남은 음식물 발효사료 급여가 도체 특성 및 돈육품질에 미치는 영향

박근규,박홍양,정영철,이의수,양시용,임병순,김천제,Park, Keun-Kyu,Park, Hong-Yang,Jung, Young-Chul,Lee, Eui-Soo,Yang, Si-Yong,Im, Byoung-Soon,Kim, Cheon-Jei 한국식품과학회 2005 한국식품과학회지 Vol.37 No.1

본 실험은 돈육의 도체성적과 육질을 비교함으로써 남은 음식물 발효사료 급여효과를 살펴보고자 실시하였다. 도체성적으로 생체중, 도체중, 도체율, 등지방두께, 도체등급, 부분육량 및 정육율을 측정하였으며, 육질평가로 pH, 보수력, 저장감량, 가열감량, 전단력, 육색 및 NPPC 육색 및 근내지방도를 측정하였다. 도체특성에 있어서 등지방두께를 제외하고 도체중, 지육율 및 최종등급은 대조구와 FFWF구간에 유의적인 차이가 없었으며, 부분육 생산량과 정육율에 있어서는 FFWF구가 대조구에 비하여 다소 높게 나타났다. 육질특성에 있어서는 pH는 FFWF구가 대조구에 비하여 다소 낮게 나타났으나, 보수력, 드립감량, 가열감량, 전단력 등은 대조구와 FFWF구간에 유의적인 차이가 없었다. 육색에 있어서는 FFWF구가 대조구에 비하여 L*-값이 높고 a*-값이 다소 낮게 나타났으며, 주관적인 방법으로 측정된 NPPC 육색과 근내지방도는 처리구간에 차이가 없었다 따라서 본 연구의 결과로 볼 때 남은 음식물 발효사료의 급여가 일반배합사료 급여와 비교하여 도체 및 육질 특성에 있어서 크게 떨어지지 않는 것으로 판단되었다. Effects of fermented food waste feeds on meat quality and physico-chemical characteristics of fully grown pigs were investigated. Two treatments were tried in this study; commercial feed (control) and fermented food waste feed(FFWF). Except for much thicker backfat of control, carcass traits, carcass weight, dressing percentage, carcass grade, cooking loss, shear force, water-holding capacity, drip loss, NPPC meat color, and marbling score of commercial feed(control)- and fermented food waste feed (FFWF)-fed pigs were not significantly different. Primal cut weights and meat percentages were significantly different (p<0.05) between control and FFWF. pH of FFWF loin was significantly lower (p<0.05) than that of control. FFWF loin showed higher L*-value and lower a*-value compared to control.

이상지혈증

박근규 ( Keun Gyu Park ) 대한간학회 2013 Postgraduate Courses (PG) Vol.2013 No.-

Patients with type 2 diabetes have high rates of cardiovascular disease (CVD), much of which may be preventable with appropriate treatment of lipid abnormalities. Diabetic dyslipidemia most commonly manifests as elevated triglycerides and low levels of HDL cholesterol, with a predominance of small, dense LDL particles amid relatively normal LDL cholesterol levels. Elevated serum triglycerides commonly associate with insulin resistance. In diabetic patients, despite the insulin resistance of the gluconeogenic arm, persistently elevated hepatic lipogenesis in response to hyperinsulinemia could neatly account for the increased hepatic triglyceride output. Insulin`s ability to activate lipogenesis is mediated by both transcriptional and post-translational activation of SREBP-1c. Recently, we demonstrated that fenofibrate, which is a drug used to treat hypertriglyceridemia, decreases hyperinsulinemia-stimulated hepatic lipid synthesis through induction of ER membrane bound transcription factor, CREBH. In the study, we also found that fenofibrate and CREBH induce hepatic fibroblast growth factor 21 (FGF21) expression. FGF21 has emerged as an important metabolic regulator of glucose and lipid metabolism. In this symposium, I will review diabetic dyslipidemia and present the data showing positive regulators of hepatic FGF21 expression.

남은 음식물 발효사료 급여가 돈육 삼겹살의 소비자 기호도에 미치는 영향

박홍양,박근규,정영철,이의수,양시용,임병순,김천제,Park Hong-Yang,Park Keun-Kyu,Jung Young-Chul,Lee Eui-Soo,Yang Si-Yong,Im Byoung-Soon,Kim Cheon-Jei 한국축산식품학회 2004 한국축산식품학회지 Vol.24 No.4

본 연구는 남은 음식물 발효사료 급여가 돈육의 소비자 기호도에 미치는 효과를 알아보고자 국내에서 가장 소비가 많이 되고 있는 삼겹살 부위를 이용하여 일반소비자를 대상으로 기호도 조사를 실시하였다. 암퇘지 삼겹살의 경우 생육에 대한 소비자 평가는 신선도를 제외하고는 대조구와 시험구(FFWF) 사이에 뚜렷한 차이가 없는 것으로 나타났으며, 시식 후 소비자 관능특성에 있어서도 대조구와 시험구(FFWF) 사이 에 차이 가 없었다. 거세돈 삼겹살의 경우 생육에 대한 소비자 평가는 지방량을 제외하고 대조구와 시험구(FFWF) 사이에 뚜렷한 차이가 없는 것으로 나타났으며, 시식 후 소비자 기호도도 대조구와 시험구(FFWF) 사이에 차이가 없는 것으로 평가되었다. 전체적인 생육에 대한 소비자 평가는 육색을 제외하고는 대조구와 시험구(FFWF) 사이에 차이가 없는 것으로 나타났으며, 시식 후의 소비자 관능특성에 있어서도 대조구와 시험구(FFWF)는 차이가 없는 것으로 평가되었다. 선호도에 있어서는 암퇘지의 경우 대조구가 시험구(FFWF)에 비하여 선호도가 높게 나타났으며, 거세돈의 경우 시식 전 생육에 있어서는 시험구(FFWF)가 대조구에 비하여 선호도가 높게 나타났으나, 시식후의 선호도에 있어서는 대조구와 시험구(FFWF) 사이에 차이가 없었다. 전체적인 두 처리구 간의 선호도는 생육과 시식 후 모두 대조구와 시험구(FFWF) 사이에 차이가 없었다. 이상의 돼지 삼겹살의 소비자 기호도 평가 결과를 종합해 볼 때 남은 음식물 발효사료의 돼지사료로 이용 가능성을 확인하였다. The objective of this experiment was to compare tile effect of feeding the commercial feeds (control) or fermented food waste feeds (FEWF) on consumer's acceptability and preference of pork belly. The bellies from carcasses of gilts and barrows were used. The consumers evaluated raw meat for color, freshness, fat amount (5=too much fat; 1= not enough fat), fat acceptability and overall acceptability on 5-point stale (5=most desirable; 3=moderate; 1=least desirable) and grilled belly for flavor, taste, texture, juiciness and overall acceptability on 5-point scale (5=most desirable; 3=moderate: 1=least desirable). Raw belly of control had higher scores in color than belly fed FFWF. However, there were no differences between treatments for freshness, fat contents, acceptability of fat contents and overall acceptability (p>0.05). There were no differences in any sensory trait between control and FFWF belly after cooking (p>0.05). Mean scores for preference or overall-liking of raw and cooked belly also were not significantly different between two groups (p>0.05). This results mean that feeding FFWF to pork didn't change the sensory Properties of belly meat.

Ovotransferrin 가수분해물의 Angiotensin-converting Enzyme 활성억제 효과 및 생산 최적화

이나경,안동욱,박근규,백현동,Lee, Na-Kyoung,Ahn, Dong-Uk,Park, Keun-Kyu,Paik, Hyun-Dong 한국축산식품학회 2010 한국축산식품학회지 Vol.30 No.2

Angiotensin-converting enzyme (ACE) inhibitory activity and production optimization of ovotransferrin hydrolysates were studied. Ovotransferrin was hydrolyzed by several enzymes (protamex, alcalase, trypsin, pepsin, neutrase, and flavorzyme) and acid (0.03 N HCl). Ovotransferrin hydrolysate reduced ACE activity by 60.2%, 55.8%, and 42.6% when treated with trypsin, acid, and pepsin, respectively. Trypsin was selected for production of peptide having maximum AC inhibitory effect, which was greatest with 7 h hydrolysis. Central composite design determined that optimum composition of ACE inhibitory substances using substrate concentration of 20-35%, temperature of $35-55^{\circ}C$, and pH of 6.0-8.0. The optimum composition was 1% trypsin, substrate concentration of 26.32%, $51.29^{\circ}C$, and pH 6.32. Under this conditions, a maximum ACE inhibitory effect of 69.1% was evident, similar to the predicted value.

부산물사료 주원료 사일리지(Biosilage^®) 급여가 거세 육성 한우의 행동양식에 미치는 영향

김영일,이상무,박근규,곽완섭,Kim, Young Il,Lee, Sang Moo,Park, Keun Kyu,Kwak, Wan Sup 한국초지조사료학회 2013 한국초지조사료학회지 Vol.33 No.4

This study was carried out to investigate the effects of a by-product feed-based silage (BF silage) feeding on behavior patterns of growing Hanwoo steers. A total of 10 Hanwoo steers (11 months old, 302kg of body weight) were assigned to 2 dietary treatments: the control (concentrate mix + free access to rice straw), and the treatment (concentrate mix + free access to BF silage). The behavior patterns were observed for 48 hours. The intakes of dry matter (DM) and neutral detergent fiber (NDF) of the treatment group were higher than those of the control group. Eating time, ruminating time and resting time were not different between the control and treatment. But, the intake time per kg DM was higher for the control than treatment. The number of bolus, total chewing frequency, number of ruminating per bolus and number of bolus per minute were not different between the control and treatment. But the chewing frequency per bolus was higher in the treatment than control (p<0.05), and feed value index was lower in treatment than control (p<0.05). Frequencies of drinking and defecating were not different between the two groups, but the frequency of urinating was higher for the treatment (p<0.05) than control. Eating rate, ruminating efficiency and chewing efficiency were much higher in the treatment group than control (p<0.05). These results indicate that the replacement of conventional rice straws with the BF silage (physically effective NDF, about 25%) did not affect the ruminating behaviors of Hanwoo steers significantly. 본 연구는 버섯재배부산물, 재활용가금깔개, 미강 및 straw로 구성되는 BF 사일리지 급여가 육성 거세한우의 행동양상에 미치는 영향을 규명하기 위하여 실시하였다. 시험에 사용한 공시 한우는 11개월령 총 10두(평균 301.7 kg)를 2처리로 배치하였으며, 이 때 대조구(볏짚 자유채식)와 처리구(BF silage 자유채식)로 하여 48시간 행동관찰을 실시하였다. 대조구와 비교해서 BF 사일리지 급여구는 총 DM 섭취량과 NDF 섭취량이 각각 30% 및 36% 높았고, 채식시간, 반추시간 및 저작시간은 유의적인 차이가 없었다. 식괴수, 식괴당 반추시간에 있어서도 유의적 차이가 없었고, 식괴당 저작수와 FVI는 낮게 나타났다(p<0.05). 또한 대조구와 비교해서 BF 사일리지 급여구는 배뇨 회수가 높고(p<0.05), 음수 및 배분 회수에 있어서는 상호간에 차이가 없었고, 채식율, 반추효율 및 저작효율에 있어서는 더 높게 나타났다(p<0.05). 이상의 결과로 볼 때, BF 사일리지 급여는 볏짚과 비교 시 육성거세한우의 반추행동 상 큰 차이가 없다는 결과로 미루어 볼 때 차후 볏짚 대용으로 활용하여도 좋을 것으로 사료되었다. 즉 입자도가 큰 straw를 peNDF를 충족시키는 최소량을 혼합하여 부산물사료 사일리지를 제조, 급여하였을 때 육성거세한우의 반추행동은 볏짚 급여 시와 차이가 없었다.

Hetero 발효 유산균주 첨가가 사일리지용 이탈리안 라이그라스와 총체보리의 품질에 미치는 효과

이호일,최연재,로벨리아 마무아드,김은중,오영균,박근규,이상석,Lee, Ho Il,Choi, Yeon Jae,Mamuad, Lovelia,Kim, Eun Joong,Oh, Young Kyoon,Park, Keun Kyu,Lee, Sang Suk 한국초지조사료학회 2014 한국초지조사료학회지 Vol.34 No.4

본 연구에서는 hetero 발효유산균 첨가가 이탈리안 라이그라스 및 총체보리 사일리지의 사료가치 및 발효품질에 미치는 영향을 조사하였다. 사일리지 접종균주를 선발하기 위해 유산균 6종(L. plantarum IMAU 70164, L. acidophilus KACC 12419, L. casei KACC 12413, L. reuteri KCTC 3594, L. buchneri KACC 12416 and L. diolivorans KACC 12385)을 이용하였으며, 젖산 함량이 높은 L. casei KACC 12416와 프로피온산 함량이 높은 L. reuteri KCTC 3594을 최종 선택하여 사일리지를 제조하였다. 처리구는 대조구, L. casei 첨가구, L. reuteri 첨가구 및 L. casei, L. reuteri 혼합첨가구로 하여 30일, 45일 및 60일간 저장하였다. pH는 IRG 사일리지의 경우 유산균 첨가구가 대조구에 비해 낮았으며(p<0.05), 수분함량, 젖산 및 초산함량 또한 유산균을 첨가한 처리구에서 낮은 것으로 나타났다(p<0.05). 수용성 탄수화물의 함량은 L. casei KACC 12416와 L. reuteri KCTC 3594를 혼합배양한 처리구에서 가장 낮았다(p<0.05). 프로피온산은 유산균을 접종한 총체보리에서 전 기간동안 높게 나타났으며, 반면에 사일리지 품질을 저해시키는 butyrate는 발효 60일의 대조구에서 측정되었다. 사일리지의 미생물 측정결과 IRG 사일리지 배양 60일에 유산균 첨가구에서 곰팡이가 억제되었으며, WCB 사일리지는 유산균을 첨가한 처리구에서 곰팡이가 검출되지 않았다. 이상의 연구 결과들로 볼 때, 단일 균주를 접종한 사일리지에 비해 co-culture (L. casei + L. reuteri)로 접종했을 때 사일리지 저장품질 개선에 보다 효과적이었으며, 혼합배양한 처리구가 청보리 사일리지내 높은 프로피온산을 생성하며 이는 사일리지의 품질개선에 도움을 주는 것으로 조사되었다. This study was conducted to determine the quality of italian ryegrass (IRG) and whole- crop barley (WCB) silage combined with heterofermentative lactic acid bacteria (LAB) during fermentation. Six strains of LAB (L. plantarum IMAU 70164, L. acidophilus KACC 12419, L. casei KACC 12413, L. reuteri KCTC 3594, L. buchneri KACC 12416 and L. diolivorans KACC 12385) were used in this study. L. casei and L. reuteri had the highest propionic acid production and were therefore used for fermenting the forages. The forages were fermented using monoculture and co-culture of L. casei and L. reuteri for 30, 45 and 60 days of ensiling. Addition of LAB lowered the pH of the IRG silage (p<0.05). Moisture content, lactic acid and acetic acid contents were higher (p<0.05) after addition of LAB. Water soluble carbohydrate was significantly lower (p<0.05) in WCB with a co-culture containing L. casei and L. reuteri. Propionic acid production was comparatively higher after addition of LAB to WCB on days 30, 45 and 60 while butyric acid was only detected in the IRG control on day 60. Fungi was not detected within 60 days after addition of LAB in IRG and WCB. Through this experiment, improved forage preservation was achieved using a co-culture containing L. casei and L. reuteri. WCB silage had higher propionic acid concentration and thus, it was a better forage for ensiling using co-culture of L. casei and L. reuteri.

엿밥의 조성 및 산유량과 유성분 조성에 미치는 영향

양시용,강창원,안규철,이강연,이중일,김정훈,박근규,Yang, Si-Yong,Kang, Chang-Won,Ahn, Gyu-Chul,Lee, Gang-Yeon,Lee, Jung-Il,Kim, Jeong-Hoon,Park, Keun-Kyu 한국축산식품학회 2010 한국축산식품학회지 Vol.30 No.3

This study was conducted to the evaluate nutritional value of corn taffy residue (CTR), a by-product from Korean traditional taffy, and to investigate the dietary effect of CTR on the yield and composition of milk from dairy cows. Relatively high concentrations of residual enzymes and sugars were found in CTR. Protein and fat concentrations were high, comparable to those of corn gluten meal. Inclusion of 1, 3, and 6% CTR in total mixed rations yielded more milk (p<0.05) than in the control group, and increased milk fat (p<0.05) in high-producing cows (approximately 30 kg). In low-producing cows, (approximately 20 kg), milk yield was increased by 1 and 6% CTR, and milk protein was enhanced by 3% CTR (p<0.05). Based on these results, utilization of CTR is recommended for production of high quality and economical animal products.

증례 : 갑상선기능항진증 환자에서 propylthiouracil 복용 후 발생한 typhlitis 1예

류성열 ( Seong Yeol Ryu ),장영윤 ( Young Yun Jang ),김상윤 ( Sang Yoon Kim ),박근규 ( Keun Gyu Park ),김혜순 ( Hye Soon Kim ) 대한내과학회 2007 대한내과학회지 Vol.73 No.6

Typhlitis은 주로 급성 백혈병으로 항암 치료 후 호중구 감소증 상태에서 발생하는 대장염을 말하는데, 간혹 골수 이식 후 면역억제재 치료 또는 약물에 의한 무과립구증, AIDS, 고형암의 화학요법 후에도 발생할 수 있다. 저자들은 갑상선기능항진증 치료에 사용되는 propylthiouracil을 복용한 후 호중구 감소 상태에서 발생한 Typhlitis 1예를 경험하였으므로 문헌고찰과 함께 보고하는 바이다. 드물지만 갑상선기능항진증 환자에서 propylthiouracil 투여 후 복통, 설사와 발열 등의 증상이 동반되면 typhlitis의 가능성을 고려하여 조기에 적절한 진단과 치료를 하여야 할 것으로 사료된다. Typhlitis is one of the most ominous complications in immunocompromised patients. Neutropenic enterocolitis or typhlitis is a clinical syndrome characterized by fever, diarrhea and abdominal pain that occurs in neutropenic patients. It has been reported as a complication of childhood leukemia, but is now known to occur in adults with solid malignancies, acquired immunodeficiency syndrome (AIDS) or bone marrow transplantation (BMT). The association of typhlitis and propylthiouracil has not been previously reported. We report a case of a 42-year-old female patient with typhlitis due to propylthiouracil patient with hyperthyroidism. (Korean J Med 73:666-669, 2007)

갑상선 유두암에서 p16(INK4A) 촉진자 과메틸화와 p16(INK4A), cyclin D1 및 Rb 발현

김윤정 ( Yoon Jung Kim ),김혜순 ( Hye Soon Kim ),하유진 ( Yu Jin Ha ),이호영 ( Ho Young Lee ),박근규 ( Keun Gyu Park ),김미경 ( Mi Kyung Kim ),권선영 ( Sun Young Kwon ) 대한내과학회 2010 대한내과학회지 Vol.78 No.3

Background/Aims: Dysregulation of the cell cycle is believed to be important in human neoplasia. p16(INK4a), a regulator of the retinoblastoma protein, is inactivated by several mechanisms, including hypermethylation of the promoter. The aim of this study was to assess the relationship between p16(INK4a) methylation status and the expression of p16(INK4a), cyclin D1, and retinoblastoma protein in papillary thyroid cancer. Methods: Thirty-five surgically resected papillary thyroid cancer cases treated at Keimyung University Dongsan Medical Center from Jan 2003 to Dec 2005 were included in the study. We examined promoter hypermethylation of p16(INK4a) and immunohistochemically analyzed p16(INK4a), cyclin D1, and retinoblastoma protein expression. Results: Aberrant hypermethylation of 5` CpG islands of the p16(INK4a) gene promoter was observed in 17 (48.6%) out of 35 cases, and the p16(INK4a) protein was lost in 18 (51.4%) cases. With the exception of one case, p16(INK4a) promoter hypermethylation correlated with the loss of p16(INK4a) protein expression (p<0.0001). Overexpression of cyclin D1 was found in 27 (77.1%) cases and retinoblastoma immunostaining was detected in 10 (28.6%) cases. There was an inverse relationship between p16(INK4a) and pRb immunostaining (p<0.018, r=-0.398), and a strong direct correlation between cyclin D1 and pRb immunostaining (p<0.0001, r=0.710). Cyclin D1 expression was significantly associated with the pathologic T stage of cancer (p=0.04). Conclusions: These data suggest that promoter hypermethylation is a major mechanism underlying the inactivation of p16(INK4a) and alterations in the p16(INK4a)/cyclin D1/retinoblastoma pathway, which are thought to be very important in papillary thyroid cancer. (Korean J Med 78:333-340, 2010)

불명열의 원인으로 제시된 아급성 갑상선염의 증례

김종호 ( Jong Ho Kim ),배귀현 ( Kwi Hyun Bae ),최연경 ( Yeon Kyung Choi ),하인균 ( In Gyoon Ha ),박근규 ( Keun Gyu Park ),김정국 ( Jung Guk Kim ),이인규 ( In Kyu Lee ) 대한내과학회 2013 대한내과학회지 Vol.84 No.5

Fever of unknown origin (FUO) is not infrequently a diagnostic dilemma for clinicians. Common infectious causes include endo-carditis and abscesses in adults, and noninfectious causes include neoplasms and certain collagen vascular diseases. Endocrine causes of FUO are rare. The only endocrine disorder likely to present as FUO is subacute thyroiditis. Subacute thyroiditis usually occurs in middle-aged women as viral prodrome, neck tenderness, classic symptoms of thyrotoxicosis, and an elevated erythrocyte sedimentation rate. The patient may have abrupt onset of fever and chills with complaints of thyroid pain, or only low-grade fever with poorly characterized anterior neck pain. We present a case of FUO in a 48-year-old female who had had fever and neck pain for more than one month. Despite an extensive evaluation, the patient had persistent fever and no cause was found, with the exception of subacute thyroiditis. The fever resolved from the second day of treatment with low-dose steroid (prednisolone, 10 mg per day). This case illustrates that subacute thyroiditis should be considered in cases of FUO. (Korean J Med 2013;84:733-736)