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초두구로부터 분리한 비환식 트리테르페노이드계 화합물의 세포 접착인자 저해활성
김지수,노문철,김강성,김관희,임병용,Kim, Ji-Soo,Rho, Mun-Chual,Kim, Gang-Seong,Kim, Koanhoi,Rhim, Byung-Yong 한국해양바이오학회 2007 한국해양바이오학회지 Vol.2 No.4
The present study purified two noncyclic triterpenoids, compound 1 and compound 2, that intervene interaction of ICAM-1 and LFA-1 from ethanol extracts of Alpinia katsumadai. The compound 1 and 2 inhibited adherence of sICAM-1 to THP-1 cells with an $IC_{50}$ of $7.59{\mu}g/m{\ell}$ 및 $6.98{\mu}g/m{\ell}$, respectively, without affecting viability of the cells. The compound 1 and 2 also inhibited interaction of CHO-ICAM-1 cells with THP-1 cells with an $IC_{50}$ $6.7{\mu}g/m{\ell}$ 및 $5.5{\mu}g/m{\ell}$, respectively. These findings suggest that the noncyclic triterpenoids from Alpinia katsumadai have inhibitory activities against cell adherent molecules. The present study proposes that noncyclic triterpenoids from Alpinia katsumadai can applied to therapeutic approaches to the diseases that are associated with adhesion of inflammatory cells. 1. 천연자원으로부터 ICAM-1과 LFA-1 매개성 세포접착 저해제를 탐색한 결과, 초두구 에탄올 추출물은 이에 대한 저해 활성을 나타내었다. 2. 초두구로부터 세포 접착 인자 활성에 관한 물질을 분리 정제하기 위하여, 각종 용매추출과 컬럼 크로마토그래피를 이용하여 얻은 화합물 1과 2로 세포접착인자 저해활성을 확인하였다. 3. 분리된 화합물 1과 2의 sICAM-1과 THP-1 세포와의 결합저해활성 측정 결과, $IC_{50}$ 값은 각각 $7.59{\mu}g/m{\ell}$ 및 $6.98{\mu}g/m{\ell}$로 측정되었다. 실험에 사용된 농도에서는 세포독성을 보이지 않았다. 4. 분리된 화합물 1과 2의 CHO-ICAM-1과 THP-1 세포간의 결합저해활성을 측정한 결과, $IC_{50}$ 값이 각각 $6.7{\mu}g/m{\ell}$ 및 $5.5{\mu}g/m{\ell}$로 측정되었다. 5. 이상의 실험 결과로 미루어 보아, 초두구로부터 분리한 비환식 트리테르페노이드계 화합물들은 세포 접착인자에 대한 저해활성을 가지고 있다고 볼 수 있으며 이 기전을 통하여 생길 수 있는 질환에 대한 치료제 개발에 유효할 것이다. 특히 동맥경화에 대해서는 새로운 기전으로 접근하고 있으므로 기존의 치료제들의 작용기작과는 다른 약품의 개발이 기대된다. 또한 ICAM-1은 염증반응 및 암의 전이에도 관련되어 있으므로, 동맥경화 관련 질환이외에도 다양한 질환에 대한 의약품으로의 응용도 기대된다.
호장근의 쿠마린에 의한 RAW 264.7 세포주의 nitric oxide 생성 저해활성
노태철,최희철,이승웅,김영호,노문철,김영국,이현선,Rho, Tae-Cheol,Choi, Hee-Cheol,Lee, Seung-Woong,Kim, Young-Ho,Rho, Mun-Chual,Kim, Young-Kook,Lee, Hyun-Sun 한국생약학회 2001 생약학회지 Vol.32 No.3
During the screening for inhibitors of nitric oxide production in LPS-activated macrophage, RAW 264.7 cells, two coumarins were isolated from chloroform extract of the root of Polygonum cuspidatum. They were identified as decursin (1) and decursinol angelate (2) on the basis of spectroscopic methods. The $IC_{50}$ values of these compounds on NO production were $0.76\;{\mu}M$ (1) and $2.6\;{\mu}M$ (2), respectively. However, the iNOS activity was not inhibited by treatment with these compounds. Their inhibitory effect on NO production seems to result from the suppression of iNOS induction through the suppression of NF-kB activity.
Diacylglycerol Acyltransferase에 대한 생약자원의 저해활성 검색
이승웅,고정숙,권오억,이상명,김영호,노문철,김영국,이현선,Lee, Seung-Woong,Ko, Jung-Suk,Kwon, Oh-Eok,Lee, Sang-Myung,Kim, Young-Ho,Rho, Mun-Chual,Kim, Young-Kook,Lee, Hyun-Sun 한국생약학회 2001 생약학회지 Vol.32 No.3
The inhibitory effects of methanol extracts of 135 medicinal herbs on diacylglycerol acyltransferase (DGAT) activity were investigated. DGAT was partially purified from rat liver. Eleven kinds of methanol extracts of medicinal herbs including Evodiae Fructus showed a mild inhibitory effect with the concentration of $125\;{\mu}g/ml$ (above 40% inhibition). Six kinds of methanol extracts including Ephedrae Herba exhibited a weak inhibition. Among them, three kinds of butanol extracts (Sophorae Radix, Arecae Semen, Caesalpiniae Lignum) and the chloroform extracts of Evodiae Fructus showed significant inhibitory activities (above 60% inhibition) at the same concentration.
바위수염 추출물의 파골세포 분화 억제 및 에스트라디올 활성 평가
하현주(Hyun Joo Ha),임형진(Hyung Jin Lim),김민경(Min Gyeong Kim),박선경(Seon Gyeong Bak),노문철(Mun-Chual Rho),정선희(Sun Hee Cheong),이승재(Seung-Jae Lee),이상훈(Sang-Hoon Lee) 한국해양바이오학회 2020 한국해양바이오학회지 Vol.12 No.2
In the present study, the estrogenic activity and anti-osteoclastogenic activity of the Myelophycus simplex extract were evaluated using T47D-Kbluc cells and bone marrow-derived macrophages (BMMs). As a result of the measurement of the estrogenic activity in the T47D-Kbluc cell line, the Myelophycus simplex extract showed increased estrogenic activity in a dose-dependent manner in association with its concentration. To confirm the regulatory effect of the Myelophycus simplex extract on the estrogen-responsive gene, the Myelophycus simplex extract showed a similar tendency to estradiol: the expression of estrogen receptor 1 (ESR1) was significantly decreased while the expression of estrogen receptor 2 (ESR2) was increased. Furthermore, the Myelophycus simplex extract exhibited an inhibitory effect on osteoclast differentiation. In conclusion, these Myelophycus simplex extracts might be regarded as candidates for further studies or the development of functional food products or medicine to prevent or avoid postmenopausal symptoms for women.
박선경(Seon Gyeong Bak),임형진(Hyung Jin Lim),원영선(Yeong-Seon Won),박은재(Eun Jae Park),오제훈(Je Hun Oh),김지은(Ji Eun Kim),이민지(Min Jee Lee),이소영(Soyoung Lee),이승웅(Seung Woong Lee),이승재(Seung Jae Lee),노문철(Mun Chual Rho 한국축산식품학회 2023 Food and Life Vol.2023 No.1
The skin is the largest organ that blocks invasion to the outside. Due to external stimuli, the skin barrier collapses and immune function abnormalities occur. This leads to skin diseases such as atopic dermatitis and psoriasis. Through this study, we attempted to develop a natural material that can be used for various dermatitis diseases. In this study, the gene expression of skin barrier-related proteins was confirmed using tumor necrosis factor-α/interferon-γ-stimulated HaCaT cells through real-time PCR. As a result, it was confirmed that the gene expression of skin barrier-related proteins and ceramide-related proteins was improved by the ABE-M. In this study, it was confirmed that the gene expression of filaggrin, involucrin, and loricrin, which are skin barrier-related proteins, was improved by ABE-M, and it was confirmed that the gene expression of the tight junction-related proteins, occludin and zonula occludens proteins-1, was also increased. As such, it is expected that ABE-M can be used as a material for skin inflammation such as atopic dermatitis.
부추와 마늘 바이러스의 分離 同定 및 理化學的 性質에 관한 硏究
노문철,박우원,장무웅 嶺南大學校 基礎科學硏究所 1991 基礎科學硏究 Vol.11 No.-
부추와 마늘은 바이러스 病에 대한 防止策없이 오랬동안 裁培되어온 韓國의 重要한 채소류 중의 하나이다. 따라서 이러한 바이러스 病을 防止하기 위해서는 부추와 마늘의 바이러스에 관한 基本的인 情報가 더욱 必要하다. 이러한 重要性때문에 本 硏究는 부추와 마늘의 바이러스를 分離 同定하여 그 特性을 調査하였다. 그 結果는 다음과 같다. 1. 부추와 마늘의 바이러스는 低速과 高速 遠心分離에 의해 純化되었고, 電子顯微鏡으로 觀察한 結果, 길이 650-700nm의 絲狀 粒子로 確認되었다. 2. 汁液 接種에 의해 感染된 명아주와 잠두의 잎은 局部病斑 症狀을 나타내었다. 3. 부추, 명아주, 잠두의 罹病葉을 초박절편하여 電子 顯微鏡으로 觀察한 결과, 바이러스 粒子는 集團 및 散在像으로 細胞質內에 存在하였다. 4. SDS-PAGE法으로 電氣泳動한 結果, 부추와 마늘의 바이러스의 coat protein의 分子量은 4.12×10⁴dalton으로 確認되었다. 5. 1.4% agarose gel 造成으로 水平電氣泳動한 結果, 부추와 마늘의 바이러스의 RNA의 分子量은 2.85×?? dalton으로 確認되었다. 6. GLV의 항혈청을 이용한 혈청학적 실험(면역전기영동법, 면역전자현미경법)에서 CCLV와 GLV가 동일한 반응을 나타냈었다. Chinese chive(Allium tuberosum) and garlic(Allium sativum) are an important vegetable crops for the korean people and have long been cultivated extensively in Korea. Because of this importance. studies were initiated to isolate and characterize the chinese chive latent virus(CCLV) and garlic latent virus(GLV). Result of these studies are summarized as follows: 1. CCMV and GLV were purified by alternate high and low speed centrifugation of chinese chive leaf or garlic leaf extracts. 2. Electron microscopic examination of CCLV and GLV revealed rod shaped. These paticles are measured about 650-700nm in length. 3. In manual inoculation, these viruses induced local lesion on Chenopodium album and broad bean. 4. In ultrathin section, Particles of CCLV and GLV were often found aggregate or scatered in the cytoplasm. 5. The molecular weight of the coat protein of CCLV and GLV were estimated by polyacrylamade gel electrophoresis in the presence of sodium dodecyl sulfate. Coat protein subunit of CCLV and GLV estimated about about 4.12×10⁴ daltons. 6. The moleculat weight of the nucleic acid of CCLV and GLV estimated about 2.85×?? daltons. 7. By the method of immunoelectrophoresis and immunosorbent electron microscope it has been proved that CCLV is identical to GLV.