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임현술,정해관,김정순,오희철,이동모,김호훈,Lim, Hyun-Sul,Cheong, Hae-Kwan,Kim, Joung-Soon,Ohr, Hee-Choul,Rhie, Dong-Mo,Kim, Ho-Hoon 대한예방의학회 1994 Journal of Preventive Medicine and Public Health Vol.27 No.4
This epidemiologic study was carried out to investigate cauuse and magnitude of food-poisoning like epidemic occurred among inhabitants of a village who have eaten dead cow's meat near Kyongju in February of 1994, around lunar new year, The investigation consisted of interview survey on all inhabitants of 77 households (111 males and 119 females) and their visitors (40 males and 35 females), skin test with anthraxinum (Russian product), study on clinical characteristics for the patients hospitalized, and microbiologic examination on microbes isolated from cow's meat, patient and soils of dead cow's barn. The results obtained are as followings; 1. The proportion of the inhabitants who ingested the dead cow's meat was 36.4%. The incidence rate of the disease was 65.1% for males, 41.7% for females and the cases were distributed evenly for all age groups. The group ingested raw meat showed higher incidence than the group ingested cooked meat. There was no case among people who did not eat the meat. 2. The most clinical symptoms were significantly more frequent among cases than non-cases : sore throat (57%), nausea (51%), fever (47%), indigestion (43%), cough (41%), anorexia (41%), abdominal distention (41%), and abdominal pain (39%) were the major symptoms among cases. 3. Among 29 cases hospitalized out of total 61 cases, three patients, all old and feeble persons, deceased from the disease resulting in 4.9% fatality rate among total patient and 10.3% among hospitalized. Septicemia and meningitis were the causes of the deaths. 4. Three strains isolated from patients, and three strains from dead cow's meat and soil revealed typical microbiologic characteristics of Bacillus Anthracis, which also proved to be fatal to experimentally infected mice.
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1996년 부산시에서 집단발생한 장티푸스 환자들로부터 분리한 Salmonella typhi의 PFGE양상, Phage형 및 항균제감수성
박미선,강연호,유정식,신영학,이복권,김호훈 대한감염학회 1997 감염 Vol.29 No.3
목적: 1996년 4월부터 6월사이 부산시 수영구에서 집단 발생한 장티푸스 환자로부터 분리된 장티푸스균 48주를 대상으로 phage형 시험 및 염색체 DNA를 제한효소로 처리 후 PFGE를 시행, 그 양상을 분석, 역학적 도구로서의 유용성을 검토하고 장티푸스 치료의 효율적 약제선택의 기초 자료로 제공하고자 항생제 감수성시험을 하였다. 방법: 항생제 감수성 시험은 장내세균 감수성 시험에 권장되고 있는 ampicillin외 14종의 항균제에 대하여 NCCLS 표준 디스크 확산법으로 수행하였다. phage형 시험은 영국 중앙 공중 보건 실험실(PHRL)에서 분양 받은 phage액을 사용하여 시험하였다. PFGE 시험은 장티푸스균 염색체 DNA를 제한효소 Spe Ⅰ으로 처리후 PFGE를 수행, 그 영동상을 관찰 분석하였다. 결과: 시험대상 장티푸스균 48주중 ampicillin의 14종의 항균제에 내성을 보인 균주는 없었다. phage형은 비분류주(untypable)가 44주였고 M1이 2주, 그리고 E1형이 2주였다. PFGE양상 분석결과 A group(47주) 및 B group(1주)의 2가지 group으로 분류되었으며 A group은 A1(42주), A2(1주), A3(3주) 및 A4(1주) 등 4가지 subgroup으로 분류되었다. A group의 각 subgroup사이의 유전적 유사도(F치)는 0.8-0.897으로 유사도가 높았으나 A group의 각 subgroup과 B group간의 F치는 0.20-0.258로 유전적 근연 관계가 낮았다. 결론: 1996년 4월부터 6월사이 부산시 수영구에서 집단 발생한 장티푸스 초발 환자는 장티푸스균이 분리된 환자를 대상으로 장티푸스균의 PFGE양상 분석 결과 발병일(1996년 4월 23일)이 가장 빠른 것으로 신고된 남성 환자로 추정되었다. 염색체 DNA를 Spe Ⅰ제한효소로 처리 PFGE를 수행, 그 양상을 분석하는 방법이 장티푸스 집단발생시 감염원 및 유행전파 경로 추적등 역학적 분석에 유용성이 큰 것으로 밝혀졌다. 본 실험에 사용된 장티푸스 분리균은 장내세균 약제 감수성 시험에 일반적으로 권장되고 있는 ampicillin외 14종의 항균제에 내성을 나타낸 균주는 검출되지 않았으나 항균제 남오용 및 해외로부터 약제 내성 장티푸스균의 유입으로 약제 내성 장티푸스 출현 감시에 능동적 대처가 요망된다. Background: During the period from April to June 1996, an outbreak of typhoid fever occurred in SuYoung-Ku, Pusan city. We performed the molecular epidemiological analysis and drug susceptibility test for 48 isolates of Salmonella typhi from the outbreak. Methods: Chromosomal DNA of S. typhi was digested with the restriction endonuclease Spe Ⅰ and the resulting restriction polymorphism was observed on pulsed field gel electrophoresis(PFGE). Phage typing was performed using Vi-phages, and antimicrobial susceptibility test was done by Disc diffusion method. Results: On phage typing, 2 isolates were M1 phage type, 2 E1 phage type and 44 untypable. The PFGE analysis on 14 to 16 chromosomal DNA restriction fragments ranged from 50Kb to 1000kb showed 2 different patterns of restriction fragments, divided into group A and B. Forty-seven isolates belonged to group A that were subgrouped into A1(42 strains), A2(1 strain), A3(3 strains), A4(1strain), and one strain was grouped into group B. in the F-value of genetic similarity of the epidemic S. typhi strains, there was low similarity(F value: 0.2-0.258) between group A strains and group B strains. However, the subgroup strains(A1-A4) showed high similarity(F value:0.8-0.897) each other. The drug susceptibility test showed susceptibility to ampicillin, carbenicillin, cephalothin, chloramphenicol, tobramycin, gentamycin, kanamycin, nalidixic acid, neomycin, polymyxin B, streptomycin, tetracycline, trime-thoprim-sulfamethoxazole, ciprofloxacin and norfloxacin. Conclusion: This study showed that phage typing and PFGE were very useful as a tool to investigate molecular analyses of epidemic S. typhi strains this study.
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Vibrio parahaemolyticus의 전균체 및 편모항원에 대한 항혈청의 민감성과 특이성에 관한 연구
朴抱鉉,宋哲鏞,金鎬薰 中央大學校 自然科學硏究所 1988 自然科學硏究所 論文集 Vol.2 No.-
This study was undertaken to examine the sensitvity and specificity of antisera against whole cell antigen (WCA) and flagella antigen (FA) of Vibrio parahaemolyticus. Antiserum against WCA of V. parahaemolyticus showed 8-fold high agglutination titre with homologous H-antigen (1:25,600), while low cross-reactions were obesrved with H-antigen of W. alginolyicus (1:3,200), V. furnissii (1:200) and V. vulnificus (1:100). The antiWCA serum showed not only 2-fold agglutination titre with homologous O-antigen (1:320), but also showed high cross-reactions were observed with V. holisae (1:160), V. vulnificus (1:10), V. alginolyticus (1:10) and V. cholerae (1:10). The anti-WCA serum of V. aprahaemolyticus which absorbed with homologen O-antigen and heterologous H-antigen of V. alhinolyticus showed 32-fold high agglutination titre with homologous H-antigen (1:L 12,800). whereas heterologous reaction with /v. alhinolticus decreased from 1:3,200 to 1:200. Flagella antiserum against V. parahaemlyticus did not reacted with H-antigens of other Vibrio specices. Both absorbed anti-WCA serum and flagella antiserum were reacted specifically with 32 stains of V. parahaemoluticus isolated from patients other specimens. This result indicated that the antisera against whole cell antigen of V. parahaemolyticus followed by absorption with homologus O-antigen and H antigen V. alginolyticus and flagella antigen is preferable to O-antisera in respect of sensitivity and specificity.
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국내 설사환자로부터 분리된 Escherichia coli 균의 혈청군 및 Verocytotoxin 생성능
김호훈,강연호,김성한,박미선,유재연,이복권 대한감염학회 1998 감염 Vol.30 No.5
목적: 국내 설사 환자에게서 분리 동정된 인체 감염 유래 E. coli 균의 혈청군, verocytotoxin 생성능을 구명하여 EHEC 균의 인체 감염 실태를 구명하고, 동물 및 식품 유래 분리균주의 성상과 비교 검토하여 인체 감염 위험성에 대한 기초적 자료를 의학계에 제공하고자 하였다. 방법: EHEC 감염의심 설사 환자 분변 검체로부터 분리된 균주 중 생화확적 성상에 따라 대장균을 분리하였고 이들 균주 중 E. coli O157:H7를 분리하기 위해서는 Dsorbitol 음성균을 선별하였으며, 분리균에 대하여 항혈청으로 응집 시험을 실시하여 혈청군을 구명하였다. 혈청군이 확인된 균주에 대하여 중합효소 연쇄반응 (PCR) 및 역수동라텍스 응집시험 (Reversed Passive Latex Agglutination:RPLA) 으로 verocytotoxin 생성능을 구명하였다. 결과: 장출혈성 대장균 감염 의심설사 환자의 분변검체로부터 25주의 E. coli 균을 분리 동정하였으나 이들 균주는 모두 verocytotoxin을 생성하지 않았다. 분리 동정된 대장균으로부터 확인된 혈청군은 16종으로서 E. coli O1, O6, O8, O15, O20, O25 O26, O28, O29, O44, O86a, O119, O126, O128, O152, 및 O157:H-였으며 E. coli O157:H-혈청형 균주와 E.coli O25 혈청군 균주가 각각 3주씩 분리되어 비교적 높은 분리율을 보였다. 결론: 장출혈성 대장균 감염 의심설사 환자 분변 가검물로부터 총 25주의 E. coli균을 분리 동정하였으나 verocytotoxin을 모두 생산하지 않았다. Background: Since 1982, many countries has reported outbreaks or sporadic cases caused by enterohaemorrhagic Escherichia coli (EHEC) serogroup strains, mainly E. coli O157:H7 type strain. However, systemic investigation about EHEC agents, including E. coli O157:H7, have not been done in Korea. Therefore, we investigated serogroup and verotoxin productivity of E. coli strains isolated from diarrheal patients and estimated risk of human infection in comparison with EHEC strains isolated from cow, pig, and food material in Korea. Methods: Diarrheal patient stool samples were collected and E. coli strains were isolated, according to biochemical characteristics. In order to isolate E. coli O157:H7, D-Sorbitol negative strains were selected. Serogrouping of the E. coli isolates was done by agglutination test. Verocytotoxin productivity was investigated by polymerase chain reaction (PCR) and reversed passive latex agglutination (RPLA). Human infection risk was estimated in comparison with EHEC strains isolated from cow, pig and food materials in Korea. Results: Twenty-five E. coli strains were isolated from the diarrheal patients who were suspected to be infected with EHEC. However, none of these E. coli strains produced verocytotoxin. Out of 25 E. coli isolates, 16 serogroups of E. coli O1, O6, O8, O15, O20, O25, O26, O28, O29, O44, O86a, O119, O126, O128, O152 and 157:H-were found. In each of the E. coli O157:H-and O25 serogroups 3 strains were found. Conclusion: None of 25 E. coli isolated from diarrheal patients who were suspected of EHEC infection produced verocytotoxin producing E. coli have been reported recently in Korea.
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