Saccharomyces cerevisiae에서 Cycloinulooligosaccharide Fructanotransferase 유전자의 표층 발현

김현진,이재형,김현철,김연희,권현주,남수완,Kim, Hyun-Jin,Lee, Jae-Hyung,Kim, Hyun-Chul,Kim, Yeon-Hee,Kwon, Hyun-Ju,Nam, Soo-Wan 한국생명과학회 2007 생명과학회지 Vol.17 No.2

Paenibacillus macerans 유래의 cycloinulooligosaccharide fructanotransferase (CFTase) 유전자(cft, 2832 bp, 103.8 kDa)를 효모 표층발현 vector인 pCTcon (GAL1 promoter)에 subcloning 하였다. 구축된 pCTECFTN (9.0 kb)를 숙주세포인 s. cerevisiae EBY100에 형질전환한 후, uracil이 결핍된 배지와 inulin 함유배지에서 선별하였다. cft 유전자는 GAL1 promoter에 의해 효모 형질전환체에서 성공적으로 발현되었다. inulin으로부터 cyclofructans (CFs)로 생산하는 효소적 능력으로부터 표층발현 유무를 확인하였다. YPDG배지에서 48시간 배양 후 분획한 균체는 5.52 unit/ l 의 활성을 보였다. CF 생산을 위한 효소의 최적 반응 조건으로 pH 8.0, 반응온도 $50^{\circ}C$, 기질농도 5%, 기질은 Jerusalem artichoke 등의 inulin과 표층 발현 CFTase 효소반응 결과, cycloinulohexaose (CF6), cycloinuloheptaose (CF7), 그리고 cycloinulooctaose (CF8)이 생성되었고, 이 중에서 CF6가 주 생성물이었다. The cycloinulooligosaccharide fructanotransferase (CFTase) gene (cft) from Paenibacillus macerans was subcloned into the surface display vector, pCTcon (GAL1 promoter). The constructed plasmid, pCTECFTN (9.0 kb) was introduced to S. cerevisiae EBY100 cell and then east transformants were selected on the synthetic defined medium lacking uracil and on the inulin containing medium. The surface display of CFTase was confirmed by immunofluorescence microscopy and its enzymatic ability to form cycloinulooligosaccharides(cyclofructans, CFs) from inulin. The total activity of the CFTase was reached about 5.52 unit/1 by cultivation of yeast transformant on YPDG medium. The optimized conditions determined were as follows; pH, 8.0; temperature, $50^{\circ}C$ ; substrate concentration, 5%; inulin source, Jerusalem artichoke. By the reaction with inulin, CFs consisting of cycloinulohexaose (CF6), cycloinuloheptaose (CF7), and cycloinulooctaose (CF8) were produced and CF6 was the major product.

감모후유증(感冒後遺症)으로 기허형(氣虛型)호흡기 질환을 호소하는 환자의 한약치료에 관한 임상연구

김현진,한효정,장정아,박은영,안태한,서호석,김진원,Kim, Hyun-Jin,Han, Hyo-Jung,Jang, Jeong-A,Park, Eun-Young,An, Tae-Han,Seo, Ho-Seok,Kim, Jin-Won 대한한의학방제학회 2010 大韓韓醫學方劑學會誌 Vol.18 No.1

Objectives : In order to confirm a remedial effect and related influence of the clinic treatment by prescribing Samchuljojung-tang(蔘朮調中湯), Bojungikqi-tang(補中益氣湯) and Samso-um(蔘蘇飮) to patients who suffering from a chronic cough and rhinorrhea. Methods : 1. In a clinical observation and analysis method, we have selected the 23 patients who have visited National Oriental Medical Center, date from 2007.8.21 to 2008.7.31 and suffering from a chronic cough and rhinorrhea caused by the common cold sequelae. 2. Separated them into three types(I: cough type, II: rhinorrhea type, III: cough & rhinorrhea type). 3. Observed thoroughly the level of symptom and recorded it for each type with questionnaire survey at the beginning set 4. Prescribed Samchuljojung-tang, Bojungikqi-tang and Samso-um for type I, II, III respectively. 5. Observed the level of symptom and treatment afterwards affect, and recorded movements of intensity level for 3 types with same questionnaires. Results : 1. Type I : The survey turned out to be from 9 patients, initial level recorded 31.89 at the entry diagnosis, and next level that means changing of symptoms, recorded to 12.67 after prescribing herb medication. 2. Type II : From 11 patients, initial level was 32.82 and next level was 13.45. 3. Type III : From 3 patients, initial level was 37.67 and next one was 16.00. Conclusions : It is concluded that there is a significant remedial effect and related influence of the clinic treatment for each type of patients who suffering from a chronic cough and rhinorrhea caused by the common cold sequelae.

Burden of Disease Due to Outdoor Air Pollution in Korea: Based on PM₁₀

김현진,윤석준,김형수,이건세,김은정,조민우,오인환,Kim, Hyun-Jin,Yoon, Seok-Jun,Kim, Hyeong-Su,Lee, Kun-Sei,Kim, Eun-Jung,Jo, Min-Woo,Oh, In-Hwan Korean Society of Environmental Health 2011 한국환경보건학회지 Vol.37 No.5

Purpose: This study measured the burden of disease in Korea related to outdoor air pollution using disability-adjusted life year (DALY). Materials and Methods: As a risk factor of outdoor air pollution, particulate matter with a diameter less than 10 ${\mu}m$($PM_{10}$) was used. First, $PM_{10}$-related diseases and their relative risk (RR) were selected by means of a literature review. Second, population attributable fractions were computed by using formulae including RR and population exposure to $PM_{10}$. Third, DALYs of $PM_{10}$-related diseases in Korea were estimated. Finally, the attributable burden of disease due to $PM_{10}$ was measured as the sum of the products that multiplied the DALYs of $PM_{10}$-related diseases by their population attributable fractions. Results: The disease burden of PM10 was 6.9 DALY per 1,000 persons in 2007. The attributable burden of $PM_{10}$ was 2.68 for lung cancer, 2.41 for COPD, 0.62 for ischemic heart disease, 0.61 for pneumonia, 0.55 for asthma, and 0.03 for preterm. Conclusions: This study showed the environmental burden of disease of $PM_{10}$ and burden of $PM_{10}$-related disease through objective data. It also suggested that active efforts are needed to continuously measure and reduce the burden of environmental diseases in Korea.

다중 주사 경로 회로 기판을 위한 내장된 자체 테스트 기법의 연구

김현진,신종철,임용태,강성호,Kim, Hyun-Jin,Shin, Jong-Chul,Yim, Yong-Tae,Kang, Sung-Ho 대한전자공학회 1999 電子工學會論文誌, C Vol.c36 No.2

The IEEE standard 1149.1, which was proposed to increase the observability and the controllability in I/O pins, makes it possible the board level testing. In the boundary-scan environments, many shift operations are required due to their serial nature. This increases the test application time and the test application costs. To reduce the test application time, the method based on the parallel opereational multiple scan paths was proposed, but this requires the additional I/O pins and the internal wires. Moreover, it is difficult to make the designs in conformity to the IEEE standard 1149.1 since the standard does not support the parallel operation of data shifts on the scan paths. In this paper, the multiple scan path access algorithm which controls two scan paths simultaneously with one test bus is proposed. Based on the new algorithm, the new algorithm, the new board level BIST architecture which has a relatively small area overhead is developed. The new BIST architecture can reduce the test application time since it can shift the test patterns and the test responses of two scan paths at a time. In addition, it can reduce the costs for the test pattern generation and the test response analysis. 인쇄 회로 보드 수준의 테스팅을 위해 제안된 IEEE 표준 1149.1은 보드상의 테스트 지점에 대한 제어용이도와 관측용이도를 향상시켜 보드의 테스트를 용이하게 해준다. 그러나, 경계 주사 환경에서는 테스트 입력과 테스트 결과에 따른 데이터가 하나의 주사 연결에 의해서 직렬로 이동된다. 이는 테스트 적용시간을 증가시키고 따라서 테스트에 드는 비용을 증가시킨다. 테스트에 소모되는 시간을 줄이기 위해 병렬로 다중주사 경로를 구성하는 방법이 제안되었다. 하지만 이는 여분의 입출력 핀과 내선을 필요로 한다. 더구나 IEEE 표준 1149.1은 주사 경로 상에 있는 IC들의 병렬 동작을 지원하지 않기 때문에 표준에 맞게 설계하기가 어렵다. 본 논문에서는 하나의 테스트 버스로 두 개의 주사 경로를 동시에 제안하는 다중 주사 경로 접근 알고리즘에 기초하여 적은 면적 오버헤드를 가지고 빠른 시간 내에 보드를 테스트할 수 있는 새로운 보드수준의 내장된 자체 테스트 구조를 구현하였다. 제안된 내장된 자체 테스트 구조는 두 개의 주사 경로에 대한 테스트 입력과 테스트 결과를 이동시킬 수 있으므로 테스트에 소모되는 시간을 줄일 수 있고 또한 테스트 입력의 생성과 테스트 결과의 분석에 소모되는 비용을 줄일 수 있다.

농촌노인 여가복지시설 이용 실태 및 만족도에 관한 연구 - 의성군 지역을 중심으로 -

김현진,김용범,Kim, Hyun-Jin,Kim, Yong-Bum 한국주거학회 2010 한국주거학회 논문집 Vol.21 No.5

Although the government financial aid is insufficient and the lack of programs and facilities prevent it from developing and activation in the rural local society, welfare centers for the aged are formed an ever-greater part of the leisure and welfare facility for the aged in rural areas. As a result, the objectives of this study are to investigate and identify the actual conditions of use by the aged and the degree of satisfaction for welfare centers for the aged in Uiseong district. The results of this study are as follows. In the survey sample characteristics based on a total of 303 survey, the percentage of women (55.2%) was much higher than men (44.8%), average age is 73.3 years old. According to the results of the analysis, most important motivation is for health and making friends, and major useful facilities and programs are related to physical activities, sing a song and rehabilitation. From the results of the overall satisfaction, positive promotions and sufficient aid are required for the aged in the rural local society. Further any programs developed internally are required to promote and activate the leisure activity programs for the aged.

커뮤니티를 고려한 주거환경개선요소 설정 및 중요도 분석

김현진,구자훈,원제무,Kim, Hyun-Jin,Koo, Ja-Hoon,Won, Jai-Mu 한국주거학회 2009 한국주거학회 논문집 Vol.20 No.6

This study initially analyzes the meaning of Community-Based Residential Environment Improvement. A residential environment improvement factor was determined through an analysis of the function, components, Planning techniques of the community, the evaluation criteria of the residential environment, and through relative literature documenting plans for the adjustment of urban and housing environments. In terms of the methodological approach, decisions were made based on the results of an expert focus-group-interview, a goodness-of-fit test, and by using the analytic network decision-making method from which the correlation among items can be considered. Results of this analysis show that the, order of importance of factors for a community-based residential environment is as follows: interactional character, cognitive character, and locational character. Furthermore, the most important aspect for each of these factors are the neighbor-hood relationship in terms of the interactional character, common consciousness for the cognitive character and place for locational character. The results of this study can be used by linking them with questionnaire items for basic resident research when a residential environment improvement project or a plan for the adjustment of urban and housing environments is planned.

안과에 의뢰된 신체 감정 환자들의 임상 양상 분석

김현진,이현주,임기환,Hyun-Jin Kim,M,D,Hyun-Joo Lee,M,D,Key-Hwan Lim,M,D 대한안과학회 2008 대한안과학회지 Vol.49 No.5

Purpose: To investigate the clinical characteristics and disability rates of patients seeking expert consultation for legal reasons because of ophthalmologic symptoms. Methods: We analyzed the gender, age, reason for consultation, and result of consultation, among other factors, by retrospectively examining the medical files of 89 patients from January 1999 to December 2004. Results: Out of 89 patients, men constituted 76.4% and patients in their forties constituted 23.0%. The most common reason for consultation was a traffic accident (64.0%), followed by a medical lawsuit (11.2%). Forty-nine patients (55.1%) were diagnosed with a disability, while 30 patients (33.7%) were not. Diagnostic decisions for the other 10 patients (11.2%) were deferred. Optic atrophy was the most frequent cause for deteriorating visual acuity in patients, accounting for 19 cases, and diplopia caused by orbital wall fracture was also prevalent (9 patients). Among the resulting types of damage, brain damage was the most frequent, while the percentage of patients with an impairment in the visual system was approximately 29.0% among the patients diagnosed with a disability. Conclusions: Our results will be helpful in elucidating the clinical characteristics of patients referred to the ophthalmic clinic for consultation because of legal concerns.

반성적 마이크로티칭과 비원어민 예비 영어 교사의 외국어 교수 불안감

김현진,Kim, Hyun-Jin 한국영어어문교육학회 2009 영어어문교육 Vol.15 No.4

The present data-driven study attempted to explicate nonnative English teacher trainees' foreign language teaching anxiety in microteaching settings from their perspectives. It is assumed that nonnative English teachers or teacher trainees may experience anxiety not only as foreign language learners but also as foreign language teachers. In order to inquire into their anxiety, the researcher had 172 teacher trainees perform extended microteaching tasks and reflect on their teaching and anxiety through group discussion. Based on the analysis of their discussion, three aspects related to nonnative English teacher trainees' anxiety were identified. First, teacher trainees identified three main types of anxiety-provoking situations: communicative-competence-threatening situations, unexpected situations, and instruction-hindering situations. Second, they identified three sources of anxiety: limited ability to use English, lack of English teaching skills, and fear of criticism. Third, they were aware that they used diverse strategies to lower anxiety before and while teaching for different purposes. From their identification and awareness of anxiety-provoking situations, sources of anxiety, and anxiety-lowering strategies, they could reflect on professional qualifications as a foreign language teacher.

Ultrastructural Charateristics of a Human Sialolith

김현진,이수근,서봉직,Kim, Hyun-Jin,Lee, Soo-Guen,Suh, Bong-Jik The Korean Academy of Orofacial Pain and Oral Medi 1999 Journal of Oral Medicine and Pain Vol.24 No.4

타석에 관한 연구는 타석증을 보이는 환자에 대한 임상적 특징, 진단 및 치료에서부터 타석의 성분 및 구조 등에 이르기까지 다양한 범위에 걸쳐 이루어지고 있다. 타석의 미세구조에 관한 연구는 타석의 미세구조가 다양한 형태인 것으로 보고되고 있으며, 특히 최근 타석증의 치료에 새롭게 소개되고 있는 체외충격파쇄석술은 타석의 구조에 따라 그 효과가 영향을 받을 수 있을 것으로 사료된다. 이에 저자는 인간 타석의 미세구조에 관한 기본 자료가 필요할 것으로 사료되어 한국인 중년 여성으로부터 적출된 악하선 타석을 광학현미경 및 주사전자현미경을 이용하여 미세구조적 특징을 관찰한 결과, 다음과 같은 결론을 얻었다. 1. 타석은 중심부의 핵, 핵 주변의 층상구조 및 외피막으로 이루어져 있었다. 2. 핵은 비정형의 중심과 상대적으로 균질의 외곽부위로 구성되어 있었다. 3. 핵 주변은 대부분 동심원적인 층상구조를 보였지만 일부분에서는 균질의 구조를 보였다. 4. 타석 단면의 전체직경과 중심부 핵의 직경은 각각 $3,500{\mu}m$와 $1,500{\mu}m$였고, 층상구조를 이루는 각 층의 두께는 위치에 따라 약 $10{\sim}40{\mu}m$ 이내였다.

혼합용매 내에서 단분산 Al2O3 미립자의 합성에 대한 전해질 첨가효과

김현진,김희택,배성렬,류경옥 ( Hyun Jin Kim,Hee Taik Kim,Seong Youl Bae,Kyong Ok Yoo ) 한국화학공학회 1995 화학공학의이론과응용 Vol.1 No.2