성격 특성에 관한 체질판별 문진항목의 통계적 분석

김흥기,김종열,Kim, Hong-Gie,Kim, Jong-Yeol 사상체질의학회 2003 사상체질의학회지 Vol.15 No.3

Objectives : To evaluate the properness and effectiveness of the question categories concerning 'sasang constitutional characteristics of mind', used in Iksan Wonkwang Oriental Medicine. Methods : We statistically analyzed data from those 1335 patients focussing on 'relative discrimination ability' to sasang constructions and 'response ratio'. Patients included in this research are only those who had been well treated in Iksan Wonkwang Oriental Medicine during the Period of three years from 2000 to 2002. The data are obtained through the electronic chart developed by Kim Jong-Yeal, and analyzed using the statistical Package SPSS. Results: In total characteristics of mind, the question category of 'timid' has high discriminating power and effectiveness to Soeum type, more in women than men, and especially with an age of 21 or more; The category of 'hasty-tempered' to Soyang type in women with all ages and men with ages 21-40; The category of 'broad-minded' to Taeum type with ages 41 or more; The category of 'timid and broad-minded' to Soyang type, and especially high to women with ages 41 or more. Question categories concerning response type in anger were estimated to have different response patterns to sasang constructions only for ages 21 or more by $X^2$2 test. The question category of 'bear and last long' has high discriminating power and effectiveness to Soeum type, more in men than women, and increasingly to ages; The category of 'impatient and disarmed quickly' to Soyang type in both women and men, especially with ages 21 or more; The category of 'impatient' to Soyang type women or with ages 21 or more. Other categories have low discriminating power or low effectiveness. Concernig expression type of one's thought, the question category of 'straightly' has high discriminating power and effectiveness to Soyang type, more in wemen than men, and especially to ages 21 or more; The category of 'tender' to Soeum type, in women or with ages 40 or less; The category of 'indirectly' to Taeum type in men or with ages 41 or more; The category of 'straightly and stuborn' to Soyang type with ages 41 or more; The category of 'indirectly and tender' to Soeum with with ages 20 or less; The category of 'not express and tender' to Soeum type with ages 41 or more; Conclusions : Question categories concerning total characteristics of mind, response type in anger and expression type of one's thought, are partially discriminating and effective to specific constitution according to sex and ages, and some categories are more discriminating and effective when they are composited. But generally, the discriminating power of Taeum constitution group and group with ages 20 or less was too low, so it is necessary to develope question categories for those groups.

세계최대규모의 시화호 조력발전사업

김흥기,백두현,Kim, Heung-Gi,Bae, Doo-Hyun 한국유체기계학회 2003 한국유체기계학회 논문집 Vol.6 No.2

재활용 목표율 67% 상향

김흥기,Kim, Heung-Gi (사)한국포장협회 2006 包裝界 Vol.153 No.-

Zoom in: 개인정보보호법 시행, 그 의미와 대응

김흥기,Kim, Heung-Gi 한국발명진흥회 2011 發明特許 Vol.36 No.9

SAW 여기를 위한 EMAT의 설계 및 제작에 관한 연구

김흥기,이재승,전계석,Kim, Heung-Ki,Lee, Jae-Seung,Jun, Kye-Suk 한국음향학회 1990 韓國音響學會誌 Vol.9 No.3

In this paper, meander line type EMAT(Electro-Magnetic Acoustic Transducer) has been designed and fabricated with effective properties for detecting flaw existing within one wavelength in depth, and its characteristics have been analyzed. For the purpose of getting effective dynamic and static magnetic intensity, the coil has been arrayed using wire with interval of 0.75 mm and width of 0.65 mm and permanent magnets with 1500 Gauss have been constructed respectively. The center frequency and fractional bandwidth of the fabricated EMAT was 2 MHz and 36% respectively and its impulse response has been measured by non-contacting technique(the distance between the conducting media and the coil was 0.15mm). In the measuring results, it has been shown that Insertion Loss(IL) was 45.46dB and it was good agreement with theoretical result. 본 연구에서는 표면에서 한파장 깊이 이내에 존재하는 결함 검출에 우수한 특성을 나타내는 meander line형 EMAT를 설계, 제작하고 그 특성을 연구분석하였다. 효율적인 동 자기장을 위하여 간격 0.72mm와 폭 0.65mm의 코일을 구성하였으며 정 자기장을 위하여 1500 가우스의 자석군을 구성하였다. 제작된 EMAT는 중심 주파수 2MHz, 비대역폭 36%를 가지며 임펄스응답 특성은 0.15 mm의 비접촉 방법으로 측정되어 이론과 비교되었고 중심 주파수에서의 삽입손실은 45.46 dB로 측정되었다.

재해·재난 극복을 위한 임시주택 제안

김흥기,Kim, Heunggee 대한건축사협회 2019 建築士 Vol.602 No.-

회원작품 - 강릉 메어블릭

김흥기,Kim, Heunggee 대한건축사협회 2018 建築士 Vol.586 No.-

회원작품 - 올림픽공원 옆 가게 _ 경량철골 근생-K1

김흥기,Kim, Heunggee 대한건축사협회 2019 建築士 Vol.601 No.-

구창 인후염 완선

김흥기,Kim, Heung-Gi 대한한약협회 2001 大韓漢藥 Vol.5 No.-

Cisplatin 내성 난소암 세포주 Clone의 DNA Mismatch Repair 유전자 변이및 Taxol, Topotecan의 민감도

김흥기(Heung Ki Kim),손우석(Woo Seok Son),박태철(Tae Chul Park),김태응(Tae Eung Kim),고영미(Young Me Koh),송지민(Ji Min Song),박기영(Ki Young Park),김재훈(Jae Hoon Kim),유기성(Ki Seong Ryu),김진우(Jin Woo Kim),남궁성은(Sung Eun NamKoo 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.11

목적: 부인 종양에서 가장 심각한 문제중 하나인 난소암은 항암화학요법에 비교적 민감하게 반응하는 고형 종양이나 다양한 노력에도 불구하고 사망률은 큰 변화가 없는 실정이다. Cisplatin은 이들 항암제의 중심이며 가장 효과적인 약제이지만, Cisplatin에 대한 약제내성의 출현은 치료 실패의 중요한 원인이 되고 있고, 최근 Hereditary nonpolyposis colorectal cancer에서 입증된 DNA 부정합 보수 유전자의 변이가 여러 약제에 대하여 약제내성을 초래한다는 사실이 밝혀지고 있다. 이에 따라 Cisplatin 내성을 갖는 난소암 세포주에서 DNA 부정합 보수 유전자인 hMLH1과 hMSH2의 돌연변이와 Microsatellite instability 여부를 알아보고, 약제내성을 갖는 난소암 세포주에서 얻은 단일 세포의 clone들중 부정합 보수 유전자에 변이가 있는 clone과 정상인 clone을 분류하여, 이들에서 난소암의 2차 약제로 많이 쓰이는 Taxol, Topotecan의 항암제 민감도에 차이가 있는지 밝히고자 연구를 시행하였다. 연구방법: 상피성 난소암 세포주 2008과 이 세포주에 Cisplatin의 농도를 반복적으로 증가시켜 만들어진 2008/C13*5.25 세포주에서 추출한 cell clone으로 hMLH1과 hMSH2 단백질로 immunoblot을 시행하고 각각의 clone에 대하여 taxol 및 topotecan의 colony forming assay를 이용하여 항암제 민감도 검사를 시행하였다. 결과: 1. 2008 세포주와 2008/C13*5.25 세포주에서 hMLH1과 hMSH2에 대한 항체를 이용하여 immunoblot을 시행한 결과 각각의 세포주에서 모두 hMLH1과 hMSH2 단백질의 발현을 확인 할 수 있었다. 2. 2008 세포주에서 hMLH1(+)인 clone은 78개, hMLH1(-)인 clone은 22개 였고, 2008/C13*5.25 세포주에서는 hMLH1(+)인 clone은 73개, hMLH1(-)인 clone은 27개로, hMLH1(-)로의 변이는 2008/C13*5.25에서 약간 많았지만 통계적으로 유의한 차이는 없었다. 3. 2008과 2008/C13*5.25 세포주에서는 Taxol에 대한 민감도에 차이가 없었다. 그러나 2008/C13*5.25 hMLH1(-)의 clone들이 hMLH1(+)인 경우 보다 유의하게 Taxol에 민감하였고(P=0.049), 2008 hMLH1(+)인 clone들은 hMLH1(-) clone보다 유의하게 Taxol에 민감하였다(P=0.003). 4. 2008과 2008/C13*5.25 세포주에서는 Topotecan에 대한 민감도에 차이가 없었고. 2008/C13*5.25 hMLH1(-)의 clone들이 hMLH1(+)보다 유의하지는 않았지만 Topotecan에 약간 민감하였고, 2008 세포주에서는 hMLH1(-) clone들이 hMLH1(+)보다 유의하게 Topotecan에 민감하였다(P=0.001). 또한 2008과 2008/C13*5.25 두 세포주에서 hMLH1(-)인 clone들은 cisplatin 내성에 관계없이 유의하게 Topotecan에 민감하였다(P=0.001). 5. BAT25, BAT26, BAT34 primer에 대한 2008 hMLH1(+), hMLH1(-) clone과 2008/C13*5.25 hMLH1(+), hMLH1(-) clone의 microsatellite instability 결과는 모두 정상의 allele 소견으로 microsatellite instability를 나타내지 않았다. 결론: 이상의 연구 결과로 미루어 난소암에서 DNA 부정합 보수 유전자의 변이와 Cisplatin의 약제내성 사이에는 뚜렷한 관련이 없어 보이고, 난소암에서 hMLH1의 소실이 있는 경우는 오히려 Taxol이나 Topotecan에 민감한 것으로 나타났으며, 이러한 결과는 난소암 치료에서 1차 및 2차 약제의 선택에 도움을 줄 것으로 사료된다. Objective: Ovarian cancer represents a relatively chemosensitive solid tumor, with responsiveness to a range of agents. Cisplatin is the mainstay of drug treatment and is one of the most active single agent. However, the overall outcome for patients remains unsatisfactory and the emergence of drug resistance is a major factor in treatment failure. Loss of DNA mismatch repair is a common finding in many types of sporadic cancer as well as in patients with hereditary nonpolyposis colon cancer. Cells that lack DNA mismatch repair are resistant to commonly used chemotherapeutic agents. Selection of cells for resistance to cisplatin, a well-recognized mutagen, could result in mutation in genes involved in DNA mismatch repair. Methods: This study evaluated the mutation of hMLH1 and hMSH2, and its relation to the Taxol and Topotecan chemosensitivity in the clones from the ovarian cancer cell line 2008 and cisplatin-resistant cell line 2008/ C13*5.25. Results: 1. Cells from 2008 and 2008/C13*5.25 expressed both hMLH1 and hMSH2 when analysed with immunoblotting. 2. Twenty two out of 100 single-cell clones from 2008 and 27 of clones from 2008/C13*5.25 expressed no hMLH1. hMSH2 was expressed in all clones. 3. There was no difference of Taxol chemosensitivity between 2008 and 2008/C13*5.25 cell lines. In the 2008/C13*5.25 cell line, the hMLH1-deficient clones were more sensitive to Taxol than the hMLH1-proficient clones(P=0.049), but in 2008 cell lines hMLH1-proficient clones were more sesitive to Taxol(P=0.003). 4. There was no difference in Topotecan chemosensitivity between 2008 and 2008/C13*5.25 cell lines. In the 2008/C13*5.25 cell line, the hMLH1- deficient clones were not more sensitive to Topotecan than the hMLH1-proficient clones. In the 2008 cell lines hMLH1-deficient clones were more sesitive to Topotecan(P=0.001). Overall, hMLH1-deficient clones from both 2008 and 2008/C13*5.25 cell lines were significantly more sensitive to Topotecan(P=0.001). 5. Microsatellite instability was not demonstrated in all 4 types of single-cell clones from 2008 and 2008/C13*5.25 cell lines. Conclusions: The present results indicate that there is no relation between mutation of mismatch repair gene and cisplatin resistance. But hMLH1-deficient ovarian cancer cells are more sensitive to Taxol or Topotecan in this study. The latter finding mandates the examination to assess the mutation of hMLH1 in tumor cells before treatment or at the time clinical resistance to cisplatin develops in ovarian cancer.