Saccharomyces cerevisiae에서 Cycloinulooligosaccharide Fructanotransferase 유전자의 표층 발현

김현진,이재형,김현철,김연희,권현주,남수완,Kim, Hyun-Jin,Lee, Jae-Hyung,Kim, Hyun-Chul,Kim, Yeon-Hee,Kwon, Hyun-Ju,Nam, Soo-Wan 한국생명과학회 2007 생명과학회지 Vol.17 No.2

Paenibacillus macerans 유래의 cycloinulooligosaccharide fructanotransferase (CFTase) 유전자(cft, 2832 bp, 103.8 kDa)를 효모 표층발현 vector인 pCTcon (GAL1 promoter)에 subcloning 하였다. 구축된 pCTECFTN (9.0 kb)를 숙주세포인 s. cerevisiae EBY100에 형질전환한 후, uracil이 결핍된 배지와 inulin 함유배지에서 선별하였다. cft 유전자는 GAL1 promoter에 의해 효모 형질전환체에서 성공적으로 발현되었다. inulin으로부터 cyclofructans (CFs)로 생산하는 효소적 능력으로부터 표층발현 유무를 확인하였다. YPDG배지에서 48시간 배양 후 분획한 균체는 5.52 unit/ l 의 활성을 보였다. CF 생산을 위한 효소의 최적 반응 조건으로 pH 8.0, 반응온도 $50^{\circ}C$, 기질농도 5%, 기질은 Jerusalem artichoke 등의 inulin과 표층 발현 CFTase 효소반응 결과, cycloinulohexaose (CF6), cycloinuloheptaose (CF7), 그리고 cycloinulooctaose (CF8)이 생성되었고, 이 중에서 CF6가 주 생성물이었다. The cycloinulooligosaccharide fructanotransferase (CFTase) gene (cft) from Paenibacillus macerans was subcloned into the surface display vector, pCTcon (GAL1 promoter). The constructed plasmid, pCTECFTN (9.0 kb) was introduced to S. cerevisiae EBY100 cell and then east transformants were selected on the synthetic defined medium lacking uracil and on the inulin containing medium. The surface display of CFTase was confirmed by immunofluorescence microscopy and its enzymatic ability to form cycloinulooligosaccharides(cyclofructans, CFs) from inulin. The total activity of the CFTase was reached about 5.52 unit/1 by cultivation of yeast transformant on YPDG medium. The optimized conditions determined were as follows; pH, 8.0; temperature, $50^{\circ}C$ ; substrate concentration, 5%; inulin source, Jerusalem artichoke. By the reaction with inulin, CFs consisting of cycloinulohexaose (CF6), cycloinuloheptaose (CF7), and cycloinulooctaose (CF8) were produced and CF6 was the major product.

Burden of Disease Due to Outdoor Air Pollution in Korea: Based on PM₁₀

김현진,윤석준,김형수,이건세,김은정,조민우,오인환,Kim, Hyun-Jin,Yoon, Seok-Jun,Kim, Hyeong-Su,Lee, Kun-Sei,Kim, Eun-Jung,Jo, Min-Woo,Oh, In-Hwan Korean Society of Environmental Health 2011 한국환경보건학회지 Vol.37 No.5

Purpose: This study measured the burden of disease in Korea related to outdoor air pollution using disability-adjusted life year (DALY). Materials and Methods: As a risk factor of outdoor air pollution, particulate matter with a diameter less than 10 ${\mu}m$($PM_{10}$) was used. First, $PM_{10}$-related diseases and their relative risk (RR) were selected by means of a literature review. Second, population attributable fractions were computed by using formulae including RR and population exposure to $PM_{10}$. Third, DALYs of $PM_{10}$-related diseases in Korea were estimated. Finally, the attributable burden of disease due to $PM_{10}$ was measured as the sum of the products that multiplied the DALYs of $PM_{10}$-related diseases by their population attributable fractions. Results: The disease burden of PM10 was 6.9 DALY per 1,000 persons in 2007. The attributable burden of $PM_{10}$ was 2.68 for lung cancer, 2.41 for COPD, 0.62 for ischemic heart disease, 0.61 for pneumonia, 0.55 for asthma, and 0.03 for preterm. Conclusions: This study showed the environmental burden of disease of $PM_{10}$ and burden of $PM_{10}$-related disease through objective data. It also suggested that active efforts are needed to continuously measure and reduce the burden of environmental diseases in Korea.

음파 가진을 이용한 2차원 웨이크 유동 제어에 관한 연구

김현진,김재호,김명균,Kim, Hyeon-Jin,Kim, Jae-Ho,Kim, Myeong-Gyun 대한기계학회 1998 大韓機械學會論文集B Vol.22 No.6

In a low speed open-type wind tunnel, a group of parallel wakes downstream of two dimensional grid model consisting of several circular cylinders were experimentally investigated to study the response of the wake flows to the acoustic excitation, in hoping to promote the understanding of the underlying mechanism behind the gross flow change due to artificial excitation. In the unexcited wake flows, the development of the individual wakes behind cylinders was almost uniform for the ratio of the spacing to the cylinder diameter of s/d.geq.1.5. For smaller s/d, however, the jet streams issued through the gaps between the cylinders became biased in one side and the cylinders had wakes of different sizes. At s/d=1.25, the gap flow directions change in time, leading to unstable wake patterns. Further reduction in s/d made this unstable flip-flopping of the jets stable. The most effective excitation frequency was found to be in the Strouhal number range of St=0.5-0.6. This frequency was related to the vortex shedding. At s/d=1.75, the excitation frequency was 2 or 4 times the vortex shedding frequency. When the flow was excited at this frequency, the vortex sheddings were energized, and pairings between neighboring vortices were generated. Also, the merging process between individual wakes was accelerated. The unstable and unbalanced wake patterns at s/d=2.15 were made stable and balanced. The unstable and unbalanced wake patterns at s/d=2.15 were made stable and balanced. For smaller spacing of s/d .leq,1.0, the acoustic excitation became less effective in controlling the flow.

Distribution of Aquaporins (Water Channels) in the Rat Salivary Glands

정지연(Ji-Yeon Jung),한창룡(Chang-Ryoung Han),정연진(Yeon-Jin Jeong),오원만(Won-Mann Oh),김미원(Mi-Won Kim1),김선헌(Sun-Hun Kim),김옥준(Ok-Joon Kim),김현진(Huyn-Jin Kim),고정태(Jeong-Tae Koh),최홍란(Hong-Ran Choi),김원재(Won-Jae Kim) 대한해부학회 2002 Anatomy & Cell Biology Vol.35 No.3

침샘은 하루 1.5 l의 침을 분비하며, 일반적으로 침샘에서 수분이동은 능동적 염분 이동에 따른 삼투현상에 의해 일어난다. 따라서 침샘에서 높은 수분투과성으로 인해 수분통로 단백인 aquaporin (AQP)들이 풍부하게 존재할 수 있다. 지금까지침샘에 4가지 형태의 AQP이 존재한다고 알려져 있지만 침샘에서 정확한 위치와 수분 이동에 대한 각 AQP들의 역할은 아직 확실치 않다. 본 연구는 300 g 정도의 흰쥐를 pentobarbital sodium (50 mg/kg, IP) 마취하에서 carbarchol (10 μg/kg)을 복강 내 투여한 후 AQP들의 역할과 분포를 면역조직화학방법으로 조사하였다. AQP1은 침샘의 미세혈관의 내피세포와 샘세포와 샘관 주위에 존재하는 근육상피세포에 존재하였다. AQP4는 침샘관의 마지막 부위인 배출관에 존재하였다. AQP5는 주로 장액세포의 바닥가쪽과 세포사이 분비소관을 포함한 세포꼭대기쪽막 에 존재하였으며 콜린성 분비 자극으로 세포꼭대기쪽막으로 이동되어 밀집되었다. AQP5는 모든 침샘의 사이관과 줄무늬 관에도 존재하였으며 점액세포는 약하게 존재하였다. AQP8은 AQP5처럼 장액세포의 바닥쪽막과 세포사이 분비소관을 포함한 세포꼭대기쪽막에 존재하였으며 콜린성 침분비 자극으로 세포꼭대기쪽막쪽으로 이동되어 밀집되었다. 이상의 실험결과는 AQP5와 AQP8이 장액세포에서 일차 침 형성시 일어나는 수분이동의 주된 통로임을 시사하였다. The salivary glands produce 1.5 l of fluid per day. As in other organs, the general paradigm in the salivary glands is that water movement occurs secondary to osmotic driving forces created by active salt transport. Therefore, high water permeability in salivary glands is expected to need a variety of aquaporin (AQP), a water channel. Although four AQPs have been known to reside in salivary glands, the precise location and roles of AQPs have been not well examined. This study is aimed to investigate the distribution of AQPs in 3 major salivary glands and their changes after cholinergic stimulation using immunohistochemical study in Sprague Dawley rats weighing 300 g under pentobarbital sodium anesthesia. AQP1 was localized in the endothelial cells of all salivary capillary vessels and the myoepithelial cells. AQP4 was demonstrated in the epithelium of the excretory ductal cells of all salivary glands. AQP5 and 8 were abundantly present in the basolateral membrane and apical membranes of the serous acini including intercellular secretory canaliculi, whereas AQP5 was weakly present in mucous acini. In addition, AQP5 was found in the epithelium of the intercalated and striated ducts. Upon stimulation of carbachol (10 μg/kg, I.P). AQP5 and 8 tended to translocate from basolateral membrane to the apical membrane, appearing as clusters of dots. These results suggest that AQP5 and 8 are the candidate molecules responsible for the water movement in salivary acinar cells.

고유가 정착 및 교토의 정서 발효가 기업에 미치는 영향 및 대응

김현진,Kim, Hyeon-Jin 대한석유협회 2005 석유와 에너지 Vol.2005 No.3

현장과 학계 연계를 통한 사회복지 교육

김현진,Kim, Hyeon-Jin 한국사회복지사협회 2005 Social workers Vol.8 No.-

가속화되는 석유의 '정치화'와 국가 에너지 안보 전략의 필요성

김현진,Kim, Hyeon-Jin 대한석유협회 2004 석유와 에너지 Vol.2004 No.7

케틀벨 운동과 초음파 치료가 20대 성인의 체질량지수, 체지방률에 미치는 영향

김현진,김명훈,Kim, Hyun-Jin,Kim, Myung-Hoon 대한물리치료과학회 2018 대한물리치료과학회지 Vol.25 No.2

Background: This study was to examine the influence of BMI and PBF of adults in 20's through the Kettlebell exercise and ultrasound for 4 weeks. Methods: 24 adults men and women, whose BMI is over 23, was divided into 3 groups, group I (control group), group II (Kettlebell exercise group), group III (ultrasound treatment and Kettlebell exercise group). The ultrasound treatment was done before exercise for 15 minutes 3 times a week for 4 weeks, and exercise worked out with Kettlebell exercise during 15 minutes and were stretched for 10 minutes before and after exercise. Results: For 4 weeks of Kettlebell exercise and ultrasound, there was a statistically meaningful difference between group I and group III (p<.05). For 4 weeks, there was no statistically meaningful difference of BMI and PBF between group II and group. I Conclusion: A complex application of Kettlebell exercise and ultrasound treatment was thought to effective decreasing for BMI and PBF.

냉매압축기 기술동향

김현진,Kim, Hyeon-Jin 한국설비기술협회 1994 설비 : 공조ㆍ냉동ㆍ위생 Vol.11 No.7

안과에 의뢰된 신체 감정 환자들의 임상 양상 분석

김현진,이현주,임기환,Hyun-Jin Kim,M,D,Hyun-Joo Lee,M,D,Key-Hwan Lim,M,D 대한안과학회 2008 대한안과학회지 Vol.49 No.5

Purpose: To investigate the clinical characteristics and disability rates of patients seeking expert consultation for legal reasons because of ophthalmologic symptoms. Methods: We analyzed the gender, age, reason for consultation, and result of consultation, among other factors, by retrospectively examining the medical files of 89 patients from January 1999 to December 2004. Results: Out of 89 patients, men constituted 76.4% and patients in their forties constituted 23.0%. The most common reason for consultation was a traffic accident (64.0%), followed by a medical lawsuit (11.2%). Forty-nine patients (55.1%) were diagnosed with a disability, while 30 patients (33.7%) were not. Diagnostic decisions for the other 10 patients (11.2%) were deferred. Optic atrophy was the most frequent cause for deteriorating visual acuity in patients, accounting for 19 cases, and diplopia caused by orbital wall fracture was also prevalent (9 patients). Among the resulting types of damage, brain damage was the most frequent, while the percentage of patients with an impairment in the visual system was approximately 29.0% among the patients diagnosed with a disability. Conclusions: Our results will be helpful in elucidating the clinical characteristics of patients referred to the ophthalmic clinic for consultation because of legal concerns.