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박순희,이슬,진효상,Park, Soon-Hi,Lee, Seul,Jin, Hyo-Sang 한국식품영양학회 2012 韓國食品營養學會誌 Vol.25 No.4
This research was carried out in order to discover acid-forming bacteria during fermentation of Makgeolli, as Makgeolli loses its commercial value due to overproduced acidic materials. In Makgeolli kept at $25^{\circ}C$, a sudden increase of acidity as well as the disappearance of yeast cells occurred at day 6, whereas the total cell count and bacterial type remained unchanged; the result implies that a succession of bacterial types, including acid forming bacteria, occurred. Two acid-forming bacteria were isolated from acidified Makgeolli and were identified as Acetobacter pasteurianus and Lactobacillus casei. When fresh and heat-treated Makgeolli were inoculated with Acetobacter pasteurianus and/or Lactobacillus casei, the greatest amount of acid was formed in Makgeolli inoculated with Acetobacter pasteurianus and Lactobacillus casei and also in Makgeolli with Acetobacter pasteurianus alone. This result indicates that Acetobacter pasteurianus is the main acidifier; furthermore, it shows the synergy effect in acid formation with Lactobacillus casei.
생리활성능을 지닌 사람 인터루킨 - 1β - 재조합 단백질의 대장균의 생산
박순희,박한길,나도선,최인성,정태화 ( Soon Hee Park,Han Kil Park,Doe Sun Na,In Seong Choe,Tai Wha Chung ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.3
We produced human recombinant interleukin-1β in E. coli to study the mechanism (s) of its pleiotropic action(s), and to develop it as an anti-cancer or anti-inflammatory drug. We constructed expression vectors using lambda P_L promoter. Expression of hrIL-1β in E. coli was induced and the production of the protein was confirmed by Western blot assay. We also confirmed the presence of biological activity of recombinant IL-1β protein by demonstrating its growth promoting effect on mouse thymocytes.
단백질 수송계 성분이 리보스결합 단백질의 수송에 미치는 영향
박순희,박찬규 ( Soon Hee Park,Can Kyu Park ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.3
To understand the mechanism of protein export in general and to identify component(s) of export machinery utilized by ribose-binding protein, we examined the effects of three prlA suppressors on the export of an export-defective mutant rbsB103. We also studied the requirement(s) of SecY protein and leader peptidase on the export of wild-type ribose-binding protein. The export defect of rbsB103 mutation was not suppressed by three prlA alleles tested. However, SecY protein appears to affect for the export of wild-type ribose-binding protein. Wild-type precursor ribose-binding protein was not processed by purified leader peptidase (lep product) in vitro.
대장균 리보스 결합단백질에 있어서 단백질 분비능력에 따른 삼차구조 변화
박순희,Linda L . Randall ( Soon Hee Park,Linda,L . Randall ) 생화학분자생물학회 1989 BMB Reports Vol.22 No.2
Three alleles of rbsB gene including export defective mutant were cloned for overproduction of proteins. Five different species of precursor and mature ribose-binding proteins were purified to homogeneity. The purified proteins were subjected to amino acid sequencing. The results confirmed the amino acid changes deduced from the DNA sequencing (Park et al., 1988a). Attempts were made to establish a correlation between export competency and conformation of the proteins by studies in vitro. In this regard, ligand binding ability, circular dichroism, and the sensitivity to the degradation by a protease were compared. However, there was no drastic conformational difference among these proteins in steady states. This results, in other aspect, implied that the proteins purified from the overproduced proteins are in native conformation and allowed further analysis for studying the mechanism of protein export in vitro.
황금·황련 추출물의 항균활성이 막걸리 저장성에 미치는 영향
박순희,이슬,진효상,Park, Soon-Hi,Lee, Seul,Jin, Hyo-Sang 한국식품영양학회 2012 韓國食品營養學會誌 Vol.25 No.4
This research was carried out to find herbal preservatives for Makgeolli, as Makgeolli loses its commercial value due to overproduced acidic materials. When Makgeolli was kept at $25^{\circ}C$ to find the changes in acidity, total microbial cell number, yeast cell number, and bacterial species variety, a sudden increase of acidity as well as the disappearance of yeast cells occurred at day 6, and Makgeolli was changed to complete off-flavor. Acetobacter pasteurianus is the main acidifier in Makgeolli and shows a synergy effect in acid formation when cultured in combination with Lactobacillus casei. Among 12 herbs, the ethanol extract of Sutellaria baicalensis showed antimicrobial activity against A. pasteurianus, whereas the ethanol extract of Coptidis rhizoma showed antimicrobial activity against L. casei. Makgeolli added with Sutellaria baicalensis extracts demonstrated a lower acidity than that with Coptidis rhizoma extracts, which indicates that the inhibition of an acetic acid former is more important than that of a lactic acid former in Makgeolli preservation. Sutellaria baicalensis extracts prolonged the shelf life of Makgeolli by 1~2 weeks at a minimal inhibitory concentration ($0.63mg/m{\ell}$) during storage at $10^{\circ}C$.
박순희,Park, Soon-Hee,Linda, L. Randall 생화학분자생물학회 1989 한국생화학회지 Vol.22 No.2
수송 결함을 지난 리보스 결합 전구체 단백질, 복귀 전구체 단백질 및 숙성단백질을 부분 또는 순수 정제하고 이들이 기질에 결합하는 것을 조사하여 대량생산된 리보스 결합 단백질들이 native conformation을 유지하고 있음을 확인하였다. 따라서 이들 단백질을 수송 능력과 구조 사이의 관계를 알아보기 위한 in vitro 분석에 사용하였다. 이들 단백질에 리보스가 결합하는 정도의 비교, circular dichroism 의 측정 및 proteinase K에 대한 내성을 조사한 결과 steady state에서 이들 단백질 사이에 현저한 구조적 차이가 없음을 밝혔다. Three alleles of rbsB gene including export defective mutant were cloned for overproduction of proteins. Five different species of precursor and mature ribose-binding proteins were purified to homogeneity. The purified proteins were subjected to amino acid sequencing. The results confirmed the amino acid changes deduced from the DNA sequencing (Park et al., 1988a). Attempts were made to establish a correlation between export competency and conformation of the proteins by studies in vitro. In this regard, ligand binding ability, circular dichroism, and the sensitivity to the degradation by a protease were compared. However, there was no drastic conformational difference among these proteins in steady states. This results, in other aspect, implied that the proteins purified from the overproduced proteins are in native conformation and allowed further analysis for studying the mechanism of protein export in vitro.
박순희(Sun Hee Park),김선욱(Sun Wook Kim),이선화(Seon Hwa Lee),박도준(Do Joon Park),박경수(Kyong Soo Park),김성연(Seong Yeon Kim),조보연(Bo Youn Cho),이홍규(Hong Kyu Lee) 대한내과학회 2000 대한내과학회지 Vol.58 No.5
N/A Background : Although it is generally accepted that high serum glucocorticoid levels causes osteoporosis by suppressing the bone formation, conflicting results have reported on bone resorption. But, previous studies have been carried out in patients with glucocorticoid excess secondary to exogenous glucocorticoid treatment of disorders, which also affect bone turnover and mass by themselves. The purpose of this study were to assess the effect of glucocorticoid excess on bone mass and turnover not influenced by other diseases known to affect skeleton and by different gonadal status and sex and to study the reversibility of osteopenia after cure of Cushing's syndrome. Methods : We measured bone mineral density using dual energy X-ray absorptiometry(DEXA) in 28 patients with Cushing's syndrome before and after surgical cure. In addition, in the patients with Cushing's syndrome and 21 healthy premenopausal women matched for age, we measured biochemical bone markers. Results : 1) Marked osteopenia was present in most patients with active Cushing's syndrome. Lumbar spine BMD and Ward's triangle BMD were significantly lower than femoral neck BMD and femoral great trochanter BMD.(Z-score: lumbar spine -2.22±1.17, femoral neck -0.71±1.08, Ward's triangle -1.77±0.97, femoral great trochanter -0.64±0.71; mean±SD, p<0.05). In patients with active Cushing's syndrome, serum osteocalcin, a marker of osteoblastic function, was reduced(p<0.05), and bone resorption was increased, as indicated by increased urinary N-telopeptide(p<0.05). 2) Osteopenia was still in patients after surgical cure.(Z-score: lumbar spine -1.59±1.01, femoral neck -0.47±0.58, Ward's triangle -1.44±0.77, femoral great trochanter -0.42±0.61). In Cushing's syndrome patients after surgical cure, serum osteocalcin and urinary N-telopeptide were higher compared to controls but not stastistically significant(p>0.05). 3) When compared with pretreatment values, BMD after surgical cure of Cushing's syndrome was increased significantly(p<0.05). Serum osteocalcin was increased and urinary N-telopeptide was decreased significantly(p<0.05). Conclusion : These results show that compared to age matched control, premenopausal Cushing's syndrome patients have reduced bone formation, increased bone resorption, and reduced BMD, especially trabecular bone and that these abnormalities are improved after surgical cure.(Korean J Med 58:560-567, 2000)