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      • KCI등재
      • KCI등재

        타액내 구강질환 원인 균의 세균배양법, SYBR green qPCR법, MRT-PCR법 간의 정량분석

        박용덕 ( Yong-duk Park ),오혜영 ( Hye-young Oh ),박복리 ( Bok-ri Park ),조아라 ( Ara Cho ),김동기 ( Dong-kie Kim ),장종화 ( Jong-hwa Jang ) 한국치위생학회 2017 한국치위생학회지 Vol.17 No.2

        Objectives: The purpose of this study was to compare SYBR Green qPCR, TaqMan, and bacterial selective medium cultures for accurate quantitative analysis of oral microorganisms. Methods: The SYBR Green method is widely used to analyze the total amount of oral microorganisms in oral saliva. However, in this study, MTR-PCR method based on TaqMan method was performed using newly developed primers and probes. In addition, it was designed to confirm the detection agreement of bacteria among bacteria detection method. Results: As a result of MRT-PCR and SYBR Green qPCR analysis, more than 40 times (0.9-362.9 times) bacterium was detected by MRT-PCR. In addition, more bacteria were detected in saliva in the order of MRT-PCR, SYBR Green qPCR, and bacterium culture, and the results of MRB-PCR and SYBR Green qPCR showed the highest agreement. The agreement between the three methods for detecting P. intermedia was similar between 71.4 and 88.6%, but the agreement between MRT-PCR and SYBR Green qPCR was 80% for S. mutans. Among them, the number of total bacteria, P. intermedia and S. mutans bacteria in saliva was higher than that of SYBR Green qPCR method, and bacterium culture method by MRT-PCR method. P. intermedia and S. mutans in saliva were detected by MRT-PCR and MRT-PCR in 88.6% of cases, followed by the SYBR Green qPCR method (80.0%). Conclusions: The SYBR Green qPCR method is the same molecular biology method, but it can not analyze the germs at the same time. Bacterial culturing takes a lot of time if there is no selective culture medium. Therefore, the MRT-PCR method using newly developed primers and probes is considered to be the best method.

      • KCI등재
      • 국부펀드의 효율적 자산배분 - 상품펀드를 중심으로

        박용덕 ( Park Yong Duk ) 한국STATA학회 2022 The Korean Journal of STATA Vol.7 No.1

        국부펀드는 그 재원을 기준으로 상품펀드(Commodity Funds)와 비상품펀드(Non-commodity Funds)로 구분할 수 있다. 이 중에서 상품펀드는 국가가 보유하고 있는 원유 등 자원의 매각대금 또는 이로부터 발생하는 현금흐름(이하 배경자산)을 재원으로 한다. 자산배분관련 선행연구에 따르면 상품펀드의 자산배분 시 동 배경자산으로 부터 발생하는 위험을 고려하여야 하나, 2009년 이후 상품펀드와 비상품펀드의 채권에 대한 자산배분은 큰 차이를 보이지 않는다. 이러한 현상은 자산배분과 관련하여 첫째, 상품펀드의 경우 투자만기가 비상품펀드에 비하여 장기이어서 나타나는 위험자산의 증가효과(또는 채권의 감소효과)가, 둘째, 상품펀드의 배경자산인 석유와 주식시장과의 상관관계가 2009년 이후 양(positive)으로 전환되어 나타나는 위험자산의 감소효과(또는 채권의 증가효과)가 상쇄되어 나타난 것으로 설명할 수 있다. 이러한 점은 향후 석유와 주식과의 상관관계 변화시 금융시장에 어떠한 영향을 미칠지를 예측하는데 참고할 필요가 있을 것이다. SWF can be distinguished from commodity funds and non-commodity funds in accordance with its sources. Among these, commodity funds are sourced from oil and, or oil related ones, which are called background assets. Even though the background assets shall be considered in an asset allocation process, we may not find any meaningful discrepancy between the one of commodity funds and the one of non-commodity funds. Based on my research, it can be explained as below. Firstly, the investment horizon of the commodity funds is longer than the one of non-commodity funds. Secondly, the correlation between oil and stock market has been positive since 2009. These points illustrate that there will a need to look into the correlation between oil and stock market in a periodic manner and review whether there will be any impact on the stock market considering AUM of SWFs.

      • KCI등재

        수도권지역에서 노인의치 국민건강보험 급여에 관한 치과기공사의 인식조사

        이선경,박용덕,황경숙,김영훈,Lee, Sun-Kyoung,Park, Yong-Duk,Hwang, Kyung-Sook,Kim, Yung-Hoon 대한치과기공학회 2010 대한치과기공학회지 Vol.32 No.1

        Purpose: The purpose of this study is dental technicians perception investigation about national health insurance of old persons denture in metropolitan area. Methods: A survey was performed on 103 subjects in metropolitan from January 14 to April 30 in 2009. The research tool was a structured questionnaire. The collected data was analyzed by frequency, descriptive and multiple linear regression. Results: Total dental technician who respond to questionnaire was 103(100.0%). Male respondent appeared by 66.0%. 30-year-old low respondent appeared by 60.2% and subjects lived the most in seoul(68.0%). When got married, was expose that realization about national health insurance of old person s denture was high(p<0.05). When made denture, was expose that realization about national health insurance of old person s denture was high(p<0.05). Also, When got married, was expose that necessity about national health insurance of old persons denture was high(p<0.001). Conclusion: When did national health insurance of old persons denture, there were a lot of problems. But, old people was need it. Therefore, I desire that this study is used to basic data of the national health insurance.

      • KCI등재

        논문의 주저자 결정에 미치는 영향

        성정민 ( Jeong Min Seong ),박용덕 ( Yong Duk Park ) 한국치위생과학회 2011 치위생과학회지 Vol.11 No.2

        본 연구는 2010년 9월부터 2010년 12월까지 치위생학교육자 171명을 대상으로 연구윤리의 내용 중 저지순서 및 처자범위 결정에 대한 인식과 경험을 조사하고 다음과 같은 결론을 얻었다. 1. 본인연구의 저자 결정자가 누구냐는 질문에 55.6%의 과반수 이상이 ``지도교수``라고 응답했으며 저자결정의 경험도에서 내 연구에 참여하지 않았음에도 다른 사람을 공저자로 등재시킨 경험이 있느냐는 질문에 68.4%가 ``그렇다``라고 가장 높게 응답했다. 2 저자의 범위 및 순서결정 인식의 4점 척도 조사결과, ``결과해석이나 중요한 정보를 기여한 사람이 제 l저자가 되는 것은 아니다`` 2.81土.485로 가장 높게 나타났으며, ``초벌 번역을 한 사람에게는 공동저자의 자격이 있다``에 대해서는 2.60土.53으로 가장 낮게 조사 되었다. 3. 연구사회학적 특성과 저자자격 인식의 연관성 중 통계적으로 가장 유의하게 나온 항목은 주저자로서의 논문편수였으며, 이 중 주저자로서의 9-14편의 논문을 갖고 있는 응답자에서 ``데이터를 모으고 기록하며 문헌을 살피는 일을 도운 학생은 저자자격이 있다``와 ``초벌 번역을 한 사람에게는 공통저자의 자격이 있다`` 에서 각각 2.89土.315, 2.79土.419로 가장 높게 조사돼 통계적으로 유의한 차이를 보였다(p<0.05). 4. 저자자격 인식간의 관련 변인간의 상관관계를 분석한 결과, 모두 정의 상관관계로 나타났으며, 이 중 ``데이터를 모으고 기록하며 문헌을 살펴는 일을 도운 학생도 저자자격이 있다``와 ``초벌번역을 한 사람에게는 공동저자의 자격이 있다``가 가장 높은 상관관계 (r=.433)로 조사돼 통계적으로 유의한 차이를 보였다 (p<0.0l). 결론적으로 연구자간의 갈등을 줄이기 위해서는 저자결정에 대한 기준을 설정하고 이를 교육하는 것이 시급할 것으로 사료된다. 또한 그 과정에서 당사자들의 논의가 중요하고 이러한 논의와 합의 과정을 통해 연구자간의 갈등이 생기지 않도록 하는 것이 최선의 방법이라고 판단된다. The purpose of this study was to examine the professors, who teach the dental hygiene program in Korea, their Awareness on authors determined among research ethics. Three hundred and six full time professors and four hundred and eighty four part time professors in seventy eight universities all around Korea were surveyed. The following are the results that the collected data was carried out statistical analysis by using SPSS 12.0 program. The results was as followed. 1. The present study`s author makers, 95 respondents (55.6%) experienced advising professor and 67 respondents (39.2%) experienced personally. 2. As recognition about range and order decision of the authors, they expressed the biggest recognition that person who did the interpretation of the results or gave important information doesn`t always become the first author of the article(2.81±.485). 3. Between general characteristics and authorship recognition level, they were statistically significantly different with number of articles published as the first author(p<0.05). 4. Regarding the correlation between students who helped with collection of data and references also have the right to be the author of the research article and Person who did the rough draft translation also has the right to be the co-author of the research article had a statistically significant correlation of .433 which was the highest correlation factor (p<0.01). Conclusions, rules and regulations on research ethic should be more publicized through educational institutions.

      • KCI등재
      • KCI등재

        세치제에 함유된 비효소계 항산화제의 경시변화에 따른 잔류량

        박정은 ( Jung-eun Park ),박용덕 ( Yong-duk Park ),홍태기 ( Tae-gi Hong ),장종화 ( Jong-hwa Jang ) 한국치위생학회(구 한국치위생교육학회) 2016 한국치위생학회지 Vol.16 No.5

        Objectives: The purpose of this study is to evaluate the non-enzymatic antioxidants stabilities in dentifrices by ascorbic acid and tocopherol according to the chemical condition. Methods: For the analysis of two antioxidants, HPLC UV detector system was used. HPLC was performed using sodium sulfate, acetonitrile(ACN), methanol(MeOH) and measuring absorbance at 240-295 nm. To confirm general pH reaction of two compounds, buffer solution was prepared for the analysis. The dentifrice was titrated by pH so as to examine the change of elapsed time in dentifrice. Linearity of calibration curve of two antioxidants was measured. Results: Each compound showed good linearity at optimized wavelength as well as showing good precision. General pH reaction of two antioxidants was examined. Ascorbic acid showed the highest residue(63.23%) at pH 10 and the lowest residue(2.77%) at pH 4. Tocopherol showed the highest residue(55.70%) at pH 7 and the lowest residue(3.31%) at pH 4. As a result of changing elapsed time of antioxidants in dentifrice by pH, components were remained stably at low temperature(39.2℉) and pH 7. Conclusions: It is necessary to keep dentifrice including ascorbic acid and tocopherol, and non-enzymatic antioxidants at pH 7 and low temperature for improving chemical stability.

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