알코올을 처리한 HepG2 세포에서 참죽나무 잎 추출물의 세포 보호 및 항산화 효과

김현정,조수연,김정봉,김헌웅,최정숙,장환희,Kim, Hyun-Jeong,Cho, Su-Yeon,Kim, Jung-Bong,Kim, Heon-Woong,Choe, Jeong-Sook,Jang, Hwan-Hee 한국식품영양학회 2018 韓國食品營養學會誌 Vol.31 No.4

All the parts of the Cedrela sinensis A. Juss., including the seeds, roots, and leaves, have been known to exert medicinal effects. The C. sinensis and its major compound, quercetin, were previously reported to exhibit the anti-inflammatory and anti-oxidative activities. However, the hepatoprotective effects of the C. sinensis leaves against the alcohol-induced oxidative stress in the HepG2 cells have not been studied. In this study, we investigated the antioxidant activities and analyzed the flavonoid contents of the C. sinensis-leaf extract (CE). The total flavonoid contents of the CE is 1,874.5 mg/100 g dry weight (DW), while the total quercetin 3-O-rhamnoside (quercitrin) contents, which was identified as the major flavonol in the CE, is 1,456.0 mg/100 g DW. In the ethanol-stimulated HepG2 cells, the CE effectively prevented the cytotoxic effect and increased the gene expression of the antioxidant enzymes, such as the heme oxygenase-1 (HO-1) and the glutathion peroxide (GPx). The level of the reactive oxygen species (ROS) production was significantly decreased in the CE-treated HepG2 cells. In conclusion, the C. sinensis extract suppressed the alcohol-induced oxidative stress in the HepG2 cells via the induced GPx and HO-1 gene expressions. It is expected the CE positive effects will likely be attributed to the flavonoids, like the quercetin, within the CE.

유아교사 요구에 기초한 장애유아 통합교육 연수프로그램이 교사효능감과 만족도에 미치는 효과

김현정(Kim Hyun-jeong),홍혜경(Hong Haekyung) 한국열린유아교육학회 2007 열린유아교육연구 Vol.12 No.6

본 연구는 유아교사 요구에 기초한 장애유아 통합교육 연수프로그램이 통합교육 교사효능감과 만족도를 알아보는데 있다. 연구의 대상은 연구자가 개발한 장애유아 통합교육 교사 연수프로그램에 참여한 실험집단 22명과 정부기관에서 위탁 통합교육 일반연수에 참여한 통제집단 교사 22명으로, 현재 유아교육현장에서 장애유아를 담당하고 있는 일반유아교사이다. 본 연구의 도구로는 통합교육 교사효능감을 측정하기 위하여 배재정(2005)이 번역하여 사용한 교사효능감의 도구와 김정주(2005), 조광순과 황인갑(2003), 조운주(2004)가 사용한 연수프로그램에 대한 교사의 만족도를 사용하였다. 자료분석은 실험집단과 통제집단간 통합교육 교사효능감의 차이를 비교하기 위하여 통합교육 교사효능감 사전검사 점수를 공변인으로 하고, 사후검사 점수를 종속변인으로 한 ANCOVA를 실시하였으며, 프로그램 만족도의 두 집단 간 차이를 비교하기 위해 t검증을 하였다. 장애유아 통합교육 유아교사 연수프로그램의 효과를 검증한 결과, 통합교육 교사효능감은 실험집단이 통제집단보다 매우 유의하게 높은 것으로 밝혀졌다. 교사효능감의 두 가지 하위영역인 일반적 교사효능감, 개인적 교사효능감에서 있어서도 실험집단이 통제집단보다 매우 유의하게 높은 것으로 나타났다. 또한 본 연구에 참여한 실험집단 교사들은 통제집단 교사들보다 연수에 대한 만족도에서 점수가 높은 것으로 나타났으며, 이러한 차이는 두 집단 간에 통계적으로 유의한 차이가 나타났다. This study examines the effects of a training program of inclusive education for young children with disabilities based on childcare teachers' demands on the teachers' efficacy and satisfaction. The subjects of this study were ordinary childcare teachers at early childhood education institutions executing inclusive education for ordinary and disabled young children. We applied a training program of inclusive education for young children with disabilities to the experimental group of 22 ordinary childcare teachers, and a training program of inclusive education entrusted by institutions to the control group of 22 teachers. In this study, inclusive education teachers' efficacy was measured with the teacher efficacy scale translated by Bae Jae-jeong (2005) and teachers' satisfaction was measured with the scale of satisfaction with training programs used by Kim Jeong-joo (2005), Cho Gwang-soon and Hwang In-gap (2003) and Cho Woon-joo (2004). In data analysis, the difference in inclusive education teachers' efficacy between the experimental group and the control group was analyzed through ANCOVA with the score of pretest on inclusive education teachers' efficacy as a covariate and the score of posttest as a dependent variable. In addition, the difference in satisfaction with programs between the two groups was examined by t-test. According to the results of testing the effect of the training program of inclusive education for young children with disabilities, the experimental group showed significantly higher teacher efficacy than the control group. In the two sub-areas of teacher efficiency - general teacher efficacy and personal teacher efficacy - the experimental group was significantly higher than the control group. In addition, the teachers in the experimental group showed a higher score of satisfaction than those in the control group, and the difference was statistically significant.

Application of NORM to the Multiple Imputation for Multivariate Missing Data

김현정,문승호,신재경,Kim, Hyun-Jeong,Moon, Sung-Ho,Shin, Jae-Kyoung 한국데이터정보과학회 2002 한국데이터정보과학회지 Vol.13 No.2

The statistical analysis of incomplete data sometimes requires handling of incomplete observations. Towards this end, each case with some missing values generally should be deleted, namely, resulting in only use of non-missing cases. EM algorithm(Dempster et al., 1977) which involves prediction and estimation steps is a general method among others. In this article, we use the free software NORM developed for multiple imputation, which uses DA(Data Augmentation) algorithm in its imputation, and evaluate its efficiency through a numerical example.

Propofol Patient-Controlled Sedation Using WalkMed (Medex Inc, USA) Infusion Pump in Dental Patients

김현정,박창주,염광원,Kim, Hyun-Jeong,Park, Chang-Joo,Yum, Kwang-Won The Korean Dental Society of Anesthsiology 2001 Journal of Dental Anesthesia and Pain Medicine Vol.1 No.1

연구배경: 일반적으로 자가통증조절기가 자가진정조절을 위해 적합한 것으로 알려져 있다. 그러나 이 장치들은 몇몇 진정제 투여 시 너무 긴 최소 폐쇄간격을 가지고 있다 WalkMed사(Medex inc, USA)의 자가통증조절기는 폐쇄간격을 0으로 설정할 수 있으며 30 ml/h로 추가용량을 투여할 수 있다. 이번 연구에서는 환자 개개인의 요구에 맞추어 환자의 진정을 조절하기 위하여 위장치를 이용한 propofol 자가진정조절기의 가능성를 조사하였다. 방법: Propofol과 전산 프로그램된 WalkMed 주입장치를 이용한 자가진정조절법이 치과치료를 받는 24명의 건강한 환자에게 시행되었다. Propofol 지속 주입량은 2 mg/kg/h로, 추가용량은 5 mg으로 조절되었으며 치소 폐쇄간격은 0으로 설정하였다. Ketoloac 30 mg이 통증 조절을 위하여 진정법 시행 전에 근주되었다. 결과: 진정법 시행 동안 주입된 propofol의 평균량은 3.4 mg/kg/h이었으며 평균 추가용량은 1.6 mg/kg/h이었다. 시간 당 추가용량에는 많은 변이가 있었다(0-32). 모든 환자는 진정법 시행 동안 완전한 각성상태였으며 이러한 진정 법에 만족하였다. 진정 법과 관련된 주요한 합병증은 관찰되지 않았다. 결론: WalkMed사의 자가통증조절기를 이용한 propofol 자가진정조절법이 치과 치료를 받는 환자들에게 유용하게 사용될 수 있다.

자기주도적 학습능력 촉진을 위한 학업성취도 분석 기반의 수행평가 시스템 구현

김현정,최진식,Kim, Hyun-Jeong,Choi, Jin-Seek 한국정보교육학회 2009 정보교육학회논문지 Vol.13 No.3

The objective of this paper is an implementation of analysing and predicting functions to promote self-directed learning for student's performance assessment system in programming subjects. By adapting Rubric model, the proposed functions inform a student of the assessment criteria and level to be carried out with respects to two-way specifications such as rational ability, problem solving ability and creativity. The proposed system also provides a graphical results of each ability instead of assessment result, for better understanding and analyzing himself/herself based on to the performance assessment and the result. Moreover, the proposed system contains a method to predict future achievement result with moving average technique. Therefore, an academic achievement can be precisely determined by himself/herself to estimate self-directed learning. The teacher can provide different level of educational resources such as supplement learning, problem explains and private instructor etc., in order to maximize efficiency of education.

영지버섯으로부터 homocysteine methyltransferase 를 암호화 하는 metE 유전자의 클로닝 및 E. coli 에서의 발현

김현정,박동철,이갑득,이별라,이갑랑 ( Hyun Jeong Kim,Dong Chul Park,Kap Duk Lee,Byul La Lee,Kap Rang Lee ) 한국균학회 1993 韓國菌學會誌 Vol.21 No.4

벤조피렌을 투여한 생쥐의 간 조직에서의 홍삼분말의 항산화 효과

김현정,이지원,지영주,유미희,박정현,이기동,이인선,Kim, Hyun-Jeong,Lee, Ji-Won,Ji, Young-Ju,Yu, Me-Hei,Park, Jung-Hyun,Lee, Ki-Dong,Lee, In-Seon 한국식품영양과학회 2007 한국식품영양과학회지 Vol.36 No.5

홍삼분말이 $B(\alpha)P$의 투여에 의한 간 독성이 유발된 생쥐에서의 글루타치온 및 과산화지질 함량, 항산화효소 활성에 미치는 영향을 살펴보았다. $B(\alpha)P$ 투여에 의해 증가된 혈청내 ALT와 AST의 활성이 홍삼분말 전 처리군에서는 감소하였다. 간 조직중의 글루타치온 함량은 $B(\alpha)P$ 단독군에서는 감소되었다가 홍삼분말 투여시 유의적인 증가를 보였고, 지질과산화물 함량은 $B(\alpha)P$ 투여시 증가되었다가 홍삼분말의 투여시 유의적으로 감소되었다. 간 조직중의 SOD, catalase 그리고 GSH-Px의 활성도 $B(\alpha)P$ 투여로 유의적으로 증가되었다가, 홍삼분말의 투여로 이들 활성이 유의적으로 감소하였다. 반면, GST 활성은 $B(\alpha)P$ 단독군에서는 감소되었다가 홍삼분말 투여시 유의적인 증가를 보였다. 이상의 결과로 홍삼분말은 항산화효소의 활성에 의한 $B(\alpha)P$에 의한 간 손상에 대한 보호효과를 가지는 것으로 사료된다. The effects of red ginseng powder on hepatotoxicity in $benzo(\alpha)pyrene\;[B(\alpha)P]$-treated mice were investigated. Male ICR mice were pretreated with red ginseng powder (50 or 100 mg/kg/day, for 5 days, intraperitoneally) before treatment with $B(\alpha)P$ (0.5 mg/kg, i.p, single dose). The ability of red ginseng powder to protect against oxidative damage to the mouse liver was examined by determining the level of lipid peroxide, glutathione, and the antioxidant enzyme activities. The glutathione content depleted by $B(\alpha)P$ were significantly increased by red ginseng powder, but elevation of lipid peroxide content induced by $B(\alpha)P$ was decreased by red ginseng powder. The increased activities of superoxide dismutase, catalase and glutathione peroxidase after $B(\alpha)P$-treatment were decreased by the treatment of red ginseng Powder; however, glutathione S-transferase activity depleted by $B(\alpha)P$ were significantly increased. These results suggest that red ginseng powder can protect against $B(\alpha)P$ intoxification through its antioxidant properties.

VAS(Visual Analogue Scale), TTO(Time Trade-Off), EQ-5D(EuroQol-5 Dimension) 방법을 이용한 중증위암의 효용가중치 측정

김현정,이진이,이의경,Kim, Hyun-Jeong,Rhee, Jinn-Ie,Lee, Eui-Kyung 대한약학회 2008 약학회지 Vol.52 No.3

Stomach cancer shows the highest incidence among cancer patients in Korea and it deteriorates the patients' health-related quality of life considerably. This study measured utility weights of severe stomach cancer symptoms using VAS (Visual Analogue Scale), TTO (Time-trade Off) and EQ-5D (EuroQol-5 Dimension) methods. A survey questionnaire was developed to describe the symptoms of severe stomach cancer comprehensively and concisely using VAS, TTO and EQ-5D. A face-to-face interview was conducted for 21 cancer inpatients in a hospital and 25 non-patients. The mean utility weight is 0.498 for VAS, 0.375 for EQ-5D and 0.300 for TTO. Using VAS or TTO methods, there were no statistically significant differences between patients and non-patients while there was significant difference in EQ-5D. The utility weight with VAS and EQ-5D has shown similar trend - higher score in patient group, male and aged while it has opposite trend with TTO.

평면전극구조를 갖는 바이올로진 기반의 일체형 전기변색소자

김현정,이상래,최진희,나윤채,Kim, Hyun-Jeong,Lee, Sang-Rae,Choi, Jin-Hee,Nah, Yoon-Chae 한국분말야금학회 2020 한국분말재료학회지 (KPMI) Vol.27 No.1

Recently, electrochromic devices (ECDs) have gathered increasing attention owing to their high color contrast and memory effect, which make them highly applicable to smart windows, auto-dimming mirrors, sensors, etc. Traditional ECDs have a sandwich structure that contains an electrochromic layer between two ITO substrates. These sandwich-type devices are usually fabricated through the lamination of two electrodes and followed by the injection of a liquid electrolyte in the inner space. However, this process is sometimes complex and time consuming. In this study, we fabricated ECDs with a lateral electrode structure that uses only an ITO substrate and an all-in-one electrochromic gel, which is a mixture of electrolyte and electrochromic material. Furthermore, we investigated the EC properties of the lateral-type device by comparing it with a sandwich-type device. The lateral-type ECD shows strong blue absorption as the applied voltage increases and has a competitive coloration efficiency compared to the sandwich-type device.

족관절 해면골의 미세 구조 분석

김현정,안태선,백명현,최문권,원예연,Kim, Hyon-Jeong,An, Tae-Sun,Baek, Myong-Hyun,Choi, Mun-Kwon,Won, Ye-Yeon 대한족부족관절학회 2004 대한족부족관절학회지 Vol.8 No.2

Purpose: This study aimed to investigate and compare the micro-structural profiles of trabecular bone from different facets at the ankle joint. Materials and Methods: In a fresh cadaver ankle, four cored 10 mm of diameter cylindrical specimens of trabecular bone were harvested from the distal tibia, the talar dome, the medial malleolus, and the lateral malleolus. Using a micro-computed tomography, two-dimensional and three-dimensional micro-structural indices of the trabecular bone were analyzed. Results: Each specimen from the tibia, talus, medial malleolus, and the lateral malleolus showed unique micro-structural pattern. Tibia versus talus, the talus was seen a higher bone volume fraction and a wider supporting zone subchondrally whereas the tibia was seen a relatively lower bone volume fraction and a much narrower supporting zone subchondrally. Lateral malleolus versus medial malleolus, the lateral malleolus was seen the thicker but sparse trabeculae pattern whereas the medial malleolus was seen the thinner but more compact trabecular pattern. Conclusion: Each four locations from the different facet at the ankle joint have distinct own micro-structural patterns of the trabecular bone, suggesting different mechanical properties.