돼지 유행성 설사병(porcine epidemic diarrhea)의 상재화에 대한 혈청학적 증명

박봉균,한경수,류광수,김준영,정현규,Park, Bong-kyun,Han, Kyung-soo,Lyoo, Kwang-soo,Kim, Jun-young,Jeong, Hyun-kyu 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.4

The persistence of porcine epidemic diarrhea virus(PEDV) infection was demonstrated in 7 swine farms employing continuous pig flow management even after seasonal outbreaks. Clinically, sporadic postweaning diarrhea was a major concern in those farms. Subsequently circulatory antibody detection using serum neutralizing test made useful for confirmation of PEDV persistent infections. The persistence of PEDV in the premise might have induced recurrence over the period of time.

Binary Ethylenimine으로 불활화(不活化)한 Newcastle Disease Virus의 항원성(抗原性)과 면역원성(免疫原性)에 관한 연구(硏究)

박봉균,전윤성,이영순,이영옥,Park, Bong-kyun,Jeon, Yun-seong,Lee, Young-soon,Rhee, Young-ok 대한수의학회 1985 大韓獸醫學會誌 Vol.25 No.2

Effects of binary ethylenimine (BEI) treatment on the inactivation of infectivity and hemagglutinin of Newcastle disease virus (NDV) were studied in comparison with those of formalin treatment. Immune responses of chickens vaccinated with BEI-inactivated NDV vaccines were also investigated. The results were summarized as followings; 1. Complete loss of infectivity of NDV (Bl) was observed at 3, 7, and 24 hours after the treatment at $37^{\circ}C$ with BEI concentrations of 0.01M, 0.005M and 0.001M, respectively. 2. The hemagglutinin activity of NDV (Bl) remained constant when treated with 0.01M BEI at $37^{\circ}C$. However, it gradually decreased when treated with 0.1% or 0.2% formalin at $37^{\circ}C$. 3. When 4-week-old chickens were vaccinated with NDV vaccines prepared from Bl or Miyadera strains of NDV, inactivated with 0.1M BEI and adsorbed to aluminium hydroxide gel, favorable immune responses were observed throughout the 8 weeks of observation period. 4. When these chickens were revaccinated at 8 weeks after the first vaccination, strong anamnestic responses were evoked and the immunity maintained for 4 weeks of the observation. Though slightly bettor immune responses were observed after primary vaccination in chickens vaccinated with Bl vaccine compared with those vaccinated with Miyadera vaccine, the differences were not significant. 5. On the electron microscopy, BEI (0.01M) gave least effect to the envelope as well as capsid of NDV.

제3자 보관 전자정보 압수수색에서 피압수자의 법적 지위에 관한 고찰

박봉균(Park, Bong-Kyun) 한국법이론실무학회 2023 법률실무연구 Vol.11 No.4

무죄추정의 원칙과 공판전 인신구속 - 영장실질심사를 중심으로 -

박봉균(Park, Bong-Kyun),김문귀(Kim, Moon-Kwi) 한국법학회 2019 법학연구 Vol.74 No.-

무죄추정의 원칙은 유죄의 판결이 확정되기 전까지 피고인을 죄 없는 자에 준하여 취급함으로써 법률적·사실적 불이익을 주지 않는 것을 말한다. 이 원칙은 1980년부터 한국의 헌법과 형사소송법에서 명시적으로 규정된 후 형사소송의 공판단계 뿐만 아니라 다른 영역으로 적용범위가 확대되었다. 현재의 통설과 판례는 입증책임의 분배, 불이익 처우 금지, 인신구속의 제한원리를 무죄추정의 원칙 내용으로 일반적으로 받아들여지고 있다. 특히, 불구속 수사의 원칙은 무죄추정의 원칙에서 도출된다는 판례를 반영하여 2008년 형사소송법에 규정되었다. 그러나, 수사단계에서 구속은 유죄의 확정 효력과 무관하고 형사 절차를 확보하기 위한 구속을 불이익처분이라 볼 수 없다. 따라서, 무죄추정의 원칙은 수사기관에게 훈시적인 의미가 있고, 인신구속의 제한은 적정절차의 원칙, 비례성의 원칙으로 규율할 수 있다. 무죄추정의 원칙에 관한 그동안 연구성과는 피의자의 인권보장에 중점을 두고 고찰하였다. 따라서, 본 연구를 통하여 수사단계에서 무죄추정의 원칙을 수범자의 관점에서 재조명하고, 실천적 가치를 탐색함으로써, 무죄추정의 원칙 이외의 절차적 대안을 제시하는 것을 목적으로 한다. 이를 위하여 구속영장이 명령장 또는 허가장의 성격, 영장실질심사에서 구인영장의 직권발부에 대한 권력분립의 문제점, 미체포 피의자에 대한 영장실질심사 기간의 구속기간 산입배제 방식에 대한 검찰 실무의 문제점을 각각 다루었다. 특히, 경찰의 법정 구속 기간이 단 하루가 지났다는 이유로 구속영장이 발부된 피의자를 석방하는 등 인권보장에 있어서 최일선 기관임을 스스로 자처하는 검찰이 토·일요일 및 공휴일은 근무시간이 아니라는 이유로 구속의 필요성이 없는 피의자의 송치를 거부하는 이율배반적인 태도에 대하여 비판하였다. 우리나라는 피의자의 구속기간에 대하여 엄격한 제한을 가하고 있으나 미국, 일본, 독일에서는 피의자의 구속기간에 대한 일반적인 제한은 없다. 이러한 국가들이 무죄추정의 원칙이나 인권보장이 우리나라보다 미흡하지 않다면 구속기간의 제한을 두지 않는 이유에 관하여 향후 연구를 통하여 고찰해야 할 필요가 있다. The principle of presumption of innocence is to not give legal or real disadvantage by treating the defendant in the manner of an innocent until the conviction of the conviction is finalized. This principle has been extended to other areas as well as the stage of trial of the post - criminal proceedings explicitly stipulated in the Constitution and Criminal Procedure Act of South Korea since 1980. The current law and precedents are generally accepted as the principle of innocence in the distribution of burden of proof, prohibition of disadvantage treatment, and restraint principle. In particular, the principle of non-detention investigation is defined in the 2008 Criminal Procedure Law reflecting the case that the principle of innocence is derived from the principle of innocence. However, in the investigation stage, redemption is irrelevant to the ascertaining effect of guilt and can not be regarded as disadvantageous detention for securing criminal proceedings. Therefore, the guilty presumption principle has a meaningful meaning to the investigative institution, and the restraint of human restraint can be governed by the principles of proper procedure and proportionality principle. The results of the study on the principle of innocence presumption have focused on the guarantee of the human rights of suspects. The purpose of this study is to suggest procedural alternatives other than the principle of innocence by reexamining the principle of innocence in the investigation stage from the perspective of the practitioner and searching for practical value. In order to do this, the issue of the arrest warrant has the problem of the nature of the command or the license, the problem of separation of powers for issuing the warrant in the real examination of the warrant, the problem of the prosecution practice about the exclusion period of the arresting period Respectively. In particular, the prosecution, which prosecutes itself as the premier organization for guaranteeing human rights, including release of the suspects who have been issued arrest warrants for a single day, And to reject the prosecution of the accused. In South Korea, there are strict restrictions on the arrest period of suspects, but in the US, Japan and Germany there is no general restriction on the period of arrest of suspects. If these countries are not guilty of presumption of innocence or guarantee of human rights is less than that of Korea, it is necessary to investigate the reason why they do not limit the period of restraint through future research.

Protective Effect of Polygonum cuspidatum Aqueous Extract in Hyperosmolarity-induced Human Corneal Epithelial Cells

Bong Kyun Park(박봉균),Soo Wang Hyun(현수왕),Kyu Hyung Jo(조규형),Tae Gu Lee(이태구),Jin Sook Kim(김진숙),Chan Sik Kim(김찬식) 한국약용작물학회 2018 한국약용작물학회 학술대회논문집 Vol.2018 No.2

고소의 남용에 대한 대응방안

박봉균(Park, Bong-Kyun),김문귀(Kim, Moon-Kwi) 한국법이론실무학회 2020 법률실무연구 Vol.8 No.2

우리나라에서 고소의 남용은 어제오늘의 일이 아니다. 이 때문에 최근 경찰청에서는 형사고소가 있더라도 선별입건하는 대응방안을 제시하였고, 학계에서는 고소의 남용과 대응방안에 관한 여러 체계적인 선행연구를 수행하였다. 그러나, 선행연구는 공통으로 고소의 접수를 통제하거나 접수 후 형사조정하는 방안에 관심을 기울였고, 고소의 기본권 관련성은 충분히 다루지 않았다. 본 연구에서는 경찰 수사관의 시각에서 고소의 반려가 기본권으로서 재판절차진술권과 어떻게 조화를 이룰 것인지 살펴보고, 고소의 남용으로 인한 문제점으로 피고소인의 인권침해, 고소인의 무임승차 및 국가기관의 채권추심기관 경향을 지적하였다. 또한, 형사정책의 방향으로서 민사분쟁을 해결하기 위한 민간산업의 필요성, 고소사건에 대한 대심구조의 조사방식, 고소의 반려에 대한 입법화를 제시하였다. An amendment in 2020 to Criminal Procedure Act has balanced criminal investigation power between prosecutors and senior judicial police officers. Thus, the reform of a criminal complaint system is one of the justice polices on the criminal investigation implemented by Korea National Police Agency. Korea criminal justice system has longtime suffered from stigma characterized by ‘Kingdom of Criminal Complaint’. Criminal justice officers faced a tough challenge by frequent criminal complaints on a civil case. Accordingly, an efficient countermeasure is discussed among criminal justice officers to reject an improper criminal complaint. However sympathetic towards criminal justice officers’ feeling of being overburdened and the violation of suspects’ legal rights due to the abuse of criminal complaints, there might be a question that the legal protection may insufficiently provided for the victims of crimes. There has been a constitutional right of criminal victims’ statement on the trial since 1987. Nevertheless, the rejection of criminal complaints can unintentionally drive the constitutionally historical rights in peril because of the prohibition on private prosecution, monopoly of state on public prosecution, and firm existence of offense subject to criminal complaints. From the view points of criminal investigator, this research addresses in the literature review, the abuse of criminal complaints, the concept of victims of crimes, the precedent case of Constitutional Court regarding the right of statement on the trial, the rejection of criminal complaints, the offense subject to complaint. Conclusionally, a suggestion should be offered to foster the civilian industry of alternative dispute resolution. Also, both the prohibition of the abuse and the rejection process against the inappropriate criminal complaints are friendly stipulated in the Criminal Procedure Act.

韓國에서 츄잔病의 發生 및 binary ethylenimine 不活化 츄잔바이러스 豫防藥의 소에서의 免疫原性 試驗

Bong Kyun Park(朴奉均),Jae Chin Rhee(李在鎭),Soo Hwan An(安壽煥),Ho Kyou Moon(文琥奎),Kong Sik Kim(金恭湜),Dong Soo Son(孫東秀),Kwang Won Lee(李光源) 한국예방수의학회 1993 예방수의학회지 Vol.17 No.3

Chuzan disease of which the main clinical abnormalities were impairment of mobility and nervous signs took place in a calf breeding farm in Cheju province during the period of March, 1993. The serological survey indicated that antibody to Chuzan virus was present at a high rate in Cheju province, while it was at a low rate in the Honam area of the mainland. The retrospective sero-epidemiological investigations strongly suggested that Chuzan virus comes to Korea from the Southeast Asian countries and the first outbreak of Korea is simultaneous with that of Japan. A preliminary study for development of an inactivated Chuzan virus vaccine was performed. The binary ethylenimine treated-tissue culture fluid with a gel adjuvant had good immunogenicity in cattle. To control this disease in Korea, an inactivated Chuzan virus vaccine is scheduled for practical use in the field in the near future.

국내의 돼지 인플루엔자 바이러스(H1N1, H3N2)의 혈청학적 조사

윤재순,박봉균,한정희,Yoon, Jai-soon,Park, Bong-kyun,Han, Jeong-hee 대한수의학회 2007 大韓獸醫學會誌 Vol.47 No.3

Swine influenza is an acute, infectious respiratory disease caused by type A influenza viruses in pigs. In the previous studies, serological surveys have indicated the presence of H3N2 swine influenza virus (SIV) since 1995 in Korea. And the percentage of the antibody-positive rate was 39.12% in the survey determining the prevalence of H1N1 SIV antibodies in 2002. The purpose of this study was therefore to investigate the sero-prevalence of SIV regard to the age of the pig and the season between June 2004 and May 2005. In this study, a total of 932 sera were used. These sera were randomly selected from blood samples, which were submitted to Department of Veterinary Pathology, Kangwon National University and Department of Veterinary Virology, Seoul National University from June 2004 to May 2005. These sera have been tested by ELISA test kit (IDEXX Lab, USA) for the SIV H3N2, H1N1 respectively. SAS version 9.1 was used for the statistical analysis based on the age of the pig and the season. The overall sero-prevalence of the antibody against H3N2 SIV was 20.82% (194/932). The overall sero-prevalence of the antibody against H1N1 SIV was 37.23% (347/932). The overall dual sero-prevalence of the antibody against H3N2 and H1N1 SIV was 10.62% (99/932). H3N2 has significant difference in statistically regarding the age of the pig and the season (p<0.0001). H1N1 has significant difference in statistically regarding the age of the pig (p<0.0001) but has not significant difference in statistically regarding the season (p=0.5882).

아까바네 바이러스의 분리 및 RT-PCR 진단법에 관한 연구

조재진,이정길,박봉균,장정호,정정원,조인수,안수환,Cho, Jae-jin,Lee, Chung-gil,Park, Bong-kyun,Chang, Chung-ho,Chung, Chung-won,Cho, In-soo,An, Soo-hwan 대한수의학회 2000 大韓獸醫學會誌 Vol.40 No.1

Akabane disease is transmitted through mosquitoes in cattle, sheep and goats. It shows congenital abnormalities including encephalomyetitis, hydranencephaly, neurogenic arthrogryposis, and deformed neonatal calves. Akabane viruses, 93FMX and K-9 strain, were isolated from fetal matrix of aborted cow and blood of healthy cow, respectively. S gene sequences of 93FMX and K-9 showed 100% homology with that of OBE-1 strain isolated in Japan. Based upon our sequencing data, we synthesized specific primers for PCR diagnosis. Using these primers, we were able to amplify the S gene of Akabane virus not only from the culture fluid of Vero cells but also from the brain tissue of suckling mouse inoculated with, Akabane virus. These PCR products were confirmed by Southern blot hybridization. Not only the sensitivity of PCR test was high enough to detect the viruses of $10^{1.0}TCID_{50}/ml$, but also the time for diagnosis was significantly shorter than that of the virus isolation by tissue culture method. This method was also effective for the detection of Akabane virus in the cerebrum of fetus. RT-PCR method may be used for a useful diagnostic test of the clinical cases of Akabane disease.

개 파보바이러스성(性) 장염(腸炎)의 국내발생(國內發生)

이영옥,최대영,박봉균,한홍율,황의경,유규연,Rhee, Young-Ok,Choi, Dae-Young,Park, Bong-Kyun,Han, Hong-Ryul,Hwang, Eui-Kyung,Yoo, Gyu-Yeon 대한수의학회 1982 大韓獸醫學會誌 Vol.22 No.2

The disease syndrome characterized by the acute vomiting and diarrhea with high mortality had been greatly epidemic in Korea since June, 1931 and it was followed serologically and electron microscopically for the clarification of the agent. The agent present in feces of dogs associated with this syndrome had characteristic feature in agglutinating pig red blood cells that was specifically inhibited by anti-CPV reference dog serum. This also showed the serological identity with the reference CPV antigen in immuno-diffusion. Electron micrograph of the material revealed parvovirus particles with size of 20nm and icosahedral structure. These results clearly indicated that CPV was the primary cause of canine epidemic prevailing in 1981 in Korea.