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Effect of Paclitaxel on the β-actin, Fibronectin, Laminin and Fine Structure in HeLa and L929 Cells
김주영(Joo-Young Kim),류인(Yin Liu),성훈기(Hoon-Ki Sung),박정현(Jeong-Hyun Park),송인환(In-Hwan Song),성언기(Eon-Gi Sung),이융창(Yungchang Lee) 대한해부학회 2001 Anatomy & Cell Biology Vol.34 No.1
Paclitaxel로 처리한 HeLa 및 L929 암세포주에서 생기는 β-actin, fibronectin 및 laminin의 세포질 내 분포의 변화가 형태학적인 변화와는 어떤 연관이 있는지를 알아보기 위해 본 실험을 시행하였다. 이 약제에 의한 세포증식의 조절에 관하여는 MTT 분석을 하였고, 위에서 언급된 세포질 내 단백질들의 변화는 면역세포화학적 방법과 analySIS AUTO로 분석하였다. 세포의 형태적인 변화들은 도립현미경과 투과전자현미경으로 관찰하였다. MTT 분석 결과로는 paclitaxel 1 μM과 10 μM의 농도에서 HeLa 세포는 세포성장이 대조군에 비해 20~80% 억제되었고 L929 세포는 27~44% 억제되었으며, 그 효과는 약제의 용량과 시간에 따라 증가되었다. β-actin, fibronectin 및 laminin의 분포는 핵 주변부에서 강한 반응을 보였던 것이 약제 처리 후 세포질 전체에 고루 분포하면서 약한 반응을 나타냈다. 형태학적인 변화로는 2가지 종류의 세포 모두에서 이질염색질의 증가, 과립형질내세망의 부착리보소체의 떨어짐 및 골지복합체의 절편화를 쉽게 볼 수 있었다. 따라서, paclitaxel에 의한 이들 세포의 성장억제와 형태학적인 변화들은 위의 세가지 종류의 단백질들의 재배열과 그 합성의 감소와 밀접히 연관될 것으로 생각된다. The aim of this investigation was to elucidate the changes in the cytoplasmic distribution of β-actin, fibronectin, and laminin through mainly the morphological changes occurring in HeLa and L929 cancer cell treated with paclitaxel. Whether or not paclitaxel regulates cell proliferation was assessed with MTT assay. Possible influence on the distribution of β-actin, fibronectin, and laminin in these cells was confirmed with immunocytochemistry and analySIS Auto software program. The changes in cell morphology were observed under inverted and electron microscope. The MTT assay showed that 1 μM and 10 μM concentrations of paclitaxel inhibited HeLa cell growth approximately by 20% to 80% and L929 cell by 27% to 44% in a dose- and time-dependent manner. The distribution of these proteins was changed from the condensed around nucleus and strong reaction to the weak throughout cytoplasm. The morphological changes indicated that paclitaxel changes the location or number of protein synthesis apparatus: damages of RERs, Golgi complex, and increase of heterochromatin. These data suggest that the growth inhibition and morphological changes of tumor cells induced by paclitaxel might be modulated by the rearrangement and decreased production of β-actin, fibronectin, and laminin in cytoplasm.
녹차의 에탄올 추출물이 암세포주의 형태학적변화에 마치는 영향
장원태 ( Won Tae Jang ),이효권 ( Hyo Kwon Lee ),김미형 ( Mi Hyung Kim ),류인 ( Yin Liu ),최원경 ( Won Kyung Choi ),성훈기 ( Hoon Ki Sung ),박정현 ( Jeong Hyun Park ) 대한임상검사과학회 2000 대한임상검사과학회지(KJCLS) Vol.32 No.3
Green tea is one of most popular traditional teas in korea. ``The purpose of this study was comparing the effects of ethanol extracts on three kinds of cancer cell lines(HepG2, A549, EATC) according to its concentration and exposure time. For this purpose, we obseπed morphological changes under inverted and electron microscope and anallysed cytotoxicity . EATC(Ehrlich-Lettre Ascites Tumor Cell) cells were damaged by 100μg/m1 ethanol extracts treatment until 72 hours gradually. In A549 cells, ethanol extracts caused severe cellular injury abrupt1y and lasted for 72 hours. However, cellular necrosis and growth inhibition in both of them could not be observed in 1, 10μg/ml ethanol extracts administration. Whereas, the effects of that were weak in all of concentrations and exposure times in HepG2 cells. In conclusion, green tea ethan이 extracts induced strong inhibition of growth and severe cellular damages in A549(Lung Cancer) and EATC except HepG2(Hepatoblastoma). In addition, its effects were dependent on concentration and duration of ethanol extracts. Therefore, though there were some different effect according to cancer type, it suggested that green tea would be helpful to prevent carcinogenesis and treat cancer.