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Sprague-Dawley 랫드를 이용한 Up & Down 법 (UDP)에 의한 LMK02의 단회 경구투여 독성시험
강형원 ( Hyung Won Kang ),권영미 ( Yeong Mi Kwon ),이상원 ( Sang Won Lee ),김지훤 ( Ji Hwon Kim ),이효경 ( Hyo Gyung Lee ),장현호 ( Hyun Ho Jang ),박보라 ( Bo Ra Park ),류영수 ( Yeoung Su Lyu ) 대한한방신경정신과학회 2010 동의신경정신과학회지 Vol.21 No.3
Objectives: The oriental medicine Jangwonhwan originally described in the Korean medical text, DonguiBogam(amnesia chapter). Recently, a modified formula of Jangwonhwan(LMK02-Jangwonhwan), was shown to reduce β-amyloid deposition in the brain of Tg-APPswe/PS1dE9 mouse model for Alzheimer`s disease. This experiment aimed to investigate the acute oral toxicity of LMK02 in SD rats by up-and-down procedure determinations. Methods: Quality control of tablet form of LMK02 was established by estimating indicative components, Ginsenoside Rg3 of Red Ginseng and Decursin of Angelicagigas Nakai. The toxicity of LMK02 was investigated in 6 week old, specific pathogen free(SPF), Sprageu-Dawley rats. 3 female rats received 5,000 mg/10 ml/kg of test substance and their death rate, clinical sings, weight changes and autopsy findings had been observed for 2 weeks. Results: Any specific symptoms or death were resulted in this experiment. No significant changes in rats` weight. No significant differences in atopsy. Conclusions: The minimum lethal dose(MLD) of LMK02 for female Sprauge-Dawley rats were more than 5,000mg/kg in this experiment.
불면 처방에 관한 문헌고찰 -동의보감(東醫寶鑑) 몽문(夢門)을 중심으로
박보라 ( Bo Ra Park ),박인숙 ( In Sook Park ),강형원 ( Hyung Won Kang ),류영수 ( Yeong Su Lyu ) 대한한방신경정신과학회 2011 동의신경정신과학회지 Vol.22 No.2
Objectives: By analyzing and comparing indications, components and their doses of herb medicines, which were recorded in insomnia chapters of medical books, we examined historical changes and made clear documentary ground of medications. Methods: 1. 10 books which were noted in insomnia part of 『The Eastern Medical Textbook of neuropsychiatry』 and 13 books were mentioned in same part of 『Uibujeonrok』 were selected as reference. 2. We searched article database such as KISS, riss4U and journals issued by the Korean society of oriental neuropsychiatry. Finally, 39 books which were noted in 3 literature reviews, were chose as reference. 3. In reference books, we collected the herb medicines in chapters related to insomnia. 4. In case of no section associated with insomnia in books, we found prescriptions related to insomnia in whole chapters. 5. If the chief virtue of medication was not primarily for insomnia, we excluded applied or added herb medicines. 6. We classified prescriptions into small groups which had same name, and then rearranged them in chronological order. Results and Conclusions: 1. Banha-tang( banxiatang) of 『Hwangjenaegyeong·Youngchu』 was the first-recorded prescription used for insomnia. 2. The chapter of insomnia appeared in 『Chunkeumyobang』 at first. 3. Ondam-tang(Wendantang) was most cited prescription in medical books. It was recorded totally 19 books from 『Chunkeumyobang』 to 『Hyeoljeungron』. 4. We suspects indications, components, dosage of medication of insomnia in Donguibogam were revised according to author`s clinical experience and circumstances.
치매(痴매)에 대한 최신 실험적 연구 동향 (2000년 이후 한의학 학술지를 중심으로)
최성열 ( Sung Youl Choi ),김대현 ( Dae Hyun Kim ),김상태 ( Sang Tae Kim ),김태헌 ( Tae Heon Kim ),강형원 ( Hyung Won Kang ),류영수 ( Yeong Su Lyu ) 대한한방신경정신과학회 2008 동의신경정신과학회지 Vol.19 No.1
Objectives : The purpose of this study is to suggest for the following experimental study of dementia by reviewing recent oriental medicine journals that have been published since 2000. Methods : We have investigated various types of studies in relation to dementia through 90 articles that have been published from 2000 to 2007 in recent oriental medicine journals were registered Korea research foundation. Results and Conclusions : 1. Since 2000, 88 articles in relation to dementia have been published and almost of them are herbal medicine-centered studies. Also they show a tendency to increase every year. The journal of oriental neuropsychiatry carries the highest number of studies in relation to dementia. 2. According to the experimental paper, there are 30 cases of using herb simplexes, 48 cases of herb-combined prescription, and 10 cases of other ways. Especially 7 cases of using herb-combined prescription relation to Sasang constitution are all for the Taeumin. 3. There are 85 cases of Animal and cellular experimental, 60 cases of using pathologic model induced cytotoxic activity, a case of using L-NAME, 3 cases of 192 saporin, 4 cases of ibotenic acid, 10 cases of focal cerebral ischemia, 3 cases of alcohol-administered, and one case of natural degradation. 4. Morris water maze, Radial arm maze Passive avoidance learning model were using for examining learning and memory of model animal 5. We propose that following studies of dementia are to be investigated of the applied method of using siRNA with tranceduced gene, sample preparation by water-soaking, oriental medical diagnosis, standardization of differentiating symptom and herb simplexes, building the database by classified prescriptions, and experiment model which are based on precise examining mechanism with cell line as like mouse H19-7 hippocampus, rat HT22 hippocampus, astrocyte, microglia, using the model of animals at APP, PS1, BACE, CT99/PS1, APOE4, Tau, APP/PS1/Tau
울증(鬱證)환자에게 용서프로그램을 활용한 오지상승요법(五志相勝療法)을 시행하여 호전된 1례
이상언 ( Sang Eon Lee ),노동진 ( Dong Jin No ),박장호 ( Jang Ho Park ),이고은 ( Go Eun Lee ),박인숙 ( In Sook Park ),류영수 ( Yeong Su Lyu ),안민섭 ( Min Seob Ahn ),정지호 ( Ji Ho Jung ) 대한한방신경정신과학회 2010 동의신경정신과학회지 Vol.21 No.2
Yuzheng(鬱證) comes from obstruction of qi by stress. The patient has depressed mood, irritable sign, chest discomfort, costal pain, angry state or some strange feeling on the throat. Oh-Ji-Sang-Seung(五志相勝) therapy is base on the theory of interrelation in five elements in oriental medicine. The contents of Oh-Ji-Sang-Seung(五志相勝) therapy include five subjugations of five emotions. Anxiety subjugates fear(思勝恐), fear subjugates joy(恐勝喜), joy subjugates pity(喜勝悲), pity subjugates anger(悲勝怒), and anger subjugates anxiety(怒勝思). Forgiveness program is a kind of psychological therapies to decrease the degree of anger and it is included in Oh-Ji-Sang-Seung(五志相勝) therapy. In this case, a female patient, 50 years old, who suffered from Yuzheng(鬱證) with chest discomfort, irritable sign, easily angry state, depressed mood, hot flush, insomnia. We used Oh-Ji-Sang-Seung(五志相勝) therapy besides herbal medication, acupuncture to her condition got improved. Therefore we reported it for the treatment.
류승준 ( Seung Jun Ryu ),이충식 ( Chung Sik Lee ),박보라 ( Bo Ra Park ),서종훈 ( Jong Hoon Seo ),강형원 ( Hyung Won Kang ),류영수 ( Yeong Su Lyu ),김태헌 ( Tae Heon Kim ) 대한한방신경정신과학회 2007 동의신경정신과학회지 Vol.18 No.1
Objective : The aim of this study was to investigate effects of Gagambosim-Tang(GBT) of focal brain ischemic injury induced by intraluminal filament insertion in the rats.Method : The ischemia was induced by intraluminal filament insertion into middle cerebral artery. Eight-arm radial maze was designed for the behavioral task. Gagambosim-Tang was orally administrated in SD rats for 21 days. The task was started on the 4th week after focal brain injury, and performed two trials per day for 6days. The effects of Gagambosim-Tang on neuroprotective agents in cresyl violet, choline acetyltranferase(ChAT), nerve growth factor (NGF)-stain and c-Fos with ischemic injury were investigated.Results : The errors in the eight-arm radial maze task were significantly decreased in normal group compared with control group on 2-6days, GBT 1X(42.2 mg/ml)orally administrated group on 1days, GBT 6X(253.2 mg/ml) on 3, 5, 6days. The rate of correct choice was increased in normal and GBT 6X groups. The neuroprotective effect in the hippocampal CA1 was increased in normal and GBT 1X, GBT 6X groups compared with control group. The density of ChAT in the hippocampal CA1 was increased in normal and GBT6X groups compared with control group. The density of NGF in the hippocampal CA1 was increased normal and GBT6X groups compared with control group. The number of c-Fos-positive neurons in the hippocampal CA1 was increased in normal and GBT 6X groups compared with control group.Conclusion : These results suggest that Gagambosim-Tang may have protective effect on dementia.
柳泳秀,姜亨源,李光揆,崔用德 대한동의병리학회 1999 동의생리병리학회지 Vol.13 No.1
전 세계적으로는 1700만에서 2500만명이 알츠하이머병 환자가 있고 실제로 85세이상의 인구중 약 50%가 알츠하이머병인 것으로 알려져 있다. 알츠하이머형 치매의 경우 腦에서 大腦皮質의 萎縮, 副交感神經과 다른 神經細胞의 退化, neurofibrillary tangles(신경원섬유농축제, NFTs)의 存在, senile plaques(노인반)의 蓄積과 같은 4가지 구조적변화가 나타나며 neurofibrillary tangles와 senile plaques는 알츠하이머형 치매의 지표로 인식되고 있다. 그러나 지금도 알츠하이머병에 대한 發病原因이 不分明하여 硏究를 계속 進行 중이다. 특히 腦의 構造에서 非正常的으로 발견되는 두 가지 構造인 neurofibrillary tangles와 senile plaques가 무엇이며, 이러한 구조가 어떻게 만들어졌는가를 중심으로 활발히 硏究가 이루어지고 있다. 이에 著者는 이미 腦 代表的인 退行性 疾患으로 알려진 알츠하이머형 치매에 나타난 病理的 所見과 最近 發表된 硏究를 中心으로 韓醫學的인 病因病機를 比較 分析하였다. 痴매의 老化와 遺傳的 要因이 韓醫學의 臟腑的으로는 腎虛와 밀접한 關係를 맺고, 베타-아밀로이드와 tau 단백질과 같은 뇌속의 비정상적인 plaque는 痰濁과 瘀血 등의 韓方的 病理産物과 유사한 의미를 가지므로 실제 임상에 있어서도 베타-아밀로이드와 tau 단백질을 除去하는 서양의학적인 原因除去 治療法과 韓醫學的인 腦와 五臟과 關係에서 補腎法과 祛痰化瘀法을 竝行한다면 보다 나은 효과가 있을 것으로 思料된다. Through study of eastern and western records of dementia, the result were obtained as follows ; 1. The chief expression and pathogenic change of alzheimer's disease, the typical degenerative disease of brain, is overall cerebrum atropy, cerebral ventricular enlargement, senile plaque and neurofillary tangles(NFTs) etc. 2. The etiology of dementia is genetic factors, immunologic factor, virus, toxic substance and cephalic trauma etc. Among the rest, the genetic factor is being studied vigorously. 3. The typical substance of expired dementia brain cell are β-amyloid and tau protein. The accumulation of β-amyloid is known as the cause of senile plaque and amyloid angiopathy. The neurofillary tangles are known as substance made through the process of hyperphosphate of tau, a protein of neuronal cell frame. 4. From the oriental medical point of view, the defficiency of the kidney, liver and heart is the basic internal cause of dementia DamTak(痰濁), Blood stasis(瘀血), Chiljung(七情), Fung(風) and Hua(火) are exterior cause factor. Among the rest, the defficiency of the kidney is the most cause factor. 5. From the oriental medical point of view, the aging and genetic factor of dementia have much connection with the defficiency of kidney. Abnormal plaques of the brain such similar to the estern pathogenic substance such as Domtak(痰濁) and Blood stasis(瘀血). Like this, as the result of the study about the pathogenic subsntce of dementia is being studied vigorously, to treat dementia, etiologic factor-β-amyloid, tau protein-removal therapy and the eastern Bushen(補腎) therapy and Qutanhuayu(祛痰化瘀) therapy is likely to help the better clinical application.
釣鉤藤이 酸素自由基에 의하여 損傷된 培量脊髓感覺神經節細胞에 미치는 影響에 關한 硏究
강형원,박진성,류영수,Jin Mo Chung,이건목 대한한방신경정신과학회 2000 동의신경정신과학회지 Vol.11 No.1
To study the effects of Ramulus et Uncus Uncariae (REUU) on oxygen free radical-mediated damage by hydrogen peroxide (H-2O-2) on cultured spinal sensory neurons, in vitro assays such as MTT assay, NR assay, neurofilament enzymeimmuno assay (EIA), sulforhodamine B (SRB) assay, assay for lactate dehydrogenase (LDH) activity and assay for lipid peroxidation were used in cultured spinal dorsal root ganglion neurons derived from mice. Spinal dorsal root ganglion neurons were cultured in media containing various concentrations of H-2O-2 for 5 hours, after which the neurotoxic effect of H-2O-2 was measured by in vitro assay. The protective effect of the herb extract, Ramulus et Uncus Uncariae (REUU) against H2O2-induced neurotoxicity was also examined. The results are as follows. 1. In NR assay and MTT assay, H-2O-2 sigruficantly decreased the cell viability of cultured mouse spinal dorsal root ganglion neurons according to exposure concentration in these cultures. An additional time course study was done on these cultures. 2. Cultured spinal dorsal root ganglion neurons which were exposed to various concentrations of H-2O-2 showed a quantitative decrease of neuronal cells by EIA and of total protein by sulforhodamine B (SRB) assay, while they showed an increase of both lipid peroxidation and LDH activity. 3. The effect of Ramulus et Uncus Uncariae (REUU) on H-2O-2 induced neurotoxicity showed a quantitative increase in both neurofilament and total protein, but showed a decrease of lipid peroxidation and LDH activity. These results suggest that H-2O-2 has a neurotoxic effect on cultured spinal dorsal root ganglion neurons from mice and that the herb extract, Ramulus et Uncus Uncariae (REUU), was very effective in protecting H-2O-2 induced neurotoxicity by decreasing lipid peroxidation and LDH activity. “This work was supported by the Brain Korea 21 Project.”