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C57BL/6 마우스에서 기능성 샴푸 Bonogen의 양모 촉진 효과
홍진태(Jin-Tae Hong),이세라(Se-Ra Lee),김환희(Hwan Hee Kim),조영광(Young-Kwang Jo),백인정(In-Jeoung Baek),연정민(Jung-Min Yon),남상섭(Sang-Seop Nahm),곽동훈(Dong Hoon Kwack),이정은(Jung Eun Lee),이범준(Beom-Jun Lee),윤영원(Young-Won 한국독성학회 2006 Toxicological Research Vol.22 No.3
Bonogen shampoo is composed of several plant extracts which are known to be used in oriental medicine. This study was carried out to investigate the effects of Bonogen shampoo on hair growth in an alopecia model of C57BL/6 mice. There were eight male and female experimental groups including distilled water (DW; negative control), a commercial shampoo [M], 3% minoxidil (MXD) and Bonogen shampoo (BNG). Dorsal skin hair of six-week-old mice was trimmed with an electric clipper carefully not to damage the skin. The next day, mice without skin scratch were selected, randomized and separated in 10 mice per group. The test compounds were topically treated with 0.15 ml per mouse on dorsal skin for 21 days daily and then washed thoroughly with DW. The hair regrowth was determined photographically at 0, 4, 7, 10, 15, 18, and 21 days and histologically at day 21. No clinical signs were observed in all mice. Although body weight was slightly increased in 3% MXD group than other groups, it was not significant. Hair regrowth began to be promoted after 14 days and appeared a distinct regrowth pattern in all animals by topical treatment of test compounds at 18 days. In particular, the topical treatment of bonogen shampoo or 3% MXD for 21 days to dorsal skin accelerated hair regrowth faster than DW or M shampoo. At 21 days, the hair regrowth promotion speed was in order of 3% MXD > BNG > M > DW. The bonogen shampoo or 3% MXD also promoted hair follicle elongation compared to the negative control. These results suggest that bonogen shampoo has hair growth promoting activities and may be useful for treatment of bald or alopecia.
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