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이정섭,천민석,김옥봉,박종군,김시욱,박열,양영기,홍승환,박상대,Lee, Jung-Sup,Chun, Min-Suck,Kim, Ok-Bong,Park, Jong-Kun,Kim, Si-Wouk,Park, Yeal,Yang, Young-Ki,Hong, Seung-Hwan,Park, Sang-Dai The Korean Society for Integrative Biology 1995 동물학회지 Vol.38 No.4
분열형 호모인 Schizosaccbarornyces pombe에서 RecA 유사 단백질은 hydroxyurea (HU)나 methotrexate (MTX)와 같은 nucleotide pool 형성에 관여하는 효소의 저해제에 의해 유도발현된다. 본 연구에서는 대장균의 RecA 단백질과 S. pombe의 ribonucleotide reductase가 구조적으로 유사한지를 RecA 항체를 이용한 면역침전법과 [5-$^3$H]CDP를 기질로하는 thin layerchromatography방법을 통하여 알아보고자 하였다. S. pombe의 rinonucleotide reductase활성은 100mM HU에 의해 대조군에 비해 26.3% 저해되었으며, RecA 항체를 이용한 면역침전에 의해서도 43.3% 저해되었다. 이와 같은 결과는 S. pombe의 rinonucleotide reductase가 대장균의 RecA 단백질과 구조적으로 유사함을 시사하는 것이다. We have previoosly demonstrated that the RecA-like protein of Schizosaccharomyces pombe (S. pombe) is immunologically related to Escherichia coil (E. coil) RecA protein and that the cellular level of the protein is significantly increased by inhibitors of nucleotide pool-forming enzymes such as hydroxyurea (HU) and methotrexate (MTX) (lee and Park, 1994; lee et al., 1994). In this study, we report that the ribonudeotide redudase activity of S. pombe is inhibited by E. coil RecA antibody, as determined by thin layer chromatography using [5-$^3$H]CDP as a substrate. The relative activity of ribonucleotide reductase was dramatically inhibited by 100 mM of flu (26.4% reduction) in in vitro assay, compared to that of non-treated control. The ribonucleotide reductase activity was also inhibited by immunoprecipitation with E. coil RecA antibody (43.3% reduction). These results indicate that the strudure of S. pombe ribonucleotide reductase is in part similar to that of E. coil RecA protein.
류민정(Ryu Min Jeong),박열(Park Yeal) 한국인체미용예술학회 2006 한국인체미용예술학회지 Vol.7 No.3
Types of hair have been determined by heredity, because of genetic factors, curly hair is much more dominant than straight hair.<BR> This paper will provide fundamental data about damage to curly and straight hair when it is permed and dyed. For the purpose of reducing damage to the hair surface, observational comparative research was conducted.<BR> Perming and dyeing were treatments of one time and three times in 10days intervals, and in each case treated for 30 minutes at 25˚C and 50˚C respectively.<BR> When perming and dyeing were applied under the same conditions, i.e., temperature, duration time, frequency, material, cuticle of curly hair was damaged more severly than straight hair
미생물,발효,생물공학 : 효모세포의 전기융합 및 융합세포의 RAPD-PCR을 이용한 유전적 분석
김승 ( Seung Kim ),김재성 ( Jae Sung Kim ),박인성 ( In Sung Park ),조문구 ( Moon Gu Cho ),박열 ( Yeal Park ),전홍성 ( Hong Sung Chun ),최봉석 ( Bong Suk Choi ),박세은 ( Se Eun Park ),최한석 ( Han Suk Choi ),김명곤 ( Myung Kon Ki 한국응용생명화학회 2006 Applied Biological Chemistry (Appl Biol Chem) Vol.49 No.3
朴悅,盧永福 조선대학교 기초과학연구소 1984 自然科學硏究 Vol.7 No.1
Surveys on atmospheric phenomena, entomological investigation, antibody titers of amplifying hosts of Japanese Encephalitis Virus (JEV) have been carried out in the endemic area of Chunnam from 1981 to 1983. The results were as follows; 1. It was found that the monthly average air temperatures in January, June, July and August for the past 3 year were 0.5℃, 22.1℃, 25℃, and 26℃ respectively. 2. The Culex tritaeniorhynchus was first found at the begining of June. The density of the Culex tritaeniorhynchus was increased fast and reached the peak at the latter half of July. The status of the density was continued until the latter part of September. Comparing the density of the past 3 years, we found that of 1982 the highest. 3. Hemagglutination inhibition (HI) antibody titers in the sera of the swines aged older than 6 months and younger than 12 months reached higher than 50% during the days August 16 to 24 for the past 3 years. 4. H I antibody titers in the ducks tested showed 64.5% at the middle of September. 5. The time of the first occurrence was getting relaitvely earlier than usual.