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타액내 구강질환 원인 균의 세균배양법, SYBR green qPCR법, MRT-PCR법 간의 정량분석
박용덕 ( Yong-duk Park ),오혜영 ( Hye-young Oh ),박복리 ( Bok-ri Park ),조아라 ( Ara Cho ),김동기 ( Dong-kie Kim ),장종화 ( Jong-hwa Jang ) 한국치위생학회 2017 한국치위생학회지 Vol.17 No.2
Objectives: The purpose of this study was to compare SYBR Green qPCR, TaqMan, and bacterial selective medium cultures for accurate quantitative analysis of oral microorganisms. Methods: The SYBR Green method is widely used to analyze the total amount of oral microorganisms in oral saliva. However, in this study, MTR-PCR method based on TaqMan method was performed using newly developed primers and probes. In addition, it was designed to confirm the detection agreement of bacteria among bacteria detection method. Results: As a result of MRT-PCR and SYBR Green qPCR analysis, more than 40 times (0.9-362.9 times) bacterium was detected by MRT-PCR. In addition, more bacteria were detected in saliva in the order of MRT-PCR, SYBR Green qPCR, and bacterium culture, and the results of MRB-PCR and SYBR Green qPCR showed the highest agreement. The agreement between the three methods for detecting P. intermedia was similar between 71.4 and 88.6%, but the agreement between MRT-PCR and SYBR Green qPCR was 80% for S. mutans. Among them, the number of total bacteria, P. intermedia and S. mutans bacteria in saliva was higher than that of SYBR Green qPCR method, and bacterium culture method by MRT-PCR method. P. intermedia and S. mutans in saliva were detected by MRT-PCR and MRT-PCR in 88.6% of cases, followed by the SYBR Green qPCR method (80.0%). Conclusions: The SYBR Green qPCR method is the same molecular biology method, but it can not analyze the germs at the same time. Bacterial culturing takes a lot of time if there is no selective culture medium. Therefore, the MRT-PCR method using newly developed primers and probes is considered to be the best method.
Garlic extract 배합 치약의 치주질환 균주에 대한 항균 효과
장종화 ( Jong Hwa Jang ),박용덕 ( Young Duk Park ),류다영 ( Da Young Ryu ) 한국치위생학회 2012 한국치위생학회지 Vol.12 No.3
Objectives: This study mean to confirm the antibacterial activity of a garlic extract widely culturing in our region and was to determine the effect of dentifrice containing 0.1% extracts of garlic on dental plaque and gingivitis in a double blind and clinical studies in 50 healthy adults aged from 20 to 22 years who provided a consent for their participation, Methods: The antibacterial activity was evaluated using triple distilled water and the Kirby-Bauer disk diffusion method and the minimal inhibitory concentration (MIC) against various pathogens for periodontal disease, such as P. gingivalis 381(ATCC33277), was estimated. The experimental groups classified according to the concentration of garlic extract used: 10,000ppm(A), 5,000ppm(B), 2,500ppm(C), 1,000ppm(D). Oral examination of subjects was performed through clinical periods and on day of baseline, 6, 12, 19, 25 days plaque index and gingival index were scored by Turesky` modified index and Loe & Silness index. After 12, 19, 25 days use of their respective dentifrices, statistically decreases of plaque index, gingival index were shown in both the experimental and the control group, respectively. Results: There was significant antibacterial activity in the "2,500ppm(C)"group against P. gingivalis 381. Experimental group exhibited significantly the lower plaque levels and the higher levels of gingival health by the use of the dentifrices contained extract of garlic from 6 days compare with control group(p<0.05). The degree of decrease was more significant on gingivitis level of the experimental group than the control group(p<0.05), Conclusions: This findings indicated that the oral products containing a garlic extract is effective in preventing and treating periodontal diseases, and has potential value in inhibiting periopathogens.
일부 서울소재 초등학교 학부모들의 치면열구전색 건강보험 급여화에 관한 인식 조사
김진경 ( Jin Kyoung Kim ),최영숙 ( Young Suk Choi ),박용덕 ( Yong Duk Park ) 대한예방치과·구강보건학회 2009 大韓口腔保健學會誌 Vol.33 No.4
Objectives: This study was investigated the recognition of oral sealant and health insurance, and to suggest the method for ideal policy and efficiency of sealant in oral health insurance. Methods: We implemented a self-administrated questionnaire to 500 parents of elementary school at 20 schools in a metropolitan area from May to June, 2009. The collected 388 questionnaires on which we performed statistical analysis. The statistical analysis included aby frequency analysis a correlation analysis and logistic regression using the SPSS WIN 15.0 software program. Results: We found that 94.8% of objectives were not recognized by parents answering this questionnaire on oral health insurance service. Expected cost of sealant ranged from 5,000 won up to 10,000 won. The opinion for whether or not oral sealant was responsibility of public health service was split 50% for and against. The reasons for these founding are attributable to reasonable cost(72.0%), and the reason for disagreement was distance to treatment(54.8%). Priority order of any additional items in health insurance cognition was to treat scaling(30.0%). The high levels of education and total income, was high cognition(p<.01) for health service insurance. Persons with higher total income demonstrated higher agreement with(p<.01) the responsibility being sole responsibility of public health service. Conclusion: The recognition of health insurance for sealant, besides widely classified advertising, as being highly effective in the prevention of cavitis is needed urgently. An increased expansion and access in prevention guaranteed reasonable costs. Also, we needed to review previous studies on the enforcement of oral sealant use in public health insurance.