국내 옥수수 순계주에서 CP4 5-Enol- Pyruvylshikimate-3- Phosphate Synthase 유전자의 발현

조미애,권석윤,김진석,이병규,문추연,최필선,Cho, Mi-Ae,Kwon, Suk-Yoon,Kim, Jin-Seog,Lee, Byoung-Kyu,Moon, Choo-Yeun,Choi, Pil-Son 한국식물생명공학회 2007 식물생명공학회지 Vol.34 No.4

국내 옥수수 순계주에서 Agrobacterium 공동배양으로 CP4 5-Enol-pyruvylshikimate-3-phosphate synthase (CP4 EPSPS) 유전자가 도입된 제초제저항성식물체를 개발하였다. 5개의 순계주 (HW1, KL103, HW3, HW4, HW7)의 미숙배를 Ubiquitin promoter-CP4 EPSPS 유전자와 CaMV35S promoter-nptII 유전자가 발현되도록 제조된 pCAMBIA2300 벡터를 C58C1 Agrobacterium에 형질전환하여 공동 배양하였다. 항생제로 paromomycin이 첨가된 배지에서 선발된 옥수수 형질전환체를 PCR, RT-PCR 및 Northern 분석을 통하여 유전자의 도입과 발현을 확인하였다. 또한 형질전환 식물체의 glyphosate 처리에 따른 shikimate 축적반응을 확인하였다. Paromomycin 저항성 캘러스 형성빈도는 옥수수 각 순계주 HW1, KL103, HW3, HW4, HW7에서 각각 0.37%, 0.03%, 2.20%, 2.37%, 0.81%로 나타났으며, PCR분석을 통하여 최종적으로 2개의 옥수수 순계주 (HW3, HW4)의 paromomycin 저항성 캘러스로부터 분화된 식물체에서 확인하였다. 이러한 형질전환체중에서 RT-PCR 및 Nothern blot 분석을 통하여 CP4 EPSPS 유전자가 발현되는 2개의 계통 (M266, M104) 을 선발하였고, shikimate 축적반응을 통하여 glyphosate에 대한 저항성을 갖는 계통 (M266)을 최종적으로 선발하였다. 이러한 결과는 국내 옥수수 순계주에서 제초제저항성을 갖는 옥수수 형질 전환체를 개발할 수 있음을 시사한다. This study was conducted to develop herbicide-resistance domestic maize plants by introducing the CP4 5-enol-pyruvylshikimate-3-phosphate synthase (CP4 EPSPS) gene using Agrobacterium tumefaciens-mediated immature embryo transformation. Immature embryos of five genotypes (HW1, KL103, HW3, HW4, HW7) were co-cultivated with strains Agrobacterium tumefaciens (strain C58C1) containing the binary vector (pCAMBIA2300) carrying Ubiquitin promoter-CP4 EPSPS gene and Cauliflower mosaic virus 35S (CaMV35S) promoter-nptll gene conferring resistance to paromomycin as a selective agent. The presence and expression of CP4 EPSPS transgene were confirmed by PCR, RT-PCR and Northern blot analysis, respectively. Also, the resistance to glyphosate in the transgenic maize ($T_1$) was analyzed by shikimate accumulation assay. The frequency (%) of paromomycin-resistance callus was 0.37, 0.03, 2.20, 2.37, and 0.81% in pure lines HW1, KL103, HW3, HW4 and HW7, respectively. EPSP transgene sequences were amplified in putative transgenic plants that regenerated from paromomycin-resistance calli of two inbred lines (HW3, HW4). Of them, RT-PCR and Northern blot analyses revealed that the transgene was only expressed in two transgenic events (M266, M104) of HW4 inbred line, and a mild glyphosate resistance of transgenic event (M266) was confirmed by the lower shikimate accumulation in leaf segments. These results demonstrate that transgenic maize with herbicide-resistance traits in Korean genotype can be genetically obtained.

질소 함유 메소포러스 할로우 카본에 의한 페놀 폐수의 처리

류하오 ( Hao Liu ),장성호 ( Seong-ho Jang ),문추연 ( Choo-yeun Moon ),이재용 ( Jae-yong Lee ),박진식 ( Jin-sick Park ),정병길 ( Byung-gil Jung ) 한국폐기물자원순환학회(구 한국폐기물학회) 2019 한국폐기물자원순환학회 추계학술발표논문집 Vol.2019 No.-

In recent years, many kinds of literature on the nanotechnology used in medicine, electrochemistry, wastewater treatment and other fields have been reported. It can be highly efficient, economical, multifunctional and reduce the dependence on large treatment equipment when wastewater treatment is processed using nanotechnology. The property of carbon materials designed by nanotechnology can be more suitable for the demand of adsorbent than activated carbon on wastewater treatment. And the different types of carbon nanomaterials have been reported to show different properties and unique adsorption mechanisms. Although the carbon nanomaterials have various advantages in adsorbent, they also have drawbacks that carbon is hydrophobic at the pristine state due to their surface without acidic groups or basic groups. In order to improve the hydrophilicity of the carbon materials, plasma can be Introduced onto the surfaces of carbon and the oxygen plasma developed acidic groups and nitrogen plasma and ammonia plasma developed basic groups. In the other side, the adsorption capacity on phenol can enhance by introducing pyridinic and pyrrolic groups as the basic groups. In this work, The adsorbent - nitrogen doped magnetic mesoporous hollows carbon (N-MMHC) were synthesized through one-pot coating strategy using tetrapropyl-orthosilicate (TPOS), resorcinol, formaldehyde and Fe₂O₃ nanoparticle. Ethylenediamine( EDA) as both catalyst and nitrogen sources in the process.

QUAL2E에 의한 兄山江 水質豫測

이태호(Tea-Hoo Lee),김인환(In-Han Kim),문추연(Choo-Yeun Moon) 한국환경관리학회 1996 環境管理學會誌 Vol.2 No.1

상수원수 수질변화에 따른 전오존 처리효과 및 경제성 평가

최동훈 ( Dong Hoon Choi ),박진식 ( Jin Sik Park ),문추연 ( Choo Yeun Moon ),이재용 ( Jae Yong Lee ),유동춘 ( Dong Choon Ryu ),장성호 ( Seong Ho Jang ),권기원 ( Ki Won Kwon ),이수애 ( Soo Ae Lee ) 한국환경과학회 2013 한국환경과학회지 Vol.22 No.4

This study, changes in raw water quality is to indicate on the efficiency of ozone treatment of each pollutant as compared to derive the appropriate operating measures. The appropriate selection for injection rate of pre-ozone and did not inject pre-ozone assess changes in the water. When good water quality, you not injected of pre-ozone to evaluate the economic efficiency of electricity and put the most cost-effective ozone concentration were evaluated. Evaluation remove organic matter and chlorophyll-a concentration level in experiments with each factor of the water DOC> 2.5㎎ / L, THMFP> 70㎍ / L, Chl-a> 30㎎/㎥or less constant process, if you do not need to put pre-ozone showed little impact. It also does not put you in pre-ozone appropriate produce enough power rate savings was calculated as approximately 90 million won. Ability to remove organic materials and the ability to produce disinfection byproducts, and cost-effective decisions by considering the concentration of injection if pre-ozone 1 mg/L was investigated by the appropriate concentration of ozone injection.

전기화학적 처리에서 pH 및 전해질이 제거효율에 미치는 영향

이재용 ( Jae Yong Lee ),장성호 ( Seong Ho Jang ),홍성철 ( Sung Chul Hong ),이병인 ( Pyong In Yi ),김한수 ( Han Soo Kim ),박진식 ( Jin Sik Park ),문추연 ( Choo Yeun Moon ) 한국수처리학회 2014 한국수처리학회지 Vol.22 No.5

Conventional wastewater treatment processes are inefficient for the removal of these types of toxic and hazardous pollutants from wastewater. Different cathodes as working electrode and anodes as counter electrode used in this process are analyzed. Also various pollutants removed by this process are evaluated. Quick removal and mineralization of pollutants and their intermediate reaction products were reported. The effects of initial pH, electrolyte on the performance of electrochemical technique are investigated by using sacrificial SUS 316 electrodes. CODCr, Oil & Grease and T-N removal characteristics of metalworking fluid by electrochemical process at initial pH 4, 7, 10 showed that CODCr removal efficiency were 66.9%, 58.3% and 24.2%, respectively. CODCr removal efficiency of metalworking fluid depended on the change of electrolytes showed that the removal efficiency of 42% to 47% in Na2SO4, H2SO4 and HNO3. However, NaCl showed high removal efficiency of 71%, approximately 30% higher than that of other electrolytes.

VIP 공정을 이용한 생활하수의 처리에 관한 연구

문추연,김동일,김수생 동아대학교 생산기술연구소 2000 生産技術硏究所硏究論文集 Vol.5 No.2

Recently in Korea and developed countries, nitrogen and phophorus removal are the main target to improve effluent qualities, in order to protect discharge basins from eutrofication by N and P. Introduction of an advanced treatment function to existing wastewater treatment systems, which can be removed nitrogen and phophorus as well as BOD, COD and SS, have been required to protect water resources from domestic and industrial wastewaters. For the improvement of effluent quality through nitrogen(N) and phophorus (P) removal, this study conducted to investigate and analyse of the municlpal sewage treatment process such as VIP(Virginia Initiative Plant) process on cost effectiveness operating and improvement of effluent quality. Based on the results of the experiments, The average removal efficiencies of organic matter were BOD 87.4 ~ 91.8%, COD 77.7 ~ 81.3%. At influent concentration of T_-N 41.8mg/l, NH_4-N 36.2 mg/l, T_-P 3.14mg/l and PO_4-P 1.6 mg/l, nutrient removal efficiencies were NH_4-N 95.9%, T_-N 67.8%, PO_4-P 69.9%, and T_-P 70.7%.

AB-PROCESS에 의한 燐除去에 관한 硏究

문추연,안종수,김수생 동아대학교 환경문제연구소 1993 硏究報告 Vol.16 No.1

The Purpose of this research is aimed to find out optimum operating conditions for Phosphorus removal by AB-Process. The Comparision between a partially aerated at B-step and fully aerated AB-Proecess was made at different mixing and aeration time, detention time. The BOD removal efficiency showed up to 90% in the partially aerated at B-step as well as fully aerated process. In the partially aerated process at B-step the substrate removal efficiency remained constant irrespective of anaerobic/aerobic reactor retention time ratios. Phosphorus removal efficiency in the partially aerated process at B-step was higher than that in the fully aerated AB-Process. Phosphorus removal efficiency in the process tended to increase as the anaerobic reactor volume in the B-step increased.

도시생활하수 특성 분석에 관한 연구

문추연,이동섭 경운대학교 산업기술연구소 2002 産業技術硏究論文誌 Vol.5 No.1(A)

The purpose of this study is characteristic analysis of municipal sewage with source(union, business, combination and individuality). In the analysis results of union sample, average concentration in business area of TBOD, TCOD_Mn and TCOD_Cr were 190㎎/l, 83㎎/l and 336㎎/lℓ, respectively. And TCOD_Cr /TBOD ratio was 1.8. TBOD of union sample from individuality house area was 99㎎/l. In the analysis results of business area sample, average concentration of TBOD, TCOD_Mn and TCOD_Cr were 127㎎/l, 83㎎/l and 243㎎/l, respectively. Average concentration of T-N was 51.8㎎/l. In the analysis results of combination house area sample, average concentration of TBOD was 128 and TCOD_Cr /TBOD ratio was 1.7. In the analysis results of individuality house area sample, average concentration of TBOD, TCOD_Mn and TCOD_Cr were 87㎎/l, 46㎎/l and 218㎎/l, respectively, and TCOD_Cr /TBOD ratio was 2.5.

兄山강 流域의 汚染負荷量豫測에 관한 연구

文秋淵,朴鎭植,金秀生 동아대학교 환경문제연구소 1995 硏究報告 Vol.18 No.2

This study has performed to investigate and evaluate the amount of pollutants and the status of water quality of the Hyung-San River and prediction water pollution loads. In the field survey('94.11-'95.3), mean concentrations of BOD were found 0.78-4.90ppm, respectively, which were exceeding the second grade at inflow point of Sindang stream The predicted BOD pollution loads of Hyung-San river in 1996, 2001, 2006, 2011 were 13,068.3 kg/day, 13,003.1 kg/day, 9,355.6 kg/day, 9,506.6 kg/day.

도시재개발지구 선정기준과 사업의 개선방안에 관한 연구

이상관,문추연,김수생 동아대학교 건설기술연구소 1999 硏究報告 Vol.23 No.2

This study aims to examine current regulations regarding to urban renewal and slum clearance within the urban areas and to propose implemental strategy to enhance the efficiency the urban renewal projects. To accomplish these objectives this study reviews three different redevelopment projects, such as dwelling environment enhancement project, inner city redevelopment project, and slum clearance project, completed within the Taegu Metropolitan city. Findings of this study show that in order to provide rational criteria for the various urban renewal projects selected criteria which can determine the conditions of surrounding environment should be classified as detail as possible and also these criteria have to be able to considered in a numerical format. Finally, this study emphasizes the importance of citizen participation in the urban renewal projects to achieve the projects' original goals and public concerns.