麥類와 豆類의 原形質體 融合

Kyung Soo Min(閔庚洙),Tay Eak Whang(黃台益) 한국육종학회 1987 한국육종학회지 Vol.19 No.1

The high frequency of heterokaryons is induced between soybean and barley species by using improved methods of protoplast fusion. Cell wall regeneration and incorporation of¹⁴C - glucose to protoplast in the early stage of culture was observed. The most heteroplasmic fusions occurred in the presence of 30% PEG and 10% dextran among the treated polysaccharides with 7 mM Ca²⁺ and application of centrifugal method than slide glass and micro syringe method. By treatment of 1 ul A₂C and 10 ul DMSO as fusion promoting agents, the protoplast fusion was promoted to 10% and to 80%, respectively. Over 95% of the fused protoplast could be obtained in 15 min. after fusogenics treatment for fusion between protoplasts from different species, but it began to be decreased after 1hr. Cell wall reformation was observed from protoplast and heteroplasmics within 5 days of culture of protoplast after isolation and aggregation from each crops. The maximum binding of ¹⁴C - glucose to cells occurred within 2-3days in individual protoplasts and after 5 days in fused protoplasts in culture.

정규 제왕절개 수술시 예방적 항생제 요법에 관한 연구

민경수 ( Kyung Soo Min ),김홍서 ( Heung Seo Kim ),이민아 ( Min A Lee ),김용일 ( Yong Il Kim ),서영석 ( Young Seok Seo ),이기환 ( Ki Hwan Lee ),이윤이 ( Yun Ee Rhee ) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.9

목적 : 정규 제왕절개 수술시에 예방적인 항생제의 단기 투여가 수술 후 장기, 병합투여와 비교하여 효과가 있는지를 알아보고자 하였다. 연구 방법 : 2001년 1월 1일부터 10월 30일까지의 충남대학병원 산부인과에 입원하여 정규 제왕절개 수술을 시행받은 40명의 여성을 대상으로 수술 전 1회의 예방적인 항생제 투여와 수술 후 24시간 이내에 2회를 투여하였다. 40명의 여성을 대조군으로 수술 후 5회, 또는 6회의 병합요법의 항생제를 투여하였고 이후, 7일 이상의 경구투여를 시행하였다. 수술 후 합병증으로 열성이환, 자궁내막염, 수술부위 감염, 요로감염이 조사되었으며 재원일을 비롯한 다른 비교치를 기록하여 분석하였다. 결과 : 열성이환, 자궁내막염, 수술부위 감염, 요로감염은 두 군간에 유의한 차이를 보이지 않았으며 평균 재원일도 차이를 보이지 않았다. 결론 : 정규 제왕절개 수술에 있어 예방적인 항생제의 단기 투여의 효과는 장기, 병합 투여와의 차이를 보이지 않았다. 예방적 항생제의 단기 투여가 약물의 부작용과 내성을 줄이고 비용 절감에 있어서도 효과를 보일 것으로 생각된다. Objective : To evaluate effectiveness of short-course administration of prophylactic antibiotics in elective cesarean section compared with multiple, long-course administration. Methods : Forty women undergoing elective cesarean section had prophylactic antibiotics administration preoperatively and 2 times more within 24 hours after operation. Forty women was control group who had antibiotics administration postoperatively 5 or 6 times with multiple regimens and 7 days more per oral. Postpartum complications including febrile morbidity, endometritis, wound infection, urinary tract infection were recorded, as were the duration of hospital stay and need for therapeutic antibiotics. Results : Postpartum complications including febrile morbidity, endometritis, wound infection and urinary tract infection had no difference between two groups significantly. Also, their hospital stay had no difference significantly. Conclusion : Prophylactic and short-course antibiotics administration in elective cesarean section is considered to have no difference in comparison with multiple, long-course post-operative administration. Short-course prophylactic antibiotics administration will reduce side effect of drugs and resistance. Also it will provide better cost effectiveness.

목화 陸地棉과 亞細亞棉 種間雜種의 胚珠培養時 生長調節劑와 炭素源의 影響

Kyung Soo Min(閔庚洙),Hong Jae Park(朴洪在) 한국육종학회 1997 한국육종학회지 Vol.29 No.3

This study was performed to increase the efficiency of interspecific hybrid production between Gossypium hirsutum and Gossypium arboreum via in vitro ovule culture. Immature ovules dissected from the interspecific crossed cotton fruit at 4 days after pollination were cultured in Shenck-Hilderbrandt(SH) liquid medium supplemented with various growth regulators and carbon sources. The best results were observed from the combination of 5mM IAA, 0.005mM GA, 0.05mM Kinetin with 18.5% of embryo survival and 10.6% plant regeneration. Glucose and sucrose were considered as reasonable carbon sources for the ovule culture of cotton achieving the highest frequency of plant regeneration at 150mM and/or 200mM, respectively.

Darcy 유동과 Two-equation 모델을 이용한 다공성 방열기 이론해석

민경수(Kyung Soo Min),이대영(Dae-Young Lee),노승탁(Sung Tack Ro) 대한기계학회 2001 대한기계학회 춘추학술대회 Vol.2001 No.3

Theoretical analysis on a porous heat sink is performed in this paper. The flow field inside the porous heat sink due to the impinging flow is obtained using stagnation flow analysis assuming the Darcy flow. Two-equation model is employed to analyze the heat transfer between the impinging air and the porous heat sink considering the temperature difference between the phases. Two important parameters of practical importance are introduced as the unit cell size and the aspect ratio of the porous block, and their influences on the heat transfer performance is investigated. The result shows that there exist optimum values of the aspect ratio and the unit cell size for maximum heat dissipation from the porous heat sink.

유색 보리 종실의 품종별 색소 특성

주완택,민경수,박노동,Ju, Wan-Taek,Min, Kyung-Soo,Park, Ro-Dong 한국응용생명화학회 2003 한국농화학회지 Vol.46 No.4

유색보리 4품종과 보통보리 4품종 종실의 종피에 함유되어 있는 색소의 특성을 분석하여 해당작물의 신품종 육성시 우수계통 선발에 유용한 자료를 제공코자 하였다. 유색(검정)쌀보리인 Ab2231을 표준시료로 하여 실험한 결과 에탄올의 농도가 80%일 때 색소추출 효율이 양호하였다. 80% 에탄올에 1.0% 염산을 첨가한 것이 추출효율이 가장 좋았다. 추출시간에 따른 추출효율성을 조사한 결과 1시간 추출이면 충분하였다. 추출된 색소의 안정성을 유지하기 위하여 첨가시키는 산의 적정농도를 알아본 결과 1.0% HCI을 첨가한 농도에서 가장 낮은 색소량 감소율이 나타났다. 각 보리 품종 시료에서 추출한 색소의 흡광스펙트럼을 조사하였던 바, Ab2231의 그것은 535 nm에서 최대흡광파장을 나타내어 안토시아닌계 색소로 판단되었으며, 호계4호의 그것은 안토시아닌계 이외의 색소를 주로 함유하는 것으로 보였다. 한편, 추출된 색소의 특성에 따라 유색보리의 품종은 Ab2231와 모찌무기로 대표되는 쌀보리 그룹과, Lion과 호계4호로 대표되는 겉보리 그룹으로 분류되었다. The characteristics of the pigments extracted from grains of the colored barley varieties Ab2231, Mozzimugi, Lion, and Hogye 4 were studied. Extraction of the pigments from powder (80 mesh) of the barley grains was most efficient in 80% ethanol containing 1.0% HCI for 1 hr at $60^{\circ}C$. The pigments were also stable in 80% ethanol containing 1.0% HCI. Depending on the absorption spectra, maximal peak wavelength, and the ratio of $A_{440}/A_{max}$, the varieties were separated into two groups, colored naked barley (Ab2231 and Mozzimugi) and colored covered barley (Lion and Hogye 4).

체외순환과 초저체온 순환정지술을 이용한 내경동맥의 거대 뇌동맥류 수술 - 증 례 보 고 -

정유남,민경수,이무섭,김동호,홍종면,김상태,Chung, You Nam,Min, Kyung Soo,Lee, Mou Seop,Kim, Dong Ho,Hong, Jong Myeon,Kim, Sang Tai 대한신경외과학회 2000 Journal of Korean neurosurgical society Vol.29 No.12

The authors report a case of a 72-year-old woman who presented with intraventricular and subarachnoid hemorrhage of Hunt and Hess grade IV after the rupture of a giant aneurysm arising from the right internal carotid artery. The aneurysm was clipped successfully with the aid of cardiopulmonary bypass(closed chest method), deep hypothermic circulatory arrest, and cerebral protection with barbiturate resulting in moderate disability. We discuss the usefulness and problems related to technique of circulatory arrest and cardiopulmonary bypass using closed chest method, and suggest the possible benefits of open chest method in elderly people and the importance of preoperative plan to coordinate anesthesia and operation.

인체 암세포주에서 방사선감수성의 지표간의 상호관계

박우윤,김원동,민경수,Park, Woo-Yoon,Kim, Won-Dong,Min, Kyung-Soo 대한방사선종양학회 1998 Radiation Oncology Journal Vol.16 No.2

목적 : 임상에서 발생빈도가 높은 위선암, 폐선암, 망막세포종, 자궁경부 편평상피암의 4가지 인 체암세포주(MKN-45, PC-14, Y-79, HeL)를 이용하여 방사선조사후 세포생존분획 및 세포생존곡선의 모양을 결정하는 지표를 구하고 방사선조사후 손상 회복정도를 측정하여 이들 여러 지표간의 상관관계 여부를 구명하기 위하여 본실험을 시행하였다. 대상 및 방법 :각 세포주의 방사선감수성을 보기 위하여 1, 2, 3, 5, 7 및 10Gy의 방사선을 1 회 조사하였고 방사선조사후 준치사손상 회복정도를 보기 위하여 5Gy씩 2회의 방사선조사를 0, 1, 2, 3, 4, 6 및 24시간 간격으로 시행하였다. 세포의 생존분획은 $Sperman-K\"{a}rbor$ 방법으로 세포집락형성능력을 측정하여 산출하였으며 생존곡선의 수학적 분석은 linear-quadratic(LQ), multitarget-single hit(MS) 모형과 mean inactivation $dose(\v{D})$를 이용하였다. 결과 : 방사선조사후의 세포생존 실험결과 2Gy에서의 세포생존분획(SF2)은 0.174에서 0.85까지 다양하게 나타났으며 Y-79는 유의하게 낮은 SF2를, PC-14는 높은 SF2를 나타내었다(p<0.05, t-test). LQ model로 분석한 방사선 세포생존곡선의 분석결과 Y-79, MKN-45, HeLa, PC-14에서 ${\alpha}$가 각각 0.603, 0.355, 0.275, 0.102이었고 ${\beta}$는 각각 0.005, 0.016, 0.025, 0.027이었다. MS model로 분석한 결과는 Y-79, MKN-45, HeLa, PC-14에서 Do가 각각 1.59, 1.84, 1.88, 2.52였고 n은 0.97, 1.46, 1.52, 1.69를 보였다. 한편 Gauss-Laguerre방법으로 계산한 $\v{D}$는 Y-79, MKN-45, HeLa, PC-14에서 각각 1.62, 2.37, 2.61, 3.95였다. SF2가 감소함에 따라 ${\alpha}$값은 증가하였고 Do, $\v{D}$값은 감소하였으며 이들간의 Pearson 상관계수는 각각 0.953, 0.993, 0.999였다. (p<0.05). 분할조사에 의한 준치사손상 회복정도는 약 4시간 내외에 포화상태에 도달하였으며 포화상태의 recovery ratio(RR)는 2에서 3.79 사이였다. RR은 방사선감수성의 지표인 SF2, ${\alpha}$, ${\beta}$, Do, $\v{D}$과 통계학적으로 유의한 상관관계를 나타내지 않았다. 결론 : 본 연구의 결과 네가지 인체상피암세포주의 내재적 발사선감수성은 서로 상이하였으며 Y-79가 가장 민감하였고, MKN-45와 HeLa는 각각 중등도의 방사선감수성을 나타냈으며 PC-14는 방사선감수성이 가장 낮았다. 이와같은 감수성의 차이는 SF2, ${\alpha}$, Do와 $\v{D}$의 차이로 나타났으며 띠들간에는 밀접한 상관관계를 나타내었다. 방사선에 의한 준치사손상 회복력은 MKN-45와 HeLa에서 높게 나타났고 회복력과 방사선감수성과는 무관하였다. 각 암세포주에 따르는 이와같은 지표들은 향후 방사선치료 효과를 높이기 위한 방사선생물학 실험의 기초 자료로서 이용되어 질 수 있을 것이다. Purpose : We conducted clonogenic assay using human cancer cell lines (MKN-45, PC-14, Y-79, HeLa) to investigate a correlation between the parameters of radiosensitivity. Materials and Methods : Human cancer cell lines were irradiated with single doses of 1, 2, 3, 5, 7 and 10Gy for the study of radiosensitivity and subrethal damage repair capacity was assessed with two fractions of 5Gy separated with a time interval of 0, 1, 2, 3, 4, 6 and 24 hours. Surviving fraction was assessed with clonogenic assay using $Sperman-H\"{a}rbor$ method and mathematical analysis of survival curves was done with linear-quadratic (LQ) , multitarget-single hit(MS) model and mean inactivation dose$(\v{D})$. Results : Surviving fractions at 2Gy(SF2) were variable among the cell lines, ranged from 0.174 to 0.85 The SF2 of Y-79 was lowest and that of PC-14 was highest(p<0.05, t-test). LQ model analysis showed that the values of $\alpha$ for Y-79, MKN-45, HeLa and PC-14 were 0.603, 0.356, 0.275 and 0.102 respectively, and those of $\beta$ were 0.005, 0.016, 0.025 and 0.027 respectively. Fitting to MS model showed that the values of Do for Y-79. MKN-45, HeLa and PC-14 were 1.59. 1.84. 1.88 and 2.52 respectively, and those of n were 0.97, 1.46, 1.52 and 1 69 respectively. The $\v{D}s$ calculated by Gauss-Laguerre method were 1.62, 2.37, 2,01 and 3.95 respectively So the SF2 was significantly correlated with $\alpha$, Do and $\v{D}$. Their Pearson correlation coefficiencics were -0.953 and 0,993. 0.999 respectively(p<0.05). Sublethal damage repair was saturated around 4 hours and recovery ratios (RR) at plateau phase ranged from 2 to 3.79. But RR was not correlated with SF2, ${\alpha}$, ${\beta}$, Do, $\v{D}$. Conclusion : The intrinsic radiosensitivity was very different among the tested human cell lines. Y-79 was the most sensitive and PC-l4 was the least sensitive. SF2 was well correlated with ${\alpha}$, Do, and $\v{D}$. RR was high for MKN-45 and HeLa but had nothing to do with radiosensitivity parameters. These basic parameters can be used as baseline data for various in vitro radiobiological experiments.

보리再培種과 野生種(hordeum bulbosum) 間 雜種 幼胚의 發育增進

Dong Hee Chung(鄭東熙),Kyung Soo Min(閔庚洙),Jong Un Chun(千鍾殷) 한국육종학회 1994 한국육종학회지 Vol.26 No.1

The purpose of this study is to investigate effects of materials and colors of crossing bags, removal of florets in a spike on formation and development of seed set and embryos, and to observe development of haploidy and diploidy embryos at different days after pollination for bulbosum method. Cellophane, polyethylene or paper was not suitable for crossing material, because the florets bagged with those materials turned to yellow and died not long after pollination. The humidity within the bags dropped too low when cellophane or paper was used, due to their porosity. While it rised too high upto dew point when polyethylene was used for its air-tightness. Double bags of parchment(16㎝ long)+ polyethylene (10㎝ long) which kept the temperature inside bags around 25℃, gave the best rates of formation and development of the embryos. The parchment inside functioned as a buffer against moisture by absorbing and releasing water properly. Path analysis with temperature, moistutre and light intensity inside the bags indicated moisture was the primary factor mostly influencing the embryo development. Removal of rows in a spike as a means of thinning out florets was not effective to improve development of embryo substantially. From the comparative observation on the haploid development of the seed set by H. bulbosum pollinator with diploid those set by selfing, it was noticed that the former had much smaller embryos, lacking morphologically differentiated organs in 14 days after pollination. The endosperm which had few starch particles in 11 days, was completely liquidified in 14 days after pollination.

Hordeum bulbosum 을 利用한 半數性 보리의 同定

Dong Hee Chung(鄭東熙),Kyung Soo Min(閔庚洙),Jong Un Chun(千鍾殷) 한국육종학회 1994 한국육종학회지 Vol.26 No.3

This study was conducted to authenticate barley haploids by cytological, electrophoretical and morphological observations in order to establish an effective bulbosum method. Somatic chromosome numbers were 7 in the suspected barley hybrids compared to 14 in both H. bulbosum and H. vulgare, even though the chromosomes of the two species were not exactly distinguished. Barley haploid was clearly shown because of the different isozyme patterns of the two species, and suspected barley haploids. The colchicine-induced diploids with H. bulbosum chromosomes always displayed several characteristics of H. bulbosum, especially prostrate habit of growth. Progeny of the diploids was morphologically identical to their seed parents, which was the additional proof that they were diploids of genetically complete barley haploids. Genotypic variation for haploid embryo formation in eleven selected Korean barley cultivars ranged from 15 to 52%, and the variation for plant development from embryo culture ranged from 18 to 55%. H. bulbosum pollinator, GBC gave a little better results for embryo formation and plant development than Sp. D. did.

버어리種 담배(nicotiana tabacum L.)의 主要 量的形質에 對한 遺傳分析 2. F₁, F₂世代의 組合能力

Chun Joon Jo(趙天俊),Kyung Soo Min(閔庚洙) 한국육종학회 1987 한국육종학회지 Vol.19 No.2

Ten burley varieties of Nicotiana tabacum L., 45 F₁ hybrids and 45 F₂ populations among them were evaluated at Jeonju Experiment Station, Korea Ginseng & Tobacco Research Institute in 1985. Estimated variance for general combining ability (GCA) was highly significant for all eleven characters studied. Specific combining ability (SCA) variance was also significant for all characters except leaf length in F₂ generation, but conspicuously smaller than that of GCA. Highly significant positive GCA effects were observed for yield in White Burley, TI 1068. But in Aurelius and L8, negative GCA effects were observed for most of characters. The correlations of parental GCA effects and their ranks between F₁ and F₂ generations were significantly positive. White Burley x L8, Ky 17 x L8, Va 509 x L8 and Ky 12 x L8 expressed relatively high SCA effects for yield. And Burley 21 x Va 528, Ky 12 x TI 1068 and Va 528 x Ky 17 showed high SCA effects in the direction of decreasing the total alkaloid content.