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김용환 ( Yong Hwan Kim ),박종태 ( Jong Tae Park ),김재익 ( Jae Ik Kim ),김현중 ( Hyun Joong Kim ),김태순 ( Tae Soon Kim ),장미선 ( Mi Sun Jang ),김정남 ( Jeong Nam Kim ),박덕웅 ( Duk Woong Park ),배성열 ( Seong Yeol Bae ),박자윤 ( 한국가축위생학회 2008 韓國家畜衛生學會誌 Vol.31 No.2
This study was carried out to investigate the prevalence of fascioliasis of slaughtered and farmed cattle in Gwangju area from February to November in 2007. A total of 1,000 cattle fecal samples were collected from slaughter houses (n=805) and farms (n=185). Twelve (1.2%) were found as positive cases with excretion of the egg of Fasciola spp in the fecal specimens, and 128 (12.8%) were positive in intestinal parasitism using the flotation and sedimentation procedures. The infection rate of fluke larvae from the slaughtered cattle at abattoirs in Gwangju was 0.75%(6 out of 805 heads). In histopathology, there were several liver lesions such as inflammation with infiltration of eosinophil, polymorphonuclear cells, mononuclear cells and multinucleated giant cells, proliferation of connective tissue, calcification and abscess formation.
고바라다 ( Ba Ra Da Koh ),김현중 ( Hyun Joong Kim ),박덕웅 ( Duk Woong Park ),박성도 ( Seong Do Park ),김재익 ( Jae Ik Kim ),박종태 ( Jong Tae Park ),김용환 ( Yong Hwan Kim ) 한국가축위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.3
Bovine tuberculosis is an important zoonosis worldwide. Mycobacterium bovis, the causative agent of this disease in cattle, is also a pathogen for humans and several economically important animals. The cases of tuberculosis are reported in two cow found at slaughter house located in Gwangju city. Histopathologically, in the lymph nodes, granulomas consisted of large areas of necrosis surrounded by variable thick bands of cellular infiltrate containing macrophages, Langhans-type multinucleated giant cells and lymphocytes. Lesions in the lung followed the same developmental pattern as did lesions in the lymph nodes with some exceptions. With the acid-fast staining, numerous mycobacteria were revealed in the lung and lymph nodes. M bovis was confirmed as a causative agent in these cattle using bacterial isolation and PCR and restriction fragment length polymorphism method based on a unique 12.7kb fragment insertion sequence from the Mycobacterium tuberculosis genome and the pncA polymorphism. The insertion element IS6110 and IS1081 were present M bovis isolated from lungs and lymph nodes of cattle using PCR assay. These cases are interesting and important in public health aspect that M bovis-infected cattle were found during a routine post-mortem inspection at slaughter house.