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유근피(楡根皮) 약침의 $NF-{\kappa}B$ 활성 억제능이 생쥐의 Type II Collagen 유발 관절염에 미치는 영향
이아람,변혁,박인식,정찬영,강민주,김은정,이승덕,김갑성,Lee, A-Ram,Byun, Hyuk,Park, In-Shik,Jung, Chan-Yung,Kang, Min-Joo,Kim, Eun-Jung,Lee, Seung-Deok,Kim, Kap-Sung 대한침구의학회 2007 대한침구의학회지 Vol.29 No.2
Objectives : The purpose of this study is to investigate the effectiveness of Ulmus davidiana Planch herbal acupuncture(UA) to inhibit nuclear $factor(NF)-{\kappa}B$ activation on type II collagen-induced arthritis (CIA) in mice. Methods : Using an in vitro test, the synoviocytes picked out from the experimental CIA mice were subcultured. The synoviocyte cells were treated with phorbol-12-myristate-13-acetate(PMA) for 1 hour prior to the addition of indicated concentrations($0.4\;-\;1.0mg/m{\ell}$) of UA, and the cells were further incubated for 24 hours. The in vivo test, $NF-{\kappa}B$ p65, inducible nitric oxide synthase(iNOS), cyclooxygenase-2(COX-2), vascular cell adhesion molecule(VCAM)-1 production and apoptosis was observed by immunohistochemical staining. Results : The PMA-induced $I{\kappa}B$ kinase(IKK), iNOS and COX-2 mRNA expression were dose-dependently decreased in UA treated synoviocytes. Using the in vivo test, the number of eosinophils in mice treated with UA noticeably decreased in the the CIA group(P<0.05 using student t test). In mice treated with UA, there was less cartilage erosion. less bone destruction, mild synovial hyperplasia, mild fibrosis, and mild angiogenesis with less MIP-2 production. By immunohistochemical staining, suppression of $NF-{\kappa}B$ p65, iNOS production, inhibition of COX-2 production, inhibition of VCAM-1 production and inducing apoptosis were observed. Conclusions : These results suggest that UA might be applicable to the therapy of RA to suppress $NF-{\kappa}B$ activation.
유근피(楡根皮) 약침의 $NF-{\kappa}B$ 활성 억제능이 생쥐의 Type II Collagen 유발 관절염에 미치는 영향
이아람,변혁,박인식,정찬영,강민주,김은정,이승덕,김갑성,Lee, A-Ram,Byun, Hyuk,Park, In-Shik,Jung, Chan-Yung,Kang, Min-Joo,Kim, Eun-Jung,Lee, Seung-Deok,Kim, Kap-Sung 대한침구의학회 2007 대한침구의학회지 Vol.24 No.6
Objectives : The purpose of this study is to investigate the effectiveness of Ulmus davidiana Planch herbal acupuncture(UA) to inhibit nuclear $factor(NF)-{\kappa}B$ activation on type II collagen-induced arthritis (CIA) in mice. Methods : Using an in vitro test, the synoviocytes picked out from the experimental CIA mice were subcultured. The synoviocyte cells were treated with phorbol-12-myristate-13-acetate(PMA) for 1 hour prior to the addition of indicated concentrations($0.4\;-\;1.0mg/m{\ell}$) of UA, and the cells were further incubated for 24 hours. The in vivo test, $NF-{\kappa}B$ p65, inducible nitric oxide synthase(iNOS), cyclooxygenase-2(COX-2), vascular cell adhesion molecule(VCAM)-1 production and apoptosis was observed by immunohistochemical staining. Results : The PMA-induced $I{\kappa}B$ kinase(IKK), iNOS and COX-2 mRNA expression were dose-dependently decreased in UA treated synoviocytes. Using the in vivo test, the number of eosinophils in mice treated with UA noticeably decreased in the the CIA group(P<0.05 using student t test). In mice treated with UA, there was less cartilage erosion. less bone destruction, mild synovial hyperplasia, mild fibrosis, and mild angiogenesis with less MIP-2 production. By immunohistochemical staining, suppression of $NF-{\kappa}B$ p65, iNOS production, inhibition of COX-2 production, inhibition of VCAM-1 production and inducing apoptosis were observed. Conclusions : These results suggest that UA might be applicable to the therapy of RA to suppress $NF-{\kappa}B$ activation.
X-ray를 통한 예풍(藝風)과 하관(何關)에서의 안전한 자침 깊이에 대한 고찰
변혁,강민주,정찬영,박인식,조현석,김경호,이승덕,김갑성,Byun, Hyuk,Kang, Min-Joo,Jung, Chan-Yung,Park, In-Shik,Jo, Hyeon-Seog,Kim, Gyeong-Ho,Lee, Seung-Deok,Kim, Kap-Sung The Korean AcupunctureMoxibustion Medicine Society 2007 대한침구의학회지 Vol.24 No.6
목적 : X-ray를 통해 안면마비에 다용되는 예풍과 안면통에 다용되는 하관에서의 안전한 자침 깊이에 대하여 고찰하여 보고자 하였다. 방법 : 건강한 지원자들에게 원치 않을 경우 언제든지 시험을 중지할 권리가 있다는 것을 공지한 후, 시험에 동의한 남녀 각각 2명의 피험자들을 대상으로 대학병원 침구과 전문의가 예풍과 하관을 직자(直刺)($40mm{\times}0.35mm$ 일회용 침)하였다. 피험자의 이상 반응 유무를 살피며 안면신경 혹은 삼차신경이 지나가는 경로로 알려진 깊이까지 진침(進鎬)하였다. 유침(留鍼) 상태에서 Skull X-ray의 AP view와 Lateral view를 촬영하였다. 결과 : 피험자들은 침병이 피부에 도달 하는 동안(40mm 직자) 자침 혈위에서 중창감(重脹感)을 자각하였으며, 추후 어떠한 이상 반응도 보이지 않았다. 결론 : 안면마비와 안면통의 효율적인 치료를 위하여 안면신경과 삼차신경에 근접할 수 있는 혈위인 예풍과 하관에서의 40mm 직자는 신경염이나 뇌 손상과 같은 이상 반응을 유발하지 않았다.
홍화자약침(紅花子藥鍼)의 항염증능이 백서의 LPS로 유발된 류마티스성 관절염에 미치는 영향
임대정,조재용,정웅채,박인식,김갑성,김경호,Lim, Dae-Jung,Cho, Jae-Yong,Jeong, Woong-Chae,Park, In-Shik,Kim, Kap-Sung,Kim, Gyeong-Ho 대한침구의학회 2008 대한침구의학회지 Vol.25 No.3
Objectives : This study is to evaluate Effect of Hong Hua Za acupuncture anti-inflammatory on rheumatoid arthritis that caused by Lipopolysaccharide in mouse. Methods : Hong Hua Za(Carthami Tinctorii Fructus) acupuncture(HA) was prepared and tested for therapeutic potential of rheumatoid arthritis, by measuring the inhibition of inducible nitric oxide synthase (iNOS) expression, nitric oxide(NO) production in vitro experiment, by measuring Tumpr Necrosis Factor-$\alpha$(TNF-$\alpha$) & Nuclear Factor kappa B(NF-$\kappa$B) p65 activation, synovial hyperplasia, angiogenesis and fibrosis in synovial membrane of knee joint of mice. Results : HA showed therapeutic effects on Lipopolysaccharide(LPS)-induced rheumatoid arthritis. HA inhibited iNOS mRNA and NO in RAW264.7cell of LPS-induced rheumatoid arthritis in a dose dependent manner. HA also showed significant inhibition of TNF-$\alpha$ & NF-$\kappa$B p65, activation, synovial hyperplasia, angiogenesis and fibrosis in synovial membrane of knee joint of mice. Conclusions : These results suggest that HA has an therapeutic effects on LPS induced-rheumatoid arthritis by inhibiting TNF-$\alpha$ activation.
약용약침(藥茸藥鍼)이 LPS 유발(誘發) 관절염(關節炎)의 윤활관절막내(潤滑關節膜內) 과다염증반응(過多炎症反應) 조절(調節)에 미치는 영향(影響)
정용래,이승덕,변혁,박인식,정찬영,이창환,김갑성,Chung, Yong-Re,Lee, Seung-Deok,Byun, Hyuk,Park, In-Shik,Jung, Chan-Yung,Lee, Chang-Hwan,Kim, Kap-Sung 대한침구의학회 2007 대한침구의학회지 Vol.24 No.4
Objectives: To evaluate expression of MIF mRNA, MIF, $TNF-{\alpha}$, $IL-6R-{\alpha}$, STAT-3, $NF-{\kappa}B$ p65, COX-2 and iNOS, MMP-9mRNA after injecting deer antler herbal acupuncture solution in a LPS rat model. Methods: The experiment was divided in category of the control group, RA group, and NA group. RA was induced in the mice via injecting 300ug/kg LPS. The deer antler herbal acupuncture solution 50ug/kg was applied on $ST_{35}$(犢鼻) and EX-LE201(內膝眼) for 19days from $3^{rd}$ day of RA inducement. Results: 1. In the deer antler herbal acupuncture solution treated RAW 264.7cell, the mRNA expression of cytokines, RA related inflammation factors, such as the MIF, COX- 2, iNOS, and MMP-g reduced concentration dependently. 2. In the deer antler herbal acupuncture treated mice's synovial membrane, decrease in the cell replication of synovial joint cells, regeneration of blood vessel, fibrosis and fibroblastic cells expansion were observed. 3. Positive reaction of RA-related cytokines MIF, $TNF-{\alpha}$, $IL-6R-{\alpha}$, STAT3, COX-2, iNOS, $NF-\;{\kappa}B$ p65, MMP-9 was reduced. Conclusion : On the basis of the results, it was concluded that deer antler herbal acupuncture extract has significant protecting ability against acute progressive RA by inhibiting the production of MIF, as a top in cytokines related to inflammation.