생쥐에서 종양세포의 암유전자발현에 대한 울솔산의 효과

류태형(Tae Hyong Rhew),박성미(Sung Mi Park),박건영(Kun Young Park),정해영(Hae Young Chung),하재청(Jae Chung Hah),이정규(Chung Kyu Lee) 대한약학회 1992 약학회지 Vol.36 No.6

To investigate the effect of ursolic acid on the expression of oncogenes in tumor cells of mice, sarcoma 180 ascites tumor cells were implanted into the left groin of ICR mice and the tumor bearing mice were treated with ursolic acid. The expression of oncogenes were measured by in situ hybridization method. Ursolic acid significantly reduced the expression of oncogenes in the tumor cells. Therefore, it can be said that the prestated anticarcinogenic effect of ursolic acid could be partly ascribed to the mechanism included in the oncogene''s transcription level.

식품, 영양과 암의 관계

류태형(Tae-Hyong Rhew) 한국식품영양과학회 1985 한국식품영양과학회지 Vol.14 No.3

There is a trend that the total number of cancer cases is steadily increasing as the population grows. It has been estimated that 85% of the cancer rate in the U. S. is attributed to environmental factors. Among the enviromental factors, diet and nutrition appear to be related to the largest number of human cancers.<br/> Diet and nutrition might be related to cancer by several mechanisms. Food may contain a direct carcinogen or precursors that become carcinogens by spontanous reactions, or by host metabolism, or through the actions of microbial flora. Chemicals that cause cancers generally have reactive electrophilic centers which can combine with electron-rich atoms in nucleic acids and cause cancers by changing the genetic activity of the cells. A variety of factors in foods might be involved in the etiology of carcinogenesis.<br/> Chemicals in food that cause cancers include carcinogens of plants and animal origin and also those in drinking water. Other then these, fungal metabolites alcohol, asbestos, heavy metals, pesticides, and food additives might be included as food carcinogenesis. The method of cooking foods also might contribute to carcinogenesis. Some chemicals in foods act as promoters in carcinogenesis.<br/> Prevention of cancers by dietary practises have received much interest. Consumption of certain vegetables or cellulose can reduce carcinogenic activity of several compounds. A variety of antioxidants or micronutrients may be effective anticarciongens.<br/> Glutathione in the soluble fraction of the cells, is a major defense against oxidative and alkylating carcinogens. Recently anticarcinogenic activity of chlorophyll was demonstrated. Daily consumption of milk appears to effectively reduce stomach cancer.

Effect of Selected Persimmon Leaf Components against Sarcoma 180 Induced Tumor in Mice

김병기(Byeong-Gee Kim),류태형(Tae-Hyong Rhew),최은상(Eun-Sang Choe),정해영(Hae-Young Chung),박건영(Kun-Young Park),이숙희(Sook-Hee Rhee) 한국식품영양과학회 1993 한국식품영양과학회지 Vol.22 No.3

Sarcoma 180 복수형 종양세포를 생쥐의 왼쪽 서혜부에 이식한 후 감잎에서 분리한 tannin, chloroform fraction 추출물 및 2, 4-decadienal을 복강 내에 각각 단독투여하여 그 효과를 형태학적으로 검토하였다. 이식된 sarcoma 180 육종에서 암화의 진행을 지연시키는데는 tannin 투여시에 가장 효과적이었고 chloroform fraction 추출물 및 2, 4-decadienal 투여시에는 형태적으로 간주할 수 있는 지연효과는 없는 것으로 나타났다. 감잎 tannin은 종양세포를 선택적으로 파괴하여 종양의 증식을 억제하는 것이 아니라 일종의 혐기상태의 세포 환경에서 종양을 괴사과정으로 유도하지않고, 발암성을 가지는 단백질과 같은 물질의 합성을 저해하거나 또는 자식작용을 가진 과립들로 하여금 이들을 소화시켜 이식된 종양세포를 악성종양으로 유도하는 과정을 지연시키는 효과를 나타내었다. Antitumor activities of tannin extract and chloroform fraction extract from the persimmon leaves, and 2, 4-decadienal identified as an antimutagenic compound in persimmon leaves were examined in sarcoma 180 implanted tumor in mice by using both light and transmission electron microscopes. Among them, tannin extracted from the persimmon leaves delayed the progression of malignant tumor but the other two did not show any noticeable effect. The antitumorigenic activity of tannin extract might not come from the selective cytotoxicity against tumor cells, but might be, in an anaerobic environment, from the inhibitory action against oncogenic protein synthesis or from the proteolysis of the pre-formed oncogenic proteins by autophagocytic granules. Therefore, the tannin from persimmon leaves might protect cells from fast progression of malignant tumorigenesis.

Effects of Ursolic Acid Isolated from Eriobotrya japonica on c - myc and c - Ha - ras Oncogene Expression at Sarcoma 180 Cell

최양애(Yang-Ae Choi),류태형(Tae Hyong Rhew),박건영(Kun-Young Park),정해영(Hae-Young Chung),하재청(Jae-Chung Hah) 한국식품영양과학회 1992 한국식품영양과학회지 Vol.21 No.3

비파엽에서 분리동정한 활성성분인 울솔레산을 sarcoma 180 cells에 처리하여 c-myc 과 c-Ha-ras 암유전자 발현에 있어서 변화를 조사하였다. 그 결과 c-myc유전자의 발현에서는 뚜렸한 감소가 관찰되었으나 c-Ha-ras 유전자 발현은 대조군과 거의 차이가 없었다. Cell proliferation에 중요한 역할을 하는 것으로 추측되고 있는 c-myc 유전자 발현의 감소는 지금까지 보고된 그 물질의 an-tipromotional effect와 관계있는 것으로 보여진다. The sarcoma 180 cells were treated with ursolic acid which was previously extracted from leaves of Eriobotrya japonica Lindy (Rosaceae) and identified as a potent anticarcinogenic agent. Suppressing effects of the compound with testing changes in selected oncogenes expression were examined by using the northern hybridization method. Ursolic acid significantly suppressed c-myc oncogene expression. However, c-Ha-ras oncogene expression was lowered slightly with the ursolic acid treatment. Therefore, it was concluded that preproven anticarcinogenic effects of ursolic acid should be partly ascribed to the modified oncogenic expression.

들미나리추출물이 마우스의 세포성면역에 미치는 효과

김광혁(Kwang Hyuk Kim),장명웅(Myung Woong Chang),박건영(Kun-Young Park),이숙희(Sook-Hee Rhee),류태형(Tae Hyong Rhew),선우양일(Yangil Sunwoo) 大韓微生物學會 1993 大韓微生物學會誌 Vol.28 No.5

Phytol과 들미나리추출물이 Sarcoma 180마우스의 T Subset에 미치는 효과

김광혁(Kwang-Hyuk Kim),장명웅(Myung-Woong Chang),박건영(Kun-Young park),이숙희(Sook-Hee Rhee),류태형(Tae-Hyong Rhew),선우양일(Yang-ll Sunwoo) 한국식품영양과학회 1993 한국식품영양과학회지 Vol.22 No.4

본 연구는 녹황색채소류에서 추출되어 항암효과를 나타내는 활성물질로 보고 되어 있는 phytol과 들미나리추출물을 sarcoma 180 마우스에 주사한 후 적출한 비장세포내 T임프구와 T subset, 그리고 asiaol GM^1 세포를 정량하여 다음과 같은 결과를 얻었다. 1) 종양 마우스에 phytol을 투여하였을 때 비장세포내의 T cell과 T-subset은 종양세포이식에 의해서 상승된 치를 더욱 증가시켰다. 그러나 들미나리추출물의 경우는 대동소이하였다. 2) Asialo GM1^+ 세포는 종양마우스에 phytol이나 들미나리추출물을 주사하였을 때 모두 상승하였으며 정상마우스에 phytol을 투여하였을 때도 대조군에 비하여 상승했지만 들미나리추출물을 작용시켰을 때는 저하되었다. 3) L3T4^+/Lyt-2^+세포비는 종양마우스에 phytol을 주사하였을 때 감소를 보였지만 정상 마우스에 투여하였을 때는 더욱 크게 낮아졌다. 그러나 들미나리추출물을 정상마우스에 투여하였을 때는 크게 감소하던 것이 종양마우스에 적요 시켰을 때는 증가현상을 보였다. 이상의 결과로 미루어 볼 때 phytol이나 들미나리추출물은 종양마우스에서 작용자 세포인 자연살해세포(natural killer cell)의 활성인자로서 작용할 가능성이 높을 것으로 사료된다. Several studies have shown that phytol isolated from perilla leaf and small water dropwort (Oenanthe javanica(Bl) D.C., wild type) extract reveal antitumor activities. In the present study we investigated the effect of phytol and the small water dropwort extract on the T subset in the sarcoma 180-transplanted mice in order to elucidate the immunological mechanism of antitumor activity of these substances. The results obtained from the experiment were as follows: When phytol was injected into the sarcoma-180 transplanted mice (control), the levels of T cell and T subset by inoculation of the tumor cells were enhanced, but that of T cells in case of small water dropwort extract was similar to the control. Asialo GM1^+ cells were increased when phytol or small water dropwort extract was injected into tumor-transplanted mice. In normal mice the number of asialo GM1^+ cells increased with phytol injection and decreased with small water dropwort extract injection. L3T4^+ ILyt-2^+ cell ratios were decreased when phytol was injected into tumor-transplanted mice, but increased in case of small water dropwort extract injection. In normal mice the ratios showed large decreases with phytol or small water dropwort extract injection. These results indicate that phytol or small water dropwort extract can activate the proliferation of natural killer cells that are effector cells in tumor-bearing mice.

들깻잎에서 동정한 Phytol의 항암 및 면역활성증강 효과

김광혁(Kim Kwang Hyuk),장명웅(Chang Myung Woong),박건영(Park Kun-Young),이숙희(Rhee Sook-Hee),류태형(Rhew Tae Hyong),선우양일(Sunwoo Yangil) 韓國營養學會 1993 Journal of Nutrition and Health Vol.26 No.4

Antimutagenic Effects and Compounds Identified from Hexane Fraction of Persimmon Leaves

문숙희(Suk-Hee Moon),김정옥(Jeong-Ok Kim),이숙희(Sook-Hee Rhee),박건영(Kun-Young Park),김광혁(Kwang-Hyuk Kim),류태형(Tae-Hyong Rhew) 한국식품영양과학회 1993 한국식품영양과학회지 Vol.22 No.3

감잎의 메탄올 추출물이 Salmonella typhimurium TA100에서 aflatoxin B₁(AFB₁), DMAB, MNNG, 그리고 4-NQO의 돌연변이 유발성을 억제시키는 효과가 있었다. 메탄올 추출물을 다시 hexane, chloroform, ethyl acetate, butanol, 그리고 수용성층으로 분획하여 각 획분의 수득율과 항돌연변이 효과를 조사하였다. Hexane, butanol, 그리고 수용성 획분의 수득율이 높았으며, 이중 hexane 획분이 AFB₁, DMAB, MNNG, 그리고 4-NQO에 대한 Salmonella typhimurium TA100에서 항돌연변이효과가 가장 크게 나타났다. Hexane 획분을 silica gel column과 thin layer chromatography(TLC)법으로 연속분리하여 TLC상에서 8개의 bands로 분리하였다. 그중 항돌연변이 효과가 가장 컸던 band를 hexane/ethylacetate(1 : 1, v/v)로 추출한 다음 그중에 존재하는 화합물을 GC-MS를 이용하여 분리 동정하였다. 활성획분에서는 1'-oxocannabinol, 3β-acetoxy-17-methyl-5α-18(13-17) abeoard-rost-13-ene, 4-methoxy-2' 6' -dinitro-3,5-di-t-butylbiphenyl, 8,9-dihydro-5,6-dimethoxy-dibenz[c,h] isoquino [2,1,8-1ma] carbazole-11, 16-dione 등이 분리동정되었다. Methanol extract of dried persimmon leaves was fractionated to hexane, chloroform, ethyl acetate, butanol, and aqueous fractions. Hexane, butanol, and aqueous fractions had high yields of extracts. Hexane fraction among these fractions showed the highest inhibition rate on the mutagenicities of aflatoxin (AFB₁), dimethyl-amino-biphenyl (DMAB), N-methyl-N' -nitro-N-nitrosoguanidine (MNNG), and 4-nitroquinoline-1-oxide (4-NQO) in Salmonella typhimurium TA100. Hexane fraction was further fractionated into eight fractions by silica gel column chromatography and thin layer chromatography (TLC). The fraction 5 on TLC exhibited the highest antimutagenic activity on AFB₁, DMAB, and MNNG. 1'-oxocannabinol, 3B-acetoxy-17 -methyl-5a-18(13-17) abeoardrost-13-ene, 4-methoxy-2' 6'-dinitro-3,5-di-t-butylbiphenyl, 8, 9-dihydro-5, 6-dimethoxy-dibenz[c, h]isoquino [2, 1, 8-1ma]carbazole-11, 16-dione were tentatively identified from this antimutagenic fraction by GC-MS.

Depletion of Extra-Hepatic Tissue Carnitine : A Source of Increased Hepatic Carnitine in Chronic Alcoholid Rat Chronic Alcoholic Rat에 있어서 Hepatic Carnintine 증가의 원인

Rhew, Tae Hyong 부산대학교 1990 자연과학논문집 Vol.49 No.-

Male Sprague-Dawley rats were fed nonacoholic or alcoholic diets as such or supplemented with carnitine or its precursors for 56 days. Blood and tissues were collected and analyzed for carnitine and acylcarnitine by a radioisotopic procedure. While carnitine concetrations were higher than normal in alcohol compromised liver, they were consistently lower than normal in most of the extrahepatic tissues. The most significant carnitine depletion was found in the skeletal and cardiac muscle. All tissues were repleted by supplementary carnitine, however, carnitine precursors were not so efficacious. It is concluded that chronic alcoholic liver sequestered carnitine at the expense of extrahepatic tissues. Indexing key Words: Chronic alcoholism, tissue-carnitine, acylcarnitine, supplementary carnitine, carnitine depletion, carnitine repletion, carnitine precursors. Caritine is essential for the translocation of fatty acids among organs and organelles(1). Its supply in healthy mammals is ensured by endogenous biosynthesis from lysine and methionine as well as via ditary intake(2). In the rat, carnitine is biosynthesized primarily in the liver and distributed to other organs(3). Liver carnitine concentrations have been shown to increase in a number of pathophysological conditions such as starvation(4,5), diabetes mellitus(6) and clofibrate treatment(7). In addition an increase in hepatic carnitine concentration has been observed in chronic alcoholic rats(8-11): however, the mechanism of its accumulation has not been elucidated. The evidence is presented to support the hypothesis that increased carnitine concetraations in an ethanol-compromised liver may have resulted from sequestration of carnitine from plasma at the expense of extrahepatic tissues.

Effects of Myo-inositol and Riboflavin on Ethanol-Induced Fatty Liver and Hyperlipidemia in Rats

Rhew, Tae Hyong,Chun, Jang Soo 부산대학교 1987 자연과학논문집 Vol.44 No.-

sz12/ Ethanol이 지방대사에 미치는 영향중에서 가장 특징적인 것이 과유지방혈증과 지방간에서부터 간경변에 이르는 간의 증상들인바, 본 실험에서는 diet 총 열량의 36%에 해당되는 ethanol을 실험용 흰쥐에 45일간 투여해서 ethanol유발성 지방간과 과유지방혈증을 일으키고 myo-inositol과 riboflavin의 처리에 따른 그 치료효과를 고찰하였다. Ethanol diet에 2.5mg/100g body wt.의 myo-inositol을 처리한 흰쥐에서는 간과 plasma에서 모든 종류의 지질들이 현저히 감소하였고, riboflavin(6.6mg/200g body wt.)을 처리한 쥐에서는 단지 간에서 Total lipid, 중성지질, 인지질 그리고 콜레스테롤의 양만이 현저히 감소되었다. The lipid-lowering effect of Myo-inositol and Riboflavin was examined in male Sprague-Dawley rats fed ethanol as 36% of the total calories for a duration of 45day, Ethanol produced typical hepatic steatiosis and hyperlipidemia characterized by significantly elevated levels of total lipid, triglycerides, phospholipid, and free fatty acids. Supplementation of ethanol diet with riboflabin(6.6mg/100g body weight) significantly lowered ethanol-induced increases of the various lipid classes such as total lipid, triglycerides, anf total cholesterol in liver, but reduction in plasma lipid classes was minimal. The effect of Myo-inositol appeared to be superior to that of riboflabin. Myo-inositol significantly reduced ethanol-induced elevation of all classes of lipids in livers and plasma.