http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
김희선,설혜원,안희진,오선경,구승엽,김석현,최영민,김정구,문신용,Kim, Hee-Sun,Seol, Hye-Won,Ahn, Hee-Jin,Oh, Sun-Kyung,Ku, Seung-Yup,Kim, Seok-Hyun,Choi, Young-Min,Kim, Jung-Gu,Moon, Shin-Yong 대한생식의학회 2004 Clinical and Experimental Reproductive Medicine Vol.31 No.4
Objective: This study was performed to evaluate the possibility of prolonged culture of human embryonic stem cells (hESC; SNUhES2) on human amniotic fluid cells (hAFC), which had been storaged after karyotyping. Method: The hAFC was prepared for feeder layer in the presence of Chang's medium and STO medium (90% DMEM, 10% FBS) at $37^{circ}C$ in a 5% $CO_2$ in air atmosphere. Prior to use as a feeder layer, hAFC was mitotically inactivated by mitomycin C. The hESCs on hAFC were passaged mechanically every seven days with ES culture medium (80% DMEM/F12, 20% SR, bFGF). Results: The hAFC feeder layer support the growth of undifferentiated state of SNUhES2 for at least 59 passages thus far. SNUhES2 colonies on hAFC feeder appeared slightly angular and flatter shape as compared with circular and thicker colonies observed with STO feeder layer and showed higher level with complete undifferentiation in seven days. Like hESC cultured on STO feeders, SNUhES2 grown on hAFC expressed normal karyotype, positive for alkaline phosphatase activity, high telomerase activity, Oct-4, SSEA-3, SSEA-4, Tra-1-60 and Tra-1-81 and formed embryoid bodies (EBs). Conclusion: The hAFC supports undifferentiated growth of hESC. Therefore, these results may help to provide a clinically practicable method for expansion of hESC for cell therapies.
동결보존시 생쥐 전핵배아의 시기에 따른 생존율과 발생율의 비교
김희선,류범용,오선경,서창석,김석현,최영민,김정구,문신용,이진용,Kim, H.S.,Ryu, B.Y.,Oh, S.K.,Suh, C.S.,Kim, S.H.,Choi, Y.M.,Kim, J.G.,Moon, S.Y.,Lee, J.Y. 대한생식의학회 1998 Clinical and Experimental Reproductive Medicine Vol.25 No.1
This study was designed to evaluate the influence of pronuclear age on the survival and post-thawing development after cryopreservation of mouse embryos. Freezing and thawing were performed in the different pronuclear stages of mouse embryos after IVF. Embryos were obtained from $F_1$ hybrid mice and classified into 4 groups according to the pronuclear stage (6hr, 9hr, 12hr and 15hr after insemination). Pronuclear ova were slowly cooled in a biological freezer using 1.5M 1,2-propanediol and 0.1M sucrose as cryoprotectant. Thawing was done at room temperature and 1,2-propanediol was removed by multi-step dilutions. Both frozen-thawed embryos and control fresh embryos were cultured in vitro in Ham's F-10 medium supplemented with 4mg/ml BSA. In control group, the development rate after 48hr was 99.3%, and the complete hatching rate after 144hr was 61.3%. In experimental groups, the survival rate after thawing was 95.4% in 6hr, 88.7% in 9hr, 75.2% in 12hr and 62.4% in 15hr after insemination, the development rate after 48hr was 61.1, 77.0, 67.0 and 79.6%, respectively, and the complete hatching rate after 144hr was 25.7, 43.7, 42.2 and 60.0%, respectively. The survival rate in 15hr was significantly lower (p<0.05) compared with other groups. In vitro development rates after 48hr were similar in all groups, but complement hatching rate was significantly lower (p<0.05) in 6hr group. In conclusion, cryopreservation of mouse pronuclear ova with 2 distinct pronuclei (9hr and 12hr groups) showed better results after thawing compared with early (6hr group) or late pronuclear ova just prior to cleavage (15hr group).
국내 야생따쥐(Crocidura lasiura) 의 Hantavirus 항체 보유실태 및 원인체 분리
김희선,강문일,Kim, Hee-sun,Kang, Mun-il 대한수의학회 1994 大韓獸醫學會誌 Vol.34 No.1
Eleven shrews were caught from three areas of Korea. All of them were confirmed in the same species, Crocidura lasiura. All of sera from wild shrews were examined by indirect immunofluorescent test against Hantaan-related virus. The antibody to Hantaan-related virus was detected by 2 out of 11 shrews. Just 2 of 7 shrews from BG area were sero-positive for Hantaan-related virus antigen and none from other. All of sero-positive for Hantaan-related virus antigen belonged to male with antibody titer of 1:40 to 1:80. Two Hantaan-related viruses were isolated in vivo and in vitro.
국내 야생 집쥐(Rattus norvigicus)의 유행성 출혈열바이러스 감염증의 역학조사
김희선,이준학,강문일,고홍범,Kim, Hee-sun,Graham, Robert Ross,Lee, Jun-hak,Kang, Mun-il,Koh, Hong-bum 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.2
Sixty eight wild rats were caught from Seoul, Kyonggi, Kangwon, Cholla, and Kyongsang provinces in Korea. All of them were confirmed in the same species, Rattus norvigicus. All of sera from wild rats were examined by indirect immunofluorescent test against Hantaan-related virus. Detection of anti-body to Hantaan-related virus were 4 out of 68 rats(5.9%), including 2 of 8(25.0%) rats in Kangwon and 2 of 20 rats(10.0%) in Kyongsang province. No difference of the sero-positive rates between sex of rats tested to Hantaan-related virus was recognized. According to age, four rats with antibodies(1:320~640) to Hantaan-related virus belonged one subadult, one young adult, one middle aged adult, and one old adult, respectively.
국내 야생 집쥐(Rattus norvegicus)의 murine typhus 감염조사
김희선,강문일,고홍범,Kim, Hee-sun,Graham, Robert Ross,Kang, Mun-il,Koh, Hong-bum 대한수의학회 1994 大韓獸醫學會誌 Vol.34 No.4
Sixty eight wild rats, Rattus norvegicus, were caught from Seoul city, Kyonggi, Kangwon, Cholla, and Kyongsang-provinces. All sera collected from rats were examined by immunofluorescent test to find antibody against murine typhus. The antibody prevalance to murine typhus was 4.4%(3/68). Sero-positive rate was 12.5%(2/16) in Kyonggi province and 12.5%(1/8) in Cholla province. The sero-positive rate difference between sexes of rats was not recognized. Three Rattus norvegicus having antibody to murine typhus were morphologically classified as subadult with 8.3%(1/12), middle-aged adult with 5.9%(1/17), and old adult with 4.8%(1/21), respectively.
의사결정나무 분석을 이용한 이상지질혈증 유병자의 지질관리 취약군 예측: 2019-2021년도 국민건강영양조사 자료
김희선,정석희,Hee Sun Kim,Seok Hee Jeong 한국기초간호학회 2023 Journal of korean biological nursing science Vol.25 No.2
Purpose: The aim of this study was to assess lipid levels and to identify groups with poor lipid control group among patients with dyslipidemia. Methods: Data from 1,399 Korean patients with dyslipidemia older than 20 years were extracted from the Korea National Health and Nutrition Examination Survey. Complex sample analysis and decision-tree analysis were conducted with using SPSS for Windows version 27.0. Results: The mean levels of total cholesterol (TC), triglyceride (TG), low density lipoprotein-cholesterol (LDL-C), and high density lipoprotein cholesterol were 211.38±1.15 mg/dL, 306.61±1.15 mg/dL, 118.48±1.08 mg/dL, and 42.39±1.15 mg/dL, respectively. About 61% of participants showed abnormal lipid control. Poor glycemic control groups (TC ≥ 200 mg/dL or TG ≥ 150 mg/dL or LDL-C ≥ 130 mg/dL) were identified through seven different pathways via decision-tree analysis. Poor lipid control groups were categorized based on patients' characteristics such as gender, age, education, dyslipidemia medication adherence, perception of dyslipidemia, diagnosis of myocardial infarction or angina, diabetes mellitus, perceived health status, relative hand grip strength, hemoglobin A1c, aerobic exercise per week, and walking days per week. Dyslipidemia medication adherence was the most significant predictor of poor lipid control. Conclusion: The findings demonstrated characteristics that are predictive of poor lipid control and can be used to detect poor lipid control in patients with dyslipidemia.
김희선,여인권,Kim, Hee-Sun,Yeo, In-Kwon 한국통계학회 2011 응용통계연구 Vol.24 No.6
주가연계증권(ELS)은 주가와 연동되어 수익률이 결정되는 금융상품으로 현재 증권회사뿐만 아니라 은행에서도 많이 판매되고 있는 간접투자상품이다. 이 논문에서는 수익다이어그램을 통해 대표적인 주가연계증권의 수익구조를 알아보며 특히 현재 가장 많이 판매되고 있는 원금비보장 상품인 스텝다운형에 관심을 가진다. KOSPI 200과 홍콩 HSI를 기초자산으로 설정한 스텝다운형 상품에서 투자시점의 시계열 특성에 따른 조기상환여부와 경험적 분포를 이용한 VaR를 통한 위험률에 대해 고찰해 본다. Equity linked securities are indirect investments where the return of investment depends on the performance of the underlying equities. In this paper, we review the profit structure of typical equity linked securities through a profit diagram and investigate which characteristics of time series at the investment affect the early repayment of the stepdown ELS based on KOSPI 200 and HSI. We also compare VaRs using the empirical distribution function for risk management.