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생체 시료 중 아세틸콜린 및 콜린에 대한 효소-분광학적 정량분석
남명화(Myung Hwa Nam),이성호(Sung-Ho Lee),김기택(Ketack Kim),한영희(Younghee Hahn) 대한약학회 2012 약학회지 Vol.56 No.4
In order to determine acetylcholine and choline in the biological samples, the specific enzymes of acetylcholinesterase (AChE) and choline oxidase (ChO), which utilize acetylcholine and choline as substrates, were employed to convert substrates to H2O2. The produced H2O2 was coupled to 4-aminoantipyrine/phenol with peroxidase (PO) yielding quinoneimine dye which was measured at 508 nm. In the present enzymatic spectrophotometric analysis the product at the equilibrium state was measured considering accuracy, precision, time and cost of the analysis. The developed analytical method yielded good linearity (calibration curve; A508=9534[acetylcholine]+0.009, correlation coefficient (R2); 0.999) with detection limit of 1.11×10-7 M, reasonable precision (relative standard deviation; 0.10~1.62% at 2.5×10-6 M~1.25×10-4 M) and accuracy (relative error; -0.24~0.97% at 4.13×10-6M~1.01×10-4 M) for acetylcholine chloride standard solution. The concentrations of acetylcholine and choline in human serum were found as 3.20×10-5 M and 1.14×10-4 M, respectively. The brain tissues of Sprague-Dawley strain rat contained 9.82 μg/g of acetylcholine and 6.53 μg/g of choline in the cerebrum, while 7.37 μg/g of acetylcholine and 5.34 μg/g of choline in the cerebellum.
BaSn(BO<sub>3</sub>)<sub>2</sub>세라믹스의 저온소결 및 유전특성
남명화,김효태,황준철,남중희,여동훈,김종희,남산,Nam, Myung-Hwa,Kim, Hyo-Tae,Hwang, June-Cheol,Nam, Joong-Hee,Yeo, Dong-Hoon,Kim, Jong-Hee,Nahm, Sahn 한국세라믹학회 2006 한국세라믹학회지 Vol.43 No.2
Dolomite type $BaSn(BO_3)_2$ ceramics with rhombohedral crystal structure has been synthesized via solid state reaction route. Dielectric properties were measured for the samples sintered at $1050\~1200^{\circ}C$ for 2 h in air. Dielectric constant, loss tangent, and temperature coefficient were increased with sintering temperature due to the evolution of $BaSnO_3$, secondary, phase. Optimum dielectric properties were obtained at the $BaSn(BO_3)_2$ ceramics sintered at $1100^{\circ}C.\;CuO/Bi_2O_3$ was added to $BaSn(BO_3)_2$ ceramics to lower the sintering temperature for LTCC application, then Co and Fe-based coloring agents were added for colorizing the LTCC tape. Typical dielectric properties of $BaSn(BO_3)_2$ ceramics with $5 wt\%\;CuO/Bi_2O_3\;and\;3wt\%$ Co-coloring agent that sintered at $900^{\circ}C$ were $\varepsilon_r=9.89,\;tan{\delta}=0.92\times10^{-3},\;and\;TCC=112ppm/^{\circ}C$. Thus obtained LTCC tape was co-fired with Ag paste for compatibility test and revealed no sign of Ag reaction with the ceramics.
CaZr(BO<sub>3</sub>)<sub>2</sub> 세라믹스의 마이크로웨이브 유전특성
남명화,김효태,김종희,남산,Nam, Myung-Hwa,Kim, Hyo-Tae,Kim, Jong-Hee,Nahm, Sahn 한국세라믹학회 2007 한국세라믹학회지 Vol.44 No.5
The microstructure and microwave dielectric properties of dolomite type borates, $CaZr(BO_3)_2$ ceramics prepared by conventional mixed oxide method were explored. The sintering temperature of $CaZr(BO_3)_2$ ceramics could be reduced from $1150^{\circ}C\;to\;925^{\circ}C$ with little amount of sintering additives. Microwave dielectric properties of 3 wt% $Bi_2O_3-CuO$ added $CaZr(BO_3)_2$ ceramics sintered at $925^{\circ}C$ were $K{\approx}10.4,\;Q{\times}f{\approx}80,000GHz\;and\;TCF{\approx}+2ppm/^{\circ}C$. Thus obtained LTCC tape was co-fired with Ag paste for compatibility test and revealed no sign of Ag reaction with the ceramics. Therefore, $CaZr(BO_3)_2$ ceramics is considered as a possible candidate material for low temperature co-fired multilayer devices.
의료관광 유치기관 종사자들의 심리적 자본과 조직몰입 간 관계에서 내재적동기의 매개효과
인성호(Sung Ho In),남명화(Myung Hwa Nam),정경일(Kyung Il Chung) 한국관광연구학회 2014 관광연구저널 Vol.28 No.7
The purpose of this study is to investigate the mediating effects of intrinsic job motivation on the relationship between the psychological capital and organizational commitment in the medical tourism corporations. Data were collected from medical tourism corporations such as hospitals and clinics which were located in Seoul. A total of 348 surveys were distributed and 297 were analyzed. To summarize the results of the study; first, psychological capital was revealed to have a positive effect on intrinsic job motivation and organizational commitment. Second, intrinsic job motivation has a positive effect on organizational commitment of employees. Third, Intrinsic job motivation was mediated in the relationship between the psychological capital and organizational commitment. The results of the study will suggest a specific guideline for establishing human resource management policies in the medical tourism corporations. Also, It will be helpful for hospital and government officials to develop a future management framework for the medical tourism industry.
여행사의 맥락에서 고객 지향 및 서비스복구노력에서의 감성 지능의 효과
인성호 ( Sung-ho In ),김정훈 ( Jung-hoon Kim ),남명화 ( Myung-hwa Nam ) 대한관광경영학회 2016 觀光硏究 Vol.31 No.4
본 연구의 목적은 서비스실패에 따른 종사원들의 서비스회복 노력에 대한 필요성으로 서비스 제공자인 여행사 종사원의 개인역량으로 감성지능과 서비스회복 능력의 영향력을 살펴봄으로서, 종사원의 고객지향성이 종사원의 감성지능과 서비스회복 노력의 관계에 고객지향성의 매개역할을 검증하고자 하였다. 이를 위해 우리나라를 대표하는 여행사 중 모두투어, 하나여행사, 자유투어, 롯데관광에 근무하는 종사원들을 표본대상으로 선정하여, 2014년 11월 11일∼12월 10일에 걸쳐 설문조사를 실시하였다. 설문조사 결과 총 380부를 배부하여, 이중에서 불성실하게 응답했거나 연구목적에 적합하지 않다고 판단되는 설문지를 제외한 총310(81.6%)매를 분석에 사용하였다. 연구의 결과는 먼저, 감성지능의 감성활용, 감성조절, 타인감성이해, 자기감성이해 고객지향성에 정(+)의 영향을 미치는 것으로 나타났고, 고객지향성은 서비스회복노력에 정(+)의 영향을 미치는 것으로 나타났으며, 고객지향성의 매개효과 결과를 해석해 보면, 감성지능과 서비스회복노력 간의 관계에 있어, 고객지향성은 부분매개하고 있는 것으로 나타났다. 본 연구의 결과를 통해 여행사에 근무하는 종사원의 감성지능이 고객지향성 및 서비스회복노력에 긍정적인 영향을 미치는 점을 실무적 측면에서 논의하였고, 고객지향성이 감성지능과 서비스회복노력의 관계를 조절한다는 시사점을 제시하였다. 또한, 일부 연구의 한계점과 향후 연구방향을 제시하였다. This study examines the effects of emotional intelligence on service recovery efforts based on the capability of employees as service providers in travel agencies. The study also verifies the mediating role of customer orientation in the relationship between emotional intelligence and service recovery efforts. For this, the employees of travel agencies in Korea, were considered for the sample, and a field survey was conducted November 11 to December 10, 2014. A total of 380 individuals were provided with the questionnaire, and with those responses with missing data excluded, a total of 310 responses were obtained for the analysis. According to the results, the four dimensions of emotional intelligence, namely applying emotions, controlling emotions, understanding others’ emotions, and understanding one’s own emotions, had positive effects on customer orientation, which in turn had a positive effect on service recovery efforts. Customer orientation mediated the relationship between emotional intelligence and service recovery efforts. The results indicate that travel agency employees`` emotional intelligence had considerable influence on their customer orientation and service recovery efforts in a practical manner. In addition, customer orientation moderated the relationship between emotional intelligence and service recovery efforts. Some limitations and avenues for future research are discussed.
南明和,丁海崙 고려대학교 의과대학 1986 고려대 의대 잡지 Vol.23 No.1
The patients with various ocular diseases frequently rub their eyes. The author studied the effects of eye rubbing on the histologic characteristics cf the conjunctiva. Each one eye of twenty albino rabbits were rubbed on the upper lid with finger for five-minute period, and the unrubbed contralateral eye served as a control. The upper tarsal conjunctivas were taken immediately, at four. eight, and twenty-four hours after the rubbing. After fixation of specimens in Kernovsky's fixative, the tissues were processed for preparation of Epon block and one-micron section. Sections were stained with alkaline Giemsa and toluidine blue arid light microscopic examination was performed. The results were as followings : Immediately after rubbing, the conjunctival epithelium was disrupted and the mast cells became degranulation. At four hours, there was moderate infiltration of neutrophils. At eight arid twenty-four hours after rubbing, infiltration of macrophages was occurred and increased in cumber. But there was no significant changes in numbers of lymphocytes, plasma cells, and eosinophils. This study revealed that eye rubbing induces conjunctival erythema and alteration in the inflammatory cell infiltrate, and therefore eye rubbing map influence the course and severity of ocular diseases.