서울근교 약수터에서 분리한 Pseudomonas속균의 혈청생화학적 특성 및 항균제 감수성

박석기,김무상,함희진,김은정,황광호,Park, Seog-Gee,Kim, Moo-Sang,Ham, Hee-Jin,Kim, Eun-Jung,Hwang, Kwan-Ho 한국식품위생안전성학회 1998 한국식품위생안전성학회지 Vol.13 No.3

서울시내 약수에서 Pseudomonas속균의 균종분포, 생화학적 특성, 혈청학적 특성 및 항생제 감수성을 조사하기 위하여 약수 57개소에 대하여 봄, 여름, 가을에 걸쳐 조사하였다. 총 57개 약수를 조사한 결과, 33개 (57.9%)의 약수에서 Pseudomonas속균이 검출되었다. 봄, 여름, 가을에 모두 검출된 곳은 1곳(1.8%) 이었으며, 2회에 걸쳐 검출된 곳은 9곳 (15.8%)이었으며, 1회만 검출된 곳은 13곳 (22.8%)이었다. 지역별로는 청계산이 50%의 검출율을 나타내었으며, 북한산, 35.7&, 대모산 33.3%, 도봉산 29.6%, 수락산 25.9%, 우면산 22.2%, 불암산 7.4% 순이었다. 분리된 균종은 Ps. putidark 22주(50%)로 가장 많았으며, Ps. aeruginosa, Ps. fluorescens 및 Ps. mendocins 각 6주(13.6%), Ps.aureofaciens 4주(9.1%)이었다. 분리된 Ps. aeruginosa6주의 혈청형은 모두 5종으로 A형 2주 , B, C,E 및 G형 각 2주씩이었다. 분리된 Ps. aeruginosa의 항생제에 대한 내성은 Amoxicillin 90.9%, Chloramphenicol 84.1%, Carbenicillin 81.8%, Nalidixic acid 68.2%, Neomycin 38.6%, Streptomycin 31.8%, Gentamicin 4.6%, Kanamycin 4.6% 그리고 Colistin 2.3%이었다. Ps.aeruginosa는 다른 슈도모나스균에 비해 carbenicillin에 대한 감수성이 있으나 kanamycin에 대한 내성이 높았다. Ps. aureofaciens는 다른 슈도모나스균에 비해 streptomycin에 대하여 내성이 없었다. 분리된 Ps.aeruginosa의 항생제에 대한 다제내성양상은 5제내성이 31.8%로 가장 높았으며, 내성양상에서는 NA-CB-CTE-AMC가 18.2%로 가장 높게 나타났다. In order to investigate the species, serobiological characteristics and antibiotic sensitivity of Pseudomonas spp, we isolated Pseudomonas spp from 57 spring waters around Seoul area for spring, summer and autumn and identified Pseudomonas spp by biochemical characteristics and serological method. And also we tested the antibiotic sensitivity test by discdiffusion method. Of 57 spring waters tested, Pseudomonas spp were isolated from 33 spring waters(57.9%). Isolation rate of Pseudomonas spp in spring season was 28.1%, summer 21.1% and autumn 28.1%. Only 1 spring water was detected Pseudomonas spp in all seasons and 9 (15.8%) were detected for 2 seasons and 13 (22.8%) were for only 1 season. Isolation rate of Pseudomonas spp at Mt. Cheonggye was 50% and followed by Mt. Bookhan 35.7%, Mt. Daemo 33.3%, Mt. Dobong 29.6%, Mt. Surak 25.9%, Mt. Woomyun 22.2% and Mt. Bulam 7.4%. Of 44 Pseudomonas spp, 22 strains (50%) were identified by Ps. putida, Ps. aeruginosa, Ps. fluorescens and Ps. mendocina were identified 6 strains (13.6%), respectively. 4 strains (9.1%) were identified by Ps. aureofaciens. Of 6 Ps. aeruginosa, serotype A was 2 strains, B, E, G, and K was 1 strain, respectively. Of 44 Pseudomonas spp, resistance rate to amoxicillin was 90.9% and followed by chloramphenicol 84.1%, tetracycline 84.1%, carbenicillin 81.8%, nalidixic acid 68.2%, neomycin 38.6%, streptomycin 31.8%, gentamicin 4.6%, kanamycin 4.6% and colistin 2.3%. Ps. aeruginosa was more sensitive to carbenicillin than other Pseudomonas spp isolated from spring waters in Seoul area but more resistant to kanamycin, and Ps. aureofaciens was no resistant to streptomycin. Among multiple drug resistance, resistance to 5 drugs was 31.8%, 4 drugs 15.9%, 7 drugs 13.6%, 1 drug and 2 drugs 4.6%, and 8 drugs 2.3%, respectively. The multiple resistance patterns detected highestly were NA-CB-C-TE-AMC (18.2%), NA-CB-N-C-TE-AMC (13.6%), CBC-TE-AMC (11.4%) and NA-CB-N-C-TE-AMC-S (9.1%).

동물원(動物園) 야생동물(野生動物)에서 분리(分離)한 Yersinia enterocolitica 의 생물형(生物型), 혈청형(血淸型) 및 항생제(抗生劑) 감수성(感受性)

박석기,윤은선,김은정,Park, Seog-gee,Youn, En-sun,Kim, En-jung 대한수의학회 1994 大韓獸醫學會誌 Vol.34 No.1

A study on the isolation of Yersiniae from the feces of wild animals(mammals 376, birds 19 and reptiles 13) in zoo and the biotype and serotype and susceptibility of 12 antibiotics was carried. Out of 408 animals, Yersiniae were isolated from 28 animals(6.9%). Of 28 isolates, 27 isolates(96.4%) were Y. enterocolitica and 1(3.6%) was Y. kristensenii. According to the species, 25(6.6%) of Y. enterocolitica and 1(0.3%) of Y. kristensenii were isolated from 376 mammals, 2(15.4%) of Y. enterocolitica from 13 reptiles but not isolated from 19 birds. According to the eating pattern, 8(5.2%) of Y. enterocolitica were isolated from 155 carnivora, 13(10%) of Y. enterocolitica from 123 herbivora, and 6(4.9%) of Y. enterocolitica and 1(0.8%) of Y. enterocolitica from 123 omnivora. Out of 27 isolates of Y. enterocolitica, all were biotype 1. And predominant serotype was 0:21(40.7%), and 0:5(37.0%), 0:6(11.1%), 0:1(3.7%), 0:9(3.7%) and untypable(3.7%). Yersiniae isolated from zoo animals were resistant to cephalothin(100%), ampicillin(96.4%), carbenicillin(96.4%) and tetracycline(14.3%) and streptomycin(3.6%) and susceptible to chloramphenicol(100%), colistin(100%), gentamicin(100%), kanamycin(100%), nalidixic acid(100%), polymyxin B(100%) and tobramycin(100%). The predominant multiple resistance pattern was Am-Cf-Cb(82.1%), and Am-Cf-Cb-Te(10.7%) and Am-Cf-Cb-Te-Sm(3.7%).

돼지에서 분리한 Yersinia enterocolitica의 생물형, 혈청형 및 항균제 감수성

박석기,최철순,전윤성,Park, Seog-gee,Choi, Chul-soon,Jeon, Yun-seong 대한수의학회 1992 大韓獸醫學會誌 Vol.32 No.1

A study on the isolation of Yersinia from the feces of healthy pigs and the biotype and serotype and susceptibility to 16 antimicrobials was carried. Out of 853 pigs, Yersiniae were isolated from 349 pigs(40.9%). Of 349 isolates, 289 isolates(82.8%) were Yersinia enterocolitica and 54(15.5%) were Y kristensenii, 3(0.9%) were Y pesudotuberculosis and the rest 3(0.9%) were Y prederiksenii. Out of 289 isolates of Y enterocolitica, the predominants biotype was 3B comprising of 165 isolates(57.1%) and followed by biotype 2, comprising of 49 isolates(17.0%), bioptype 3A, comprising of 41 isolates(14.2%) and biotype 4 comprising of 23 isolates(8.0%). And the predominant serotype was 0 : 3 comprising of 231 isolates(79.9%) and followed by serotype 0 : 9 comprising of 42 isolates(14.5%) and 0 : 21 comprising of 10 isolates(3.5%). Y. enterocolitica were resistant to cephalothin(99%), novobiocin(99%), erythromycine(83%), ampicillin(83%) and carbenicillin(81%) and susceptible to amikacin(100%), colistin(100%), gentamicin(100%), kanamycin(100%), polymyxin B(100%), tobramycin(100%), chloramphenicol(99%), nalidixic acid(99%), neomycin(99%), streptomycin(99%) and tetracycline(99%). Most strains of biotype 2/serotype 0 : 9 were susceptible to carbenicillin(100%) and ampicillin(61%) but the other biotype/serotypes were resistant to these antibiotic.

Yersinia enterocolitica의 시험관내 병원성 성상, plasmid 보유 및 외막 단백질(OMP) 생산간의 관계

박석기,최철순,전윤성,Park, Seog-gee,Choi, Chul-soon,Jeon, Yun-seong 대한수의학회 1992 大韓獸醫學會誌 Vol.32 No.2

Two hundred and eighty nine strains of Yersinia enterocolitica isolated from healthy pigs were tested for the presence of 40~50 Megadalton virulence-associated plasmids and plasmidmediated in vitro virulence-associated properties, i.e., congo red uptake, calcium dependency, autoagglutination, CRMOX reaction, crystal violet binding and pyrazinamidase reaction. The correlationships between in vitro virulence-associated properties and the presence of 220 Kdalton outer membrane protein(OMP) were examined in strains with or without virulence-associated plasmids. The correlationships between the presence of plasmids on the production of the OMP and the expression of in vitro virulence-associated properties were studied with $CRMOX^+$ strains and acridine orangecured $CRMOX^-$ mutants. The results were as follows : 1. Of the in vitro virulence-associated tests with 289 strains of Y enterocolitica, 275 strains (95.2%) were positive for pyrazinamidase test, and followed by in order of crystal violet binding test, 226 (79.2% ) ; CRMOX test, 190 (65.7%) ; autoagglutination test, 1.85(64.0%) : calcium dependency test, 86 (29.8%) and congo red uptake test, 47(16.3%). 2. The correlationship between autoagglutination and CRMOX test(r=0.90) was highly significant (p<0.01). 3. In 190 strains(65.7%) bearing the virulence-associated plasmids(MW 40~50 Mdalton), the correlation between the presence of plasmids and their in vitro virulence-associated properties were highest with CRMOX test(r=0.93) and followed by in orders of AAG test(0.81), CV test(0.46), PYZ test(0.37) and CD test(0.18), but no correlationship between the presence of plasmids and CR test(-0.11). 4. The $CRMOX^+$ strains produced the 220 Kdalton OMP when they were cultured at $37^{\circ}C$, but not at $26^{\circ}C$. The presence of 220 Kdalton OMP was correlated significantly with in vitro virulence properties and the presence of virulence-associated plasmid, respectively. 5. In the isogenic $CRMOX^-$ mutant strains, of which plasmid were cured by treatment with acridine orange not only in vitro virulence-associated properties(CR 100%, CD 100%, AAG 82.6%, CV 58.3%) disappeared but also 220 Kdalton OMP(100%) was not produced. These results indicate that the positive CRMOX reaction is plasmid-mediated and the CRMOX test is potential as an in vitro virulence tests with Y enterocolitica.

소에서 분리한 Yersinia enterocolitica의 생물형, 혈청형 및 항균약제 감수성

박석기(Seog-Gee Park),최성민(Seong-Min Choi),오영희(Young-Hee Oh),최철순(Chul-Soon Choi) 大韓微生物學會 1993 大韓微生物學會誌 Vol.28 No.6

CRMOX 양성 및 음성 Yersinia enterocolitica의 배양세포 침습성과 세포독성

박석기(Seog Gee Park),최철순(Chul Soon Choi),전윤성(Yun Seong Jeon) 大韓微生物學會 1992 大韓微生物學會誌 Vol.27 No.4

시판 식물성 식품의 저장온도 및 저장기간별 세균균속의 변화

박석기(Seog Gee Park),이용욱(Yong Wook Lee),나승식(Seung Sik Na) 대한보건협회 2000 대한보건연구 Vol.26 No.2

N/A N/A

시판 동물성 식품의 저장온도 및 저장기간별 세균속도의 변화

박석기(Seog Gee Park),이용욱(Yong Wook Lee),윤희정(Hee Jung Youn) 대한보건협회 2000 대한보건연구 Vol.26 No.1

N/A N/A

서울 近郊 牧場水의 衛生化學的 分布 狀態

Seog Gee Park(朴錫基),Seong Won Kim(金成元),Kang Moon Lee(李康文),Bong Taek Lim(林鳳澤) 한국예방수의학회 1992 예방수의학회지 Vol.16 No.3

In order to investigate the bacterial sanitation of farm water in the suburb of Seoul, we examined Standard Plate Count, coliform, E. coli, heat-tolerant bacteria, psychrophilic bacteria, Pseudomonas aeruginosa and Enterococcus of 86 farm waters in Kwangju-gun, Namyangju-gun Ⅰ and Ⅱ, Pajugun. The results were as follows : 1. Of the 86 farm waters, the average number of psychrophilic bacteria was 1400±350 CFU/ml, SPC 1000±300 CFU/ml, Pseudomonas aeruginosa 290±150 CFU/100ml, Enterococci 190±100 CFU/100ml, Escherichia coli 130±60 CFU/100ml, coliform 31±12 CFU/ml and heat-tolerant bacteria 13±5 CFU/ml, respectively. 2. Of the 86 farm waters, the sanitary bacteriological conditions of only 24 waters (27.9%) were sufficient to animal and human. 40.9% of waters in Namyangju I region were sufficient to animal and human, 33.3% in Paju-gun, 19.1% in Namyangju Ⅱ region, and 18.2% in Kwangju-gun. 3. Fourty five waters (52.3%) were detected the standard plate counts of over than 100 CFU/ml, 35 waters (40.7%) were detected coliform, 33 (38.4%) heat-tolerant bacteria, 16 (18.6%) Pseudomonas aeruginosa, and 15 (17.4%) were detected coliform and enterococci, respectively. 4. There were highly significant between SPC and psychrophilic bacteria (r=0.792), coliform and E. coli (0.728), E. coli and heat-tolerant bacteria (0.636), coliform and enterococci (0.462), heat tolerant bacteria and coliform (0.451), and E. coli and enterococci (0.369).

江原 및 忠南地域 原乳 중 春夏期 衛生細菌의 分布

Seog Gee Park(朴錫基),Kang Moon Lee(李康文),Seong Won Kim(金成元),Bong Taek Lim(林鳳澤),Sung Bae Park(朴聖培),Yong Wook Lee(李容旭) 한국예방수의학회 1995 예방수의학회지 Vol.19 No.1

In order to investigate the bacteriological quality of in raw milk, we examined standard plate count, coliform bacteria, fecal Escherichia coli, mesophilic aerobic spores, psychrophilic bacteria, Pseudomonas aeruginosa and enterococci in raw milks collected from 58 farms in Choongcheongdo and Kangwondo Province according to spring and summer. In standard plate count, the I class(less than 100,000/ml) milks are 48(82.8%) in spring and 31(53.5%) in summer, II class(100,000~250,000/ml) milks are 2(3.5%) in spring and 4(6.9%), III class(250,000~500,000/ml) are 2(3.5%) in summer and 7(12.1%), IV class(500,000~1,000,000/ml) are 3(5.2%), 6(10.3%) and V class(over than 1,000,000/ml) are 6(10.3%) and 11(19.0%), respectively.