Helicobacter pylori 감염 위상피세포에서 MicroRNA 발현 변화

김창환,김성수,김태호,정우철,김재광,Chang Whan Kim,Sung Soo Kim,Tae Ho Kim,Woo Chul Chung,Jae Kwang Kim 대한소화기암연구학회 2017 Journal of digestive cancer reports Vol.5 No.2

Background: The expression of miRNAs in response to Helicobacter pylori infection has not been well explored. The aims of this study were to evaluate the H. pylori associated miRNAs in the gastric epithelial cells. Methods: We investigated gastric epithelial cell-line (HS3C) exposed H. pylori over 3 months and AGS cell-line (AGS) exposed H. pylori for 6 hour. After the extraction of miRNA from these cell-lines, microarray and real time PCR were performed to confirm the alteration of expression. Results: All 12 miRNAs chosen for real-time PCR are based on the result of microarray and their potential functions related to H. pylori infection. miR-21, miR-221, miR-222 were upregulated in the H. pylori infected AGS cell for 6 hours and HS3C cells. miR-99b, miR-200b, miR-203b and miR-373 were downregulated in the H. pylori infected AGS cell for 6 hours and HS3C cells. miR-23a, miR-23b, miR-125b, miR-141 and miR-155 were upregulated in HS3C cell line but not in H. pylori infected AGS cell for 6 hours. Conclusion: miR-21, miR-99b, miR-125b, miR-200b, miR-203b, miR-221, miR-222, and miR-373 are supposed to be related with oncogenesis of H. pylori infection. Further studies are needed for the evaluation of the function of these confirmed miRNAs.

한국산 산개구리(Rana dybowskii Guenther)의 뇌하수체 전엽에 관한 연구 - II. 동면기와 활동기의 미세구조적 차이

김창환,김우갑,이근옥,김지현,김형배,Kim Chang-Whan,Kim Woo-Kap,Lee Keun-Ok,Kim Ji-Hyun,Kim Hyong-Bai 한국현미경학회 1981 Applied microscopy Vol.11 No.1

활동기와 동면기중 Rana dybowoskii Guenther의 뇌하수체 전엽을 전자현미경적으로 비교한 결과 7 가지의 cell type를 구분할 수 있었으며 이 cell type 중 type 5 에서 많은 차이를 관찰할 수 있었다. Cell type 1 : 분비 과립의 크기는 $375{\sim}687m{\mu}$ 정도였고 모양은 완전 구형이었다. Cell type 2 : 분비과립의 크기는 $250{\sim}437m{\mu}$ 정도였으나 모양은 비교적 다양하였다. Cell type 3 : 분비과립의 크기는 $125{\sim}187m{\mu}$ 정도고 모양은 난형내지 rod 형이었다. Cell type 4 : electron density 가 가장 낮으며 과립의 밀도는 제일 높았다. 모양은 매우 다양하였으며 가끔 대과립도 관찰되었다. 분리과립의 크기는 대개 $210{\sim}420m{\mu}$ 정도이다. Cell type 5 : electron density는 cell type 4 와 유사하나 분비과립의 윤곽은 cell type 4 보다 훨씬 뚜렷하였다. 과립의 밀도는 cell type 4 보다 낮으며 모양은 cell type 4와 비슷하나 rod 형이 많다. 분비과립의 크기는 $200{\sim}863m{\mu}$이다. Cell type 6 : type 2와 비슷하나 cytoplasm 의 electron density가 매우 낮은 light cell 이다. 분비 과립의 크기는 대개 $232{\sim}316m{\mu}$이다. Cell type 7 : 어떤 분비 과립도 함유하고 있지 않은 미분화된 세포이다. 활동기와 동면기의 차이점은 type 5 에서 관찰 할 수 있었다. 즉 활동기에는 분바과립이 매우 발달하였으나 동면기에는 매우 감소되며 cytoplasm에는 크고 작은 vacuole로 가득 차있다. The pars distalis of the Korean frogs (Rana dybowskii Guenther) during hibernating and active periods was observed with the electron microscope. Seven cell types were classified according to the size and shape of secretory granules and to the ultrastructural characteristics. There were many differences between hibernating and active frogs in type 5 cells. Therefore the following results were observed. Cell type 1; This type cell contains spherical secretory granules, $375{\sim}687m{\mu}$ in diameter. Cell type 2; This type cell contains various secretory granules, $250{\sim}437m{\mu}$ in diameter Cell type 3; Spherical and rod-shaped granules, $l25{\sim}187m{\mu}$ in diameter were observed. Cell type 4; In this type cell, the electron density is the lowest and the density of granules is the highest of all type cells. This type cell contains various secretory granules and large secretory granules, $2l0{\sim}420m{\mu}$ in diameter, were also observed. Cell type 5; The electron density of this cells is similar to that of type 4 cells. The density of granules is lower than that of type 4 cells. And the shapes of the secretory granules are similar to those of type 4 cells. But many rod shaped granules, $200{\sim}863m{\mu}$ in diameter, were also observed. Cell type 6; This type was similar to type 2. The electron density of cytoplasm is very low. Spherical granules, $232{\sim}316m{\mu}$ in diameter, were observed. Cell type 7; This type of cell has no secretory granules. This cell is not developed very well. The type 5 cells in hibernating frogs are different from cells in active frogs. In type 5 cells, many secretory granules were observed during active period. But the number of secretory granules were greatly declined and there were many vacuoles in cytoplasm during hibernating period.

Relationships between the Host and it's Natural Enemy Introduced into a New Ecosystem 1. Mortality of Hyphantria cunea Drury killed by Podisus maculiventris

김창환,노용태,정영화,Kim, Chang-Whan,Noh, Yong Tai,Chung, Young Wha The Korean Society for Integrative Biology 1969 동물학회지 Vol.12 No.4

容接이 103,341$cm^3$의 鐵製網室에 Platanus sp.의 切樹를 植栽하고 1區를 3組씩으로 하여 H. cunea 의 幼蟲과 天敵의 比를 200:10, 400:10, 800:10 그리고 天敵을 投入하지 않은 對照區를 두어 H. cunea 의 P. maculiventris에 依한 被殺率을 化期別로 調査하였다. 1. H. cunea의 一化期에서 200:10 區는 94.6%, 400:10 區는 91.4%, 800:10區는 62.4%의 H. cunea의 被殺率을 보여주었다. 2. H. cunea의 二化期에서 200:10區는 96.3%, 400:10 區는 93.6%, 800:10區는 67%의 H. cunea의 被殺率을 보여주었다. 3. 對照區의 境遇는 H. cunea의 一化期에서 98.7%, 二化期에서 94.4%의 H. cunea가 幼蟲世代를 마쳤다. The present experiment was designed to learn the mortality (response per predator) of Hyphantria cunea and Podisus maculiventris, both reared in different ratios of densities in net cage. 1. Podisus maculiventris attacked about 94.6, 91.4 and 62.4% of the hosts at the ratio of densities, 200:10, 400:10 and 800:10 in each net cage in the lst generation. 2. Podisus maculiventris attacked about 96.3, 93.6 and 67% of the hosts at the ratio of densities, 200:10, 400:10 and 800:10 in each net cage in the 2nd generation. 3. About 98.7 and 98.4% of the hosts were pupated in the 1st and 2nd generations in the controlled group.

배추흰나비 (Pieris rapae L.) 의 생식소에 관한 연구 I. 정소의 전자현미경적 연구

김창환,김우갑,한성식,이근옥,Kim Chang-Whan,Kim Woo-Kap,Han Sung-Sik,Lee Keun-Ok 한국현미경학회 1981 Applied microscopy Vol.11 No.1

Testis of Pieris rapae L. was observed by using light microscope and electron microscope. The results were as follows 1. Pieris rapae L. possesses a ovoid single testis colored red. 2. Peritoneal sheath consists of three layers; outer cuticular layer, middle filamentous layer, inner layer with no filaments. 3. Follicles enclosed by peritoneal sheath are compacted by the folds of the. epithelium of follicle. They are opened together toward the duct of vas deferens. 4. Epithelium of follicle have high electron density and contain needle shaped structures and glycogen. Tracheoles are extended into the epithelium of follicle. 5. Cyst cells have a irregular shaped nucleus and cell organelles are abundant in cytoplasm. 6. Typical acrosomes were not observed, but the other structure smillar to the acrosome of the other insect or vetebrate were observed. 7. After releasing the sperm bundle, cyst cells seem to be autolyzed. 배추흰나비 (Pieris rapae L.)의 정소를 적출하여 광학현미경과 전자현미경으로 관찰하여 다음과 같은 결과를 얻었다. 1. 성숙정소는 1 개이었고 난형이었으며 붉은 빛을 띄고 있었다. 2. 복막초는 3 개의 층으로 이루어져 있었는데 가장 바깥층은 큐티클층이었고 그 안쪽으로 필라멘트가 관찰되는 층과 필라멘트가 관찰되지 않는 층으로 관찰되었다. 3. 여포는 복막층에 의해 둘러싸여 있었는데 여포는 여포상피에 의해 나뉘어져 있었으며 또한 이는 수정관쪽으로 개구되어 합쳐져 있었다. 4. 여포상피에는 기관 (氣管)이 들어와 있었고 침상형의 구조물과 글리코겐과립이 다수 관찰되었고 전체적으로 전자밀도가 높았다. 5. 피낭세표의 핵의 모양은 불규칙하였고 세포소기관은 비교적 풍부하였다. 6. 전형적인 침체는 관찰되지 않았으나, 다른 곤충이나 고등동물의 침체와 유사한 구조가 관찰되었다. 7. 피낭세표는 정자를 방출하고 난 뒤에 분해를 일으키는 것으로 사료된다.

배추흰나비(Pieris rapae L.)의 위심세포(圍心細胞)에 관한 연구

김창환,김우갑,이근옥,Kim, Chang-Whan,Kim, Woo-Kap,Lee, Keun-Ok 한국현미경학회 1989 Applied microscopy Vol.19 No.1

The oval shaped pericardial cells are clustered along the lateral sides of the heart and irregularly connected with the heart. The cells are bounded by a basement membrane. The basement membranes of the connected two peicardial cells are irregularly linked each other there-fore funnels are formed. The multiple invaginations of the cell membrane are observed and septate junctions develope at the part of enterance of the cell membrane. The coated pits are appeared in the inner side of the invaginated cell membrane. The coated vesicles, tubular and spherical shaped vesicle, Golgi complex containing high electron densed material in the cisternae and mitochondria are observed in the cytoplasm and lysosomes are remarkably well developed. The whirled membrane structures in the multiformed complex bounded by single membrane are linked with low electron densed granules and spherical shaped small granules having high electron density with $0.03{\mu}m$ in diameter are located between the whirled membrane in a row and gradually secretes the granules and then they produced the multilamellar body. The lysosomal regions of cytoplasm of pericardial cell are appeared negative reaction to the acid phosphatase and according to the results of the electrophoresis, lipoproteins having acid phosphatase activity are contained. The axon is contacted with the pericardial cells.

Tremex apicalis Matsumura as a Host of an Ichneumon-fly, Megarhyssa jezoensis Matsumura

김창환,Kim, Chang-Whan The Korean Society for Integrative Biology 1971 동물학회지 Vol.14 No.2

Kim and Son (1969) reported an ichneumon-fly, Megarhyssa jezoensis Matsumura, as a parasite of Sirex nitobei Matsumura which is one walnut, Juglans sinensis Maximowicz but Sirex nitobei Matsumura was misdetected by them. The host of Megarhyssa jezoensis Matsumura is not Sirex nitobei Matsumura but Tremex apicalis Matsumura which was unrecorded in Korea up to the date. And the fact is added that Ibalia takachihoi Yasumatsu also attacks the same pest, Tremex apicalis Matsumura.

Megarhyssa jezoensis Matsumura (에조납작맵시벌)의 生活史에 關하여

김창환,손제태,Kim, Chang-Whan,Son, Je Tae The Korean Society for Integrative Biology 1969 동물학회지 Vol.12 No.1

忠南 禮山郡 禮山邑, 新陽面, 大興面의 3地區에서 1964年 4月부터 1968年 12月까지 調査한 結果 Megarhyssa jezoensis가 호도나무의 害蟲인 Sirex nitobei에 寄生함을 알았고 그 生活史는 다음과 같았다. 1. 越冬後 成蟲出現은 4月下旬부터 5月中旬까지이며 最盛期는 5月 5日이였고, 一化基成蟲은 7月上旬에서 7月中旬 사이에 出現했고 最盛期는 7月 10日 이였으며 二化基成蟲은 9月下旬부터 10月下旬까지에 出現했고 最盛期는 10月 8日 이였다. 2. 幼蟲發育은 一化基가 5月上旬부터 6月下旬까지 二化基가 7月中旬부터 9月上旬까지, 三化基는 10月上旬부터 이루어졌다. 3. 期는 6月下旬부터 7月中旬까지이고 二化基는 8月下旬부터 9月中旬까지이다. 4, 成蟲은 호도나무 줄기속 Sirex nitobei의 成蟲에 産卵하고 孵化幼蟲은 Sirex nitobei의 幼蟲을 파먹고 成長 化한다. 態로 越冬하되 이른 것은 早春에 成蟲으로 되어 호도나무 줄기속에 머물러 있다. An ichneumon-fly, Megarhyssa jezoensis Matsumura, was found as a parasite of Sirex nitobei Matsumura, a pest insect of the walnut, Juglans sinensis Maximowicz, at Ye-San, Choong-Nam province, middle Korea. The life cycle of Megarhyssa jezoensis was as follows: 1. It occurred three times a year. The adults appear at the end of April to the middle of May (maximum on 5th May) after hibernation, at the beginning to the middle of July (maximum on 10 th July) in the first generatioin, and at the end of September to the end of October (maximum on 8 th October) in the second. 2. The larvae grew from the beginning of May to the end of June in the first generation, from the middle of July to the beginning of September in the second and from the beginning of October to the end of the year in the last. 3. The pupae developed from the end of June to the middle of July and from the end of August to the middle of September. 4. The adults oviposited their eggs on the surface of the host, the larvae in Sirex nitobei growing in the stem of the walnut, and the larvae hatched from the eggs were ectoparasiting the larvae of Sirex to grow and pupate. The parasite hibernated in the pupal stage but the adult which emerged at the early spring stayed in the hole of the walnut stem till the end of April.

한국인 바렛식도에서 p53과 Ki-67 단백의 면역조직화학 발현

김창환 ( Chang Whan Kim ),이보인 ( Bo In Lee ),김병욱 ( Beung Wook Kim ),김진일 ( Jin Il Kim ),박수헌 ( Soo Heon Park ),김재광 ( Jae Kwang Kim ),한석원 ( Sok Won Han ),정인식 ( In Sik Jung ),선희식 ( Hee Sik Sun ),이아원 ( A Won L 대한소화기학회 2005 대한소화기학회지 Vol.46 No.3

목적: 바렛식도는 식도선암의 전구병변으로 원주상피로 치환된 식도상피의 특수장상피화생에서 이형성이 존재할 경우 식도선암의 발생과 관련이 깊다. 최근 한국에서 역류 식도질환이 증가하고 있지만 아직 바렛식도와 식도선암의 유병률은 서구에 비해 현저히 낮다. 이에 저자들은 바렛식도의 암성 변화의 예측에 유용한 p53과 Ki-67단백의 면역조직화학 발현 양상을 알아보고자 하였다. 대상 및 방법: 상부위장관 내시경검사에서 원주상피로 치환된 식도상피의 변화가 관찰되어 바렛식도의 확진을 위해 식도 조직검사를 시행한 95명을 대상으로 하였다. 특수장상피화생의 진단을 위해 모든 생검 예에서 alcian blue 염색(pH 2.5)을 시행하였고, 장형화생의 유무를 관찰하기 위해 high ion diamine 염색을 하였다. 그리고 전 예에서 p53과 Ki-67 면역조직화학 검사를 시행하였다. 결과: 내시경검사에서 바렛식도가 의심되었던 95명 중 55명(57.9%)에서 특수장상피화생이 관찰되었고 전 예에서 이형성은 없었다. 이들 55명 중 31명(56.4%)에서 sulfomucin 양성인 장형화생이 관찰되었다. 바렛식도로 조직 진단을 받은 55명 중 6명(10.9%)에서 p53 단백질의 핵내 침윤소견이 관찰되었고 이들 6명 모두에서 장형화생이 존재하였다. Ki-67 표지지수는 특수장상피화생이 있거나 p53 양성일 때 상대적으로 높게 나타났으나 유의성은 없었다. 결론: 바렛식도 환자에서 p53 단백질이 발현된 경우는 10.9%로 이들 모두에서 장형화생을 동반하고 있어 한국인에서도 바렛식도는 이형성의 발생이나 암성변화의 위험도가 높은 전암병소이며 향후 이들에 대한 추적 검사가 필요하다. Background/Aims: Barrett`s esophagus is a premalignant lesion of the esophagus in which normal squamous epithelium is replaced by intestinalized columnar epithelium. In Korea, adenocarcinoma associated with Barrett`s esophagus is rare compared with that of Western country. The purpose of this study was to investigate the immunohistochemical expression of p53 and Ki-67 in Barrett`s esophagus which had predictive value for cancer risk in Korea. Methods: Ninety five patients (43 male and 52 female, median age 44, range 21-75) who have been suspected to have Barrett`s esophagus by endoscopic assessment were enrolled in this study. Alcian blue (pH 2.5) and high ion diamine stain for the evaluation of specialized intestinal metaplasia (SIM) and immunohistochemical stain for p53 and Ki-67 were done. Results: 57.9% (55/95) of biopsies from the columnar lined esophagus showed SIM, but no dyspalsia. 56.4% (31/55) of Barrett`s esophagus showed sulfomucin positive colonic metaplasia. The p53 expression was observed in 10.9% (6/55) of the patients of Barrett`s esophagus and all of them showed colonic metaplasia. Ki-67 labeling index showed no difference significantly. Conclusions: In Korea, 10.9% of Barrett`s esophagus had p53 mutation and moreover all of them had colonic metaplasia. Consequently, we expect that these patients have high risk of developing dysplasia and adenocarcinoma and need careful follow-up. (Korean J Gastroenterol 2005;46:189-195)

창립 20주년 기념호 : 기념호창간사

김창환(Chang Whan Kim) 고려대학교 한국곤충연구소 1983 昆蟲硏究誌 Vol.9 No.1

Platelete aggregation test 및 P.R.P 분리에 관하여

김창환 ( Chang Whan Kim ),김학근 ( Hak Geun Kim ) 대한임상검사과학회 1982 대한임상검사과학회지(KJCLS) Vol.14 No.1

The well-initiated platelete-rich-plasma in the blood coagulation mechanism will be able to bring the platelete aggregation test in good shape, and eventually the test can be a monitoring indices for the quality control in the hematology. It was found that some essential factors, such as blood collection, container med, PRP isolation and storage of the blood, etc., played an indispensable role in the test procedures.