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Potential Applications of Recombinant DNA Probes for Relatedness Analysis of Fusarium axysporum
Kim, Hong Gi,Kim, Young Tae,Yu, Seung Hun 충남대학교 생물공학연구소 1996 생물공학연구지 Vol.4 No.-
Randomly chosen recombinant clones of Fusarium oxysporum were analysed to select useful probes for relatedness analysis of Fusarium oxysporum. Genomic DNA of F. oxysproum f. sp. cubense, digested with HindⅢ, was ligated to pUC118 and used to transform Escherichia coli strain DH5a. Three clones were identified that hybridized to multiple restriction fragments of some formae speciales of F. oxysporum. These probes detected repetitive sequences in HindⅢ or EcoRI digested DNAs. Repeated copy clone pFC46, pFC52 and pFC54 showed evident polymorphisms among ten formae speciales of theis fungus. Since clone pFC 52 strongly hybridized to multiple EcoRI-digested restriction fragments of f. sp. cubense, it may be useful as a probe for analysis of other genetic characteristics of this forma specialis. The results suggest that our clones might be very useful as probes for relatedness analysis between or within formae speciales of Fusarium oxysporum.