Effect of Ischemic Preconditioning on the Oxygen Free Radical Production in the Post-ischemic Reperfused Heart

박종완,김영훈,엄창섭,배재문,박찬웅,김명석,Park, Jong-Wan,Kim, Young-Hoon,Uhm, Chang-Sub,Bae, Jae-Moon,Park, Chan-Woong,Kim, Myung-Suk The Korean Society of Pharmacology 1994 대한약리학잡지 Vol.30 No.3

허혈전처치(ischemic preconditioning)의 재관류심근손상 보호작용과 그 기전을 규명하기 위한 연구의 일환으로 허혈전처치가 심근세포의 산소라디칼 생성능력에 미치는 영향을 검토하였다. 흰쥐 적출심장의 Langendorff 관류표본에서 실험적인 허혈(30분)-재관류(20분)손상을 유도하였고, 허혈전처치는 재관류손상 유도전에 5분 허혈-5분 재관류를 3회 반복하여 시행하였다. 허혈심근 재관류손상의 지표로 심수축기능, 세포질효소 유출, 칼슘 유입 및 미세형태학적 변화를, 그리고 심근세포의 산소라디칼 생성기전으로 xanthine oxidase system의 변동을 허혈전처치와 비전처치 재관류 심장들에서 비교검토하였다. 연구 성적은 다음과 같다. 1. 허혈전처치는 허혈-재관류 심장의 관상혈류량, 심박수, 좌심실압 등 심기능의 저하를 현저히 회복시켰다(회복률; 91%) 2. 허혈-재관류 심장에서 lactate dehydrogenase 유출증가는 허혈전처치에 의해 현저히 저하되었다. 3. 허혈-재관류 심근세포의 전자현미경상 미세구조는 허혈전처치시 비교적 잘 보존되었으며, 특히 myofibril 구조의 보존이 매우 뚜렷하였다. 4. 허혈-재관류시 산화성 조직손상 척도의 하나인 malondialdehyde 생성이 허혈전처치에 의하여 현저히 감소되었다. 5. 허혈전처치 심장에서 xanthine oxidase(D,O 및 D/O형)활성은 변화되지 않았으나 그 기질인 hypoxanthine의 조직함량은 현저히 감소되었다. 이상의 결과들로 부터 허혈전처치는 세포수준에서 허혈심근의 재관류손상을 방지하며, 허혈전처치에 따른 산소라디칼 생성 감소가 재관류손상 방지에 일부 기여할 수 있으리라 사료된다.an을 2주간 처치한 경우에도 영향을 받지 않았다. 이상의 결과로 미루어 아마도 losartan의 내피세포에 대한 작용은 constitutive NO 생성을 증가시키나 inducible NO 생성에는 영향을 미치지 않을 것으로 여겨진다.cium ion이 상당히 중요한 역할을 하는데, 특히 소뇌에서의 NO생성의 감소는 이들 약물들의 치명적 부작용인 tardive dyskinesia와 매우 깊은 관련을 추측할 수 있다. 그러나, 더 많은 약물들의 검색으로 일관적인 기본 결과가 필요 되고 또 각개 약물의 특정적 기전이 연구되기 위하여 현재 실험중이다.(신칭(新稱), Cystostereum subabruptum), 털융단버섯(신칭(新稱), Tomentella pilosa), 노란소나무무늬버섯(신칭(新稱), Asterostroma laxum), 붉은소나무비늘버섯(신칭(新稱), Hymenochaete cruenta), 가루소나무비늘버섯(신칭(新稱), Hymenochaete fuliginosa), 소나무비늘버섯(신칭(新稱), Hymenochaete tabacina), 갈색시루삔버섯(신칭(新稱), Inonotus radiatus), 벚나무진흙버섯(신칭(新稱), Phellinus pomaceus), 회주름구멍버섯(신칭(新稱), Antrodia crassa), 층주름구멍버섯(신칭(新稱), Antrodia serialis), 흰그물구멍버섯(신칭(新稱), Ceriporia reticulata), 겹친손등버섯(신칭(新稱), Oligoporus balsameus), 점박이손등버섯(신칭(新稱), Oligoporus guttulatus), 무른흰살버섯(신칭(新稱), Oxyporus cuneatus), 각목버섯(신칭(新稱), The protective effect of 'ischemic preconditioning (PC)' on ischemia-reperfusion injury of heart has been reported in various animal species, but without known mechanisms in detail. In an attempt to investigate the cardioprotective mechanism of PC, we examined the effects of PC on the myocardial oxidative injuries and the oxygen free radical production in the ischemia-reperfusion model of isolated Langendorff preparations of rat hearts. PC was performed with three episodes of 5 min ischemia and 5 min reperfusion before the induction of prolonged ischemia (30 min)-reperfusion(20 min). PC prevented the depression of cardiac function (left ventricular pressure x heart rate) observed in the ischemic-reperfused heart, and reduced the release of lactate dehydrogenase during the reperfusion period. On electron microscopic pictures, myocardial ultrastructures were relatively well preserved in PC hearts as compared with non-PC ischemic-reperfused hearts. In PC hearts, lipid peroxidation of myocardial tissue as estimated from malondialdehyde production was markedly reduced. PC did not affect the activity of xanthine oxidase which is a major source of oxygen radicals in the ischemic rat hearts, but the myocardial content of hypoxanthine (a substrate for xanthine oxidase) was much lower in PC hearts. It is suggested from these results that PC brings about significant myocardial protection in ischemic-reperfused heart and this effect may be related to the suppression of oxygen free radical reactions.

SE 프로세스와 연계한 시험평가 단계의 QFD 적용방안 연구

박종완,이재우,Park, Jong Wan,Lee, Jae Woo 한국시스템엔지니어링학회 2018 시스템엔지니어링학술지 Vol.14 No.2

The research and development stage can improve the goal level of the project through systematic operation and management of systems engineering. The test evaluation tasks in the R&D process have a lot of connections with the system engineering in terms of requirement management, and the methodology for deriving the requirements when establishing the test evaluation items has been consistently required. In this paper, we propose the process of effectively establishing and using QFD (Quality Function Deployment), a tool to manage customer requirement, and drawing up HOQ (House of Quality) in the search and exploratory development stage of the self - propelled mortar project.

군용 EO/IR장비의 시험평가 기준 정립방안

박종완,Park, Jong Wan 한국군사과학기술학회 2016 한국군사과학기술학회지 Vol.19 No.5

Development-concerned parties, business managers and test evaluators have experienced conflicts among themselves due to the absence of standardized criteria for military electro-optical(EO) and infrared(IR) devices in test evaluation planning and evaluation phases. Therefore, establishment of objectified test and evaluation criteria for EO/IR devices is required. This paper applies South Korea's weather measurement average of minimum 15 km for visibility range, 3 bar from Johnson criteria for EO device and 4 bar from NATO's STANAG-4347 for IR device for target type, and 50 % probability for evaluation criteria, respectively. Based upon these criteria, this paper will establish suitable criteria that are improved for development weapon system in consideration of required capability of demanding forces and field environment.

리튬 이온 전지용 Si/Mo 다층박막 음극의 전기화학적 특성

박종완,Park, Jong-Wan,Ascencio Jorge A. 한국재료학회 2006 한국재료학회지 Vol.16 No.5

Si/Mo multilayer anode consisting of active/inactive material was prepared using rf/dc magnetron sputtering. Molybdenum acts as a buffer against the volume change of the Silicon. Multilayer deposited on RT (reversible treatment) copper foil current collector to enhance adhesion between Silicon and copper foil. Deposited Silicon was identified as an amorphous. Amorphous has a relatively open structure than crystal structure, thus prevents the lattice expansion and has many diffusion paths of Li ion. When deposited time of Silicon and Molybdenum is 30 second and 2 second respectably, electrode has more capacity and good cycle stability. A 3000 nm thick multilayer was maintained 99% of the initial capacity (1624 $mAhg^{-1}$) after 100 cycles. As the increase of the multilayer thickness (4500 nm, 6000 nm), Si/Mo mutilayer anodes show aggravation cycle stability.

미래전 수행을 위한 합동특수작전의 발전

박종완 ( Park Jong Wan ) 한국군사학회 2016 군사논단 Vol.88 No.-

This research aims to prepare for the future warfare by enhancing level of special operations` jointness in battlefield situation of Peninsula. As North Korea conducted its fourth Nuclear test in beginning of this year, the crisis was created. Launching of medium and long range missile and ballistic missile of submarine and the fifth Nuclear test by North Korea that has the most powerful in history made Peninsula deeply get into serious crisis. According to continuous Nuclear threats by North Korea, Ministry of National Defense in Korea has announced its aggressive Korea Massive Punishment & Retaliation activity called KMPR instead of using defense system such as Kill Chain and KAMD in the past. Core force of KMPR primarily consists of multiple missiles capable of precision strike at once, strike force and the most elite special operation forces. Removal operation of KJU regime is the direct deterrence effect that people so far aware against of nuclear development by North Korea. Special operation forces are the most optimal force by accomplishing strategical missions such as demolition of North Korean Leaders and their utility and value are highly estimated. Literally special operation forces has prepared these missions and the active role theory recently disclosed is very inspiring. However, special operation forces are impossible to complete missions unilaterally because special operation require complicated operation`s shaping and situations and need exquisite operation progress. Awareness of special operation forces` utility is scarce in peacetime but considering future warfare, operation`s shaping, environment, operational effectiveness, contribution, and war cases that occurred in Peninsula, it is absolutely valuable. This is why we have to keep up our level of jointness for current task. Presently, each military service maintains special operation forces which nationally and strategically retain quick reaction forces level. To be against of Nuclear tests and long range missiles threats by North Korea in advance, it is believed that going for joint special operation command is the best way to develop our services and nation for upcoming future. Therefore, it is expected to perceive joint special operations` utility in Peninsula and active role by policy decision-makers through this study.

허혈/재관류 심장의 산화손상에서 미토콘드리아의 역할

박종완(Jong-Wan Park),전양숙(Yang-Sook Chun),김명석(Myung-Suk Kim) 대한약리학회 1996 대한약리학잡지 Vol.32 No.2

Restoration of the blood flow after a period of ischemia is accompanied by generation of toxic oxygen radicals. This phenomenon may account for the occurrence of reperfusion-mediated tissue injury in ischemic hearts. In in vitro studies, although oxygen radicals can be generated from a variety of sources, including xanthine oxidase system, activated leucocytes, mitochondria and others, the most important source and mechanism of oxygen radical production in the post-ischemic reperfused hearts is unclear. In the present study, we tested the hypothesis that the respiratory chain of mitochondria might be an important source of oxygen radicals which are responsible for the development of the reperfusion injury of ischemic hearts. Langendorff-perfused, isolated rat hearts were subjected to 30 min of global ischemia at 37˚C, followed by reperfusion. Amytal, a reversible inhibitor of mitochondrial respiration, was employed to assess the mitochondrial contributions to the development of the reperfusion injury. Intact mitochonria were isolated from the control and the post-ischemic reperfused hearts. Mitochondrial oxygen radical generation was measured by chemiluminescence method and the oxidative tissue damage was estimated by measuring a lipid peroxidation product, malondialdehyde(MDA). To evaluate the extent of the reperfusion injury, post-ischemic functional recovery and lactate dehydrogenase(LDH) release were assessed and compared in Amytal-treated and -untreated hearts. Upon reperfusion of the ischemic hearts, MDA release into the coronary effluent was markedly increased. MDA content of mitochondria isolated from the post-ischemic reperfused hearts was increased to 152% of preischemic value, whereas minimal change was observed in extramitochondrial fraction. The generation of superoxide anion was increased about twice in mitochondria from the reperfused hearts than in those from the control hearts. Amytal inhibited the mitochondrial superoxide generation significantly and also suppressed MDA production in the reperfused hearts. Additionally, Amytal prevented the contractile dysfunction and the increased release of LDH observed in the reperfused hearts. In conclusion, these results indicate that the respiratory chain of mitochondria may be an important source of oxygen radical formation in post-ischemic reperfused hearts, and that oxygen radicals originating from the mitochondria may contribute to the development of myocardial reperfusion injury.

천골열공을 통하여 삽입된 Flexible Fiberoptic Epiduroscope을 이용한 신경근병증 환자의 치험

박종완(Jong Wan Park),이정순(Jung Soon Lee),이주철 대한통증학회 1997 The Korean Journal of Pain Vol.10 No.2

Caudal epiduroscopy has been introduced as an altemative technique for direct injection of epidural steroid and lysis of adhesion. Futher, it gives a better understanding of the role of epidural adhesion in recurrence of sciatica and low back disorder after surgery. We experienced a clinical application of flexible fiberoptic epiduroscope inserted through the sacral canal. A 37-year-old woman was suffering from right lumbar radiculopathy after an operation for a herniated disc. A series of volumetric caudal steroid injections and physical therapy had little effect on her symptoms. Patient was thought to be a good candidate for epiduroscopy. Flexible fiberoptic epiduroscopy was as follows: 60 ml of normal saline irrigation and epidurogram, 40 mg of triamcinolone in 10ml of normal saline was directed around right LS nerve root. The following morning, patient reported reduced pain in her leg.

국내 구매사업 시험평가에 미치는 영향요인 분석

박종완(Jong Wan Park) 한국신뢰성학회 2015 신뢰성응용연구 Vol.15 No.4

Purpose : The purpose of this study is to analyse affecting factors of Test and Evaluation and to improve the institution of Test and Evaluation in the Domestic purchase program. Methods : The domestic acquisition program is currently small in terms of size, but it is gradually increasing every year. Still, the domestic purchase program has insufficient regulations and procedures compared to the other procurement methods including research & development. Various problems are likely to occur between test and evaluation group, and defense industries regarding the regulations during Test & Evaluation. Therefore, the regulations and methods need to be amended to upgrade the current domestic purchase program into an effective and flawless one. In this paper, six variables have been chosen by the help of experts working in Ministry of National Defense (MND), Joint Chiefs of Staff (JCS), Army Headquarters and DAPA. Results : The six variables are effectiveness of proposal evaluation, fairness of test and evaluation, sufficiency of operational capabilities, seasonal period of test and evaluation, fulfillment in Integrated Logistics Support (ILS) of the industries, and guaranteeing appropriate environment for related enterprises" participation. Conclusion : This paper can help defense industries to enhance their development capabilities, and to perform tests more fairly and effectively considering the key factors identified in this research.

무기체계 시험평가의 신뢰성 향상방안

박종완(Jong Wan Park) 한국신뢰성학회 2015 신뢰성응용연구 Vol.15 No.2

Test and Evaluation (T&E) have been verifying the level of it’s technological skill and the needed operational status of the development weapons. If the overall spectrum of test and evaluation is fulfilled substantially in the production & deployment, the needed level of the weapon system will be enhanced and also the reliability status will become higher considerably. We can know currently these issues through the mass media and all kinds of the news regarding the defense industry and programs. And so this article have studied the method of enhancing reliability of the test and evaluation, the 6 variables were selected through the discussion of the professional group. The the test and evaluation group needs consistently the professional training systems. After DT&E, we have to the event to verify the technical level of the development systems. We have to take the high level of the kinds of the environmental test. Scientific methods like system engineering will be adapted in process of the test and evaluation. The number of suitable test prototype in the test and evaluation is analysed more systematically. And we need to establish the standardization of the test and evaluation. If 6 variables are well analysed and adapted in the working field, the reliability of the test and evaluation will be considerably, the defense industry will take the chance to develope the future-oriented.

SOFC용 $\textrm{La}_{0.68}\textrm{Ca}_{0.32}\textrm{Cr}_{0.97}\textrm{O}_{3}$연결재료의 소결거동

이유기,박종완,Lee, Yu-Gi,Park, Jong-Wan 한국재료학회 1997 한국재료학회지 Vol.7 No.4

SOFC용 L $a_{0.68}$Ca/ sub 0.32/C $r_{0.97}$ $O_{3}$분말은 수산염법에 의해 제조되었고, 이 합성된 분말을 이용한 슬러리의 최적제조조건과 그린 필름의 최적 소성조건을 얻었으며,이 제조 조건에서 소결 시간과 온도에 따라 제조된 연결재료의 결정구조, 미세구조 및 소결거동을 각각X-선 회절, 주사전자현미경 그리고 에너지 분산 분광계를 이용하여 조사하였다. L $a_{0.68}$C $a_{0.32}$C $r_{0.97}$ $O_{3}$의 저온소결은 $Ca_{m}$ (Cr $O_{4}$)$_{n}$에 의해 이루어졌음이 관찰되었고, 이때 $Ca_{m}$ (Cr $O_{4}$)$_{n}$은 불규칙하게 녹아서 L $a_{1-x}$C $a_{x}$Cr $O_{3-}$$\delta$/와 반응하고 이 현상이 질량 이동을 증가시켜 시편의 급격한 결정립 성장과 조밀화를 야기시켰다. 이러한 결정립 성장과 조밀화는 소결온도 140$0^{\circ}C$부터 일어났다.X>부터 일어났다.