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윤경원(YOON Kyung Won),김강련(KIM Kang Ryune),우재형(WOO Jae Hyung),김진정(KIM Jin Jeong),김재봉(KIM Jae Bong) 대한체질인류학회 1989 해부·생물인류학 (Anat Biol Anthropol) Vol.2 No.1
한국 성인의 정상 하악골 114예에서 이공에 대한 해부학적 위치를 조사 분석한 결과 다음과 같은 결과를 얻었다. 1. 하 악치에 대한 이공의 상대적인 위치는 이공이 제2소구치의 근연(root edge)에 위치하는 경우(type IV) 가 가장 높은 빈도를 보였으며 제1소구치와 제2소구치의 사이에 위치하는 경우(type Ⅲ)와 제2소구치와 제1대구치의 사이에 위치하는 경우 (type V)가 각각 다음 순서를 차지하였다. 제1소구치보다 앞쪽이나 제1 소구치의 근연 및 제1대구치의 근연 뒤쪽 에 위치하는 경우(type I, Ⅱ, Ⅳ)는 관찰 되지 않았다. 2 하악골 체의 상연과 하연으로부터의 거리는 그 비가 약 l.2 : l로서 이공은 하연 쪽으로 치우쳐 있었으며 상연에서 하연까지의 길이는 좌측에서 31.09±2.80mm, 우측에서 30.97±2.48mm 이었다. 3 하악결합에서 이공의 전연까지의 길이는 좌측에서 29.52±2.01mm, 우측에서 30.82±2.04mm이었고, 하악결합에서 하 악치의 후연까지의 길이는 좌측에서 l04.20±4.74mm, 우측에서 105.44± 4.49mm로서 이공은 하악결합에서 하 악치 후연까지 길이의 앞쪽 약 1/4에 위치하는 것으로 조사 되었다. 4 하악 제2소구치조의 바닥으로부터 이공의 상연까지의 길이는 좌측에서 5.46±3.09mm, 우측에서 5.73±3.03mm로 양의 값을 나타냄으로써 하악 제2소구치조의 근첨(root apex)이 이공의 상연보다 윗 쪽에 존재하였다.
천명규(Myoung-Kyu Cheon),문용석(Yong-Suk Moon),허은영(Eun-Young Huh),홍혜남(Hae-Nam Hong),백선용(Sun-Yong Baek),김재봉(Jae-Bong Kim),김진정(Jin-Jeong Kim),김봉선(Bong-Seon Kim) 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.2
임신당일부터 임신 3주까지 임신기간의 경과에 따른 흰쥐 질점막상피의 구조를 광학현미경 및 전자현미경으로 관찰하였 고, 또한 임신기간의 흰쥐 질점막상피속에 존재하는 복합당질의 당잔기를 알아보기 위하여 lectin 반응을 시행하였다. 암 흰쥐를 숫 흰쥐와 합사시킨 다음날 질액도말표본 (vaginal fluid smear)에서 정자가 발견된 날을 임신당일로 하였다. 임신당일에 불규칙한 다각형을 하고 있는 바닥부분의 세포를 제외하고는 대부분의 세포들이 납작한 형태를 하고 있었으 며 위쪽 2 ~ 3층은 세포질이 엉성하게 배열되어 있는 밝은 세포들로 구성되어 있었다. 모든 세포들은 세포질중간세사다발을 많이 가지고 있었고 세포막에서 미세융모와 같은 돌기가 돌출되어 있으며 이웃세포막의 돌기와 접촉하고 있는 곳에서는 desmosome이 발달되어 있었다. 점액세포는 임신 2일부터 소수 나타나서 임신기간이 경과할수록 점액세포의 숫자와 두께가 증가하였다. 점액세포는 다각형에서 키가 큰 원주형으로 점차 변하였다. 세포질중간세사다발들이 임신1주까지의 점액세포내에서는 많이 관찰되었으나 임신 2주와 임신 3주에서는 드물게 관찰되었다. 점액세포의 점액질과 바닥 세포들의 복합당질은 임신기간에 따라 정도의 차이는 있었으나, 점액세포는 α-L-fucose, α-Dgalactose, β(1, 4)-N-acetylglucosamine, α-D-N-acetylgalactosamine 및 galactosyl-β(1, 4)-N-acetylglucosamine을 함유하고 있었으며, 바닥부분의 세포들은 galactosyl-β(1, 4)-N-acetylglucosamine을 제외한 당잔기를 함유하고 있었다. 흰쥐 질점막상피의 점액층의 두께가 출산이 가까워 올수록 두터워지는 것은 점액층에 존재하는 복합당질을 함유하고 있 는 점액질이 임신과 출산에 적합한 질 내강 환경을 만드는데 관여 할 것으로 생각된다. The present study is designed to study the light and electron microscopic structure of the rat vaginal epithelium during pregnancy. Furthermore, lectin histochemistry is used to know the changes of the terminal sugar of the glycoconjugate in the vaginal epithelium during pregnancy. The 0 day of pregnacy is defined as the day of presence of sperm in a vaginal smear. At 0 day of pregnancy, most of cells are flat in morphology except basal cell. Lightly-stained superficial cells had few cell organelles, and all the other cells had many intermediate filament bundles, microvilli-like processes and desmosomes. During pregnancy, the thickness of the vaginal epithelium was increased. The morphology of the mucous cells are changed from a cuboidal shape to a columnar one. The intermediate filament bundles are decreased in the mucous cell after first week of pregnancy. Lectin histochemistry showed the presence of α-L-fucose, α-D-galactose, β (1, 4)-N-acetylglucosamine, α-D-Nacetylgalactosamine and galactosyl-β (1, 4)-N-acetylglucosamine in the mucous cells. The basal cells also contained the same terminal sugars except galactosyl-β (1, 4)-N-acetylglucosamine. Approaching to the birthday, the thickening of the mucous layer of the vaginal epithelium suggests that the mucous containing several glycoconjugates may play an important role to make the appropriate environment in the vaginal lumen during pregnancy and parturition.
흰쥐의 장관내 점액질 성상에 미치는 Bassa 살충제의 영향에 관한 조직화학적 연구
김진정,조운복 부산대학교 1981 자연과학논문집 Vol.31 No.-
This experiment was performed to observe the effect of carbamate pesticide(Bassa) on the production, secretion, and properties of the mucosubstances in the duodenal glands, and the goblet cells of the duodenum, ileum, proximal colon, distal colon and rectum with histochemical methods. Healthy adult rats weighing about 250~300g each were divided into two groups-normal and experimental. Experimental groups each were administered Bassa, and sacrificed in 1,3,6,12,24,48, and 72 hours after administration of the pesticide. The mucosubstances were stained with alcian blue pH 2.5, alcian blue pH 1.0, alcian blue pH 2.5 - periodic acid Schiff(PAS), alcian blue pH 1.0 - PAS or aldehyde fuchsin pH 1.7 - alcian blue pH 2.5. The obtained results were as follows: 1. In the small intestine, the amount of neutral mucins in the duodenal glands were most remarkable in 6 and 12 hours with Bassa. Amounts of neutral mucins in diminution were gradually recovered from 48 hours after administration. The diminution of mucosubstances in the goblet cells of the small intestine after administration was more noted in upper villi and lower crypts, and decrease of acid mucins was more notable than that of nuetral mucin. That is, the diminutions of acid mucins were most remarkable in 1 and 3 hours (ileum), and 1 to 12 hours (duodenum) after administration. The diminished acid mucins in duodenum and ileum started to recover from 48 hours after administration. The diminution of neutral mucins were most remarkable in 6 and 12 hours (duodenum), and were marked in 1 to 6 hours (ileum) after administration. The gradual recovery of nuetral mucins in 24 hours (duodenum) and 12 or 24 hours (ileum) after administration was noted. In villi and crypts of the small intestine after Bassa administration, goblet cells secreting strong sulfated mucin and goblet cells secreting nonsulfated mucin both were severely decreased staining properties, and the formers were more severely effected than the latters. The recovery of the former goblet cells was more delayed than the latters. And this pesticide was more severely effected to the duodenum than ileum. 2. In the large intestine, the amount of mucosubstances in the goblet cells after administration was more noted in surfaces and lower crypts, and decrease of acid mucins was more notable than that of neutral mucin, that is the diminution of acid mucins were most remarkable in 1 hour (rectum), 1 and 3 hours (distal colon), and 1 to 6 hours (proximal colon) after administration. The recovery of acid mucins in 12 hours (distal colon and rectum), and in 48 hours (proximal colon) after administration was noted. The diminution of neutral mucins were most remarkable in 1 hour (distal colon) and 1 to 6 hours (proximal colon), and were marked in 1 to 6 hours (rectum) after administration. Amount of neutral mucins in diminution were gradually recovered from 12 hours (distal colon and rectum) and 24 hours (proximal colon) after administration. In surfaces and crypts of the large intestine after Bassa administration, goblet cells secreting strong sulfated mucin and goblet cells secreting nonsulfated mucin both were decreased staining properties, and the formers was more remarkable than the latters, furthermore this pesticide was more severely effected to the proximal colon than distal colon and rectum. On the basis of the above results, it is suggested that were severely inhibited to production and secretion of acid and neutral in earlier time period of the experiment, and the production of acid mucins were severely inhibited more than neutral mucin. In comparison with the small and large intestine, the small intestine seems to be inhibited more than the large intestine. In comparison with organs, the duodenum seems to be inhibited more than ileum in small intestine, and the proximal colon more than distal colon and rectum in large intestine.