미토콘드리아와 Cytochrome c 결합에 미치는 염기성 화합물의 영향

박광숙,Suhas Desi,박인국,전길자,김상덕,백운기 ( Kwang Sook Park,Suhas Desi,In Kook Park,Gil Ja Jun,Sang Duk Kim,Woon Ki Paik ) 생화학분자생물학회 1987 BMB Reports Vol.20 No.2

Binding of horse heart (methyl-^(14)C)cytochrome c reductively methylated with (^(14)C) formaldehyde to isolated rat liver mitochondria has been investigated. The number of binding sites is calculated to be 56 pmoles of cytochrome c/㎎ of mitochondria) protein, and the affinity constant (K_a) to be 1.79 × 107M^(-1). Various naturally occurring basic compounds including histones, protamine and polyamines are highly inhibitory on the (methyl-^(14)C)cytochrome c binding. Almost all of (methyl-^(14)C)cytochrome c bound to mitochondria can be released from the mitochondria by subsequent treatment with nonlabeled cytochrome c. Although histone H3 (arginine-rich histone) has much stronger inhibitory effect on the (methyl-^(14)C)cytochrome c binding than non-labeled cytochrome c at equimolar concentration when present in the binding assay mixture, only a fraction of bound (methyl-^(14)C)cytochrome c can be freed from mitochondria by treatment with histone H3. Evidence indicates that these effect are not merely a consequence of electrostatic influence on the cytochorme c receptor of mitochondria.

Effect of Naturally Occurring Basic Compounds on the Cytochrome C Binding to Mitochondria

박광숙,박인국,전길자,김상덕,백운기,Park, Kwang-Sook,Desi, Suhas,Park, In-Kook,Jun, Gil-Ja,Kim, Sang-Duk,Paik, Woon-Ki Korean Society for Biochemistry and Molecular Biol 1987 한국생화학회지 Vol.20 No.2

목포지역에서의 B 형 간염 (肝炎) 바이러스 감염요인에 (感染要因) 관한 역학적 연구

박광숙 ( Kwang Sook Park ),김지운 ( Ji Woon Kim ),최관수 ( Gwan Su Choi ),정원재 ( Won Jae Jeong ),정남기 ( nam Ki Joung ) 대한소화기학회 1985 대한소화기학회지 Vol.17 No.2

N/A We tested 1389 pregnant women for HBsAg and 197 pregnant women for anti-HBs, anti-HBc, HBeAg and antiHBe with EIA method. 1) The occurance rate of HBsAg was 8.8Yo in a total of 1389 cases. 2) The occurance rate of HBsAg was unrelated with age, because it was the lowest in the age group of 21-30 years(7.9%) and much higher in the age group of below 20 years (16.0%) and over 30 years(15.3%). 3) The occurance rate of HBsAg was related a little with locality. It was slightly higher in cases from the city(9.3% than in cases from the village(8.5%). 4) The occurance rate of HBsAg was unrelated to educational background. 5) The occurance rate of HBsAg was increased in groups with an income of 400,000-500000 won(10.3%) and over 500,000 won(13.1%) than in groups with smaller income. 6) The effect of family history of liver disease: The occurance rates of all hepatitis B viral markers were increased in those who had a family history of liver disease, but the occurance rates of HBsAg(p<0.005%, antiHBs(p<0.005) and antiHBc(0.025<p<0.01) were increaed significantly. 7) The effect of acupuncture: The occurance rates of HBsAg and HBeAg were increased significantly in those who had a history of acupuncture(p<0.005, 0.025<p< 0.01), but te occurance rates of anti-HBs and antiHBe were decreased. 8) The occurance rate of antiHBs was increased significantly in student nurses(p. 6i) and graduate nurses(58.0%) compared to university students(27.7%)(p<0.005). The occurance rate of anti-HBc was increased but not significantly(0.05<p<0.1), but there was no Significant difference between each year by duration of exposure in hospital work. The occurance rate of HBsAg was not related in the three groups.

소 간증의 Ornithine Aminotransferase 의 특성

박광숙 ( Kwang Sook Park ) 생화학분자생물학회 1985 BMB Reports Vol.18 No.4

Bovine liver ornithine aminotransferase (L-ornithine: 2-oxoacid aminotransferase, EC 2.6.1.13) was purified as high as 209 fold (6.4% yield) and its molecular weight was estimated to be 88,000 by the method of gel filtration. Two dimensional combined isoelectric focusing-SDS polyacrylamide gel electrophoresis revealed a single band with an apparent molecular weight of 42,000, suggesting that the enzyme consists of two subunits. pI of the enzyme was 5.33 and its optimum pH was found to be 8.0. The enzyme contained approximately one mole of pyridoxal phosphate per mole and the total number of thiol groups per mole was estimated to be nine. K_m`s of the enzyme for L-ornithine and α-ketoglutarate were determined to be 5.78 mM and 0.5 mM respectively. L-Valine was competitive with L-ornithine and its K_i and found to be 9.5 mM. v

간장 및 담도 : 산모와 신생아에서 B 형 간염표식자에 (肝炎標識子) 관한 연구

박광숙(Kwang Sook Park),최관수(Gwan Su Choi),정원재(Won Jae Jeong),김지운(Ji Woon Kim),정남기(nam Ki Joung),장영호(Yung Ho Chang) 대한소화기학회 1986 대한소화기학회지 Vol.18 No.1

N/A To analyze frequency and severity of liver disease in 3rd trimester and full term Korean pregnant women, to observe the prevalence of HBV markers in pregnant women and newborn infants at delivery and to evaluate the effect of HBV on fetus, newborn infants and pregnant women. We received the findings of the physical examination and history taking, and did SGPT and HBV markers with EIA method at intervals in 2,873 pregnant women who delivered at St. Columbans Hospital in Mokpo City from April 1st, 1985 to March 31st, 1986. The following results were obtained: 1) The overall HBsAg positivity in all 2,873 pregnant women was 8.3% 2) Among 2,873 pregnant women, 5 cases in 3rd trimester and 6 cases in full-term pregnant women showed typical acute viral hepatitis B and most of them were normal in their follow-up tests. 32 cases who shoWed mild elevation of SGPT were normal in their follow-up tests. But it was difficult to differentiate between a course of acute or chronic hepatitis and mild liver dysfunctions during pregnancy. 3) Among 195 HBsAg positive pregnant women, 76 cases(39.0%) were HBeAg positive, 65 cases(33.3%) were HBeAg negative and 54 cases(27.7%) were Anti-HBe positive. 4) Mother to infant transmission of HBV markers at delivery was as follows: Positive rate of HBsAg was 10.0% or 14 infants in 140 pregnant women. Positive rate of HBeAg was 17.8% or 8 infants in 45 cases. Positive rate of anti-HBs was 88.9% or 16 infants in 18 cases. Positive rate of anti-HBc was 96.2% or 125 infants in 135 cases. Positive rate of anti-HBe was 100% or 35 infants in 35 cases. 5)We evaluated the HBV markers in newborn infants 3 groups according to the maternal HBeAg/Anti-HBe status at delivery in 107 HBsAg positive pregnant women. Out of 38 HBeAg positive women, 6 infants(15.8%) were positive for HBsAg and HBeAg, 31 infants (81.6%) were negative for HBsAg, HBeAg and anti-HBe. Out of 39 HBeAg reative women, 2 infants(5.1%) were positive for HBsAg and negative for HBeAg, 31 infants(79.5%) were negative for HBsAg, HBeAg and anti-HBe. Out of 30 anti-HBe positive women, 3 infants (10.0%) were positive for HBsAg and anti-HBe, negative for HBeAg, 22 infants(73.3%) were negative for HBsAg and HBeAg, positive for anti-HBe. HBV markers in newborn infants corresponded to their mothers degree of infection. 6) Among 2,873 pregnant women, vve found 135 cases of prematurity, 6 cases of still birth cases of intrauterine death, 56 cases of congenital anomaly and 122 cases of severe physiologic jaundice. We also found in the past history 75 cases of prematurity, 18 cases of stillbirth, 36 cases of intrauterine death, 14 cases of congenital anoma!y, 34 cases of infantile death and 444 cases of spontaneous abortion. There was no significant incrcase in frequency of adverse effects of HBV according to the mothers HBsAg positivity status. 7) Positive rate of HBsAg was 16.4% in 61 cases who showed toxemia of pregnancy. Positive rate of HBsAg was 8.1%. In 2, 812 cases who showed no toxemia of pregnancy. There was significant increase of toxemia of pregnancy in HBsAg carrier(0.01<P<0.025). Positive rate of HBsAg was 14.7% in 109 cases who bled more than 500 cc postpartum hemorrhage. Positive rate of HBsAg was 8.1% in 2812 cases who bled less than 500cc postpartum hemorrhage. There was significant increase of postpartum hemorrhage in HBsAg carrier(0.01<p<0.005). Severity of hyperemesis was unrelated to HBsAg positivity. 8) We observed the course and severity of hepatitis during pregnancy. Among 5 cases in 3rd trimester and 6 cases in full term pregnant women, only one case showed elevation of SGPT for more than 6 months. Among 19 non-pregnant women, one case in 15 patients of acute viral hepatitis and 3 cases in 4 patients of chronic viral hepatitis showed elevation of SGPT for more than 6 months. There was no significant difference in couse, severity and prevalence of HBV markers between pregnant and non-pregnant women.

거핵구 특이 유전자 혈소판 인자 4 에 대한 분자생물학적 이해

박광숙 ( Kwang Sook Park ) 생화학분자생물학회 1992 생화학총설집 Vol.4 No.-

닭 간의 Ornithine Aminotransferase 에 관한 연구

김경순,박종옥,박광숙 ( Gyung Soon Kim,Jong Ok Park,Kwang Sook Park ) 생화학분자생물학회 1985 BMB Reports Vol.18 No.2

Ornithine aminotransferase was purified from chicken liver and several properties of the enzyme were studied. The purified enzyme appeared to be homogeneous on polyacrylamide gel electrophoresis with and without SDS. The molecular weight of the enzyme was estimated as 62,000 by SDS-polyacrylamide gel electrophoresis, and as 63,000 by gel filtration. The K_m values for ornithine and α-ketoglutarate were found to be 6.31 mM and 1.03 mM, respectively. The enzyme was inhibited either by high substrate concentrations or by p-chloromercuriphenylsulfonic acid. Glyoxylate, oxaloacetate and pyruvate could act as amino group acceptors, but they were much less effective than α-ketoglutarate. Optimum pH of this enzyme was found to be 7.3.

Studies on the Ornithine Aminotransferase from Chicken Liver

김경순,박종옥,박광숙,Kim, Gyung-Soon,Park, Jong-Ok,Park, Kwang-Sook 생화학분자생물학회 1985 한국생화학회지 Vol.18 No.2

닭 간에서 ornitine aminotransferase는 주로 mitochondrial fraction에 존재하였다. 이 효소를 열처리, ammonium sulfate fractionation, DEAE-cellulose ion exchange chromatography, Sephadex G-200 filtration, CM-Sephadex column chromatography를 이용하여 정제해서 disc polyacrylamide gel electrophoresis를 하였더니 하나의 단백질 띠가 얻어졌다. 이 정제된 효소를 이용해서 몇가지 성질을 조사해본 결과는 다음과 갈다. SDS-polyacrylamide gel electrophoresis에 의해 측정된 분자량은 62,000이었고 gel filtration에 의한 값은 63,000이었으므로 이 효소는 monomer로 존재한다고 생각되었다. L-ornithine과 $\alpha$-ketoglutarate에 대한 $K_m$ 값은 각각 6.31 mM, 1, 03 mM 이였으며, 이 효소는 기질의 농도가 높을 때 저해되었고 또한 3 mM p-chloromercuriphenylsulfonic acid를 가해주면 80% 저해되었다. glyoxylate, oxaloacetate, pyruvate도 L-ornithine보다는 못하지만 amino group acceptor로서 작용하였으며, 효소활성에 대한 최적 pH는 7.3으로 측정되었다. Ornithine aminotransferase was purified from chicken liver and several properties of the enzyme were studied. The purified enzyme appeared to be homogeneous on polyacrylamide gel electrophoresis with and without SDS. The molecular weight of the enzyme was estimated as 62,000 by SDS-polyacrylamide gel electrophoresis, and as 63,000 by gel filtration. The $K_m$ values for ornithine and $\alpha$-ketoglutarate were found to be 6.31 mM and 1.03 mM, respectively. The enzyme was inhibited either by high substrate concentrations or by p-chloromercuriphenylsulfonic acid. Glyoxylate, oxaloacetate and pyruvate could act as amino group acceptors, but they were much less effective than $\alpha$-ketoglutarate. Optimum pH of this enzyme was found to be 7.3.

위내시경으로 (胃內視鏡) 진단된 각종 위질환 (胃疾患) 에서의 장상피화생의 (腸上皮化生) 유형에 관한 연구

윤종만(Chong Mann Yoon),박광숙(Kwang Sook Park),범희승(Hee Seung Bom),박일종(Il Chong Park),류종선(Jong Sun Rew),양대현(Dae Hyun Yang),조정곤(Jung Kon Cho) 대한소화기학회 1987 대한소화기학회지 Vol.19 No.1

N/A To evaluate the significance of intestinal metaplasia as a guide for precancerous lesions in gastric cancer, we studied the frequency and subtypes of intestinal metaplasia in 189 patients including 64 cases of chronic gastritis, 45 with gastric ulcers and 80 with gastric cancer who underwent gastroscopic biopsies in the department of internal medicine of Chonnam Univerity Hospital. The results were the following: 1) The overall positivity of intestinal metaplasia in 189 patients with various gastric lesions was 57 percents with 47 percents in chronic gastritis, 62 percents in gastric ulcer and 61 percents in gastric cancer. 2) The positivity of intestinal metaplasia in gastric cancer in well differentiated types (69%) was not so higher than in undifferentiated types (48%). But the positivity of intestinal metaplasia in gastric cancer of intestinal type (84%) was higher than in gastric type (21%) with statistical significance (p<0.005). 3) The frequency of intestinal metaplasia showed increasing tendency with aging. 4) According to the Hematoxylin-Eosin, PAS and Alcian-Blue stain, intestinal metaplasia is divided into 3 subtypes. Type II enterocolic metaplasia is not detected in chronic gastritis but in only one case of gastric ulcer. The presence of enterocolic metaplasia is 20 out of 49 patients with gastric cancer, with 44 percents in intestinal type and 0 percent in gastric type. The result of this study suggests that type II. Benterocolic type of intestinal rnetaplasia plays a significant role as a precancerous lesion in intestinal type of gastric cancer.

추간판 조직재생을 위한 지방유래 기질세포와 수핵세포의 공배양 조건

최은희 ( Eun Hee Choi ),박광숙 ( Kwang Sook Park ),한인보 ( In Bo Han ),강선웅 ( Sun Woong Kang ),전흥재,이수홍 ( Soo Hong Lee ) 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.4

Nucleus pulposus(NP) cells located at intervertebral disc are chondrocyte-like cells secreting cartilagespecific matrix molecules such as type 2 collagen and aggrecan. NP cells transplantation is used for recovery of damaged intervertebral disc. However, it is difficult to obtain sufficient quantity of NP cells from donor for cell therapy. Recently, adipose derived stromal cells(ASCs) as alternative cell sources have been extensively studied for cells therapy because ASCs can differentiate into several lineages including chondrogenesis. In this study, we sought to optimize the co-culture condition of NP cells and human ASCs for ASC differentiation. Media condition for NP cells and ASCs co-culture was determined by comparing proliferation and differentiation. After that, various cell ratios between NP cells and ASCs(0, 25, 50, 75, 100% ASC) were tested to find optimal cell ratio for efficient differentiation of ASCs by histochemical staining, cell proliferation, gene expression and so on. According RT-PCR and staining, differentiation of ASCs was dependent on the media condition and cell ratio between NP cells and ASCs. This demonstrated the appropriate media and the optimized cell ratio between NP cells and ASCs are able to induce efficient differentiation of ASCs that is expected to provide efficient intervertebral disc regeneration in vivo.