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3기통 스파크 점화기관의 흡ㆍ배기관내에서의 압력변화에 관한 연구
김형기(H.K.KIM),전광민(K.M.CHUN) 한국자동차공학회 1992 한국자동차공학회 춘 추계 학술대회 논문집 Vol.1992 No.11
The purpose of this study is to investigate the flow through the intake and exhaust manifolds in a three-cylinder spark-ignition engine.<br/> The flow in the intake and exhaust manifolds was calculated using the characteristic method and the gas properties in the cylinder were calculated using the first law of thermodynamics.<br/> In the experiment, using four-stroke three-cylinder spark-ignition engine, the pressure in the intake and exhaust manifolds was measured and compared with the result of numerical analysis.<br/> From the comparison, following conclusion was obtained. The results obtained from the experiment were in accord with those from calculation. In the intake manifold, pressure wave which has three peaks per cycle was observed in both cases of experiment and calculation. The three peaks were combined with many small peaks reflected from the bended intake manifold wall. In the exhaust manifold, pressure wave per cycle has six peaks in both cases of experiment and calculation. The large peaks occur at blowdown phase and the small peaks occur at displacement phase.
Histidine rich-region의 발현에 의한 효모에서의 구리함량 증가
김형기,문성현,김우현 中央大學校 遺傳工學硏究所 2002 遺傳工學硏究論集 Vol.15 No.1
Copper is an essential metal for all types of organisms and is involved in many biological activities in cells. However, an excessive amount of copper is toxic to cells. In Saccharomyces cerevisiae, the knowledge of the homeostatic mechanism at the stage of copper uptake into cells has greatly increased during the past few years. In this study, cloning and expression of copper-binding peptide gene in S. cerevisiae was carried out to enhance the copper-chelation capacity of yeast. S. cerevisiae DBY747 was transformed with pDO-his vector containing the DNA encoding histidine rich-region, and the copper content of DBY747 harboring the vector was measured. Intracellular copper content of DBY747 harboring pDO-his vector, which contains 9 hexa-histidine unit DNA, was about 31,000 ppm after culturing in the presence of 3 mM CuSO_4, whereas those DBY747 and DBY747 harboring pDO105 vector were 840 ppm and 1,020 ppm, respectively.