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김혜진,김광준,조혜원,진태호,Kim Hye-Jin,Kim Kwang-Jun,Cho Hye-Won,Jin Tai-Ho 대한치과보철학회 2002 대한치과보철학회지 Vol.40 No.1
The purpose of this study was to investigate the color stability of composite resins used widely as esthetic restorative material. Surefil(Dentsply, U.S.A.), Esthet X(Dentsply, U.S.A.), Filtek P60(3M, U.S.A.) , Filtek Z250(3M, U.S.A.), Synergy Compact(Coltene, Switzerland), and Synergy Duo(Coltene, Switzerland) were chosen for this study. Thirty six specimens($7mm{\times}2mm$) of each composite resin were fabricated and polished after polymerization. Treatment conditions designed for this study were thermocycling, methylene blue staining, and filtered coffee staining. The color changes before and after treatment were measured by colorimeter(colorimeter, Model Tc-6Fx, Tokyo Denshoku Co.) and analyzed. The followings were drawn from this study: 1. The color changes of Filtek Z250, Surefil, Filtek P60 and Esthet X after thermocycling for 3 weeks were greater than those of the others. 2. The color changes of Surefil and Esthet X after methylene blue stainig and those of Surefil, Filtek Z250 and Filtek P60 after coffee staining were great. 3. The color changes or Synergy Duo and Synergy Compact after thermocycling and coffee staining were relatively lesser than those of other composite resins.
김혜진,박재완,박하주,김덕규,설우준,Kim, Hye-Jin,Park, Jae Wan,Park, Ha Ju,Kim, Dockyu,Sul, Woo Jun The Microbiological Society of Korea 2016 미생물학회지 Vol.52 No.3
부식산(humic acid)을 이용하여 저온에서 배양된 Pedobacter sp. PAMC 27299는 남극 바톤 반도(Barton Peninsula) King George Island의 해안가 이끼(moss debris)로부터 분리되었다. 본 연구에서는 Pedobacter sp. PAMC 27299의 유전체 서열을 해독하였으며 크기 6,147,290 bp, G+C 함량 40.54%의 PAMC 27299는 지구 온난화 관련 저온적응 부식산 분해 효소를 보유하는 것으로 확인되었다. Pedobacter sp. PAMC 27299 with humic acid cultivated on low temperature was isolated from the moss debris on the coast of the Barton Peninsula of King George Island of the maritime Antarctic region. Here, we present the complete genome sequence of Pedobacter sp. PAMC 27299, which contains 6,147,290 bp with a G+C content of 40.54%. PAMC 27299 may possess cold-adapted humic acid degradation enzymes with implication on global warming.
식이성 폴리페놀 (-)-epigallocatechin-3-gallate가 mouse C2C12 myoblast 분화에 미치는 영향
김혜진,이원준,Kim, Hye-Jin,Lee, Won-Jun 한국생명과학회 2007 생명과학회지 Vol.17 No.3
본 연구에서는 유전자 발현에 중요한 조절 역할을 하는 DNA 메틸화를 식이성 폴리페놀의 하나인 녹차의 대표적인 추출물 EGCG로 억제하였을 때 C2C12 myoblast 세포에 일어나는 현상을 살펴보았다. 그 결과 smooth muscle의 지표인 transgelin, smooth muscle ${\alpha}-actin$ mRNA와 단백질이 현저히 증가함을 보였고, 형태학적으로도 smooth muscle의 전형적인 모습을 보였다. 식이에 포함된 DNA 메틸화 억제제인 EGCG가 C2C12 myoblast를 smooth muscle로 분화를 유도하였으며, 암 예방 차원에서의 EGCG의 역할 외에 혈관질환과 같은 smooth muscle에 관련된 예방과 치료차원에서 EGCG의 역할이 있을 것으로 사료된다. 본 연구는 C2C12 myoblast를 smooth muscle로 유도하는 결정적인 신호전달 역할을 하는 유전자에 대한 연구는 수행하지 못하였다. 따라서 EGCG에 의해 변화되는 유전자에 대한 기전연구가 필요하다고 하겠다. In the present investigation, we studied the modulating effects of (-)-epigallocatechin-3-gallate(EGCG) on the differentiation of mouse C2C12 myoblasts. We found that the strong inhibitory effect of EGCG on DNA methyltransferase-mediated DNA methylation induced transdifferentiation of C2C12 myoblasts into smooth muscle cells demonstrated by both morphological changes and immunofluorescent staining. C2C12 myoblasts treated with EGCG for 4 days expressed smooth muscle ${\alpha}-actin$ protein. Real-time PCR data revealed that smooth muscle ${\alpha}-actin$ mRNA was induced by EGCG treated C2C12 myoblasts in a concentration-dependent manner. Smooth muscle ${\alpha}-actin$ mRNA concentration increased 330% and 490% after 2 and 3 days of 50 ${\mu}M$ of EGCG treatment. The expression of another smooth muscle marker, transgelin, mRNA was also increased up to 9-fold by 4 days of EGCG treatment compared with control in a concentration-dependent manner. These results suggested that C2C12 enables to transdifferentiate into smooth muscle when gene expression patterns are changed by the inhibition of DNA methylation induced by EGCG. In conclusion, transdifferentiation of C2C12 myoblasts into smooth muscle is resulted from the modulating effects of EGCG on DNA methylation which subsequently results in changing the expression pattern of several genes playing a critical role in the differentiation of C2C12 myoblasts.