee, Chung-Bok,Woo, Gye-Hyeong 대한수의학회 2013 大韓獸醫學會誌 Vol.53 No.1

In this study, to understand the pathogenesis of new rabbit hemorrhagic disease virus (RHDVa) serotype, we carried out to administrate RHDVa to rabbits, and to examine sequential electron microscopic changes and relationship between pathogenesis and apoptosis. TUNEL-positive cells began to be observed from 24 hours after inoculation (HAI) and the number of positive cells was slightly increased with the course of time. Whereas marked increase of positive cells was seen in the liver from the rabbits died acutely. Typical viral particles with cup-like projections and a diameter of 30~40 nm were detected in homogenized liver samples and tissues at 36 and 48, and 48 HAI, respectively. Ultrastructurally, glycogen deposition was observed from the first stage of hepatocellular degeneration by RHDVa infection and then, swelling and disruption of cristae of mitochondria by viral particles, swelling of smooth endoplasmic reticulum, vacuoles and vesicles were detected. Condensation, margination and fragmentation of chromatin were observed in degenerative hepatocytes at 36 and 48 HAI, indicating apoptotic bodies. These data offer that hepatocytic apoptosis by RHDV infection could be closely related with mitochondrial impairment in the hepatocytes.

안와종야의 분류 및 치험

박중원(Park Jung Won),정화선(Wha Sun Chung) 대한안과학회 1997 대한안과학회지 Vol.38 No.8

대한간학회지 제7차 추계학술대회 논문집 : 담관세포암의 발생기전

박중원 ( Park Jung Won ) 대한간학회 2001 Clinical and Molecular Hepatology(대한간학회지) Vol.7 No.3(S)

일정한 경사각을 가지는 마이크로 홀 피어싱 공정에 관한 연구

박중원(Jung-Won Park),구태완(Tae-Wan Ku),강범수(Beom-Soo Kang) 대한기계학회 2010 대한기계학회 춘추학술대회 Vol.2010 No.11

Piercing process in sheet metal forming is widely used to manufacture a specified and precise nozzle used for ink-jet printer and fuel injection component, and so forth, in various industrial fields. Until present, the piercing process has been focused on the hole manufacturing in the thickness direction of thin sheet material as a perpendicular one, and many researches have been conducted. In this study, a specific angled piercing process has been introduced to obtain the angled piercing hole from a thin sheet material. In the fuel injection nozzle, 4-piercing holes as non-axisymmetric position are required on one component, and each micro hole has the slope angle of 19.5o. To develop the angled piercing process technology, firstly, finite element analysis combined with the ductile fracture criterion was carried out by using DEFORM. Formability of the micro hole was investigated with regard to the shear surface, and the dimensional comparison between the numerical simulation and the experimental result was carried out.

특집(Special Review) - 약물 알레르기 : 약물 알레르기의 사회적 부담과 관리

박중원 ( Jung Won Park ) 대한내과학회 2014 대한내과학회지 Vol.87 No.6

Drug allergy exhibits a wide range of clinical features that partly reflect the diversity of the underlying responsible mechanisms. These range from non-immunologic idiosyncratic reactions to Gell and Coombs type 1, 2, 3, and 4 reactions. Consequently, a drug allergy may be difficult to differentiate from an adverse drug reaction. The prevalence of drug allergy varies but is assumed to account for 30% of all adverse drug reactions. In the U.S., 3.1-6.2% of all ward patients are admitted because of adverse drug reactions, and 5-10% of all out-patients or ward patients have suffered an adverse drug reaction. Nonsteroidal anti-inflammatory drugs (NSAIDs), antibiotics, and radiocontrast media are the most common causes of drug allergy, but with the recent introduction of molecular anti-cancer agents, the number of drug allergy cases by these agents is soaring. Drug allergy is an important cause of mortality in admitted patients, and 1 out of every 10,000 admitted patients will die because of a drug allergy. Approximately 30% of adverse drug reactions can be prevented if previous reactions have been monitored and managed adequately. In 2006, a regional pharmacovigilance program was launched in Korea. In addition, the Korean Institute of Drug Safety and Risk Management plans to develop a nationwide drug utilization review program to monitor adverse drug reactions and to provide relevant information from the program to health professionals working in hospitals and clinics, with the aim of preventing drug allergies. Recent studies have shown a strong association between human leukocyte antigen genotypes and the severe cutaneous adverse reactions (SCARs) induced by certain drugs. Genotype prescreening may contribute to the prevention of SCARs induced by culprit drugs such as carbamazepine, allopurinol, and abacavir. (Korean J Med 2014,87:647-651)

제 26 회 대한 천식 및 알레르기학회 추계학술대회 : " 한국의 성인 기관지천식환자의 삶의 질 평가를 위한 설문 - KAQLQ " 의 개발 및 유용성 평가를 위한 다기관 연구

박중원(Jung Won Park),조유숙(You Sook Cho),이순영,남동호(Dong Ho Nahm),김윤근(Yoon Keun Kim),문희범(Hee Bom Moon) 대한천식 및 알레르기학회 ( 구 대한알레르기학회 ) 1999 추계학술대회 초록집 Vol.1999 No.-