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Cytokinin과 대두(Glycine max)잎단백질의 결합에 대하여
류기중,박창규,정창조 제주대학교 방사능이용연구소 1986 연구보고 Vol.2 No.-
cytokinin과 단백질의 결합을 간단하게 검정하는 방법으로서 전기영동법을 시도하고, 대두의 잎단백질과 cytokinin의 결합여부, cytokinin에 대한 affinity가 있는 단백질의 종류와 상대적 affinity를 조사하였다. 검토된 전기영동법은 cytokinin과 단백질의 결합 뿐만아니라, cytokinin에 대한 상대적 affinity를 동시에 검정할 수 있는 장점을 가지고 있었다. Ammonium sulfate 침전법, Sephadex G-25 chromatography, paper chromatography, 그리고 전기영동으로 대두의 잎단백질 중에 BA와 결합하는 단백질이 있음을 확인할 수 있었다. 전기영동법으로 검정한 결과 BA와 결합하는 것이 3 group이 있고, 이중에서 전기영동 이동도로 보아 분자량이 작은 단백질 분획과 전기영동 이동도 0.4부근의 분획은 BA에 대한 affinity가 비교적 낮은 반면, 이동도 0.0∼0.2의 분획은 affinity가 큰 것으로 생각되었다. A polyacrylamide gel electrophoresis technique was applied to cytokinin-protein binding assay. Binding of soybean leaf proteins to cytokinin and relative affinities of protein fractions to cytokinin were studied. The electrophoresis technique appeared to be very useful for determination of cytokinin-protein binding, for identification of protein species binding to cytokinin, and for comparison of relative affinities of the proteins to cytokinin. The presence of cytokinin-binding proteins in soybean leaves was confirmed from assays with ammonium sulfate precipitation, Sephadex G-25 chromatography, paper chromatography, and electrophoresis. Three groups of cytokinin-binding proteins were identified in the soybean leaf protein extract and two of the three showed low affinity to cytokinin, however, the third one with mobility between 0.0∼0.2, probably high molecular weight protein (s), showed high affinity in the electrophoretic analysis.
대두 Protoplast 세포벽 합성과 세포분열에 대한 Cytokinin 의 영향
김창오,류기중,김형옥,박창규 제주대학교 방사능이용연구소 1988 연구보고 Vol.3 No.-
대두 (Glycine max var. Acme )의 자엽조직에서 유래된 callus의 부유배양 세포로부터 protoplast를 분리하고, 이들 protoplast 의 viability, 세포벽재생 및 세포분열에 대한 benzyladenine (BA)의 영향과 protoplast에 의한 BA의 흡수특성을 조사하였다. Protoplast 의 viability 는 BA처리에 의하여 증가되었고, 세포벽재생과 세포분열 그리고 callus의 생장도 BA처리에 의하여 촉진되었다. Protoplast에 의하여 흡수된 BA의 양은 BA처리후 약 20시간에 최대에 이르렀고, 이중에서 약 2/3가 6시간 이내에 흡수되었다. Effects of benzyladenine (BA) on viability, cell wall regeneration and division of soybean (Glycine max, Var. Acme) protoplats isolated from suspension cells of cotyledonary callus were investigated. The uptake of BA by the protoplasts was also studied. BA increased protoplast viability, and promoted cell wall regeneration and cell division. The level of BA in protoplasts was increased to a maximum at about 20 hours incubation 2/3 of the total amount of BA accumulated in protoplast was absorbed within 6 hours.
Cytokinin 과 대두(Glycine max) 잎단백질의 결합에 대하여
박창규,류기중,정창조 한국농화학회 1986 Applied Biological Chemistry (Appl Biol Chem) Vol.29 No.1
A polyacrylamide gel electrophoresis technique was applied to cytokinin-protein binding assay. Binding of soybean leaf proteins to cytokinin and relative affinities of protein fractions to cytokinin were studied. The electrophoresis technique appeared to be very useful for determination of cytokinin-protein binding, for identification of protein species binding to cytockinin and for comparison of relative affinities of the proteins to cytokinin. The presence of cytokinin-binding proteins in soybean leaves was confirmed from assays with ammonium sulfate precipitation, Sephadex G-25 chromatography, paper chromatography, and electrophoresis. Three groups of cytokinin-binding proteins were identified in the soybean leaf protein extract and two of the three showed low affinity to cytokinin, however, the third one with mobility between 0.0∼0.2, probably high molecular weight protein (s), showed high affinity in the electrophoretic analysis.
대두 Protoplast 의 세포벽 합성과 세포분열에 대한 Cytokinin 의 영향
박창규,류기중,김형옥,김창오 한국농화학회 1987 Applied Biological Chemistry (Appl Biol Chem) Vol.30 No.4
Effects of benzyladenine (BA) on viability, cell wall regeneration and division of soybean (Glycine max, Var. Acme) protoplasts isolated from suspension cells of cotyledonary callus were investigated. The uptake of BA by the protoplasts was also studied. BA increased protoplast viability, and promoted cell wall regeneration and cell division. The level of BA in protoplasts was increased to a maximum at about 20 hour incubation and 2/3 of the total amount of BA accumulated in protoplast was absorbed within 6 hours.
당근 세포의 단백질 생합성에 대한 몇가지 식물생장조절제의 영향
박창규,류기중,송태철 한국농화학회 1985 Applied Biological Chemistry (Appl Biol Chem) Vol.28 No.3
Electrophoretic studies of protein extracts from carrot calluses suspension-cultured on the media containing kinetin, BA, IAA, NAA or GA₃ at the levels of 10^(-6), 10^(-5), 10^(-4)M, respectively, were performed to identify polypeptides and proteins regulated by auxin, cytokinin or GA. Fifteen bands of polypeptide(s) were observed in the callus cultured in the control medium devoid of growth regulators, and their molecular weights were 18.₄, 20.₂, 20._0, 34._9, 35._7 37.₄ 40.₃, 44.₁, 44.₄, 44.₄, 49.₃, 55._0, 56._6, 58.₁, and 59._9 KD, respectively. The synthesis of polypeptide appeared to be promoted in two bands by kinetin, in six bands by BA, in one band by IAA, in two bands by NAA, and in four bands by GA₃, while inhibited in five bands by kinetin, in three bands by BA, in four bands by IAA, in three bands by NAA and in three bands by GA₃. The polypeptides of 40.₃ KD and 42.₂ KD seemed to be regulated by cytokinins, and those of 44.₁, KD, 37.₄ KD, and 56._6 KD by auxins. The proteins of three bands with relative mobilities of 0.56, 0.84, and 0.92, respectively, increased in the calluses cultured on the media containing kinetin, IAA, GA₃, NAA or BA, compared to the control, but it was difficult to identify the proteins specific for each growth regulator.
Auxin , GA 및 Cytokinin 이 대두의 단백질합성(축적) 에 미치는 영향
박창규,김수일,류기중 한국농화학회 1986 Applied Biological Chemistry (Appl Biol Chem) Vol.29 No.1
Aqueous solutions of 2, 4-D, BA or GA₃(10^(-6), 10^(-5), and 10^(-4)M, respectively) were sprayed onto soybean (Glycine max) plants in the flowering stage, and proteins of immature (33days after flowering) and mature (77days after flowering) seeds were analyzed by electrophoresis to elucidate the effects of the growth reguators on protein synthesis or protein accumulation in the seeds. Accumulations of some proteins were altered by 2, 4-D or BA at certain concentrations, but no proteins were affected by GA₃. The α and α' subunits of 7S and acidic subunit of 11S disappeared in mature seeds after treatments at the flowering stage with 2, 4-D or BA. The presence of α and α' subunits of 7S and acidic subunit of 11S in immature seeds indicated that the absence of the above polypeptides in mature seeds did not result from inhibitions in syntheses of the polyeptides by the growth regulators. Disappearance of the above proteins in mature seeds seemed to be concerned with the action of specific proteolytic enzymes) (metalloendopeptidase?), and 2, 4-D and BA might promote gene expression or activation of the enzyme.