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섬유질계 동시당화발효를 위한 내열성 융합 효모, Kluyveromyces marxianus CHY1612의 개발
강현우(Hyun-Woo Kang),김율(Yule Kim),박주용(Ju-Yong Park),민지호(Jiho Min),최기욱(Gi-Wook Choi) 한국생물공학회 2010 KSBB Journal Vol.25 No.6
To develop thermostable ethanol fermentative yeast strain for lignocellulosic simultaneous saccharification and fermentation, high ethanol producing yeast, Saccharomyces cerevisiae CHY1012 and thermostable yeast, Kluyveromyces marxianus CHY1703 were fused by protoplast fusion. The thermostable fusant, CHY1612 was identified as a Kluyveromyces marxianus by phenotypic and physiological characteristics, as well as molecular analysis based on the D1/D2 domains of the large subunit (26S) rDNA gene and the internally transcribed spacer (ITS) 1 + 2 regions. For lignocellulosic ethanol production, AFEX pretreated barley straw at 150℃ for 90 min was used in a simultaneous saccharification and fermentation (SSF) process using thermotolerant CHY1612. The SSF from 16% pretreated barley straw at 43℃ gave a saccharification ratio of 90.5%, a final ethanol concentration of 38.5 g/L, and a theoretical yield of 91.2%. These results show that K. marxianus CHY1612 has potential for lignocellulosic ethanol production through simultaneous saccharification and fermentation with further development of process.
난백(Egg White)에서 추출한 리소좀 추출물(LOE)의 미백 효능 및 피부장벽에 관한 연구
최다희 ( Da Hee Choi ),전경찬 ( Gyeongchan Jeon ),윤지희 ( Jihee Yoon ),민지호 ( Jiho Min ),박시준 ( Si Jun Park ),김정수 ( Jung Su Kim ),황이택 ( Ee Taek Hwang ),황형서 ( Hyung Seo Hwang ) 대한화장품학회 2019 대한화장품학회지 Vol.45 No.4
리소좀(lysosome)은 진핵세포에서 에너지 대사 및 세포 내 소화 작용에 관여하는 세포 소기관으로 protease, nuclease, glycosidase, lipase, phosphatase 들이 다수 존재한다. 우리는 선행 연구결과들을 통해 난백 리소좀의 멜라닌 색소 탈색능을 보고하였다[8]. 그러나 B16F10 melanocyte 세포주에서 난백 리소좀에 의한 멜라닌 함량 변화 및 피부장벽 조절 연구는 거의 보고되지 않았다. 따라서 우리는 계란 난백(egg white)으로부터 추출한 lysosome-related organelle extract (LOE)에 의한 세포 내 멜라닌 함량 변화 및 피부장벽 강화 효과를 규명하고자 하였다. 먼저 LOE의 미백 효능을 확인하기 위해 B16F10 세포주를 이용하여 세포독성 평가를 진행하였다. B16F10 세포주에서 LOE에 의한 세포독성은 0에서 20 mg/mL 농도에서 관찰되지 않았으나, 40 mg/mL 부터 세포독성이 관찰되어 이후 모든 실험에서 최대 농도값을 20 mg/mL로 설정하였다. 먼저 LOE를 이용한 melanin contents assay 결과, 음성 대조군인 α-MSH 처리군 대비 LOE 처리군 5, 10, 20 mg/mL 농도에서 61.5 ± 4.0%, 61.4 ± 7.3%, 58.3 ± 8.3%로 세포 내 멜라닌 함량이 감소되는 것을 확인하였고, 20 mg/mL 농도 조건에서 MITF 발현 억제도 관찰하였다. LOE의 피부 장벽에 미치는 영향을 관찰하기 위해 각질형성세포주(HaCaT)를 이용하여 TEER (trans-epithelial electrical resistance) assay를 수행한 결과, LOE에 의해 농도 의존적으로 TEER 저항값이 증가하여 LOE가 피부장벽 강화에도 효과가 있음을 알수 있었다. 또한 피부 염증 유발을 위한 TNF-α 처리조건에서도 LOE는 TEER 저항값을 증가시켜 염증 유발 조건에서도 LOE에 의해 피부장벽이 정상적으로 회복되었음을 알 수 있었다. 마지막으로 cell migration assay를 통해 LOE에 의한 세포이동 촉진 효과를 관찰한 결과, LOE는 세포분열 및 세포이동을 촉진시켰다. 위 결과들을 통해 LOE는 미백 기능 뿐 아니라 피부재생 및 피부장벽 강화에도 효과를 나타내는 소재이며, 효소 안정화 및 제형화 기술이 접목된다면 향후 새로운 미백 기능성 화장품 소재로도 개발될 수 있을 것이다. Lysosomes are cellular organelles involved in energy metabolism and intracellular digestion in eukaryotic cells, including protease, nuclease, glycosidase, lipase, and phosphatase. Our previous studies have confirmed that egg white lysosomes had melanin decolorization and reduction activity. However, there have been few studies on skin barrier and skin regeneration as well as inhibition of melanin production by egg white lysosomes on B16F10 melanocyte cell line. In this study, we attempted to identify the effect of lysosome-related organelle extract (LOE) extracted from egg white on the melanin content change and skin barrier enhancement in cells. First, cytotoxicity evaluation was performed on B16F10 melanocyte cell line to confirm the whitening efficacy of LOE. Cytotoxicity by LOE was not observed at 20 mg/mL concentration, but cytotoxicity was observed at 40 mg/mL, and the maximum concentration value was set to 20 mg/mL in all subsequent experiments. LOE samples of 5, 10, 20 mg/mL inhibited melanin production by 61.5 ± 4.0%, 61.4 ± 7.3%, 58.3 ± 8.3%, respectivly, compared to α-MSH, a negative control in melanin contents assay. MITF mRNA expression was reduced by about 39.7 ± 3.2% compared to the α-MSH treatment group. TEER assay using HaCaT showed that LOE increased TEER resistance in a dose-dependent manner, indicating that LOE is involved in strengthening the skin barrier. LOE also increased the TEER resistance under TNF-α treatment. Skin barrier was normally restored by LOE even under the condition of inflammation. LOE had a positive effect on cell division and cell migration promotion, confirmed by the observing the effect of promoting cell migration by LOE through cell migration assay. Taken together, we expect that LOE can be developed as a cosmetic material to enhance has effects on skin regeneration and skin barrier strengthening as well as whitening function if enzyme stabilization and formulation technology are combined.
매몰된 가축 사체의 부패 촉진 및 토양 비옥화를 위한 Corynebacterium glutamicum과 Bacillus licheniformis 처리 효과
신유정 ( You-jeong Shin ),허건영 ( Geon-young Heo ),김주형 ( Ju-hyung Kim ),김빛나 ( Bit-na Kim ),민지호 ( Jiho Min ),조호성 ( Ho-seong Cho ) 한국가축위생학회 2017 韓國家畜衛生學會誌 Vol.40 No.1
Foot and mouth disease (FMD) is highly infectious disease of cloven-hoofed animals, particularly problematic in cattle, sheep, pigs and goats for economic reasons. Last FMD outbreak in February, 2017 caused tremendous social and economical impacts. The Korean FMD policy aims to vaccinate intact animals and euthanize and bury infected animals to prevent the disease spread. However, there was a problem that the buried livestock did not decompose after several years. Therefore, the study was purposed to investigate the effect of Corynebacterium glutamicum and Bacillus licheniformis on the degradation of buried cow carcasses and on the soil condition; such as temperature, decomposition course of carcasses, composition of amino acids in the soil around carcasses, and plant root elongation to measure soil conditions. As a result, the composition of amino acids in the soil treated with C. glutamicum and B. licheniformis was generally higher than those in the untreated soil. Plant roots in soil treated with C. glutamicum and B. licheniformis grew longer than in non-treated soil. The results suggested that the toxic effect on a grave land buried with FMD infected livestock is reduced when treated with C. glutamicum and B. licheniformis in regard of odor reduction, promoted decaying process, and soil fertilization.
LED-식물공장에서 광합성 세균에서 얻은 추출물이 상추의 생육과 품질에 미치는 영향
박주용(Ju-Yong Park),이혜인(Hye-In Lee),김상용(Sang Yong Kim),위지향(Ji-Hyang We),김양훈(Yang-Hoon Kim),민지호(Jiho Min) 한국생물공학회 2020 KSBB Journal Vol.35 No.1
Photosynthetic bacteria, Rhodobacter spharoides, can grow using carbon dioxide as a carbon source and produce physiologically active compounds. This study was carried out to investigate the impact of R. sphaeroides extract on the growth and quality of lettuce(Lactuca sativa L.) in the LED-plant factory. The concentrations of R. sphaeroides extracts(0, 4, 8 and 12 mg/L) were supplemented in medium on one day and 14 days after transplanting, respectively. The growth of lettuce as affected by extract amount was evaluated at 14, 28 and 35 days after transplanting and the contents of minerals and chlorogenic acid were analyzed at 35 days after transplanting. As a result, higher growth was obtained in the treatment with R. sphaeroides extract than the control. The extract of R. sphaeroides showed the best growth at 4 mg/L concentration(leaf width- 120%, leaf length- 109%, number of leaves- 113%, total leaf area- 144%, fresh weight- top: 153%, root: 142%, dry weight- top: 137%, root: 120%) and the highest effect at the early stage of growth on 14 days after transplanting(leaf width- 115%, leaf length- 123%, number of leaves- 124%, total leaf area- 158%, fresh weight- top: 139%, root: 120%, dry weight- top: 130%, root: 139%). In addition, the extract of 4 mg/L concentration improved the content of minerals and chlorogenic acid contained in lettuce. Therefore, R. sphaeroides extract suggested the possibility of producing high-value crops through promotion of growth and quality improvement of lettuce.