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김위식 ( Wi Sik Kim ),장민석 ( Min Seok Jang ),정성주 ( Sung Ju Jung ),김석렬 ( Seok Ryel Kim ),박명애 ( Myoung Ae Park ),이정호 ( Jeong Ho Lee ),명정인 ( Jeong In Myeong ),오명주 ( Myung Joo Oh ) 한국어병학회 2011 한국어병학회지 Vol.24 No.1
The specific antibody response of olive flounder, Paralichthys olivaceus to different water temperature were tested by enzyme-linked immunosorbent assay (ELISA). In the rearing temperature of 15℃, first anti-bovine serum albumin (BSA) antibody titer was appeared after 14 days of immunization, whereas 24∼48 days post-immunization (PI) resulted maximum antibody titer in all 5 experimental fish with optical density (OD) values 1.94∼3.04. At the end of the experiment (84 days), 0.03∼1.28 OD values were observed. In the rearing temperature of 12∼13℃, first antibody titer was found 28 days PI in 2 out of 5 fish. Three fish shown high OD titer (1.88∼2.68) between 56 and 70 days and OD values of 0.49 to 2.35 were observed at 84 days. However, the anti-BSA antibodies of two fish showed less than 0.8 OD values until 84 days. In the rearing temperature of 10℃, specific antibody appeared at 56 days, maximum antibody titer was observed at 70 days in 2 out of 5 fish (OD values: 1.37∼1.53) and 1.00 to 1.11 OD values were observed at 84 days. Rest 3 fish showed OD values of 0.12 to 0.68 much below to that of other 2 fish, throughout the experimental period. In conclusion, specific antibody response of olive flounder at high temperature was much faster, higher and longer than that at lower temperature.
외인성 갑상선호르몬 ( T₃ ) 의 경구투여가 조피블락 ( Sebastes schlegeli ) 치어의 생리적 상태 , 성장 및 생존에 미치는 영향
강덕영(Duck Young Kang),장영진(Young Jin Chang),김윤(Yoon Kim),명정인(Jeong In Myoung) 한국수산과학회 2001 한국수산과학회지 Vol.34 No.6
조피볼락, S. schlegeli 치어의 생리, 성장 및 생존율에 미치는 3, 5.3`-triiodo-L-thyronine (T₃) 효과를 파악하기 위해 T₃를 0, 5, 10 및 15 ppm으로 사료에 섞어 경구투여하였다. 실험 종료시 섭식률 및 사료효율은 10 PPM구가 가장 높게 나타났으며, TCH는 처리농도가 높아질수록 낮은 TCH값을 나타내었다. 실험구별 기형률은 T₃처리 농도가 높아질수록 높게 나타났다. 실험 종료시 체성분 분석 결과, 수분과 회분 함량은 실험구간 유의차를 찾아 볼 수 없었고, 단백질 함량은 15PPM구가 5 PPM구를 제외한 모든 실험구에 비해 많았으며, 지질 함량의 경우 15 PPM구가 대조구에 비해 적었고, 5 PPM구와 10 PPM구는 대조구와 큰 차이가 없었다. 실험구별 길이 및 무게 성장은 10 PPM구가 지속적인 성장을 보여 실험 종료시 9.80±0.26㎝로 대조구에 비해 빠른 성장을 보였다. 실험구별 비만도는 T₃구가 18.9∼19.0으로 대조구와 유의한 차이를 나타내지 않았다. 각 실험구별 사육어의 생존율은 CONTROL구가 가장 높았고, CONTROL구를 재외하고는 10 PPM구가 다른 실험구에 비하여 높게 나타났다. Rockfish (Sebastes schlegeli) juveniles were fed with the diets containing 0 (control and sham), 5, 10 and 15 ppm of 3,5,3`-triiodo-L-thyronine (T₃) for 50 days to assess the effect of the hormone on the change of physiological condition, growth and survival rate. Fish were fed the commercial diet by hand to satiation 2 times per day. After 50 days, food intake, feed efficiency, thyroid cell height (TCH), abnormality, proximate body composition, growth, condition factor and survival rate were also examined. The food intake and the feed efficiency of S. schlegeli fed with diet containing 10 ppm of T₃ was significantly higher than those of fishes fed with the other diets. On the final day of experiment, atrophy of thyroid gland was observed in fish administered with 10 and 15 ppm of T₃. T₃ increased slightly the abnormality according to the increase of T₃ dose. The whole body proximate analyses indicated that the fishes administrated with 15 ppm of T₃ were the highest in protein content and were the lowest in lipid, but in ash content were there a significant effects of T₃. The growth of S. schlegeli fed with a diet containing 10 ppm of T₃ was significantly higher than that of control. The condition factor was not related to administered T₃ content. T₃ slightly improved the survival rate of juvenile S. schlegeli, and the survival rate of fish administered with 10 ppm was significantly higher than that of sham-control but was lower than that of control.