아토피피부염에서 보습제 사용

박영립 ( Young Lip Park ) 한국피부장벽학회 2007 한국피부장벽학회지 Vol.9 No.1

Atopic dermatitis(AD), one of the most common chronic inflammatory skin disease, is characterized by dry and itchy skin. Environmental influence on the disease process is complex, and the role of the stratum corneum(SC) as a biosensor, regulating the metabolic response to a variety of exogenous insults, appears to be essential. The water content of the SC is decreased in patients with AD. Dysfunction in the permeability barrier of the stratum corneum, as measured by the transepidermal water loss (TEWL), is considered to be one of the most important etiologic factors in AD, as an impaired barrier function allows the penetration of irritants and allergens which trigger the development of dermatitis. These impaired functions of atopic skin are probably related to abnormalities in lipid components of the SC. Clinically, some moisturizers improve the skin barrier function of atopic dry skin. Moisturizers are the obvious treatment for dry skin, and if used properly are useful treatment adjuncts in inflammatory dermatoses including AD. Not only the active ingredients, but also the excipients in moisturizers, which are incorporated mainly to improve stability and viscosity, affect the structure and function of the skin. Moisturizers usually contain humectants to enhance the water-binding capacity of the SC. Moisturizers have multifunctional effects. Desired properties include reduction of clinical signs of dryness, like scaling and roughness, and decrease in perceived feelings of tightness and itching. Moisturizers are considered very safe, especially when compared to some drugs eg, topical corticosteroids. However, adverse skin reactions from topical moisturizer preparations are not uncommon. The most common adverse reactions are sensory or subjective sensations immediately after application of a topical product. Systemic side effects of moisturizers are extremely rare. Ingredients reported to be capable of inducing systemic toxicity are salicylic acid and propylene glycol. The SC should be protected against deleterious substances and strengthened by the use of moisturizers in patients with atopic dermatitis. Ingredients such as emulsifiers, pH, chelating agents, antioxidants, and preservatives may need to be addressed in future studies on patients with atopic dermatitis.

Epithelioid Hemangioma 2예 - Angiolymphoid Hyperplasia with Eosinophilia -

박영립(Young Lip Park),황규왕(Kyu Uang Whang),김영근(Young Keun Kim) 대한피부과학회 1992 대한피부과학회지 Vol.30 No.5

Epithelioid hemangiomo, is belong to a group of unusual vascular proliferation accompanying prominent eosinophilic infiltration. It is usually classificed as a subset of angiolymphoid hyperplasia with eosinophilia despite of its peculiar endothelial morphology(i.e., vacuolization and hobnail appearance etc.). Histopathologically, the lesion was characterized by exuberant. proliferation of capillaries, epithelioid-appearing erdothelial cell, endothelial cytoplasmic vacuolization, solid sheets of endothelial cells, massive eosinophilic infiltration and lymphoreticular hyperplasia. The lesions are most common in he head-neck region and are characterized by single or multiple smooth-top papules or plnqu s of varying colors. We report two cases of epithelioid hemangioma occurring on the left auricle and scalp. The chiet complaints were pruritus and they have been early bleeding even in trivial trauma. Microscopically, the lesions were consisted of a prolifration of small to medium-sized blood vessels surraunded by inflammatory infiltrates predominantly composed of lymphocytes, histiocytes and eosinophils. The vessels were lined by epithelioid-appearing endothelial cells haveing enlarged round nuclei and abundant easinophilic or clear cytoplasm. Some vessels showed luminal obliteration by proliferating epithelioid endothelial cells. Occasionally, blood vessels were lined by hobnailor scallop-shaped endothelial cells. In immunohistochemical finolings, the epithelioid endothelial cells or proliferated capillaries were immunoreactive to factor VII-related antigen and negative to lysozyme. (Kor J Dermatol 1992;30(5):727-736)

아토피피부염 학회보고서: 한국인 아토피피부염의 진단기준에 대한 연구

박영립 ( Young Lip Park ),김형동 ( Hyung Dong Kim ),김규한 ( Kyu Han Kim ),김명남 ( Myeung Nam Kim ),김진우 ( Jin Wou Kim ),노영석 ( Young Suck Ro ),박천욱 ( Chun Wook Park ),이광훈 ( Kwang Hoon Lee ),이애영 ( Ai Young Lee ),조상 대한피부과학회 2006 대한피부과학회지 Vol.44 No.6

Background: Atopic dermatitis (AD) is a chronic, relapsing inflammatory skin disease with genetic and environmental background. The diagnosis of AD depends on the clinical presentation and history because there are no objective laboratory tests. The criteria established by Hanifin and Rajka have become the standard for the clinical diagnosis of AD (Until now, we used conventional Hanifin and Rajka`s diagnostic guidelines). But diagnostic criteria for Korean have not been studied yet. Objective: The purpose of the present study was to establish the diagnostic criteria of Korean AD. Methods: We made out a draft for diagnostic criteria for Korean on the basis of the Hanifin and Rajka`s guidelines and published Korean journals. And we established the diagnostic criteria for Korean after collecting extensive opinions from dermatologic specialists in many university hospitals. Results: The major criteria of AD is similar to conventional diagnosic criteria, but three additional minor features(periauricular eczema, scalp scale, skin prick test reactivity) were significant for the diagnosis of AD in Korean patients. The other eleven minor features of the conventional minor diagnostic features were also significant. Conclusion: We established Korean diagnostic criteria for AD. Our result suggest that ethnic backgrounds influence the phenotype of AD and that additional three features need to be included in the Korean diagnostic criteria. Continued refinement of these guidelines will facilitate diagnosis in specific ethnic populations and in specific subgroups of patients. (Korean J Dermatol 2006;44(6):659~663)

장병성 선단 피부염 각질형성세포 아포토시스에 관한 연구

박영립 ( Young Lip Park ),황규왕 ( Kyu Uang Whang ),방성원 ( Sung Won Bang ),김영근 ( Young Keun Kim ),유희준 ( Hee Joon Yu ),손숙자 ( Sook Ja Son ) 대한피부과학회 1996 대한피부과학회지 Vol.34 No.5

Background: The cause of acrodermatitis enteropathica(AE) is closely related to zinc deficiency. Zinc is a potent inhibitor of endonuclease. Acute rises in the apoptosis in lymphoid and myeloid cell lines during zinc deficiency has recently been reported. The method of terminal transferase mediated dUTP biotin nick end labeling(TUNEL) is used in situ labelling of apoptotic nuclei in routine tissue sections. Bbjective : The purpose of this study is to clarify our hypothesis that apoptosis resulted from zinc deficiency might cause keratinocytes damages in AE. Method: We stained 6 AE biopsy specimen with TUNEL technique. Results : In acroderrratitis enteropathica, apoptotic keratinocytes were shown in the entire epidermis as compared to normal, controlled skin, in which it was found only at the uppermost layer of this stratified epithelium. Conclusion : This result suggests that apoptosis resulting from zinc deficiency might play a role in keratinocyte death in AE. (Kor J Dermatol 1998;34(5): 753-756)

표피세포 아포토시스에 관한 연구

박영립 ( Young Lip Park ),황규왕 ( Kyu Uang Whang ),박준홍 ( Jun Hong Park ) 대한피부과학회 1998 대한피부과학회지 Vol.36 No.1

Background: Apoptosis is a highly selective form of cell suicide with characteristic morphologieal and biochemical features, including chromatin condensation, formation of apoptotic bodies, and DNA fragmentation by the activation of endonucleases. Various cytokines and physical or chemical factors can provoke apoptotic changes in the skin. Objective : We investigated the cytotoxic effects with epidermal cytokines and their combinations, K+ ionophores, protein synthesis inhibitor(emetine), inhibitor of endogenous endonuclease(aurintricarboxylic acid, ATA), sodium azide, and retinoic acid witp human epithelial tumor cell lines(A431 cells) to examine the degree of induction of apoptosis in the epidermal keratinocytes. Methods : Induction of apoptosis was measured in cultured human keratinocytes, keratinocyte cell lines(A-431, HaCat, KB cells), cultured human melanocytes and malignant melanoma cell lines(SK-28, SK-30) using a mixture of ethidium bromide and acridine orange, DNA agarose gel electrophoresis and TUNEL staining. Results : l. In the A-431 cells, (1 to a certain degree, the combination of IFN-gamma and TNF-alpha could only induce apoptosis. Q2 most of K+ ionophores were observed to induce necrosis rather than apoptosis. Q3 emetine, a protein synthesis blocker, was found to induce apoptosis in a dose-dependent pattern. Q4 sodium azide at a concentration of 1% .induced apoptosis rather than necrosis. Q5 retinoic acid inhibit the beuvericin induced apoptosis. 2. In human keratinocytes, Ql more resistant in the induction of apoptosis than any cultured keratinocyte cell lines p aurintricarboxylic acid(ATA)-an endonuclease inhibitor, could inhibit UV induced apoptosis 3. In human keratinocytes and cultured keratinocyte cell lines, c-PAF inhibit the beauvericin induced apoptosis. 4. Human melanocytes is very resistant for the induction of apoptosis by beauvericin. 5. In the melanocytes and melanoma cell lines, sodium azide and beauvericin induced necrosis rather than apoptosis. Conclusions : The epidermis is continuously exposed to toxic factors which might induce cell death. With the above results, the induction of appeared to be rather resistant, epidermal cell apoptosis which may reflect the existence of some endogenous protective mechanisms in the epidermis to survive at certain toxic environments; melanocytes showed high expression of bcl-2 protein which could play a role in endogenous defense against toxic environments of the epidermis. (Korean J Dermatol 1998;36(1): 59-70)

경부에 발생한 골화 섬유점액종

박수지 ( Soo Jie Park ),김형동 ( Hyung Dong Kim ),이종석 ( Jong Suk Lee ),박영립 ( Young Lip Park ),황규왕 ( Kyu Uang Whang ) 대한피부과학회 2006 대한피부과학회지 Vol.44 No.11

An ossifying fibromyxoma is a benign neoplasm which usually presents in soft tissue. It is a rare tumor of uncertain differentiation which virtually always acts in a clinically-benign fashion. Most patients present with a small, painless, well-defined, often lobulated subcutaneous mass, which affects the extremities, or less commonly the trunk, head and neck, and mediastinum. Its typical microscopic appearance is that of lobulated nests of uniform, round-to-oval shaped cells, separated by fibromyxoid stroma, in which an incomplete marginal shell of mature bone can be seen. We herein report the first case of an ossifying fibromyxoid tumor of the neck in Korea. (Korean J Dermatol 2006;44(11):1370~1373)

레티노이드에 의한 악성 흑색종 세포주의 bcl - 2 발현에 관한 연구

방성원,박영립,황규왕 ( Sung Won Bang,Young Lip Park,Kyu Uang Whang ) 대한피부과학회 1997 大韓皮膚科學會誌 Vol.35 No.6

Background: Apoptoss plays a major role in cellular proliferation and differentiation in tumor cells. Bcl-2, proto-oncogene, is known to inhibit apoptotic cell death of tumor cells. The high expression of bcl-2 in human melanoma cells over transforrned keratinocytes has been reported. The Loton group indicated that the growth of human melanoma cells exposed to ret.inoids was inhibit ed and their cellular melanin content incrensed over that of the untreat,ed ce1Ls. The Veis group reported that bcl-2 defieient mice showed hypopigmented hair. Which suggests that bcl-2 may in volve melanogenesis. The Above mentioned findings may suggest that. bcl-2 and retinoids may play a role in melanoms biology. Objective : We under ook this study to elucidate a possible relationship between retinoids and bcl-2 expvessions in human melanoma cell lines. Methods : We analysed bcl-2 expressions from SK 28 cells(melanoma cell lines) after pretreat ment with retinoids using flow cytometry and imtnunoblotting. Results : 1. In the results of the preliminary studies, we found that cultured human keratinocytes, fibro blasts and melanocytes n the resting state showed expressions of bcl-2. The latter showed a four fold expression of bcl-2. 2. Expression of bcl-2 was detected in SK 28, a human melanoma cell line, in the resting state. 3. After incubation with isotretinoin or etretinate treatment at 37'C, for 48 hours, this treated group showed a more ir creased expression of bcl-2 than the control group. Conclusion : Our data may explain that the mechanism of ret.inoids indur,ing inhibition of mela noma cell growth may be partly due to upregulation of bcl-2 expression. The high base-line ex pression of bcl-2 in melanoma cells may tell us why these pigment cells can survive against oxi dative products generated during melanogenesis. (Korean J Dermatol 1997;35(6): 1088-1094)

악성흑색종세포에서의 향신경요소 수용체 발현 양상

이정열,박영립,황규왕 ( Jung Youl Lee,Young Lip Park,Kyu Uang Whang ) 대한피부과학회 1997 대한피부과학회지 Vol.35 No.6

Background: Nerve growth factor(NGF), brain derived neurotrophic factor(BDNF), neurotropin 3(NT-3) and neurotropir-4/5 are neurotrophic factors necessary for the development and maintenance of specific neurors. The tyrosine protein kinase(trk) receptors exhibit specificity for differ ent neurotrophins. NGF is the cognate ligand for the trk A receptor, BDNF binds to trk B receptor and NT-3 binds to irk A, trk B and trk C receptors, Since melanoma cells are devived from neural ectoderm, growth factors which affect. neuronal tissue may have a role in melanoma biology. Objective : The purpose of this study is to demonstrate the presence of trk receptors in rnelanoma cells and observe th effect of K-252a on these melanoma cells growth and differentiation. Methods : After K252a over a range of 0-200nM was added into their cell lines, we exam ined cell viability of SK 28 and SK 30 cells. We performed this to examine the expression of the trk by flow cytometry and immunoblotting. Results : 1. The incubation of . K 28 cells and SK 30 cells with K 252a resulted in a dose dependent inhibition of cell proliferation. 2. In the flowcytometry, SK 28 cells and SK 30 cells showed a high expression of trk A and trk B, not trk C. 3. Using immunoblottiiig, trk in SK 28 cells and SK 30 cells was not expressed. Cpnclusions : These results indicate that. the identification of tyrosine protein kinase reeeptors and their inhibitor which affect differentiation and growth of a melanoma may provide an additional therapeutic option for treatment of melanoma. (Korean J Dermatol 1997;35(6): 1151-1158)

헤나 문신에 의한 알레르기성 접촉 피부염 1예

박준 ( Joon Park ),김형동 ( Hyoung Dong Kim ),조문균 ( Moon Kyun Cho ),박영립 ( Young Lip Park ),이종석 ( Jong Suk Lee ),황규왕 ( Kyu Uang Whang ) 대한피부과학회 2007 대한피부과학회지 Vol.45 No.10

전두부에 국한된 다발성 피지낭종 1예

박준 ( Joon Park ),노병화 ( Byoung Hwa Rho ),조문균 ( Moon Kyun Cho ),박영립 ( Young Lip Park ),이성열 ( Sung Yul Lee ),황규왕 ( Kyu Uang Whang ) 대한피부과학회 2007 대한피부과학회지 Vol.45 No.10