3T3-L1지방세포 및 제2형 당뇨모델(KK-A<SUP>y</SUP>)에서 잎새버섯(Grifola frondosa) 조다당체 추출물의 항당뇨 효과

박금주(Kum Ju Park),오영주(Young-Joo Oh),이상윤(Sang-Yun Lee),김현수(Hyun-Su Kim),하효철(Hyo-Cheol Ha) 한국식품과학회 2007 한국식품과학회지 Vol.39 No.3

기존의 연구에서 보고된 잎새버섯의 항당뇨 효과에 대한 메카니즘을 설명하고자 본 연구에서는 잎새버섯의 인슐린 민감성 향상 제재로서의 가능성을 확인하고자 하였다. 이를 위하여 잎새버섯 열수추출물의 분획물을 분리하여 인슐린 저항성을 가진 3T3-L1 지방세포에서의 포도당 흡수 정도를 관찰하였다. 실험결과 HW-Ⅰ이 3T3-L1 지방세포의 인슐린 민감성을 향상시켜 세포 내로의 포도당 섭취를 대조군 대비 4.2배 향상시켰다. 제2형 당뇨 모델인 5주령 KK/A<SUB>y</SUB> 마우스를 3주간 적응시킨 뒤 HW-Ⅰ을 8주간 지유 식이 하면서 식이 섭취량, 음수 섭취량 및 체중변화를 관찰하였고 혈액중 포도당 농도, 혈장 인슐린 농도, 당화혈색소를 측정하였으며 결과는 다음과 같다. HW-Ⅰ 식이군은 control군과 비교하여 식이 섭취량 및 최종 체중간에는 유의적인 차이가 없었으나 음수 섭취량에 있어서는 control군에 비해 거의 2배정도 적은 섭취량을 보여 유의적인 차이를 나타내었다(p<0.001). 혈중 포도당 농도는 HW-Ⅰ 식이군의 경우 control군과 비교하여 식이 1주째 335.4±51.8 ㎎/dL로 유의적으로 낮은 혈당치를 나타내기 시작하여(p<0.01) 식이 8주째 311±96.8㎎/dL로 유의적인 혈당 억제 효과를 나타내었다(p<0.001). 혈장 인슐린 농도는 HW-Ⅰ 식이군의 경우 84.7±18.9 ng/㎖로 control군의 342.7±125 ng/㎖ 비교하여 24.7% 수준으로 유의적인 감소 효과를 나타내였다(p<0.001). 당화혈색소 농도는 HW-Ⅰ 식이군의 경우 8.99%로 control군의 9.87%보다 유의적인 감소 효과를 나타내었다(p<0.05). 이상에서 잎새버섯 열수추출물의 조다당체인 HW-Ⅰ이 인슐린 민감성 효과가 있는 것으로 밝혀졌으며 제2형 당뇨모텔인 KK-A<SUB>y</SUB> 마우스를 이용한 동물실험에서도 뚜렷한 혈당강하효과를 나타내어 인슐린 민감성 제재로 개발 할 수 있는 가능성을 보여 주었다. This study was performed to investigate the anti-diabetic mechanism of crude polysaccharides isolated from the fruiting bodies of Grifola frondosa. We treated 3T3-L1 adipocyte cells to observe whether the crude polysaccharides isolated from Grifola frondosa would stimulate insulin sensitivity. Significant insulin sensitizing activity was observed in the 3T3-L1 adipocytes, and giving the crude polysaccharide of Grifola frondosa with 1 nM of insulin caused glucose uptake to increase to a similar level as giving 50 nM of insulin alone. To confirm the mechanism for the anti-diabetic effect of the crude polysaccharides, we performed further examinations within KK-A<SUP>y</SUP> mice, an animal model of type 2 diabetes. The crude polysaccharides reduced blood glucose levels in the KK-A<SUP>y</SUP> mice for 2 weeks after feeding, and also significantly lowered plasma insulin levels. These results suggest that the anti-diabetic mechanism of the crude polysaccharide of Grifola frondosa is related to the enhancement of insulin sensitivity.

효소 , 대사산물 실크 피브로인 유래 펩타이드에 의한 RAW 264.7 Macrophage 의 Nitric Oxide 생성 촉진

박금주(Kum Ju Park),현창기(Chang Kee Hyun) 한국미생물생명공학회 2002 한국미생물·생명공학회지 Vol.30 No.1

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갱년기 여성 및 심혈관 건강을 위한 새로운 기능소재의 개발

최은혜(Eun Hye Choi),박금주(Kum Ju Park),강정일(Jung Il Kang),이상윤(Sang Yun Lee) 한국식품과학회 2020 식품과학과 산업 Vol.53 No.4

As Korea became the aged society, people pay more attention to their health and disease prevention. For a better and healthy aging, it is even more important for active seniors to manage their health. The development of functional ingredients targeted for active seniors, which may improve cardiovascular and menopausal women’s health, and their certification as individually recognized functional materials could serve as a new growth engine for the functional foods industry as well as contribute to the improvement of public health.

Comet assay를 이용한 방사선 조사육의 판별

정석규,박종흠,지승택,박금주,김해홍,현창기,신현길,Jeong, Seok-Kyu,Park, Jong-Heum,Ji, Seung-Taek,Park, Kum-Ju,Kim, Hai-Hong,Hyun, Chang-Kee,Shin, Heuyn-Kil 한국식품과학회 2000 한국식품과학회지 Vol.32 No.4

Comet assay를 이용하여 방사선 조사육의 조사여부 및 조사량을 판별해내는 방법을 개발하기 위해 1-10 kGy의 감마선 조사량으로 조사한 육조직에서 일어나는 DNA 손상을 측정하였다. Comet assay에 있어 최적의 comet image를 얻기 위해 세포 분리, 세포 lysis 및 전기영동에 대한 여러 조건들을 적용하여 최적의 조건을 확립하였다. DNA 손상도는 관찰되는 comet의 평균 tail length와 tail length에 의해 구분된 4 손상 등급에의 분포, 그리고 그 분포비율에 의해 본 연구에서 제시한 식에 의해 계산한 relative damage index(RDI) 값 등으로 비교 판정하였다. 평균 tail length와 RDI 값은 조사량이 증가함에 따라 증가하여 DNA 손상도가 증가됨을 나타내었으며, 평균 tail length으로는 조사량 간의 차이를 명확히 분별하기가 어려웠던 반면 RDI 값에 의하면 조사여부 및 조사량을 판별하기에 적합함을 알 수 있었다. 국내산 한우육과 수입 냉장육에 대하여 blind test를 실시한 결과 수입육이 높은 DNA 손상도를 나타내었는데 그 RDI 값은 방사선 조사에 의한 값보다는 비교적 낮은 것이어서 수입육의 DNA 손상은 방사선 조사가 아닌 저온처리의 결과인 것으로 판단되었다. 본 연구의 결과로부터 Comet assay가 우육의 방사선 조사여부 및 조사량 판별에 유용한 기술로 응용될 수 있을 것으로 판단되었다. DNA damages in post-mortem bovine muscle samples caused by gamma irradiation at doses of 1 to 10 kGy were determined by Comet assay. When the cell extract was prepared in a physical method and followed by neutral lysis and neutral electrophoresis, the optimal comet images could be obtained. DNA damages were evaluated from the mean tail length, the distributions of comet images in 4 groups divided by tail length and the relative damage index (RDI) values calculated from the distribution pattern. The mean tail length and RDI value were increased by increasing the irradiation dose, and the RDI value was found to be useful as an index for discriminating of irradiation and measuring the irradiated dose. Blind tests using Korean domestic (Hanwoo) and imported beef samples showed a higher RDI value for the latter. However, the value was lower than those of irradiated samples indicating that the cause of DNA damages in the imported beef samples might be not irradiation but low-temperature treatments. It was concluded from the results of this study that the irradiated beef and its irradiated dose could be detected and predicted by Comet assay.

Compound K의 인슐린분비 및 탄수화물 대사에 미치는 영향

최윤숙(Yun Suk Choi),한기철(Gi Cheol Han),한은정(Eun Jung Han),박금주(Kum Ju Park),성종환(Jong Hwan Sung),정성현(Sung Hyun Chung) 고려인삼학회 2007 Journal of Ginseng Research Vol.31 No.2

진세노사이드의 인슐린 분비 활성을 비교해 본 결과 PPD계열 진세노사이드가 인슐린의 분비를 촉진하는 경향을 보였으며, 그 중에서도 CK의 인슐린 분비 촉진 효과가 가장 뛰어났다. CK는 RIN-m5F cell line과 일차 배양한 췌장 소도세포에서 용량 의존적으로 인슐린의 분비를 촉진하였고 이러한 CK의 인슐린 분비 촉진 기전은 ATP-sensitive K? 채널의 봉쇄에 의한 것임을 확인하였다. H4IIE cell line에서 간세포내 당신생과 관련된 효소의 발현을 측정한 결과 CK는 dexamethasone/cAMP에 의한 PEPCK와 G6Pase의 발현을 억제하였다. 이로 미루어 볼 때, CK는 간에서 당의 신생을 억제하여 공복 시 혈당을 감소시킬 수 있음을 시사하였다. 또한 3T3-L1 cell line에서 TG의 함량과 PPAR-γ 유전자의 발현에 미치는 영향을 살펴본 결과 CK는 PPAR-γ의 발현을 억제하여 결과 지방세포의 분화를 억제하였다. 결론적으로 CK는 췌장에서 ATP-sensitive K? channel을 봉쇄함으로 인슐린 분비를 촉진시키고 또한 간세포에서 당 신생을 억제함으로 식후 및 공복 시 혈당을 감소시킬 것으로 기대된다. Compound K (CK) is a final metabolite of panaxadiol ginsenosides. Although panax ginseng is known to have anti-diabetic activity, the active ingredient is not yet fully identified. Therefore, it would be interesting to know whether and how CK has an anti-diabetic activity. First, insulin secretion-stimulating activity of CK was examined using RIN-m5F cell line and primary cultured islets. CK enhanced the insulin secretion in a concentration dependent manner. This effect, however, was almost completely abolished in the presence of diazoxide, K? channel opener, indicating that the insulin secretion-stimulating activity of CK is presumably due to blockade of ATP sensitive K? channel. In addition, effects of CK on gene expressions of hepatic enzymes (phosphoenolpyruvate carboxykinase[PEPCK], glucose-6-phosphatase[G6Pase]) and on adipocyte differentiation in H4IIE and 3T3-L1 cells, respectively, were examined. CK suppressed the induction of PEPCK and G6Pase mRNA expressions under the dexamethasone/cAMP stimulation condition. CK also reduced the PPAR-γ mRNA expression and triglyceride accumulation in a dose dependent manner as compared to the control. The present study suggests that CK deserves to examine whether it shows an anti-diabetic activity in animal and human studies.

대학생의 성희롱·성폭력 실태에 관한 연구

박금주,김두화,김성현 濟州大學校 學生生活硏究所 2005 學生生活硏究 Vol.26 No.-

The purpose of this research is to understand the sex culture of college students by surveying the students' awareness and experience about sexual harassment and sexual assault, and their responses and opinions, and ultimately to provide basic data for in-campus programs and guidance against sexual harassment and assaults. To this end, the survey was composed of three parts; awareness on sexual harassment and sexual assaults, experience of sexual harassment or assault, and opinions on the consultation program on harassment and sexual assaults. The survey was conducted among 1,000 students in 9 colleges of Cheju National University (CNU), and analyzed the answers of 964 respondents. This research used SPSS statistical program to process data and conducted t-verification and F-verification. The results of the research are as follows. 1) The awareness on sexual harassment and assaults CNU students' common notions on sexual assaults, attitudes toward sexual relations, views on sexual harassment are a little different from the generally accepted ones, but lower than socially expected. In general, male students have more stereotypes on these matter sand take them less seriously than female students. Both of them have a man-oriented attitude. While female students take sexual harassment and sexual assaults as social problems, male students just take them as flirting. 2) Experience of sexual harassment and assaults After conducting a survey with three cases; committing experience, suffering experience, and experience of hearing of or seeing, there is no case in committing experience. In case of suffering sexual harassment or assaults, verbal, nonverbal, and physical cases are answered in order. In case of verbal harassment, female and male students suffer similarly, while more male students experience nonverbal, physical harassment and assaults, which shows that sexual harassment and assaults are happening irrespective sex. As to inflictors, strangers are the most. Sexual harassment and assaults occur more in other places rather than in campus, while in-campus cases are relatively less answered. In-campus cases are mostly between students, but there are cases where a staff member or a professor is an assailant, though very rare. About the reaction to sexual harassment and assaults, the highest number of students, male and female, answered they say "No" and ask to stop. Their reactions seem to be active and desirable. Most of them talk with their friends about the problem. 3) Opinion on the sexual harassment and assaults consultation center The level of awareness of CNU students about the center's regulations on sexual harassment and assaults cases is very low; therefore they said the center should give the priority to education for sexual assaults prevention. - As equally important as to resolve the cases of sexual harassment and assaults, there should be efforts to build a common perception on sexual harassment and assaults. To this end, education and discussion programs should be activated and continued to give students a right understanding of this issue. - In-campus sexual harassment and assaults are mostly between students, but there are cases where a staff member or a professor is an assailant, though very rare. Since such a case, if happening, is related to officials' duty and authority, it should be completely prevented. - Education program for the prevention of sexual harassment and assaults would be desirable to conduct in a small group consisting of friends or peers. - It is needed to widely inform members of the university about the regulations on sexual harassment and assaults. In this regard, compulsory and regular education should be offered to all the CNU students rather than one time campaign. - It would be desirable for the center to conduct all-year programs for the prevention of sexual harassment and assaults.