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박상신,민태진 ( Sang Shin Park,Tae Jin Min ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.1
The activity changes of the mitochondrial ATP synthase and ATPase illuminated with near-UV lights (300-400 nm) have been observed and the effects of FAD and NADH on the light activation of the enzyme have been investigated in mitochondria of Lentinus edodes. The mitochondrial ATP synthase and ATPase were stimulated by the illuminations of 330 nm and 380 nm, but inhibited by 350 nm and 330 nm compared with dark control group, respectively. FAD, a flavin, induced the activation of the mitochondrial ATP synthase by the liiumantions of activation (470 nm) and inhibition (350 nm) wavelengths. It, however, inhibited the activity of mitochondrial ATPase by the illuminations of activations (680 nm) and inhibition (330 nm) wavelengths. The activity of the mitochondrial ATP synthase which had been treated with flavin inhibitor such as phenylacetic acid (PAA) or potassium iodide (KI), was inhibited by 470 nm illumination. But, when the mitochondrial ATPase was illuminated with 680 nm in the presence of PAA or KI, the activity of it did not change. The activities of the enzyme with NADH were not affected by the illuminations of activation and inhibition wavelengths. From these results, it is suggested that a endogeneous flavoprotein serves as the photorecptor for blue light activation of the mitochondrial ATP synthase in L. edodes.
표고버섯 중의 광감응성 Mitochondrial ATPase의 광수용체에 관한 연구 -Mitochondrial ATPase의 광에 의한 활성화에 미치는 FAD 및 NADH의 영향-
박상신,민태진,Park, Sang-Shin,Min, Tae-Jin Korean Society for Biochemistry and Molecular Biol 1991 한국생화학회지 Vol.24 No.1
근자외선 영역 (300 nm-400 nm)의 각 파장별 광조사에 따른 mitochondrial ATP synthase는 330 nm의 조사에서 어두운 상태의 대조구에 비하여 가장 활성화되었으며, 350 nm에서는 그 활성이 억제되었다. 또한, mitochondria ATPase는 380 nm의 조사에서 가장 활성화되었으며 330 nm에서는 활성이 억제됨으로써, mitochondrial ATP synthase와는 상반된 경향을 보였다. Flavin은 470 nm의 광조사에 따른 mitochondrial ATP synthase의 활성화를 유발하였으며 680 nm의 광조사에 의한 mitochondrial ATPase의 활성도를 억제함으로써, ATP synthase의 청색광에 대한 광수용체의 역할을 하는 것으로 추정되며, flavin inhibitor의 PAA 또는 KI의 처리 후 470 nm의 조사에 의한 ATP synthase의 활성도 감소현상은 이를 뒷받침해준다. Mitochondrial ATP synthase 및 ATPase는 NADH의 존재하에서 각 활성화파장의 조사에 대하여 활성도 변화를 나타내지 않음으로써, 두 효소의 광에 의한 활성화와 무관함을 보였다. The activity changes of the mitochondrial ATP synthase and ATPase illuminated with near-UV lights (300-400 nm) have been observed and the effects of FAD and NADH on the light activation of the enzyme have been investigated in mitochondria of Lentinus edodes. The mitochondrial ATP synthase and ATPase were stimulated by the illuminations of 330 nm and 380 nm, but inhibited by 350 nm and 330 nm compared with dark control group, respectively. FAD, a flavin, induced the activation of the mitochondrial ATP synthase by the liiumantions of activation (470 nm) and inhibition (350 nm) wavelengths. It, however, inhibited the activity of mitochondrial ATPase by the illuminations of activations (680 nm) and inhibition (330 nm) wavelengths. The activity of the mitochondrial ATP synthase which had been treated with flavin inhibitor such as phenylacetic acid (PAA) or potassium iodide (KI), was inhibited by 470 nm illumination. But, when the mitochondrial ATPase was illuminated with 680 nm in the presence of PAA or KI, the activity of it did not change. The activities of the enzyme with NADH were not affected by the illuminations of activation and inhibition wavelengths. From these results, it is suggested that a endogeneous flavoprotein serves as the photorecptor for blue light activation of the mitochondrial ATP synthase in L. edodes.
박상신,김규하,이진갑,Park, Sang-Shin,Kim, Gyu-Ha,Lee, Jin-Kab 한국트라이볼로지학회 2007 한국트라이볼로지학회지 (Tribol. Lubr.) Vol.23 No.3
This paper presents measurement processes of rotational accuracy and comparison of theoretical values in the main bearing of scroll compressor. The main bearing is a type of oil journal bearing, but it has an axial or helical groove. The generalized coordinate system method, which can handle this groove, is applied to calculate the pressure profile in the journal bearing. The orbits of journal shaft are calculated corresponding to the compressed gas forces and bearing reaction forces. Then, the orbits are measured using three-point method. The results are compared to that from analyses.
박상신,이승렬,Park, Sang-Shin,Lee, Seung-Ryoul 한국트라이볼로지학회 2007 한국트라이볼로지학회지 (Tribol. Lubr.) Vol.23 No.3
A scroll compressor is on the increase in the use for the cooling and ambition machinery because of the advantages about high efficiency, low vibration and low noise. The design of thrust bearing for scroll compressor has depended on the experience. The lubrication considering the squeeze flow was applied for high side shell and low side shell of scroll thrust bearing. This work was based on governing fluid lubrication equation at the general coordinate. It shows the behavior for an orbiting scroll with direct numerical analysis using FDM. This study obtained the theoretical design value by finding load capacity and tilting angle of an orbiting scroll for thrust bearing in a scroll compressor. Especially this work performed the analysis about the design parameter. The program was written using Visual C++ to enhance user to change the design parameter easily. In particular the result value and the pressure profile were displayed as windows in every step for user to understand without difficulty.
박상신(Sang-Shin Park),김규하(Gyu Ha Kim),이진갑(Jin-Kab Lee) 한국트라이볼로지학회 2004 한국트라이볼로지학회 학술대회 Vol.38 No.-
This paper presents measurement processes of rotational accuracy and comparison of theoretical values in the main bearing of scroll compressor. The main bearing is a type of oil journal bearing, but it has an axial or helical groove. The generalized coordinate system method, which can handle this groove, is applied to calculate the pressure profile in the journal bearing. The orbits of journal shaft are calculated corresponding to the compressed gas forces and bearing reaction forces. Then, the orbits are measured using three-point method. The results are compared to that from analyses.